Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0003092

Physical Location & Seq

Genomic Coordinates Reverse (-)

40514901 .. 40519332

Gene Structure 4432 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG214900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGTCAAAGATAGTACTGCAGCTTCAGTCTTCTCTGACTGGATCGAGTTCTCGAGGGTTCCTATGTGGGAGCAGTGTACTTGTTGAAATGAAAGCAGAAGAAGCTAATCTCCTTGAATCTGCCTGCTTTGGTGTTAGAGTGATGGAGAATAAGCACTGGTTTCAGTTGTGTGTGGAAGAGGCCTTTTACCTATGCCATTCTCTAAAATGCCTCATGATAGTTGAAGATAAGTGCTCCAAGAATGATAACCAGCTATGGAAGTCAGGAGTTAAATATGGTGTGGACTTTGTTGCCTACCGCCATCATCCAGCTCTAGTTCACTCTGAATATGCGGTGATTGTATTGTCAGAGGAACATAGTGATGGAAATAAGCGACTGACGGAGTGGTTAGATATTCATTGTACTACTCGTCTTTGTGGAGGTGTTGCAAAGACATTGTTAGTTCTTTATATCAGCAGAAATGGTCATAGTGTGGACTCCCCATCATGTCTGGAGAGATGTACTGTTGAAGAGCGTACAGTCACAAGATGGAAGCCAGAACAATGCCGTGATGATGATAGATTAGTTGAAAGTGAGAACGGAACCAAATTGAAGCAACTTGGTGCCAATATATTCTCCTGCTGTACTCACTCTACTGGCTCAGAACCTCTTGACAGAGATGATTCTGAACATATATTGAATGATGATGAGCTCTCTCTCAACACTATGGCAGACGATGGAAGGGATGAGGAGGTAGCTGACAAATTAATGATTATGCATGAAGAATGTTTAGATGAAGAGTATACCAATGACTATGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAAACCACTATGCTTCTTGTGCCACATCCAAGATGCAACCCAGGAGGTCTAGTGTATCCAAGGCTTACTGGGATGATGAGATGGGCATGGCTGTCGTATTTCCAAAGGGTAGAATTTGGAGGACCATGGGAATCTCTCGTAATGGCAAGCTCTATATCTCCATTGAGGAAGTTTTGTATTTGGTTGAAATAGGGGCTTTGCTTCTCTTGGATGGTAGTGATACTAGTATTTCCTTGGAAGATATATATACGAAGGTTTCAGATGGAAAGAATGAGTTCTTGTTGGAGGAATTTAGAGCTTATAGGCAACTCAGATACCTTGGTTACATTGTTGCGAGACATGGTATTCCTTGGTCTAGGAAGGGTGTCAAGACAAATTGTGAATCTGTTTCTTCTCAAGTTTGTCCTGAAGCTGAAGAGATTAGGTCTGTCAATGGATTGTTCAATGGGATACAGATTAATGAAGTGAGGGCAGTTTTTGATAATTATCTTCCAAACAGCAAGTTCAAGAAATCCTCTCCCGGTGATCCAAATTTTGTGCTCTGTTTTACTAGGGGCCATCCACCTTCCAAACCAGACCTTGAAGCCCTTGAGAGACAATTTGGTAGCACTCCTTTGAAGTTTTGTCATGTGGAGGAAGATTGTGTGAGTTTCTATTCCTTTGACAAAGTGGAACTTCATGTCGTCTCATGTCATCTGCAAAAATTTCGACATCCCTCTCTAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000213 GO:0000214 GO:0000278 GO:0000281 GO:0000379 GO:0000394 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0004549 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006388 GO:0006396 GO:0006399 GO:0006725 GO:0006807 GO:0007049 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0008356 GO:0008380 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0016892 GO:0016894 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0061640 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:0140101 GO:1901360 GO:1902494 GO:1902555 GO:1903047 GO:1905348

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

tRNA_int_endo tRNA_int_endo_N

Protein Analysis

519

Amino Acids

59.13

Weight (kDa)

5.0

Isoelectric Point (pI)

57.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_int_endo_N	PF02778	18 - 86	1.8e-07	tRNA intron endonuclease, N-terminal domain
tRNA_int_endo	PF01974	86 - 155	9.9e-11	tRNA intron endonuclease, catalytic C-terminal domain
tRNA_int_end_N2	PF12928	292 - 346	2.8e-14	tRNA-splicing endonuclease subunit sen54 N-term

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003092)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45577 AT3G45590 AT3G45590 AT5G60230 AT5G60230
fragaria_vesca	FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101 FvH4_2g14101
rosa_chinensis	RchiOBHm_Chr6g0276201
rosa_laevigata	RLG00000013409 RLG00000025847
rosa_multiflora	Rmu_sc0002063.1_g000007 Rmu_sc0004048.1_g000016 Rmu_sc0008839.1_g000006
rosa_roxburghii	Rroxscaffold_2G00125430 Rroxscaffold_7G00192490
rosa_rugosa	Rorug06G0098800 Rorug06G0098900 Rorug06G0098900 Rorug06G0099000 Rorug06G0099100 Rorug06G0099200 Rorug06G0099300 Rorug06G0124300
rosa_samantha	Rh1AG171200 Rh3BG017000 Rh3DG017300 Rh5BG538000 Rh5CG561900 Rh6AG210700 Rh6BG214900 Rh6DG207200
rosa_wichuraiana	Rw6G018360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	602
AccI	GTMKAC	1 cut(s)	782
AciI	CCGC	2 cut(s)	298, 332
AclWI	GGATC	2 cut(s)	49, 1352
AcsI	RAATTY	4 cut(s)	945, 1121, 1363, 1535
AcuI	CTGAAG	3 cut(s)	8, 1260, 1266
AfaI	GTAC	6 cut(s)	15, 78, 403, 502, 517, 625
AhlI	ACTAGT	1 cut(s)	1055
AjnI	CCWGG	1 cut(s)	872
AluBI	AGCT	9 cut(s)	22, 104, 253, 311, 691, 737, 982, 1130, 1244
AluI	AGCT	9 cut(s)	22, 104, 253, 311, 691, 737, 982, 1130, 1244
Alw21I	GWGCWC	3 cut(s)	236, 693, 1374
Alw26I	GTCTC	3 cut(s)	1162, 1420, 1522
AlwI	GGATC	2 cut(s)	49, 1352
Ama87I	CYCGRG	1 cut(s)	51
AoxI	GGCC	2 cut(s)	180, 1387
ApeKI	GCWGC	1 cut(s)	19
ApoI	RAATTY	4 cut(s)	945, 1121, 1363, 1535
AseI	ATTAAT	2 cut(s)	746, 1290
Asp700I	GAANNNNTTC	1 cut(s)	1106
AspS9I	GGNCC	2 cut(s)	954, 1387
AsuC2I	CCSGG	1 cut(s)	1353
AsuHPI	GGTGA	2 cut(s)	346, 1367
AvaI	CYCGRG	1 cut(s)	51
AvaII	GGWCC	1 cut(s)	954
BaeI	ACNNNNGTAYC	2 cut(s)	1138, 1171
BanI	GGYRCC	1 cut(s)	602
BanII	GRGCYC	1 cut(s)	693
BarI	GAAGNNNNNNTAC	1 cut(s)	38
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	3 cut(s)	236, 693, 1374
BbvI	GCAGC	1 cut(s)	31
BceAI	ACGGC	1 cut(s)	531
BcgI	CGANNNNNNTGC	4 cut(s)	907, 941, 1520, 1554
BciT130I	CCWGG	1 cut(s)	874
BciVI	GTATCC	2 cut(s)	898, 1275
BcnI	CCSGG	1 cut(s)	1353
BcoDI	GTCTC	3 cut(s)	1162, 1420, 1522
BcuI	ACTAGT	1 cut(s)	1055
BfaI	CTAG	5 cut(s)	314, 882, 1056, 1188, 1383
BfmI	CTRYAG	1 cut(s)	17
BfuI	GTATCC	2 cut(s)	898, 1275
BisI	GCNGC	1 cut(s)	20
BlsI	GCNGC	1 cut(s)	21
BmcAI	AGTACT	1 cut(s)	15
Bme1390I	CCNGG	2 cut(s)	874, 1353
Bme18I	GGWCC	1 cut(s)	954
BmeT110I	CYCGRG	1 cut(s)	51
BmgT120I	GGNCC	2 cut(s)	954, 1387
BmiI	GGNNCC	4 cut(s)	59, 583, 604, 1388
BmrFI	CCNGG	2 cut(s)	874, 1353
BmrI	ACTGGG	1 cut(s)	910
BmsI	GCATC	1 cut(s)	855
BmuI	ACTGGG	1 cut(s)	910
BpiI	GAAGAC	1 cut(s)	21
BpmI	CTGGAG	1 cut(s)	512
BpuEI	CTTGAG	2 cut(s)	1212, 1442
BpuMI	CCSGG	1 cut(s)	1353
BsaBI	GATNNNNATC	1 cut(s)	1317
BsaJI	CCNNGG	6 cut(s)	872, 891, 957, 1065, 1150, 1181
BsaXI	ACNNNNNCTCC	2 cut(s)	868, 898
Bse1I	ACTGG	4 cut(s)	43, 161, 640, 905
Bse8I	GATNNNNATC	1 cut(s)	1317
BseBI	CCWGG	1 cut(s)	874
BseDI	CCNNGG	6 cut(s)	872, 891, 957, 1065, 1150, 1181
BseGI	GGATG	7 cut(s)	304, 730, 857, 910, 1048, 1390, 1543
BseJI	GATNNNNATC	1 cut(s)	1317
BseMII	CTCAG	2 cut(s)	654, 1156
BseNI	ACTGG	4 cut(s)	43, 161, 640, 905
BseRI	GAGGAG	1 cut(s)	743
BseXI	GCAGC	1 cut(s)	31
BshFI	GGCC	2 cut(s)	182, 1389
BshNI	GGYRCC	1 cut(s)	602
BsiHKAI	GWGCWC	3 cut(s)	236, 693, 1374
BsiHKCI	CYCGRG	1 cut(s)	51
BsiSI	CCGG	1 cut(s)	1353
BsmAI	GTCTC	3 cut(s)	1162, 1420, 1522
BsmBI	CGTCTC	1 cut(s)	1522
BsnI	GGCC	2 cut(s)	182, 1389
BsoBI	CYCGRG	1 cut(s)	51
Bsp1286I	GDGCHC	3 cut(s)	236, 693, 1374
Bsp143I	GATC	2 cut(s)	41, 1357
Bsp19I	CCATGG	1 cut(s)	957
BspACI	CCGC	2 cut(s)	298, 332
BspANI	GGCC	2 cut(s)	182, 1389
BspCNI	CTCAG	2 cut(s)	653, 1155
BspHI	TCATGA	1 cut(s)	213
BspLI	GGNNCC	4 cut(s)	59, 583, 604, 1388
BspMAI	CTGCAG	1 cut(s)	21
BspPI	GGATC	2 cut(s)	49, 1352
BspQI	GCTCTTC	1 cut(s)	504
BspT107I	GGYRCC	1 cut(s)	602
BsrI	ACTGG	4 cut(s)	43, 161, 640, 905
BssECI	CCNNGG	6 cut(s)	872, 891, 957, 1065, 1150, 1181
BssMI	GATC	2 cut(s)	41, 1357
BssNAI	GTATAC	1 cut(s)	783
BssT1I	CCWWGG	5 cut(s)	891, 957, 1065, 1150, 1181
Bst1107I	GTATAC	1 cut(s)	783
Bst2UI	CCWGG	1 cut(s)	874
Bst4CI	ACNGT	2 cut(s)	505, 520
Bst6I	CTCTTC	5 cut(s)	171, 504, 771, 822, 1242
BstC8I	GCNNGC	2 cut(s)	124, 980
BstDEI	CTNAG	2 cut(s)	640, 1142
BstDSI	CCRYGG	1 cut(s)	957
BstF5I	GGATG	7 cut(s)	304, 730, 857, 910, 1048, 1390, 1543
BstKTI	GATC	2 cut(s)	44, 1360
BstMAI	GTCTC	3 cut(s)	1162, 1420, 1522
BstMBI	GATC	2 cut(s)	41, 1357
BstMWI	GCNNNNNNNGC	2 cut(s)	101, 851
BstNI	CCWGG	1 cut(s)	874
BstSCI	CCNGG	2 cut(s)	872, 1351
BstSFI	CTRYAG	1 cut(s)	17
BstV1I	GCAGC	1 cut(s)	31
BstV2I	GAAGAC	1 cut(s)	21
BstZ17I	GTATAC	1 cut(s)	783
BsuI	GTATCC	2 cut(s)	898, 1275
BsuRI	GGCC	2 cut(s)	182, 1389
BtgI	CCRYGG	1 cut(s)	957
BtsCI	GGATG	7 cut(s)	304, 730, 857, 910, 1048, 1390, 1543
BtsI	GCAGTG	1 cut(s)	79
BtsIMutI	CAGTG	2 cut(s)	79, 154
Cac8I	GCNNGC	2 cut(s)	124, 980
CciI	TCATGA	1 cut(s)	213
Cfr13I	GGNCC	2 cut(s)	954, 1387
Csp6I	GTAC	6 cut(s)	14, 77, 402, 501, 516, 624
CviAII	CATG	9 cut(s)	214, 486, 758, 919, 958, 1172, 1460, 1511, 1521
CviQI	GTAC	6 cut(s)	14, 77, 402, 501, 516, 624
DdeI	CTNAG	2 cut(s)	640, 1142
DpnI	GATC	2 cut(s)	43, 1359
DpnII	GATC	2 cut(s)	41, 1357
Eam1104I	CTCTTC	5 cut(s)	171, 504, 771, 822, 1242
EarI	CTCTTC	5 cut(s)	171, 504, 771, 822, 1242
Ecl136II	GAGCTC	1 cut(s)	691
Eco130I	CCWWGG	5 cut(s)	891, 957, 1065, 1150, 1181
Eco147I	AGGCCT	1 cut(s)	182
Eco24I	GRGCYC	1 cut(s)	693
Eco47I	GGWCC	1 cut(s)	954
Eco53kI	GAGCTC	1 cut(s)	691
Eco57I	CTGAAG	3 cut(s)	8, 1260, 1266
Eco88I	CYCGRG	1 cut(s)	51
EcoICRI	GAGCTC	1 cut(s)	691
EcoRII	CCWGG	1 cut(s)	872
EcoT14I	CCWWGG	5 cut(s)	891, 957, 1065, 1150, 1181
EcoT22I	ATGCAT	1 cut(s)	759
EcoT38I	GRGCYC	1 cut(s)	693
ErhI	CCWWGG	5 cut(s)	891, 957, 1065, 1150, 1181
Esp3I	CGTCTC	1 cut(s)	1522
FaeI	CATG	9 cut(s)	217, 489, 761, 922, 961, 1175, 1463, 1514, 1524
FalI	AAGNNNNNCTT	2 cut(s)	1491, 1523
FatI	CATG	9 cut(s)	213, 485, 757, 918, 957, 1171, 1459, 1510, 1520
FblI	GTMKAC	1 cut(s)	782
Fnu4HI	GCNGC	1 cut(s)	20
FokI	GGATG	7 cut(s)	291, 737, 844, 917, 1055, 1377, 1530
FriOI	GRGCYC	1 cut(s)	693
Fsp4HI	GCNGC	1 cut(s)	20
FspBI	CTAG	5 cut(s)	314, 882, 1056, 1188, 1383
GluI	GCNGC	1 cut(s)	20
GsuI	CTGGAG	1 cut(s)	512
HaeIII	GGCC	2 cut(s)	182, 1389
HapII	CCGG	1 cut(s)	1353
Hin1II	CATG	9 cut(s)	217, 489, 761, 922, 961, 1175, 1463, 1514, 1524
HinfI	GANTC	5 cut(s)	116, 476, 662, 963, 1214
HpaII	CCGG	1 cut(s)	1353
HphI	GGTGA	2 cut(s)	346, 1367
Hpy166II	GTNNAC	5 cut(s)	77, 283, 319, 475, 783
Hpy188I	TCNGA	7 cut(s)	36, 325, 349, 643, 667, 1093, 1145
Hpy188III	TCNNGA	8 cut(s)	51, 214, 264, 491, 650, 1201, 1238, 1339
Hpy8I	GTNNAC	5 cut(s)	77, 283, 319, 475, 783
HpyAV	CCTTC	4 cut(s)	714, 1078, 1186, 1407
HpyCH4III	ACNGT	2 cut(s)	505, 520
HpyCH4V	TGCA	5 cut(s)	19, 428, 757, 868, 1531
HpyF10VI	GCNNNNNNNGC	2 cut(s)	101, 851
HpyF3I	CTNAG	2 cut(s)	640, 1142
Hsp92II	CATG	9 cut(s)	217, 489, 761, 922, 961, 1175, 1463, 1514, 1524
Kzo9I	GATC	2 cut(s)	41, 1357
LguI	GCTCTTC	1 cut(s)	504
LmnI	GCTCC	2 cut(s)	69, 239
Lsp1109I	GCAGC	1 cut(s)	31
LweI	GCATC	1 cut(s)	855
MaeI	CTAG	5 cut(s)	314, 882, 1056, 1188, 1383
MaeIII	GTNAC	2 cut(s)	520, 1154
MalI	GATC	2 cut(s)	43, 1359
MboI	GATC	2 cut(s)	41, 1357
MhlI	GDGCHC	3 cut(s)	236, 693, 1374
MluCI	AATT	9 cut(s)	587, 743, 945, 1121, 1207, 1315, 1363, 1430, 1535
MlyI	GAGTC	1 cut(s)	470
MmeI	TCCRAC	1 cut(s)	1095
Mph1103I	ATGCAT	1 cut(s)	759
MroXI	GAANNNNTTC	1 cut(s)	1106
MseI	TTAA	3 cut(s)	270, 746, 1290
MslI	CAYNNNNRTG	1 cut(s)	360
MspI	CCGG	1 cut(s)	1353
MspR9I	CCNGG	2 cut(s)	874, 1353
MvaI	CCWGG	1 cut(s)	874
MwoI	GCNNNNNNNGC	2 cut(s)	101, 851
NciI	CCSGG	1 cut(s)	1353
NcoI	CCATGG	1 cut(s)	957
NdeII	GATC	2 cut(s)	41, 1357
NlaIII	CATG	9 cut(s)	217, 489, 761, 922, 961, 1175, 1463, 1514, 1524
NlaIV	GGNNCC	4 cut(s)	59, 583, 604, 1388
NmuCI	GTSAC	1 cut(s)	520
NsiI	ATGCAT	1 cut(s)	759
PaeR7I	CTCGAG	1 cut(s)	51
PagI	TCATGA	1 cut(s)	213
PceI	AGGCCT	1 cut(s)	182
PciSI	GCTCTTC	1 cut(s)	504
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	4 cut(s)	116, 662, 963, 1214
PkrI	GCNGC	1 cut(s)	21
PleI	GAGTC	1 cut(s)	470
PpsI	GAGTC	1 cut(s)	470
PshBI	ATTAAT	2 cut(s)	746, 1290
Psp124BI	GAGCTC	1 cut(s)	693
Psp6I	CCWGG	1 cut(s)	872
PspGI	CCWGG	1 cut(s)	872
PspN4I	GGNNCC	4 cut(s)	59, 583, 604, 1388
PspPI	GGNCC	2 cut(s)	954, 1387
PstI	CTGCAG	1 cut(s)	21
RsaI	GTAC	6 cut(s)	15, 78, 403, 502, 517, 625
RsaNI	GTAC	6 cut(s)	14, 77, 402, 501, 516, 624
RseI	CAYNNNNRTG	1 cut(s)	360
SacI	GAGCTC	1 cut(s)	693
SapI	GCTCTTC	1 cut(s)	504
SaqAI	TTAA	3 cut(s)	270, 746, 1290
SatI	GCNGC	1 cut(s)	20
Sau3AI	GATC	2 cut(s)	41, 1357
Sau96I	GGNCC	2 cut(s)	954, 1387
ScaI	AGTACT	1 cut(s)	15
SchI	GAGTC	1 cut(s)	470
ScrFI	CCNGG	2 cut(s)	874, 1353
SduI	GDGCHC	3 cut(s)	236, 693, 1374
SfaNI	GCATC	1 cut(s)	855
SfcI	CTRYAG	1 cut(s)	17
Sfr274I	CTCGAG	1 cut(s)	51
SinI	GGWCC	1 cut(s)	954
SlaI	CTCGAG	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	360
SmlI	CTYRAG	3 cut(s)	51, 1227, 1421
SmoI	CTYRAG	3 cut(s)	51, 1227, 1421
SpeI	ACTAGT	1 cut(s)	1055
Sse9I	AATT	9 cut(s)	587, 743, 945, 1121, 1207, 1315, 1363, 1430, 1535
SseBI	AGGCCT	1 cut(s)	182
SsiI	CCGC	2 cut(s)	298, 332
SspMI	CTAG	5 cut(s)	314, 882, 1056, 1188, 1383
SstI	GAGCTC	1 cut(s)	693
StuI	AGGCCT	1 cut(s)	182
StyD4I	CCNGG	2 cut(s)	872, 1351
StyI	CCWWGG	5 cut(s)	891, 957, 1065, 1150, 1181
TaaI	ACNGT	2 cut(s)	505, 520
TaqI	TCGA	3 cut(s)	44, 52, 1540
TasI	AATT	9 cut(s)	587, 743, 945, 1121, 1207, 1315, 1363, 1430, 1535
TatI	WGTACW	5 cut(s)	13, 76, 401, 500, 623
TfiI	GAWTC	4 cut(s)	116, 662, 963, 1214
Tru1I	TTAA	3 cut(s)	270, 746, 1290
Tru9I	TTAA	3 cut(s)	270, 746, 1290
TscAI	CASTG	2 cut(s)	79, 161
TseFI	GTSAC	1 cut(s)	520
TseI	GCWGC	1 cut(s)	19
Tsp45I	GTSAC	1 cut(s)	520
TspDTI	ATGAA	7 cut(s)	104, 386, 774, 789, 813, 1308, 1499
TspGWI	ACGGA	2 cut(s)	395, 594
TspRI	CASTG	2 cut(s)	79, 161
VpaK11BI	GGWCC	1 cut(s)	954
VspI	ATTAAT	2 cut(s)	746, 1290
XapI	RAATTY	4 cut(s)	945, 1121, 1363, 1535
XhoI	CTCGAG	1 cut(s)	51
XmiI	GTMKAC	1 cut(s)	782
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	5 cut(s)	314, 882, 1056, 1188, 1383
ZrmI	AGTACT	1 cut(s)	15
Zsp2I	ATGCAT	1 cut(s)	759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.