Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001666

Physical Location & Seq

Genomic Coordinates Forward (+)

5825638 .. 5833654

Gene Structure 8017 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1043400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGATTTCAGAGAAGCCCAGTTGGATTAGACATGAGGGAATGCAAATTTTCTCCATTGACGTTCAACCTGGTGGACTTAGGCTTGCCACTGGTGGGGGTGATCACAAGGTGCGGGTATGGAATATGAAATCCCTTGGCAGGGATTTGGATAATGAAGAATCAGCCCAGAGGCTACTTGCAACTCTCCGTGATCACTTTGGTTCTGTGAATTGTGTTAGGTGGGCTAAACATGGTCGGTTTCTTGCATCAGGATCTGATGACCAAGTGATTCTAATTCATGAGAGAAAGCCTGGTTCAGGAACCACTGAGTTTGGCAGTGGAGAGCCACCTGACCTTGAAAATTGGAAAGTTTCAATGACTTTGAGAGGGCATACTGCAGATGTGGTGGATCTTAATTGGTCTCCAGATGACTTGATGTTGGCCAGTGGAAGTTTAGATAACACTATACACATCTGGAACATGAGCAATGGTATTTGCACTGCTGTTCTGAGGGGTCACTCTAGCCTGGTTAAAGGAGTTACTTGGGATCCAATTGGCTCCTTCATAGCAAGTCAATCAGATGATAAGACTGTAATTATTTGGCGAACAAGTGACTGGAGCCTTGCTCACAGGACAGACGGCCATTGGCAAAAATCACTTGGATCCACCTTTTTCAGGCGGCTGGGATGGTCCCCTTGTGGCCACTTCATTACTACCACTCACGGATTCCAGAAACCAAGGCATTCTGCACCTGTTCTAGAGAGAGGGGAATGGTCTGCTACATTTGACTTCTTAGGACATAATGCCCCTGTGATCGTAGTGAAGTTTAACCATTCAATGTATAGGAGGAATATTTCCAATGCTCAGGAAAAAGCTGCACCCATCGGGTGGACTAATGGGGCTTCAAAGACAGGAGGAAAAGAGAAAGAACCACAACCTTATAATGTCATTGCTATTGGGAGTCAGGACAAGACTATAACAGTATGGACTACTGCAAGTCCCCGACCTCTCTTTGTAGCAAAGCATTTCTTTACTCAAAGTGTTGTGGATTTATCCTGGAGCCCTGATGGCTATTCACTTTTTGCATGTTCTTTGGATGGTTCGGTGGCAACTTTCCATTTTGATGTTAAAGAACTTGGCAACCGTTTAAGTGATGCTGAACTGGATGAATTGAAGAGAAACCGTTATGGAGATGTTAGAGGTCGCCAAGCAAATTTAGCAGAAAGCCCAGCTCAGTTATTGTTTGAAGCAGCATCTGCTAAGCAAGCCTCAAGCAAAAAAATGGTTCTGGAAGTTCAGCAAAACGAGACAGTTGGAAAACCTTCTACTGAAGCAACGGTTGCAACAATAACTTCTGCAGACAGCTTAAATAAGGTTTCAATAGCTGCCAGAATTTCAAGTCCTGTGAAGCAGAGGGAATATAGACGCCCTGATGGTAGAAAGAGGATTATTCCAGAAGCAGTTGGGGTGCCTCTGCAGCAGGAAAATATATCTGCTGGGGTTCAAACCCAGGCACTTGACTTCCCTTCTGAATGTTCTGATAAAAACAATGATGAGAATGGGTTAATTGCTGCTGATAGTGGCATCAAAGAAAGTTCTGTTAGGGGAGTAATTGGCAGAAGCACTGAAATAAAGGAAGGACATGGGGTTACTGCTAGAGCTATGATTACCAAGAGCCTTGTTATTGAGAAGGTTCCTGCTTCCACAGGTGGAGATGAAAGCATAGCTGTGGAACAGTCAGGTAATTTGAAGGCGTCTAGTTCAGCAGGTTCTTCATGTTCCACTCTTTCAATTAGGGTGTTTGATAGGAAAGAAGGGGAAGACAATGTACCAATATGCTTGGAAGCTCGACCTAGGGAACACGCTGCAAACGACATCGTTGGCTTGGGAAATACATTTATCATGAAAGAAACAGAAATTACTTGCGCAAGAGGGTTACAGACTCTTTGGTCAGATAGGATATCTGGGAAAGTCACTGTTTTAGCTGGAAATGCAAACTTCTGGGCTGTTGGGTGTGAAGATGGATGCATACAGGTTTACACAACTTGCGGGAGACGTGCTATGCCAACTATGATGGTAGGATCTGCAGCAATATTTATAGATTGTGACGAGCGCTGGAAATTATTTTTGGTCACAAGAAAAGGATCCTTTTATGTATGGGATCTATTCAAGCAGAATTGTCTCCTCCATGACTCATTGGCATCTCTGGTCGCTTCAAACCCAAACCCATCTGCAAAAGATGCAGGCGTGATCAAAGTTATATCAGCAAAGCTATCAAGATCTGGTTCTCCTCTTGTTGTTCTTGCCACTCGCCATGCCTTCCTCTTTGACATGGGCCTGATGTGTTGGCTTAGGGTTGCAGATGACTGCTTTCCTGGGTCAAATTTTGCAAGCTCCTGGCATTCAGGTTCAACTCCGGGTGGTGAGCTGGCTGCTTTGCAGATTGATGTTAGGAAGTATGTGGCCAGAAAGCCAGGTTGGAGCAGGGTGACAGACGATGGGGTGCAGACACGTGCTCACTTGGAGGCTCAGTTGGCTTCCTCGCTAGCTTTAAAGTCAGCCAAAGACTATTGCCAATGCCTTCTATCATATATTCGCTTCCTTGCAAGAGAAGCAGATGAGTCTCGTTTACGAGAAGTGTGTGAGAGTTTCCTTGGACCTCCAACTGGGATGGTGGACGATACAACTTTAGATTTAAACACCTGCTGCGGCATTGGGACTCTACTGTGTGCGTTGTTTAGAGTGTGGCTGTTCCATTCGATCAGGTGTCTTTGTCTTGTTGTCTTGTCATGTTGCGTTATTGTATTATTTTCTCTATCTCTTGCCATTAATCCGCCCCTGCTCAGTTCATTGAATACTATAGACTTTTTGATCACTGCTTTGCTGTTTTATGGCCTTTTGCCGACGGCGTTAAGGGTAGGAGCGGAACACGGTCACTATTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003682 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006336 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0030874 GO:0030875 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031491 GO:0031497 GO:0031935 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0034622 GO:0034724 GO:0034728 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044452 GO:0044454 GO:0044464 GO:0044877 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0080090 GO:0140110 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hira WD40

Protein Analysis

975

Amino Acids

106.42

Weight (kDa)

7.3

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	11 - 372	1.3e-55	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	12 - 98	7.3e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	15 - 197	1.4e-31	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	15 - 99	6.7e-09	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	18 - 201	2.3e-15	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	55 - 263	1.2e-20	CDC20/Fizzy WD40 domain
WD40	PF00400	57 - 92	1.7e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	66 - 271	3.4e-26	THOC3 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	68 - 230	6.2e-07	WDR35/TULP4 N-terminal
WD40_Gbeta	PF25391	110 - 193	9.1e-11	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	111 - 195	7.5e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	112 - 211	2.8e-17	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	112 - 198	2.3e-13	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	112 - 211	4.1e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	115 - 211	7.1e-09	WDR3 second beta-propeller domain
WD40	PF00400	116 - 153	2.4e-09	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	119 - 258	1.4e-14	WDHD1 first WD40 domain
Beta-prop_Aladin	PF25460	123 - 231	5.2e-08	Aladin seven-bladed propeller
WD40	PF00400	158 - 194	2.5e-06	WD domain, G-beta repeat
Hira	PF07569	677 - 876	6.1e-48	TUP1-like enhancer of split

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44530 AT3G44530 AT3G44530 AT3G44530
fragaria_vesca	FvH4_3g24580 FvH4_3g24580 FvH4_3g24580 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610 FvH4_7g01610
malus_domestica	MD00G1078300.v1.1 MD10G1043400.v1.1
prunus_persica	Prupe.8G049500_v2.0.a1 Prupe.8G049500_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1 Prupe.8G049600_v2.0.a1
pyrus_communis	pycom05g03110 pycom10g03040
rosa_chinensis	RchiOBHm_Chr1g0317731 RchiOBHm_Chr5g0044121
rosa_laevigata	RLG00000030587 RLG00000034243 RLG00000034244
rosa_multiflora	Rmu_sc0000939.1_g000028 Rmu_sc0001158.1_g000004
rosa_roxburghii	Rroxscaffold_1G00036920 Rroxscaffold_4G00330210
rosa_rugosa	Rorug01G0016900.1 Rorug05G0210500 Rorug05G0210600
rosa_samantha	Rh1AG029200 Rh1BG025400 Rh1CG026500 Rh1DG044000 Rh5AG294700 Rh5BG303400 Rh5CG330300 Rh5DG312200
rosa_wichuraiana	Rw0G013170 Rw0G019570 Rw5G027330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	921
AarI	CACCTGC	1 cut(s)	2690
AasI	GACNNNNNNGTC	1 cut(s)	1927
Acc16I	TGCGCA	1 cut(s)	1906
Acc36I	ACCTGC	2 cut(s)	1736, 2690
AccB1I	GGYRCC	1 cut(s)	1447
AccB7I	CCANNNNNTGG	1 cut(s)	867
AccBSI	CCGCTC	1 cut(s)	2903
AciI	CCGC	6 cut(s)	112, 658, 2028, 2688, 2813, 2903
AcoI	YGGCCR	4 cut(s)	420, 619, 679, 2442
AcsI	RAATTY	4 cut(s)	45, 1192, 1371, 2362
AcuI	CTGAAG	1 cut(s)	1329
AcvI	CACGTG	1 cut(s)	2492
AcyI	GRCGYC	2 cut(s)	1405, 1733
AdeI	CACNNNGTG	1 cut(s)	109
AfaI	GTAC	1 cut(s)	1809
AfeI	AGCGCT	1 cut(s)	2093
AfiI	CCNNNNNNNGG	7 cut(s)	93, 138, 139, 296, 654, 867, 2645
AflIII	ACRYGT	1 cut(s)	2489
AjiI	CACGTC	1 cut(s)	2036
AjnI	CCWGG	8 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2452
AjuI	GAANNNNNNNTTGG	4 cut(s)	2090, 2122, 2440, 2472
Alw21I	GWGCWC	1 cut(s)	2497
Alw26I	GTCTC	5 cut(s)	405, 1280, 2026, 2165, 2607
AlwNI	CAGNNNCTG	2 cut(s)	254, 1235
Aor51HI	AGCGCT	1 cut(s)	2093
AoxI	GGCC	6 cut(s)	420, 619, 679, 2314, 2442, 2872
ApeKI	GCWGC	9 cut(s)	854, 1229, 1364, 1456, 1550, 1844, 2066, 2411, 2685
ApoI	RAATTY	4 cut(s)	45, 1192, 1371, 2362
AseI	ATTAAT	1 cut(s)	2808
Asp700I	GAANNNNTTC	1 cut(s)	1300
AspA2I	CCTAGG	1 cut(s)	1832
AspLEI	GCGC	2 cut(s)	1907, 2094
AspS9I	GGNCC	3 cut(s)	669, 2314, 2636
AsuC2I	CCSGG	1 cut(s)	2397
AsuHPI	GGTGA	3 cut(s)	110, 2414, 2479
AsuNHI	GCTAGC	1 cut(s)	2524
AvaII	GGWCC	2 cut(s)	669, 2636
AvrII	CCTAGG	1 cut(s)	1832
BalI	TGGCCA	3 cut(s)	422, 681, 2444
BamHI	GGATCC	3 cut(s)	526, 641, 2123
BanI	GGYRCC	1 cut(s)	1447
BanII	GRGCYC	1 cut(s)	1043
BarI	GAAGNNNNNNTAC	2 cut(s)	1791, 1823
BbrPI	CACGTG	1 cut(s)	2492
BbsI	GAAGAC	1 cut(s)	1806
Bbv12I	GWGCWC	1 cut(s)	2497
BbvI	GCAGC	9 cut(s)	841, 1241, 1351, 1468, 1537, 1831, 2078, 2398, 2672
BceAI	ACGGC	2 cut(s)	634, 2901
BciT130I	CCWGG	8 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2454
BclI	TGATCA	4 cut(s)	100, 190, 2229, 2850
BcnI	CCSGG	1 cut(s)	2397
BcoDI	GTCTC	5 cut(s)	405, 1280, 2026, 2165, 2607
BfaI	CTAG	6 cut(s)	501, 737, 1635, 1737, 1833, 2525
BfmI	CTRYAG	5 cut(s)	375, 1335, 1454, 2064, 2838
BfoI	RGCGCY	1 cut(s)	2095
BfuAI	ACCTGC	2 cut(s)	1736, 2690
BglI	GCCNNNNNGGC	1 cut(s)	1047
BglII	AGATCT	1 cut(s)	2258
BlnI	CCTAGG	1 cut(s)	1832
BlpI	GCTNAGC	1 cut(s)	1239
Bme1390I	CCNGG	9 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2397, 2454
Bme18I	GGWCC	2 cut(s)	669, 2636
BmgBI	CACGTC	1 cut(s)	2036
BmgT120I	GGNCC	3 cut(s)	669, 2314, 2636
BmrFI	CCNGG	9 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2397, 2454
BmrI	ACTGGG	2 cut(s)	12, 2655
BmsI	GCATC	7 cut(s)	254, 1123, 1241, 1572, 1994, 2189, 2209
BmtI	GCTAGC	1 cut(s)	2528
BmuI	ACTGGG	2 cut(s)	12, 2655
BpiI	GAAGAC	1 cut(s)	1806
BplI	GAGNNNNNCTC	2 cut(s)	589, 621
BpmI	CTGGAG	3 cut(s)	387, 616, 1057
Bpu10I	CCTNAGC	2 cut(s)	843, 2330
Bpu1102I	GCTNAGC	1 cut(s)	1239
BpuEI	CTTGAG	1 cut(s)	1234
BpuMI	CCSGG	1 cut(s)	2397
BsaAI	YACGTR	1 cut(s)	2492
BsaHI	GRCGYC	2 cut(s)	1405, 1733
BsaI	GGTCTC	1 cut(s)	405
BsaJI	CCNNGG	6 cut(s)	133, 716, 1488, 1832, 2354, 2632
BsaXI	ACNNNNNCTCC	2 cut(s)	2495, 2525
Bsc4I	CCNNNNNNNGG	7 cut(s)	93, 138, 139, 296, 654, 867, 2645
Bse1I	ACTGG	6 cut(s)	18, 94, 423, 599, 1146, 2650
Bse3DI	GCAATG	2 cut(s)	472, 927
BseBI	CCWGG	8 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2454
BseDI	CCNNGG	6 cut(s)	133, 716, 1488, 1832, 2354, 2632
BseGI	GGATG	5 cut(s)	671, 1081, 1150, 2009, 2655
BseLI	CCNNNNNNNGG	7 cut(s)	93, 138, 139, 296, 654, 867, 2645
BseMI	GCAATG	2 cut(s)	472, 927
BseMII	CTCAG	6 cut(s)	297, 479, 857, 1226, 2522, 2836
BseNI	ACTGG	6 cut(s)	18, 94, 423, 599, 1146, 2650
BseRI	GAGGAG	2 cut(s)	2153, 2259
BseXI	GCAGC	9 cut(s)	841, 1241, 1351, 1468, 1537, 1831, 2078, 2398, 2672
BseYI	CCCAGC	3 cut(s)	661, 1207, 1475
BsgI	GTGCAG	3 cut(s)	711, 840, 2504
BshFI	GGCC	6 cut(s)	422, 621, 681, 2316, 2444, 2874
BshNI	GGYRCC	1 cut(s)	1447
BsiHKAI	GWGCWC	1 cut(s)	2497
BsiSI	CCGG	1 cut(s)	2396
BslFI	GGGAC	3 cut(s)	655, 963, 2710
BslI	CCNNNNNNNGG	7 cut(s)	93, 138, 139, 296, 654, 867, 2645
BsmAI	GTCTC	5 cut(s)	405, 1280, 2026, 2165, 2607
BsmBI	CGTCTC	1 cut(s)	2026
BsmFI	GGGAC	3 cut(s)	655, 963, 2710
BsmI	GAATGC	3 cut(s)	45, 721, 2380
BsnI	GGCC	6 cut(s)	422, 621, 681, 2316, 2444, 2874
Bso31I	GGTCTC	1 cut(s)	405
Bsp1286I	GDGCHC	2 cut(s)	1043, 2497
Bsp1720I	GCTNAGC	1 cut(s)	1239
BspACI	CCGC	6 cut(s)	112, 658, 2028, 2688, 2813, 2903
BspANI	GGCC	6 cut(s)	422, 621, 681, 2316, 2444, 2874
BspCNI	CTCAG	6 cut(s)	298, 480, 856, 1225, 2521, 2835
BspHI	TCATGA	2 cut(s)	277, 1881
BspMAI	CTGCAG	4 cut(s)	379, 1339, 1458, 2068
BspMI	ACCTGC	2 cut(s)	1736, 2690
BspOI	GCTAGC	1 cut(s)	2528
BspT107I	GGYRCC	1 cut(s)	1447
BspTNI	GGTCTC	1 cut(s)	405
BsrBI	CCGCTC	1 cut(s)	2903
BsrDI	GCAATG	2 cut(s)	472, 927
BsrI	ACTGG	6 cut(s)	18, 94, 423, 599, 1146, 2650
BssECI	CCNNGG	6 cut(s)	133, 716, 1488, 1832, 2354, 2632
BssNI	GRCGYC	2 cut(s)	1405, 1733
BssT1I	CCWWGG	4 cut(s)	133, 716, 1832, 2632
Bst2UI	CCWGG	8 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2454
Bst6I	CTCTTC	1 cut(s)	1148
BstACI	GRCGYC	2 cut(s)	1405, 1733
BstAPI	GCANNNNNTGC	3 cut(s)	1235, 1319, 2219
BstBAI	YACGTR	1 cut(s)	2492
BstC8I	GCNNGC	6 cut(s)	84, 1245, 2224, 2371, 2409, 2526
BstENI	CCTNNNNNAGG	2 cut(s)	294, 652
BstF5I	GGATG	5 cut(s)	671, 1081, 1150, 2009, 2655
BstH2I	RGCGCY	1 cut(s)	2095
BstHHI	GCGC	2 cut(s)	1907, 2094
BstMAI	GTCTC	5 cut(s)	405, 1280, 2026, 2165, 2607
BstNI	CCWGG	8 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2454
BstNSI	RCATGY	1 cut(s)	1068
BstSCI	CCNGG	9 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2395, 2452
BstSFI	CTRYAG	5 cut(s)	375, 1335, 1454, 2064, 2838
BstV1I	GCAGC	9 cut(s)	841, 1241, 1351, 1468, 1537, 1831, 2078, 2398, 2672
BstV2I	GAAGAC	1 cut(s)	1806
BstX2I	RGATCY	8 cut(s)	251, 388, 526, 641, 2060, 2123, 2140, 2258
BstYI	RGATCY	8 cut(s)	251, 388, 526, 641, 2060, 2123, 2140, 2258
BsuRI	GGCC	6 cut(s)	422, 621, 681, 2316, 2444, 2874
BtrI	CACGTC	1 cut(s)	2036
BtsCI	GGATG	5 cut(s)	671, 1081, 1150, 2009, 2655
BtsI	GCAGTG	3 cut(s)	322, 477, 2853
BtsIMutI	CAGTG	8 cut(s)	87, 303, 322, 430, 477, 1602, 1953, 2853
BveI	ACCTGC	2 cut(s)	1736, 2690
Cac8I	GCNNGC	6 cut(s)	84, 1245, 2224, 2371, 2409, 2526
CaiI	CAGNNNCTG	2 cut(s)	254, 1235
CciI	TCATGA	2 cut(s)	277, 1881
CfoI	GCGC	2 cut(s)	1907, 2094
Cfr13I	GGNCC	3 cut(s)	669, 2314, 2636
CseI	GACGC	2 cut(s)	1413, 1722
CsiI	ACCWGGT	1 cut(s)	67
Csp6I	GTAC	1 cut(s)	1808
CspCI	CAANNNNNGTGG	2 cut(s)	292, 327
CviQI	GTAC	1 cut(s)	1808
DraI	TTTAAA	2 cut(s)	2532, 2676
DraIII	CACNNNGTG	1 cut(s)	109
DrdI	GACNNNNNNGTC	1 cut(s)	1927
DseDI	GACNNNNNNGTC	1 cut(s)	1927
EaeI	YGGCCR	4 cut(s)	420, 619, 679, 2442
Eam1104I	CTCTTC	1 cut(s)	1148
EarI	CTCTTC	1 cut(s)	1148
EciI	GGCGGA	1 cut(s)	2802
Eco130I	CCWWGG	4 cut(s)	133, 716, 1832, 2632
Eco24I	GRGCYC	1 cut(s)	1043
Eco31I	GGTCTC	1 cut(s)	405
Eco32I	GATATC	1 cut(s)	1941
Eco47I	GGWCC	2 cut(s)	669, 2636
Eco47III	AGCGCT	1 cut(s)	2093
Eco57I	CTGAAG	1 cut(s)	1329
Eco72I	CACGTG	1 cut(s)	2492
EcoNI	CCTNNNNNAGG	2 cut(s)	294, 652
EcoRII	CCWGG	8 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2452
EcoRV	GATATC	1 cut(s)	1941
EcoT14I	CCWWGG	4 cut(s)	133, 716, 1832, 2632
EcoT22I	ATGCAT	1 cut(s)	2009
EcoT38I	GRGCYC	1 cut(s)	1043
ErhI	CCWWGG	4 cut(s)	133, 716, 1832, 2632
Esp3I	CGTCTC	1 cut(s)	2026
FalI	AAGNNNNNCTT	2 cut(s)	992, 1024
FaqI	GGGAC	3 cut(s)	655, 963, 2710
FauI	CCCGC	2 cut(s)	105, 2021
FbaI	TGATCA	4 cut(s)	100, 190, 2229, 2850
FokI	GGATG	5 cut(s)	678, 1088, 1157, 2016, 2662
FriOI	GRGCYC	1 cut(s)	1043
FspBI	CTAG	6 cut(s)	501, 737, 1635, 1737, 1833, 2525
FspI	TGCGCA	1 cut(s)	1906
GlaI	GCGC	2 cut(s)	1906, 2093
GsaI	CCCAGC	3 cut(s)	665, 1211, 1479
GsuI	CTGGAG	3 cut(s)	387, 616, 1057
HaeII	RGCGCY	1 cut(s)	2095
HaeIII	GGCC	6 cut(s)	422, 621, 681, 2316, 2444, 2874
HapII	CCGG	1 cut(s)	2396
HgaI	GACGC	2 cut(s)	1413, 1722
HhaI	GCGC	2 cut(s)	1907, 2094
Hin1I	GRCGYC	2 cut(s)	1405, 1733
Hin6I	GCGC	2 cut(s)	1905, 2092
HinP1I	GCGC	2 cut(s)	1905, 2092
HinfI	GANTC	8 cut(s)	158, 268, 705, 940, 1921, 2171, 2600, 2698
HpaII	CCGG	1 cut(s)	2396
HphI	GGTGA	3 cut(s)	110, 2414, 2479
Hpy166II	GTNNAC	5 cut(s)	74, 870, 2017, 2609, 2656
Hpy188I	TCNGA	7 cut(s)	10, 256, 489, 559, 1510, 1519, 1933
Hpy8I	GTNNAC	5 cut(s)	74, 870, 2017, 2609, 2656
Hpy99I	CGWCG	1 cut(s)	2887
HpyAV	CCTTC	9 cut(s)	550, 1311, 1515, 1610, 1663, 1723, 1787, 2308, 2570
HpyCH4IV	ACGT	3 cut(s)	60, 2035, 2491
HpySE526I	ACGT	3 cut(s)	60, 2035, 2491
Hsp92I	GRCGYC	2 cut(s)	1405, 1733
HspAI	GCGC	2 cut(s)	1905, 2092
Ksp22I	TGATCA	4 cut(s)	100, 190, 2229, 2850
LmnI	GCTCC	6 cut(s)	542, 597, 1038, 2378, 2460, 2900
Lsp1109I	GCAGC	9 cut(s)	841, 1241, 1351, 1468, 1537, 1831, 2078, 2398, 2672
LweI	GCATC	7 cut(s)	254, 1123, 1241, 1572, 1994, 2189, 2209
MabI	ACCWGGT	1 cut(s)	67
MaeI	CTAG	6 cut(s)	501, 737, 1635, 1737, 1833, 2525
MaeII	ACGT	3 cut(s)	60, 2035, 2491
MbiI	CCGCTC	1 cut(s)	2903
MboII	GAAGA	5 cut(s)	167, 1165, 1743, 1811, 2009
MfeI	CAATTG	1 cut(s)	531
MflI	RGATCY	8 cut(s)	251, 388, 526, 641, 2060, 2123, 2140, 2258
MhlI	GDGCHC	2 cut(s)	1043, 2497
MlsI	TGGCCA	3 cut(s)	422, 681, 2444
MluNI	TGGCCA	3 cut(s)	422, 681, 2444
MlyI	GAGTC	5 cut(s)	949, 1915, 2165, 2609, 2692
MmeI	TCCRAC	3 cut(s)	1273, 2438, 2666
Mox20I	TGGCCA	3 cut(s)	422, 681, 2444
Mph1103I	ATGCAT	1 cut(s)	2009
MroXI	GAANNNNTTC	1 cut(s)	1300
MscI	TGGCCA	3 cut(s)	422, 681, 2444
MslI	CAYNNNNRTG	2 cut(s)	1101, 1706
Msp20I	TGGCCA	3 cut(s)	422, 681, 2444
MspI	CCGG	1 cut(s)	2396
MspR9I	CCNGG	9 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2397, 2454
MunI	CAATTG	1 cut(s)	531
Mva1269I	GAATGC	3 cut(s)	45, 721, 2380
MvaI	CCWGG	8 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2454
NciI	CCSGG	1 cut(s)	2397
NheI	GCTAGC	1 cut(s)	2524
NmuCI	GTSAC	7 cut(s)	494, 590, 1951, 2084, 2110, 2467, 2912
NsbI	TGCGCA	1 cut(s)	1906
NsiI	ATGCAT	1 cut(s)	2009
NspI	RCATGY	1 cut(s)	1068
PagI	TCATGA	2 cut(s)	277, 1881
PaqCI	CACCTGC	1 cut(s)	2690
PcsI	WCGNNNNNNNCGW	1 cut(s)	1848
PctI	GAATGC	3 cut(s)	45, 721, 2380
PdmI	GAANNNNTTC	1 cut(s)	1300
PfeI	GAWTC	3 cut(s)	158, 268, 705
PflMI	CCANNNNNTGG	1 cut(s)	867
PfoI	TCCNGGA	1 cut(s)	1034
PleI	GAGTC	5 cut(s)	948, 1915, 2165, 2608, 2692
PmaCI	CACGTG	1 cut(s)	2492
PmlI	CACGTG	1 cut(s)	2492
PpsI	GAGTC	5 cut(s)	948, 1915, 2165, 2608, 2692
Ppu21I	YACGTR	1 cut(s)	2492
PshBI	ATTAAT	1 cut(s)	2808
PsiI	TTATAA	1 cut(s)	921
Psp6I	CCWGG	8 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2452
PspCI	CACGTG	1 cut(s)	2492
PspFI	CCCAGC	3 cut(s)	661, 1207, 1475
PspGI	CCWGG	8 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2452
PspPI	GGNCC	3 cut(s)	669, 2314, 2636
PstI	CTGCAG	4 cut(s)	379, 1339, 1458, 2068
PstNI	CAGNNNCTG	2 cut(s)	254, 1235
PsuI	RGATCY	8 cut(s)	251, 388, 526, 641, 2060, 2123, 2140, 2258
RsaI	GTAC	1 cut(s)	1809
RsaNI	GTAC	1 cut(s)	1808
RseI	CAYNNNNRTG	2 cut(s)	1101, 1706
Sau96I	GGNCC	3 cut(s)	669, 2314, 2636
SchI	GAGTC	5 cut(s)	949, 1915, 2165, 2609, 2692
ScrFI	CCNGG	9 cut(s)	69, 291, 506, 1036, 1490, 2355, 2377, 2397, 2454
SduI	GDGCHC	2 cut(s)	1043, 2497
SexAI	ACCWGGT	1 cut(s)	67
SfaNI	GCATC	7 cut(s)	254, 1123, 1241, 1572, 1994, 2189, 2209
SfcI	CTRYAG	5 cut(s)	375, 1335, 1454, 2064, 2838
SinI	GGWCC	2 cut(s)	669, 2636
SmiMI	CAYNNNNRTG	2 cut(s)	1101, 1706
SmlI	CTYRAG	1 cut(s)	1249
SmoI	CTYRAG	1 cut(s)	1249
SsiI	CCGC	6 cut(s)	112, 658, 2028, 2688, 2813, 2903
SspI	AATATT	2 cut(s)	832, 2073
SspMI	CTAG	6 cut(s)	501, 737, 1635, 1737, 1833, 2525
StyD4I	CCNGG	9 cut(s)	67, 289, 504, 1034, 1488, 2353, 2375, 2395, 2452
StyI	CCWWGG	4 cut(s)	133, 716, 1832, 2632
TaiI	ACGT	3 cut(s)	63, 2038, 2494
TaqI	TCGA	2 cut(s)	1828, 2738
TauI	GCSGC	2 cut(s)	661, 2691
TfiI	GAWTC	3 cut(s)	158, 268, 705
TscAI	CASTG	8 cut(s)	94, 310, 322, 430, 484, 1609, 1960, 2860
TseFI	GTSAC	7 cut(s)	494, 590, 1951, 2084, 2110, 2467, 2912
TseI	GCWGC	9 cut(s)	854, 1229, 1364, 1456, 1550, 1844, 2066, 2411, 2685
Tsp45I	GTSAC	7 cut(s)	494, 590, 1951, 2084, 2110, 2467, 2912
TspGWI	ACGGA	2 cut(s)	176, 717
TspRI	CASTG	8 cut(s)	94, 310, 322, 430, 484, 1609, 1960, 2860
Van91I	CCANNNNNTGG	1 cut(s)	867
VpaK11BI	GGWCC	2 cut(s)	669, 2636
VspI	ATTAAT	1 cut(s)	2808
XagI	CCTNNNNNAGG	2 cut(s)	294, 652
XapI	RAATTY	4 cut(s)	45, 1192, 1371, 2362
XbaI	TCTAGA	1 cut(s)	736
XceI	RCATGY	1 cut(s)	1068
XmaJI	CCTAGG	1 cut(s)	1832
XmnI	GAANNNNTTC	1 cut(s)	1300
XspI	CTAG	6 cut(s)	501, 737, 1635, 1737, 1833, 2525
Zsp2I	ATGCAT	1 cut(s)	2009

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.