Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

53895894 .. 53906262

Gene Structure 10369 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00036920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGATTGCGGAGAAGCCCGGTTGGATTAAGCACGAAGGACTGCAGATATTCTCCATTGATGTTCAGCCCGGTGGGCTTAGGCTTGCCACCGGTGGGGGCGATCACAAGGTGCGAATATGGAACATGAAATCTCTTGGCCGGGATATGGAAAATGAGGACTCAACTCAGAGGCTACTTGCAACCCTCCGTGATCACTTTGGTTCTGTTAACTGTGTTAGGTGGGCTAAGCACAGTCGTTATGTTGCAACAGGATCTGATGATCAAGTGATTCTAATTCATGAGAGGAAGCCTGGTTCGGGAACCACTGAGTTTGGCAGTGGAGAGCCCCCTGATGTTGAGAATTGGAAAGTTATAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTTAATTGGTCTCCAGATGACTCGATGTTGGCCAGTGGAAGTTTAGATAATACTATACATATCTGGAATATGAGCAATGGTATTTGCACTGCTGTTCTTAGGGGTCACTCTAGCCTGGTTAAAGGAGTTACTTGGGATCCCATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACAGTAATTATTTGGAAGACAAGCGACTGGAGTCTTGCTCACAGGACTGACGGTCACTGGGCAAAATCACTTGGATCTACCTTTTTCAGGCGTCTTGGATGGTCACCTTGTGGTCACTTCATTACTACTACTCATGGATTCCAGAAACCAAGGCATTCAGCACCTGTTCTTGAGAGAGGCGAATGGTCTGCCACATTTGACTTCCTAGGACATAATGCTCCTATAATCGTTGTGAAGTTTAACCATTCGATGTTTATGAGGAAGTTTGCCAATGCTCAGGAAGGGAAAACTGCACCTGTGGGGTGGACTAATGGGGCTTCGAAGACAGGAGGGAAAGAACCACAACCTTATAATGTTATTGCAATTGGGAGTCAGGACCGGACAATAACTGTATGGACGACTGCAAGCCCACGTCCTCTATTTGTTGCAAAGCATTTCTTTACTCAAAGTGTTGTGGATTTGTCCTGGAGCCCTGATGGTTATTCGCTCTTTGCATGTTCTTTGGATGGTTCGGTGGCTACTTTCCATTTTGAGGTGAAAGAACTTGGTCACCGGTTAAGTGATGCAGAATTAGATGAGTTGAAGAGAAATCGTTATGGTGATGTTAGAGGTCGGCAAGCAAACTTAGCTGAAAGCCCAGCACAGTTATTGCTTGAAGCAGCTTCTGCTAAGCAAGCGGCAAGCAAAAAGGTCTCTCTGGATGTTCAACAAAATCACACACTTGAGAAACCTTCTGCTCATGTGGGGGTTGCAACAAAAGCTTCTGAGACTCAGCTTGCTGATATGAAAAAGAGTGGAGGTGCTGCTGCTGATTGTTTAAATAAAGTTTCGATGGCTACCCGAATGTCTGGTCCTGTGAAGCAAAGAGAATATAGACGCCCTGATGGTAGAAAAAGGATTACTCCAGAAGCAGTTGGGGTGCCTTCCCGGCAGGAAAATATCTCTATGGGGGCTCAGTCCCAAGCACTTGACTTCCCTCCCATGTCTTCTGATCATAGGAAGGATGATAATGGTTTAGTTGTTGCTGATAGTGGCATTAGAGAAACTTCTTTCAGGGGAACACTTGGCCGAAACACTGATACAAAGGAAAGATATGGGGCCACTGCCAGAGCCATGATTACTGAAAGTCTGGTTATTGAGAAGGTTCCTGCTTCCACAAGCAGAGATGAAAGCATAAATGTGGAACAAACGGGAAATGTGAAGGCGTGTAACTCAGTAGGCTCTAGTAGTTCTATTCTTTCAATCCGGGTGTTCGATAAGAAAGAATGGGAAGACACAGTGCCAATTTGTTTGGAAGCCCGACCTTGGGAACAAGCTGCAAATGATGTGTTTGGCATGGGAAATACATTTATGACGAAAGAAACTGAAATCACTTGCACAAGAGGGTTGCAAACTCTTTGGTCTGATAGGATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGTAAACTTTTGGGCTGTTGGCTGTGAAGATGGTTGCATACAGGTTTACACAAAGTGTGGGAGACGTGCTATGCCAACAATGATGGTAGGATCTGCTGCAATATTTATAGATTGTGACGAGTGCTGGAAGTTGTTTTTGGTCACAAGAAAAGGATCCTTGTATCTGTGGGATCTATTCACACGGAACTGTCTCCTTAATGACTCCTTGGCATCTCTAGTTACTTCAAACCAGAACCCATCTGCAAAAGATGCAGGCATAATCAAAGTTATATCAGCAAAACTATCAAGATCTGGATCACCTATTGTTGTTCTTGCGACTCGTCATGCATTCCTCTTCGACATGGGCCTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTCCTGGGTCAAATTTTGCAAGCTCCTGGAATTTAACTTCAACTCAGAGTGGCGAGCTGGCTACATTGCAGGTTGATGTTAGGAAATATTTGGCCAGGAAGCCGGTTTGGAGCAGGGTGACTGATGACGGAGTACAGACACGTGCTCATTTGGAAGCTCAGTTGGCTTCTTCACTGGCTTTGAAGTCCCCTTATGAATATCGCCAATGTCTCCTATCATACATACGCTTCCTCGCAAGAGAAGCAGATGAGTCCCGTTTGCGAGAAGTCTGTGAGAGTTTTCTTGGACCTCCTACTGGGATGATGGAAAATACATCTTTACATTCGAAGAACTTAGCATGGGATCCTTGTGTCCTTGGAATGAGGAAACATAAACTTTTACGAGAAGACATTCTTCCAGCAATGGCTTCAAATAGGAAAGTACAACGACTGCTTAATGAATTCATGGATCTCATTTCTGAATATGAAAGTGTTGAAACACATACAGAGAAAAAATCTCACGCTTCACCAACAGAGAATGCACCGGCAGCTGAATTAATGGACTCTTCCCCAACGGTGACAAATCAACTGAACTTGGTATCTGCACCAACAGATCAAATGAAGTCTGTTCATGCTTCAACTGATATAGTGGACTCCTCCCAGGTAGCATTAAATCAAGAAAATTCCGCTCCACTAGCTGAAGATAAATTTGATTCAGATCAACCACCACCAGTGATTGATCAAGTTAGTCTTACCTCACAAGACGCAGGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

1047

Amino Acids

115.26

Weight (kDa)

6.65

Isoelectric Point (pI)

36.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 371	1.4e-54	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	14 - 97	4.3e-06	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	15 - 199	2.1e-33	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	16 - 99	1e-08	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 201	1.6e-16	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	22 - 193	2.2e-11	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	54 - 262	1.4e-20	CDC20/Fizzy WD40 domain
Beta-prop_Aladin	PF25460	67 - 231	2.4e-09	Aladin seven-bladed propeller
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	3.4e-08	WDR35/TULP4 N-terminal
Beta-prop_WDR3_1st	PF25173	111 - 211	4.2e-18	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	111 - 198	2.1e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	111 - 230	1.6e-19	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 195	3.3e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	112 - 208	4.3e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	115 - 210	1.2e-09	WDR3 second beta-propeller domain
WD40	PF00400	115 - 153	1.8e-10	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	123 - 258	3.7e-15	WDHD1 first WD40 domain
WD40	PF00400	158 - 194	2.4e-06	WD domain, G-beta repeat
Hira	PF07569	690 - 889	2.2e-50	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	353, 918
Acc36I	ACCTGC	2 cut(s)	2449, 3125
AccB1I	GGYRCC	1 cut(s)	1486
AccBSI	CCGCTC	1 cut(s)	3056
AciI	CCGC	3 cut(s)	8, 1244, 3054
AcoI	YGGCCR	4 cut(s)	136, 420, 1633, 2481
AcsI	RAATTY	5 cut(s)	2401, 2419, 2828, 3049, 3074
AcuI	CTGAAG	1 cut(s)	3087
AcvI	CACGTG	1 cut(s)	2531
AcyI	GRCGYC	2 cut(s)	658, 1444
AdeI	CACNNNGTG	3 cut(s)	109, 677, 2064
AfaI	GTAC	2 cut(s)	2523, 2811
AfiI	CCNNNNNNNGG	8 cut(s)	93, 145, 296, 654, 1099, 1872, 1873, 2684
AflIII	ACRYGT	1 cut(s)	2528
AgeI	ACCGGT	2 cut(s)	89, 1119
AhdI	GACNNNNNGTC	1 cut(s)	618
AjiI	CACGTC	2 cut(s)	980, 2075
AjnI	CCWGG	7 cut(s)	289, 504, 1031, 2392, 2414, 2483, 3027
AjuI	GAANNNNNNNTTGG	2 cut(s)	2129, 2161
AloI	GAACNNNNNNTCC	2 cut(s)	277, 309
Alw21I	GWGCWC	1 cut(s)	2536
Alw26I	GTCTC	6 cut(s)	405, 1264, 1328, 2065, 2204, 2603
AlwNI	CAGNNNCTG	3 cut(s)	254, 731, 1232
AoxI	GGCC	6 cut(s)	136, 420, 1633, 1665, 2353, 2481
ApeKI	GCWGC	6 cut(s)	1226, 1370, 1373, 1883, 2105, 2915
ApoI	RAATTY	5 cut(s)	2401, 2419, 2828, 3049, 3074
ArsI	GACNNNNNNTTYG	2 cut(s)	1402, 1434
AseI	ATTAAT	1 cut(s)	2924
AsiGI	ACCGGT	2 cut(s)	89, 1119
Asp700I	GAANNNNTTC	1 cut(s)	2778
AspA2I	CCTAGG	1 cut(s)	772
AspS9I	GGNCC	5 cut(s)	943, 1418, 1665, 2353, 2675
AsuC2I	CCSGG	5 cut(s)	18, 69, 140, 1495, 1814
AsuHPI	GGTGA	8 cut(s)	663, 1109, 1114, 1178, 2298, 2518, 2886, 2956
AsuII	TTCGAA	2 cut(s)	887, 2714
AvaII	GGWCC	3 cut(s)	943, 1418, 2675
AvrII	CCTAGG	1 cut(s)	772
BalI	TGGCCA	2 cut(s)	422, 2483
BamHI	GGATCC	3 cut(s)	526, 2162, 2731
BanI	GGYRCC	1 cut(s)	1486
BanII	GRGCYC	3 cut(s)	327, 1040, 1522
BbrPI	CACGTG	1 cut(s)	2531
BbsI	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2781
Bbv12I	GWGCWC	1 cut(s)	2536
BbvI	GCAGC	6 cut(s)	1238, 1357, 1360, 1870, 2092, 2927
BccI	CCATC	9 cut(s)	660, 1037, 1067, 1393, 1445, 2033, 2086, 2254, 2686
BcgI	CGANNNNNNTGC	2 cut(s)	737, 771
BciT130I	CCWGG	7 cut(s)	291, 506, 1033, 2394, 2416, 2485, 3029
BclI	TGATCA	4 cut(s)	190, 259, 1558, 3106
BcnI	CCSGG	5 cut(s)	18, 69, 140, 1495, 1814
BcoDI	GTCTC	6 cut(s)	405, 1264, 1328, 2065, 2204, 2603
BfaI	CTAG	5 cut(s)	501, 773, 1791, 2225, 3062
BfmI	CTRYAG	2 cut(s)	41, 375
BfuAI	ACCTGC	2 cut(s)	2449, 3125
BglI	GCCNNNNNGGC	2 cut(s)	73, 1495
BglII	AGATCT	1 cut(s)	2297
BisI	GCNGC	7 cut(s)	1227, 1245, 1371, 1374, 1884, 2106, 2916
BlnI	CCTAGG	1 cut(s)	772
BlpI	GCTNAGC	2 cut(s)	225, 1236
BlsI	GCNGC	7 cut(s)	1228, 1246, 1372, 1375, 1885, 2107, 2917
Bme18I	GGWCC	3 cut(s)	943, 1418, 2675
BmeRI	GACNNNNNGTC	1 cut(s)	618
BmgBI	CACGTC	2 cut(s)	980, 2075
BmgT120I	GGNCC	5 cut(s)	943, 1418, 1665, 2353, 2675
BmiI	GGNNCC	9 cut(s)	301, 528, 538, 1037, 1488, 1666, 1713, 2164, 2733
BmrI	ACTGGG	2 cut(s)	634, 2694
BmsI	GCATC	3 cut(s)	1120, 2228, 2248
BmuI	ACTGGG	2 cut(s)	634, 2694
BoxI	GACNNNNGTC	1 cut(s)	597
BpiI	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2781
BplI	GAGNNNNNCTC	2 cut(s)	589, 621
BpmI	CTGGAG	4 cut(s)	387, 616, 1054, 1455
Bpu10I	CCTNAGC	3 cut(s)	77, 843, 2369
Bpu1102I	GCTNAGC	2 cut(s)	225, 1236
Bpu14I	TTCGAA	2 cut(s)	887, 2714
BpuEI	CTTGAG	2 cut(s)	758, 1310
BpuMI	CCSGG	5 cut(s)	18, 69, 140, 1495, 1814
BsaAI	YACGTR	1 cut(s)	2531
BsaBI	GATNNNNATC	2 cut(s)	2302, 3084
BsaHI	GRCGYC	2 cut(s)	658, 1444
BsaI	GGTCTC	2 cut(s)	405, 1264
BsaJI	CCNNGG	7 cut(s)	716, 772, 1871, 2214, 2393, 2743, 3027
BsaWI	WCCGGW	3 cut(s)	89, 945, 1119
Bsc4I	CCNNNNNNNGG	8 cut(s)	93, 145, 296, 654, 1099, 1872, 1873, 2684
Bse118I	RCCGGY	4 cut(s)	89, 1119, 2491, 2911
Bse1I	ACTGG	6 cut(s)	423, 599, 629, 2568, 2689, 3098
Bse3DI	GCAATG	3 cut(s)	472, 2453, 2796
Bse8I	GATNNNNATC	2 cut(s)	2302, 3084
BseBI	CCWGG	7 cut(s)	291, 506, 1033, 2394, 2416, 2485, 3029
BseDI	CCNNGG	7 cut(s)	716, 772, 1871, 2214, 2393, 2743, 3027
BseGI	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2694
BseJI	GATNNNNATC	2 cut(s)	2302, 3084
BseLI	CCNNNNNNNGG	8 cut(s)	93, 145, 296, 654, 1099, 1872, 1873, 2684
BseMI	GCAATG	3 cut(s)	472, 2453, 2796
BseMII	CTCAG	9 cut(s)	179, 297, 857, 1323, 1352, 1535, 1794, 2447, 2561
BseNI	ACTGG	6 cut(s)	423, 599, 629, 2568, 2689, 3098
BseRI	GAGGAG	1 cut(s)	3013
BseXI	GCAGC	6 cut(s)	1238, 1357, 1360, 1870, 2092, 2927
BseYI	CCCAGC	1 cut(s)	1204
BsgI	GTGCAG	2 cut(s)	843, 2955
BshFI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BshNI	GGYRCC	1 cut(s)	1486
BshTI	ACCGGT	2 cut(s)	89, 1119
BsiHKAI	GWGCWC	1 cut(s)	2536
BslFI	GGGAC	3 cut(s)	1510, 2560, 2626
BslI	CCNNNNNNNGG	8 cut(s)	93, 145, 296, 654, 1099, 1872, 1873, 2684
BsmAI	GTCTC	6 cut(s)	405, 1264, 1328, 2065, 2204, 2603
BsmBI	CGTCTC	1 cut(s)	2065
BsmFI	GGGAC	3 cut(s)	1510, 2560, 2626
BsmI	GAATGC	3 cut(s)	721, 2336, 2911
BsnI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
Bso31I	GGTCTC	2 cut(s)	405, 1264
Bsp119I	TTCGAA	2 cut(s)	887, 2714
Bsp1286I	GDGCHC	4 cut(s)	327, 1040, 1522, 2536
Bsp1720I	GCTNAGC	2 cut(s)	225, 1236
BspACI	CCGC	3 cut(s)	8, 1244, 3054
BspANI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BspCNI	CTCAG	9 cut(s)	178, 298, 856, 1324, 1351, 1534, 1793, 2446, 2560
BspHI	TCATGA	1 cut(s)	277
BspLI	GGNNCC	9 cut(s)	301, 528, 538, 1037, 1488, 1666, 1713, 2164, 2733
BspMAI	CTGCAG	2 cut(s)	45, 379
BspMI	ACCTGC	2 cut(s)	2449, 3125
BspT104I	TTCGAA	2 cut(s)	887, 2714
BspT107I	GGYRCC	1 cut(s)	1486
BspTNI	GGTCTC	2 cut(s)	405, 1264
BsrBI	CCGCTC	1 cut(s)	3056
BsrDI	GCAATG	3 cut(s)	472, 2453, 2796
BsrFI	RCCGGY	4 cut(s)	89, 1119, 2491, 2911
BsrI	ACTGG	6 cut(s)	423, 599, 629, 2568, 2689, 3098
BssAI	RCCGGY	4 cut(s)	89, 1119, 2491, 2911
BssECI	CCNNGG	7 cut(s)	716, 772, 1871, 2214, 2393, 2743, 3027
BssNI	GRCGYC	2 cut(s)	658, 1444
BssT1I	CCWWGG	5 cut(s)	716, 772, 1871, 2214, 2743
Bst2UI	CCWGG	7 cut(s)	291, 506, 1033, 2394, 2416, 2485, 3029
Bst6I	CTCTTC	3 cut(s)	1145, 2348, 2938
BstACI	GRCGYC	2 cut(s)	658, 1444
BstAPI	GCANNNNNTGC	2 cut(s)	1232, 2258
BstBAI	YACGTR	1 cut(s)	2531
BstBI	TTCGAA	2 cut(s)	887, 2714
BstEII	GGTNACC	2 cut(s)	669, 1115
BstENI	CCTNNNNNAGG	1 cut(s)	652
BstF5I	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2694
BstMAI	GTCTC	6 cut(s)	405, 1264, 1328, 2065, 2204, 2603
BstNI	CCWGG	7 cut(s)	291, 506, 1033, 2394, 2416, 2485, 3029
BstNSI	RCATGY	1 cut(s)	1065
BstPAI	GACNNNNGTC	1 cut(s)	597
BstPI	GGTNACC	2 cut(s)	669, 1115
BstSFI	CTRYAG	2 cut(s)	41, 375
BstV1I	GCAGC	6 cut(s)	1238, 1357, 1360, 1870, 2092, 2927
BstV2I	GAAGAC	5 cut(s)	590, 896, 1545, 1845, 2781
BsuRI	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
BtrI	CACGTC	2 cut(s)	980, 2075
BtsCI	GGATG	5 cut(s)	671, 1078, 1273, 1576, 2694
BtsI	GCAGTG	3 cut(s)	322, 477, 1668
BveI	ACCTGC	2 cut(s)	2449, 3125
CaiI	CAGNNNCTG	3 cut(s)	254, 731, 1232
CciI	TCATGA	1 cut(s)	277
Cfr10I	RCCGGY	4 cut(s)	89, 1119, 2491, 2911
Cfr13I	GGNCC	5 cut(s)	943, 1418, 1665, 2353, 2675
CseI	GACGC	2 cut(s)	647, 1452
Csp6I	GTAC	2 cut(s)	2522, 2810
CspAI	ACCGGT	2 cut(s)	89, 1119
CspCI	CAANNNNNGTGG	2 cut(s)	292, 327
CviQI	GTAC	2 cut(s)	2522, 2810
DraI	TTTAAA	1 cut(s)	1386
DraIII	CACNNNGTG	3 cut(s)	109, 677, 2064
DriI	GACNNNNNGTC	1 cut(s)	618
EaeI	YGGCCR	4 cut(s)	136, 420, 1633, 2481
Eam1104I	CTCTTC	3 cut(s)	1145, 2348, 2938
Eam1105I	GACNNNNNGTC	1 cut(s)	618
EarI	CTCTTC	3 cut(s)	1145, 2348, 2938
Eco130I	CCWWGG	5 cut(s)	716, 772, 1871, 2214, 2743
Eco24I	GRGCYC	3 cut(s)	327, 1040, 1522
Eco31I	GGTCTC	2 cut(s)	405, 1264
Eco32I	GATATC	1 cut(s)	1980
Eco47I	GGWCC	3 cut(s)	943, 1418, 2675
Eco57I	CTGAAG	1 cut(s)	3087
Eco72I	CACGTG	1 cut(s)	2531
Eco91I	GGTNACC	2 cut(s)	669, 1115
EcoNI	CCTNNNNNAGG	1 cut(s)	652
EcoO65I	GGTNACC	2 cut(s)	669, 1115
EcoRI	GAATTC	1 cut(s)	2828
EcoRII	CCWGG	7 cut(s)	289, 504, 1031, 2392, 2414, 2483, 3027
EcoRV	GATATC	1 cut(s)	1980
EcoT14I	CCWWGG	5 cut(s)	716, 772, 1871, 2214, 2743
EcoT22I	ATGCAT	1 cut(s)	2338
EcoT38I	GRGCYC	3 cut(s)	327, 1040, 1522
ErhI	CCWWGG	5 cut(s)	716, 772, 1871, 2214, 2743
Esp3I	CGTCTC	1 cut(s)	2065
FalI	AAGNNNNNCTT	6 cut(s)	989, 1021, 2766, 2798, 3102, 3134
FaqI	GGGAC	3 cut(s)	1510, 2560, 2626
FbaI	TGATCA	4 cut(s)	190, 259, 1558, 3106
Fnu4HI	GCNGC	7 cut(s)	1227, 1245, 1371, 1374, 1884, 2106, 2916
FokI	GGATG	5 cut(s)	678, 1085, 1280, 1583, 2701
FriOI	GRGCYC	3 cut(s)	327, 1040, 1522
Fsp4HI	GCNGC	7 cut(s)	1227, 1245, 1371, 1374, 1884, 2106, 2916
FspBI	CTAG	5 cut(s)	501, 773, 1791, 2225, 3062
GluI	GCNGC	7 cut(s)	1227, 1245, 1371, 1374, 1884, 2106, 2916
GsaI	CCCAGC	1 cut(s)	1208
GsuI	CTGGAG	4 cut(s)	387, 616, 1054, 1455
HaeIII	GGCC	6 cut(s)	138, 422, 1635, 1667, 2355, 2483
HgaI	GACGC	2 cut(s)	647, 1452
Hin1I	GRCGYC	2 cut(s)	658, 1444
HincII	GTYRAC	1 cut(s)	208
HindII	GTYRAC	1 cut(s)	208
HindIII	AAGCTT	1 cut(s)	1326
HpaI	GTTAAC	1 cut(s)	208
HphI	GGTGA	8 cut(s)	663, 1109, 1114, 1178, 2298, 2518, 2886, 2956
Hpy166II	GTNNAC	5 cut(s)	208, 873, 2014, 2056, 3019
Hpy188I	TCNGA	9 cut(s)	168, 256, 559, 1333, 1558, 1972, 2436, 2848, 3085
Hpy8I	GTNNAC	5 cut(s)	208, 873, 2014, 2056, 3019
HpyAV	CCTTC	8 cut(s)	29, 550, 842, 1308, 1500, 1561, 1702, 1762
HpyCH4IV	ACGT	3 cut(s)	979, 2074, 2530
HpySE526I	ACGT	3 cut(s)	979, 2074, 2530
Hsp92I	GRCGYC	2 cut(s)	658, 1444
Ksp22I	TGATCA	4 cut(s)	190, 259, 1558, 3106
KspAI	GTTAAC	1 cut(s)	208
LmnI	GCTCC	6 cut(s)	542, 790, 1035, 2417, 2499, 3061
Lsp1109I	GCAGC	6 cut(s)	1238, 1357, 1360, 1870, 2092, 2927
LweI	GCATC	3 cut(s)	1120, 2228, 2248
MaeI	CTAG	5 cut(s)	501, 773, 1791, 2225, 3062
MaeII	ACGT	3 cut(s)	979, 2074, 2530
MbiI	CCGCTC	1 cut(s)	3056
MfeI	CAATTG	1 cut(s)	930
MhlI	GDGCHC	4 cut(s)	327, 1040, 1522, 2536
MlsI	TGGCCA	2 cut(s)	422, 2483
MluNI	TGGCCA	2 cut(s)	422, 2483
Mox20I	TGGCCA	2 cut(s)	422, 2483
Mph1103I	ATGCAT	1 cut(s)	2338
MroXI	GAANNNNTTC	1 cut(s)	2778
MscI	TGGCCA	2 cut(s)	422, 2483
MslI	CAYNNNNRTG	1 cut(s)	1745
Msp20I	TGGCCA	2 cut(s)	422, 2483
MspA1I	CMGCKG	1 cut(s)	2918
MunI	CAATTG	1 cut(s)	930
Mva1269I	GAATGC	3 cut(s)	721, 2336, 2911
MvaI	CCWGG	7 cut(s)	291, 506, 1033, 2394, 2416, 2485, 3029
NciI	CCSGG	5 cut(s)	18, 69, 140, 1495, 1814
NlaIV	GGNNCC	9 cut(s)	301, 528, 538, 1037, 1488, 1666, 1713, 2164, 2733
NsiI	ATGCAT	1 cut(s)	2338
NspI	RCATGY	1 cut(s)	1065
NspV	TTCGAA	2 cut(s)	887, 2714
PagI	TCATGA	1 cut(s)	277
PctI	GAATGC	3 cut(s)	721, 2336, 2911
PdmI	GAANNNNTTC	1 cut(s)	2778
PfeI	GAWTC	3 cut(s)	268, 705, 3080
PfoI	TCCNGGA	2 cut(s)	1031, 2414
PinAI	ACCGGT	2 cut(s)	89, 1119
PkrI	GCNGC	7 cut(s)	1228, 1246, 1372, 1375, 1885, 2107, 2917
PmaCI	CACGTG	1 cut(s)	2531
PmlI	CACGTG	1 cut(s)	2531
Ppu21I	YACGTR	1 cut(s)	2531
PshAI	GACNNNNGTC	1 cut(s)	597
PshBI	ATTAAT	1 cut(s)	2924
PsiI	TTATAA	2 cut(s)	353, 918
Psp6I	CCWGG	7 cut(s)	289, 504, 1031, 2392, 2414, 2483, 3027
PspCI	CACGTG	1 cut(s)	2531
PspEI	GGTNACC	2 cut(s)	669, 1115
PspFI	CCCAGC	1 cut(s)	1204
PspGI	CCWGG	7 cut(s)	289, 504, 1031, 2392, 2414, 2483, 3027
PspN4I	GGNNCC	9 cut(s)	301, 528, 538, 1037, 1488, 1666, 1713, 2164, 2733
PspPI	GGNCC	5 cut(s)	943, 1418, 1665, 2353, 2675
PstI	CTGCAG	2 cut(s)	45, 379
PstNI	CAGNNNCTG	3 cut(s)	254, 731, 1232
PvuII	CAGCTG	1 cut(s)	2918
RsaI	GTAC	2 cut(s)	2523, 2811
RsaNI	GTAC	2 cut(s)	2522, 2810
RseI	CAYNNNNRTG	1 cut(s)	1745
SatI	GCNGC	7 cut(s)	1227, 1245, 1371, 1374, 1884, 2106, 2916
Sau96I	GGNCC	5 cut(s)	943, 1418, 1665, 2353, 2675
SduI	GDGCHC	4 cut(s)	327, 1040, 1522, 2536
SfaNI	GCATC	3 cut(s)	1120, 2228, 2248
SfcI	CTRYAG	2 cut(s)	41, 375
SfuI	TTCGAA	2 cut(s)	887, 2714
SgrAI	CRCCGGYG	1 cut(s)	89
SinI	GGWCC	3 cut(s)	943, 1418, 2675
SmiMI	CAYNNNNRTG	1 cut(s)	1745
SmlI	CTYRAG	2 cut(s)	737, 1289
SmoI	CTYRAG	2 cut(s)	737, 1289
SsiI	CCGC	3 cut(s)	8, 1244, 3054
SspI	AATATT	2 cut(s)	2112, 2477
SspMI	CTAG	5 cut(s)	501, 773, 1791, 2225, 3062
StyI	CCWWGG	5 cut(s)	716, 772, 1871, 2214, 2743
TaiI	ACGT	3 cut(s)	982, 2077, 2533
TaqI	TCGA	7 cut(s)	413, 815, 887, 1397, 1821, 2346, 2714
TatI	WGTACW	2 cut(s)	2521, 2809
TauI	GCSGC	1 cut(s)	1247
TfiI	GAWTC	3 cut(s)	268, 705, 3080
TseI	GCWGC	6 cut(s)	1226, 1370, 1373, 1883, 2105, 2915
TspGWI	ACGGA	3 cut(s)	176, 2206, 2532
VpaK11BI	GGWCC	3 cut(s)	943, 1418, 2675
VspI	ATTAAT	1 cut(s)	2924
XagI	CCTNNNNNAGG	1 cut(s)	652
XapI	RAATTY	5 cut(s)	2401, 2419, 2828, 3049, 3074
XceI	RCATGY	1 cut(s)	1065
XmaJI	CCTAGG	1 cut(s)	772
XmnI	GAANNNNTTC	1 cut(s)	2778
XspI	CTAG	5 cut(s)	501, 773, 1791, 2225, 3062
Zsp2I	ATGCAT	1 cut(s)	2338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.