Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage (By similarity)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G45577 AT3G45590 AT3G45590 AT5G60230 AT5G60230 |
| fragaria_vesca | FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 |
| rosa_chinensis | RchiOBHm_Chr6g0276201 |
| rosa_laevigata | RLG00000013409 RLG00000025847 |
| rosa_multiflora | Rmu_sc0002063.1_g000007 Rmu_sc0004048.1_g000016 Rmu_sc0008839.1_g000006 |
| rosa_roxburghii | Rroxscaffold_2G00125430 Rroxscaffold_7G00192490 |
| rosa_rugosa | Rorug06G0098800 Rorug06G0098900 Rorug06G0098900 Rorug06G0099000 Rorug06G0099100 Rorug06G0099200 Rorug06G0099300 Rorug06G0124300 |
| rosa_samantha | Rh1AG171200 Rh3BG017000 Rh3DG017300 Rh5BG538000 Rh5CG561900 Rh6AG210700 Rh6BG214900 Rh6DG207200 |
| rosa_wichuraiana | Rw6G018360 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AccII | CGCG | 1 cut(s) | 566 |
| AciI | CCGC | 1 cut(s) | 501 |
| AclWI | GGATC | 2 cut(s) | 177, 321 |
| AfaI | GTAC | 2 cut(s) | 132, 365 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 100, 501 |
| AgsI | TTSAA | 2 cut(s) | 239, 653 |
| AhlI | ACTAGT | 1 cut(s) | 190 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 560, 592 |
| AleI | CACNNNNGTG | 1 cut(s) | 10 |
| AluBI | AGCT | 4 cut(s) | 76, 158, 163, 245 |
| AluI | AGCT | 4 cut(s) | 76, 158, 163, 245 |
| Alw26I | GTCTC | 1 cut(s) | 73 |
| AlwI | GGATC | 2 cut(s) | 177, 321 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 53 |
| AspS9I | GGNCC | 1 cut(s) | 525 |
| AsuII | TTCGAA | 1 cut(s) | 59 |
| AvaII | GGWCC | 1 cut(s) | 525 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BbvCI | CCTCAGC | 1 cut(s) | 30 |
| BccI | CCATC | 3 cut(s) | 213, 463, 666 |
| BceAI | ACGGC | 2 cut(s) | 464, 675 |
| BcoDI | GTCTC | 1 cut(s) | 73 |
| BcuI | ACTAGT | 1 cut(s) | 190 |
| BfaI | CTAG | 1 cut(s) | 191 |
| BglII | AGATCT | 1 cut(s) | 80 |
| BlpI | GCTNAGC | 1 cut(s) | 159 |
| Bme18I | GGWCC | 1 cut(s) | 525 |
| BmgT120I | GGNCC | 1 cut(s) | 525 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| BmsI | GCATC | 2 cut(s) | 282, 388 |
| Bpu10I | CCTNAGC | 1 cut(s) | 30 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 159 |
| Bpu14I | TTCGAA | 1 cut(s) | 59 |
| BpuEI | CTTGAG | 1 cut(s) | 103 |
| BsaAI | YACGTR | 1 cut(s) | 668 |
| BsaJI | CCNNGG | 2 cut(s) | 8, 99 |
| BsaWI | WCCGGW | 1 cut(s) | 554 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 100, 501 |
| Bse1I | ACTGG | 1 cut(s) | 401 |
| Bse3DI | GCAATG | 1 cut(s) | 535 |
| BseDI | CCNNGG | 2 cut(s) | 8, 99 |
| BseGI | GGATG | 1 cut(s) | 451 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 100, 501 |
| BseMI | GCAATG | 1 cut(s) | 535 |
| BseMII | CTCAG | 4 cut(s) | 21, 68, 150, 195 |
| BseNI | ACTGG | 1 cut(s) | 401 |
| Bsh1236I | CGCG | 1 cut(s) | 566 |
| Bsh1285I | CGRYCG | 2 cut(s) | 295, 389 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BsiEI | CGRYCG | 2 cut(s) | 295, 389 |
| BsiSI | CCGG | 1 cut(s) | 555 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 100, 501 |
| BsmAI | GTCTC | 1 cut(s) | 73 |
| Bsp119I | TTCGAA | 1 cut(s) | 59 |
| Bsp1407I | TGTACA | 1 cut(s) | 363 |
| Bsp143I | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 159 |
| Bsp68I | TCGCGA | 1 cut(s) | 566 |
| BspACI | CCGC | 1 cut(s) | 501 |
| BspCNI | CTCAG | 4 cut(s) | 22, 69, 151, 196 |
| BspFNI | CGCG | 1 cut(s) | 566 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspPI | GGATC | 2 cut(s) | 177, 321 |
| BspQI | GCTCTTC | 1 cut(s) | 648 |
| BspT104I | TTCGAA | 1 cut(s) | 59 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BsrDI | GCAATG | 1 cut(s) | 535 |
| BsrGI | TGTACA | 1 cut(s) | 363 |
| BsrI | ACTGG | 1 cut(s) | 401 |
| BssECI | CCNNGG | 2 cut(s) | 8, 99 |
| BssMI | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| BssT1I | CCWWGG | 1 cut(s) | 8 |
| Bst4CI | ACNGT | 3 cut(s) | 67, 293, 649 |
| Bst6I | CTCTTC | 1 cut(s) | 648 |
| BstAUI | TGTACA | 1 cut(s) | 363 |
| BstBAI | YACGTR | 1 cut(s) | 668 |
| BstBI | TTCGAA | 1 cut(s) | 59 |
| BstDEI | CTNAG | 5 cut(s) | 30, 77, 159, 204, 686 |
| BstF5I | GGATG | 1 cut(s) | 451 |
| BstFNI | CGCG | 1 cut(s) | 566 |
| BstKTI | GATC | 7 cut(s) | 83, 172, 329, 360, 389, 410, 511 |
| BstMAI | GTCTC | 1 cut(s) | 73 |
| BstMBI | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| BstMCI | CGRYCG | 2 cut(s) | 295, 389 |
| BstSNI | TACGTA | 1 cut(s) | 668 |
| BstUI | CGCG | 1 cut(s) | 566 |
| BstX2I | RGATCY | 1 cut(s) | 80 |
| BstYI | RGATCY | 1 cut(s) | 80 |
| BtsCI | GGATG | 1 cut(s) | 451 |
| BtsI | GCAGTG | 1 cut(s) | 547 |
| BtsIMutI | CAGTG | 1 cut(s) | 547 |
| BtuMI | TCGCGA | 1 cut(s) | 566 |
| CaiI | CAGNNNCTG | 1 cut(s) | 53 |
| Cfr13I | GGNCC | 1 cut(s) | 525 |
| CseI | GACGC | 1 cut(s) | 550 |
| Csp6I | GTAC | 2 cut(s) | 131, 364 |
| CviAII | CATG | 2 cut(s) | 331, 711 |
| CviJI | RGCY | 7 cut(s) | 76, 104, 158, 163, 245, 371, 690 |
| CviKI_1 | RGCY | 7 cut(s) | 76, 104, 158, 163, 245, 371, 690 |
| CviQI | GTAC | 2 cut(s) | 131, 364 |
| DdeI | CTNAG | 5 cut(s) | 30, 77, 159, 204, 686 |
| DpnI | GATC | 7 cut(s) | 82, 171, 328, 359, 388, 409, 510 |
| DpnII | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| Eam1104I | CTCTTC | 1 cut(s) | 648 |
| EarI | CTCTTC | 1 cut(s) | 648 |
| Eco105I | TACGTA | 1 cut(s) | 668 |
| Eco130I | CCWWGG | 1 cut(s) | 8 |
| Eco32I | GATATC | 1 cut(s) | 532 |
| Eco47I | GGWCC | 1 cut(s) | 525 |
| EcoRV | GATATC | 1 cut(s) | 532 |
| EcoT14I | CCWWGG | 1 cut(s) | 8 |
| EcoT22I | ATGCAT | 1 cut(s) | 275 |
| ErhI | CCWWGG | 1 cut(s) | 8 |
| FaeI | CATG | 2 cut(s) | 334, 714 |
| FaiI | YATR | 5 cut(s) | 322, 332, 423, 444, 712 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 147, 179 |
| FatI | CATG | 2 cut(s) | 330, 710 |
| FauI | CCCGC | 1 cut(s) | 494 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| FokI | GGATG | 1 cut(s) | 438 |
| FspBI | CTAG | 1 cut(s) | 191 |
| HapII | CCGG | 1 cut(s) | 555 |
| HgaI | GACGC | 1 cut(s) | 550 |
| Hin1II | CATG | 2 cut(s) | 334, 714 |
| HincII | GTYRAC | 2 cut(s) | 592, 604 |
| HindII | GTYRAC | 2 cut(s) | 592, 604 |
| HindIII | AAGCTT | 1 cut(s) | 243 |
| HinfI | GANTC | 4 cut(s) | 146, 223, 619, 676 |
| HpaII | CCGG | 1 cut(s) | 555 |
| Hpy166II | GTNNAC | 4 cut(s) | 188, 466, 592, 604 |
| Hpy188I | TCNGA | 4 cut(s) | 73, 151, 472, 529 |
| Hpy188III | TCNNGA | 4 cut(s) | 277, 302, 565, 680 |
| Hpy8I | GTNNAC | 4 cut(s) | 188, 466, 592, 604 |
| Hpy99I | CGWCG | 1 cut(s) | 566 |
| HpyAV | CCTTC | 1 cut(s) | 296 |
| HpyCH4III | ACNGT | 3 cut(s) | 67, 293, 649 |
| HpyCH4IV | ACGT | 3 cut(s) | 129, 588, 667 |
| HpyCH4V | TGCA | 3 cut(s) | 47, 273, 540 |
| HpyF3I | CTNAG | 5 cut(s) | 30, 77, 159, 204, 686 |
| HpySE526I | ACGT | 3 cut(s) | 129, 588, 667 |
| Hsp92II | CATG | 2 cut(s) | 334, 714 |
| Kzo9I | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| LguI | GCTCTTC | 1 cut(s) | 648 |
| LmnI | GCTCC | 1 cut(s) | 73 |
| LpnPI | CCDG | 6 cut(s) | 66, 287, 382, 396, 568, 693 |
| LweI | GCATC | 2 cut(s) | 282, 388 |
| MaeI | CTAG | 1 cut(s) | 191 |
| MaeII | ACGT | 3 cut(s) | 129, 588, 667 |
| MaeIII | GTNAC | 2 cut(s) | 125, 414 |
| MalI | GATC | 7 cut(s) | 82, 171, 328, 359, 388, 409, 510 |
| MboI | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| MboII | GAAGA | 6 cut(s) | 75, 251, 403, 625, 665, 707 |
| MflI | RGATCY | 1 cut(s) | 80 |
| MlyI | GAGTC | 2 cut(s) | 155, 685 |
| MnlI | CCTC | 6 cut(s) | 25, 94, 125, 145, 304, 631 |
| Mph1103I | ATGCAT | 1 cut(s) | 275 |
| MseI | TTAA | 2 cut(s) | 342, 515 |
| MslI | CAYNNNNRTG | 2 cut(s) | 10, 540 |
| MspI | CCGG | 1 cut(s) | 555 |
| MvnI | CGCG | 1 cut(s) | 566 |
| NdeII | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| NlaIII | CATG | 2 cut(s) | 334, 714 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| NruI | TCGCGA | 1 cut(s) | 566 |
| NsiI | ATGCAT | 1 cut(s) | 275 |
| NspV | TTCGAA | 1 cut(s) | 59 |
| OliI | CACNNNNGTG | 1 cut(s) | 10 |
| PciSI | GCTCTTC | 1 cut(s) | 648 |
| PfeI | GAWTC | 2 cut(s) | 223, 619 |
| Ple19I | CGATCG | 1 cut(s) | 389 |
| PleI | GAGTC | 2 cut(s) | 154, 684 |
| PpsI | GAGTC | 2 cut(s) | 154, 684 |
| Ppu21I | YACGTR | 1 cut(s) | 668 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| PspPI | GGNCC | 1 cut(s) | 525 |
| PstNI | CAGNNNCTG | 1 cut(s) | 53 |
| PsuI | RGATCY | 1 cut(s) | 80 |
| PvuI | CGATCG | 1 cut(s) | 389 |
| RruI | TCGCGA | 1 cut(s) | 566 |
| RsaI | GTAC | 2 cut(s) | 132, 365 |
| RsaNI | GTAC | 2 cut(s) | 131, 364 |
| RseI | CAYNNNNRTG | 2 cut(s) | 10, 540 |
| SapI | GCTCTTC | 1 cut(s) | 648 |
| SaqAI | TTAA | 2 cut(s) | 342, 515 |
| Sau3AI | GATC | 7 cut(s) | 80, 169, 326, 357, 386, 407, 508 |
| Sau96I | GGNCC | 1 cut(s) | 525 |
| SchI | GAGTC | 2 cut(s) | 155, 685 |
| SfaNI | GCATC | 2 cut(s) | 282, 388 |
| SfuI | TTCGAA | 1 cut(s) | 59 |
| SinI | GGWCC | 1 cut(s) | 525 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 10, 540 |
| SmlI | CTYRAG | 1 cut(s) | 118 |
| SmoI | CTYRAG | 1 cut(s) | 118 |
| SnaBI | TACGTA | 1 cut(s) | 668 |
| SpeI | ACTAGT | 1 cut(s) | 190 |
| SsiI | CCGC | 1 cut(s) | 501 |
| SspMI | CTAG | 1 cut(s) | 191 |
| StyI | CCWWGG | 1 cut(s) | 8 |
| TaaI | ACNGT | 3 cut(s) | 67, 293, 649 |
| TaiI | ACGT | 3 cut(s) | 132, 591, 670 |
| TaqI | TCGA | 3 cut(s) | 59, 389, 547 |
| TatI | WGTACW | 1 cut(s) | 363 |
| TfiI | GAWTC | 2 cut(s) | 223, 619 |
| Tru1I | TTAA | 2 cut(s) | 342, 515 |
| Tru9I | TTAA | 2 cut(s) | 342, 515 |
| TscAI | CASTG | 1 cut(s) | 547 |
| TspDTI | ATGAA | 3 cut(s) | 75, 632, 714 |
| TspRI | CASTG | 1 cut(s) | 547 |
| VpaK11BI | GGWCC | 1 cut(s) | 525 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 15 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 1 cut(s) | 191 |
| Zsp2I | ATGCAT | 1 cut(s) | 275 |