AT3G50640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006528
Physical Location & Seq
Reverse (-)
18803665 .. 18804731
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50640.1

Sequence Viewer

Length: 501 bp
ATGGCACGCTTAGAAATGCACAACTCCATGAATGGTGCTGCTAGAATCTCTTTTTCTAACGAGTTTGTCGAGATCAGATCGGAGAAGAGTAATGCTAAGAGCAACAACATCAACTCGAGAAGCTCTTTCTCCATGCCCTCAGCTGATTTCGCTTTCTCAGTCACCGATTACTCGATGATTCCCGCCGATGAAATCTTCCTCAAAGGAAAGATTTTACCTTTTAAGGAGACCAGTCACGTCCATAGGACCTTAGGAGAAGAGCTTCTTACTGAAGAGGAGGGTTCTATGGTCGATGGTAACACTTTCTCCCTCAGGCCAATATTGTTGTCTTCTTCATCCTTTTCCACAAAGGGCACGTGGAGGGAGCTTTTGGGACTTAAGAGGACCCATGTTAGATCTAAGAAGACTGATAAAGTCAATGAGGAAGTGTTAAGTCAAGATCACAAAATAATCTCAGGAAATGTTGCAACGAGGGAGTGTCAAGTTGCAGATACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.6

Weight (kDa)

6.98

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 291
AcvI CACGTG 1 cut(s) 357
AflII CTTAAG 1 cut(s) 377
AjiI CACGTC 1 cut(s) 238
AluBI AGCT 4 cut(s) 123, 143, 262, 367
AluI AGCT 4 cut(s) 123, 143, 262, 367
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 246, 384
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 2 cut(s) 246, 384
AxyI CCTNAGG 2 cut(s) 250, 311
BaeGI GKGCMC 1 cut(s) 356
BbrPI CACGTG 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 321, 410
BbvCI CCTCAGC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 42
BfrI CTTAAG 1 cut(s) 377
BglII AGATCT 1 cut(s) 395
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme18I GGWCC 2 cut(s) 246, 384
BmeT110I CYCGRG 1 cut(s) 115
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 246, 384
BmiI GGNNCC 1 cut(s) 386
BpiI GAAGAC 2 cut(s) 321, 410
Bpu10I CCTNAGC 1 cut(s) 139
BsaAI YACGTR 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bse1I ACTGG 1 cut(s) 231
Bse21I CCTNAGG 2 cut(s) 250, 311
BseGI GGATG 1 cut(s) 335
BseMII CTCAG 4 cut(s) 153, 171, 325, 468
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 1 cut(s) 25
BshFI GGCC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 115
BslFI GGGAC 1 cut(s) 387
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 387
BsnI GGCC 1 cut(s) 316
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 4 cut(s) 72, 77, 395, 439
BspACI CCGC 1 cut(s) 183
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 4 cut(s) 152, 170, 324, 467
BspLI GGNNCC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 221
BsrI ACTGG 1 cut(s) 231
BssMI GATC 4 cut(s) 72, 77, 395, 439
Bst6I CTCTTC 3 cut(s) 80, 252, 267
BstAFI CTTAAG 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 8 cut(s) 10, 96, 139, 157, 250, 311, 399, 454
BstF5I GGATG 1 cut(s) 335
BstKTI GATC 4 cut(s) 75, 80, 398, 442
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 4 cut(s) 72, 77, 395, 439
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstSLI GKGCMC 1 cut(s) 356
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 321, 410
BstX2I RGATCY 1 cut(s) 395
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 2 cut(s) 250, 311
BsuRI GGCC 1 cut(s) 316
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 1 cut(s) 335
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 246, 384
CviAII CATG 3 cut(s) 28, 133, 389
CviJI RGCY 5 cut(s) 123, 143, 262, 316, 367
CviKI_1 RGCY 5 cut(s) 123, 143, 262, 316, 367
DdeI CTNAG 8 cut(s) 10, 96, 139, 157, 250, 311, 399, 454
DpnI GATC 4 cut(s) 74, 79, 397, 441
DpnII GATC 4 cut(s) 72, 77, 395, 439
Eam1104I CTCTTC 3 cut(s) 80, 252, 267
EarI CTCTTC 3 cut(s) 80, 252, 267
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 246, 384
Eco57I CTGAAG 1 cut(s) 291
Eco72I CACGTG 1 cut(s) 357
Eco81I CCTNAGG 2 cut(s) 250, 311
Eco88I CYCGRG 1 cut(s) 115
EcoO109I RGGNCCY 2 cut(s) 246, 384
FaeI CATG 3 cut(s) 31, 136, 392
FaiI YATR 5 cut(s) 29, 134, 243, 287, 390
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 1 cut(s) 387
FatI CATG 3 cut(s) 27, 132, 388
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 1 cut(s) 42
GluI GCNGC 1 cut(s) 39
HaeIII GGCC 1 cut(s) 316
Hin1II CATG 3 cut(s) 31, 136, 392
HinfI GANTC 2 cut(s) 45, 178
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 77, 82
Hpy188III TCNNGA 4 cut(s) 70, 117, 437, 456
HpyCH4IV ACGT 2 cut(s) 237, 356
HpyCH4V TGCA 3 cut(s) 19, 467, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 8 cut(s) 10, 96, 139, 157, 250, 311, 399, 454
HpySE526I ACGT 2 cut(s) 237, 356
Hsp92II CATG 3 cut(s) 31, 136, 392
Kzo9I GATC 4 cut(s) 72, 77, 395, 439
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 3 cut(s) 244, 298, 441
Lsp1109I GCAGC 1 cut(s) 25
MaeI CTAG 1 cut(s) 42
MaeII ACGT 2 cut(s) 237, 356
MaeIII GTNAC 3 cut(s) 160, 233, 296
MalI GATC 4 cut(s) 74, 79, 397, 441
MboI GATC 4 cut(s) 72, 77, 395, 439
MboII GAAGA 7 cut(s) 97, 187, 269, 284, 321, 324, 415
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 356
MnlI CCTC 9 cut(s) 148, 209, 268, 271, 320, 354, 375, 415, 465
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 3 cut(s) 222, 378, 431
MspA1I CMGCKG 1 cut(s) 143
MspCI CTTAAG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 4 cut(s) 72, 77, 395, 439
NlaIII CATG 3 cut(s) 31, 136, 392
NlaIV GGNNCC 1 cut(s) 386
NmuCI GTSAC 2 cut(s) 160, 233
PaeR7I CTCGAG 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 45, 178
PkrI GCNGC 1 cut(s) 40
PmaCI CACGTG 1 cut(s) 357
PmlI CACGTG 1 cut(s) 357
Ppu21I YACGTR 1 cut(s) 357
PpuMI RGGWCCY 2 cut(s) 246, 384
Psp5II RGGWCCY 2 cut(s) 246, 384
PspCI CACGTG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 2 cut(s) 246, 384
PspPPI RGGWCCY 2 cut(s) 246, 384
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 222, 378, 431
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 4 cut(s) 72, 77, 395, 439
Sau96I GGNCC 2 cut(s) 246, 384
SduI GDGCHC 1 cut(s) 356
SetI ASST 8 cut(s) 125, 145, 220, 240, 251, 264, 359, 369
Sfr274I CTCGAG 1 cut(s) 115
SinI GGWCC 2 cut(s) 246, 384
SlaI CTCGAG 1 cut(s) 115
SmlI CTYRAG 2 cut(s) 115, 377
SmoI CTYRAG 2 cut(s) 115, 377
SsiI CCGC 1 cut(s) 183
SspI AATATT 1 cut(s) 321
SspMI CTAG 1 cut(s) 42
TaiI ACGT 2 cut(s) 240, 359
TaqI TCGA 4 cut(s) 69, 116, 173, 291
TfiI GAWTC 2 cut(s) 45, 178
Tru1I TTAA 3 cut(s) 222, 378, 431
Tru9I TTAA 3 cut(s) 222, 378, 431
TseFI GTSAC 2 cut(s) 160, 233
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 2 cut(s) 160, 233
TspDTI ATGAA 3 cut(s) 44, 204, 324
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 246, 384
XhoI CTCGAG 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.