RLG00000017414

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006528
Physical Location & Seq
Reverse (-)
16335052 .. 16335699
648 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017414

Sequence Viewer

Length: 546 bp
ATGGCCTGTTTACACACAATGTACAACTCAGAGCACAATGGTCAGTACTGTAATGGTGGTAGTGGTGGTGCTCCAATGAGCCCTAGAATTTCCTTCTCTAATGACTTTGCTGATGCGCAACATGTGATTAAGCATGAGAGGAGCTCAAGGGAGGCACCACTGTCTTCAGATTTTGAGTTCTCAGTGACCAACTACTCCATGATGAGTGCTGATGAGCTTTTCTCTAAGGGGAGGTTGCTGCCTTACAAGGGAAGCAACAAGAGCCAGATGCAGAGGGGTACTACCACTCTTAGAGACGAGCTGCTTGTGGAGGATCGGGAGGACGACGTATCGTTTCATAGGCAGCCGAAGGGGTCATCGACGAGGTGGAAGGGGCTTCTGGGGCGGAAGAGGACTGCCCACATTGGGTCCAAGAAAGCTGAGAGAGGTAGTGATGGCTCTTCTATGGAAGGAAGGAGGTCTGGCCTTGTGCATGAGAGTTCTTCTTCACAGGAGGTGTTGAATGAAGGAGGGTCAAGTTGGAGGGGGTTTGAGATTGGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.01

Weight (kDa)

7.85

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 1 cut(s) 385
AclWI GGATC 1 cut(s) 321
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 3 cut(s) 23, 47, 280
AfiI CCNNNNNNNGG 2 cut(s) 248, 405
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 1 cut(s) 502
AluBI AGCT 4 cut(s) 144, 217, 301, 419
AluI AGCT 4 cut(s) 144, 217, 301, 419
Alw21I GWGCWC 3 cut(s) 36, 73, 146
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 1 cut(s) 321
AoxI GGCC 2 cut(s) 3, 463
ApeKI GCWGC 3 cut(s) 238, 301, 343
ApoI RAATTY 1 cut(s) 87
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 408
AvaII GGWCC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 2 cut(s) 83, 146
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 3 cut(s) 36, 73, 146
BbvI GCAGC 3 cut(s) 225, 288, 355
BccI CCATC 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 84
BisI GCNGC 3 cut(s) 239, 302, 344
BlsI GCNGC 3 cut(s) 240, 303, 345
BmcAI AGTACT 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 408
BmgT120I GGNCC 1 cut(s) 408
BmiI GGNNCC 2 cut(s) 156, 409
BmsI GCATC 2 cut(s) 103, 258
BpiI GAAGAC 1 cut(s) 156
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 206, 238
BpuEI CTTGAG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 405
BseLI CCNNNNNNNGG 2 cut(s) 248, 405
BseMII CTCAG 3 cut(s) 42, 195, 411
BseRI GAGGAG 1 cut(s) 154
BseXI GCAGC 3 cut(s) 225, 288, 355
BshFI GGCC 2 cut(s) 5, 465
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 3 cut(s) 36, 73, 146
BslI CCNNNNNNNGG 2 cut(s) 248, 405
BsmAI GTCTC 1 cut(s) 288
BsmBI CGTCTC 1 cut(s) 288
BsnI GGCC 2 cut(s) 5, 465
Bsp1286I GDGCHC 4 cut(s) 36, 73, 83, 146
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 1 cut(s) 313
BspACI CCGC 1 cut(s) 385
BspANI GGCC 2 cut(s) 5, 465
BspCNI CTCAG 3 cut(s) 41, 194, 412
BspLI GGNNCC 2 cut(s) 156, 409
BspPI GGATC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 21
BssMI GATC 1 cut(s) 313
Bst4CI ACNGT 2 cut(s) 50, 162
Bst6I CTCTTC 2 cut(s) 383, 445
BstAUI TGTACA 1 cut(s) 21
BstDEI CTNAG 5 cut(s) 28, 181, 225, 290, 420
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 1 cut(s) 316
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 261, 382
BstNSI RCATGY 1 cut(s) 125
BstV1I GCAGC 3 cut(s) 225, 288, 355
BstV2I GAAGAC 1 cut(s) 156
BsuRI GGCC 2 cut(s) 5, 465
BtsIMutI CAGTG 2 cut(s) 158, 189
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 408
Csp6I GTAC 3 cut(s) 22, 46, 279
CviAII CATG 4 cut(s) 122, 134, 199, 473
CviQI GTAC 3 cut(s) 22, 46, 279
DdeI CTNAG 5 cut(s) 28, 181, 225, 290, 420
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
Eam1104I CTCTTC 2 cut(s) 383, 445
EarI CTCTTC 2 cut(s) 383, 445
EciI GGCGGA 1 cut(s) 400
Ecl136II GAGCTC 1 cut(s) 144
Eco24I GRGCYC 2 cut(s) 83, 146
Eco47I GGWCC 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 144
EcoT38I GRGCYC 2 cut(s) 83, 146
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 4 cut(s) 125, 137, 202, 476
FaiI YATR 7 cut(s) 123, 135, 200, 339, 446, 474, 544
FatI CATG 4 cut(s) 121, 133, 198, 472
Fnu4HI GCNGC 3 cut(s) 239, 302, 344
FriOI GRGCYC 2 cut(s) 83, 146
Fsp4HI GCNGC 3 cut(s) 239, 302, 344
FspBI CTAG 1 cut(s) 84
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 239, 302, 344
HaeIII GGCC 2 cut(s) 5, 465
HhaI GCGC 1 cut(s) 118
Hin1II CATG 4 cut(s) 125, 137, 202, 476
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 2 cut(s) 31, 169
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 11
Hpy99I CGWCG 2 cut(s) 329, 364
HpyAV CCTTC 6 cut(s) 103, 343, 364, 443, 447, 500
HpyCH4III ACNGT 2 cut(s) 50, 162
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 271, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 382
HpyF3I CTNAG 5 cut(s) 28, 181, 225, 290, 420
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 4 cut(s) 125, 137, 202, 476
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 1 cut(s) 313
LguI GCTCTTC 1 cut(s) 445
LmnI GCTCC 2 cut(s) 76, 141
LpnPI CCDG 5 cut(s) 19, 278, 365, 447, 476
Lsp1109I GCAGC 3 cut(s) 225, 288, 355
LweI GCATC 2 cut(s) 103, 258
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 184
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MboII GAAGA 5 cut(s) 156, 400, 432, 474, 477
MhlI GDGCHC 4 cut(s) 36, 73, 83, 146
MluCI AATT 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 500
MseI TTAA 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 261, 382
NdeII GATC 1 cut(s) 313
NlaIII CATG 4 cut(s) 125, 137, 202, 476
NlaIV GGNNCC 2 cut(s) 156, 409
NmuCI GTSAC 1 cut(s) 184
NsbI TGCGCA 1 cut(s) 117
NspI RCATGY 1 cut(s) 125
PciI ACATGT 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 445
PkrI GCNGC 3 cut(s) 240, 303, 345
PscI ACATGT 1 cut(s) 121
Psp124BI GAGCTC 1 cut(s) 146
PspN4I GGNNCC 2 cut(s) 156, 409
PspPI GGNCC 1 cut(s) 408
RsaI GTAC 3 cut(s) 23, 47, 280
RsaNI GTAC 3 cut(s) 22, 46, 279
SacI GAGCTC 1 cut(s) 146
SapI GCTCTTC 1 cut(s) 445
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 3 cut(s) 239, 302, 344
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 1 cut(s) 408
ScaI AGTACT 1 cut(s) 47
SduI GDGCHC 4 cut(s) 36, 73, 83, 146
SfaNI GCATC 2 cut(s) 103, 258
SinI GGWCC 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 1 cut(s) 87
SsiI CCGC 1 cut(s) 385
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 146
TaaI ACNGT 2 cut(s) 50, 162
TaiI ACGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 359
TasI AATT 1 cut(s) 87
TatI WGTACW 2 cut(s) 21, 45
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 2 cut(s) 165, 189
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 3 cut(s) 238, 301, 343
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 326, 519
TspRI CASTG 2 cut(s) 165, 189
VpaK11BI GGWCC 1 cut(s) 408
XapI RAATTY 1 cut(s) 87
XceI RCATGY 1 cut(s) 125
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.