Rorug02G0127400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006528
Physical Location & Seq
Forward (+)
10967963 .. 10969078
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0127400.1

Sequence Viewer

Length: 1116 bp
ATGGCGTCCCAGAACGACACCGCCACCGCCACCGCCAGATCCGGATCCGGATCCAACGGCGAGGAGGAGAGTCGCAAGGTGGCGCTGATCACCGGAATCACCGGCCAGGACGGCTCCTACCTGACGGAGTTCCTCCTCAACAAAGGCTACGACGTCCACGGCCTGATCCGACGCTCCTCCAACTTCAACACCCAGCGGATCAACCACATCTACATCGACCCCCACAACGCCCACAAGGCCCGCATGAAGCTCCACTACGCCGACCTCACCGACGCCTCCTCCCTCCGCCGCTGGCTCGACACCATCGCCCCCGACGAGGTCTACAACCTCGCCGCCCAGTCCCACGTCGCCGTTTCCTTCGAGATCCCTGACTACACCGCCGACGTCGTCGCCACCGGCGCCCTCCGCCTCCTCGAAGCCGTCCGCAACCACGTCGACGCCACCGGAAGGACCAATGTCAAGTACTACCAGGCCGGGTCCTCCGAGATGTTCGGGGCCACCCCGCCTCCGCAGTCGGAGACGACGCCGTTTCATCCCCGATCCCCTTACGCCGCCTCGAAATGCGCGGCGCACTGGTACACCGTGAACTACCGCGAGGCCTACGGGCTCTACGCCTGCAATGGGATTCTGTTTAACCACGAGTCGCCGAGGAGGGGAGAGAATTTCGTGACCCGGAAGATCACTCGGGCCGTGGGCCGGATCAAAATCGGGCTGCAGAGCAAGCTGTTCTTGGGGAATTTGAATGCTTCAAGGGATTGGGGATTTGCTGGTGACTATGTGGAGGCAATGTGGATGATGCTACAGCAGGAGAAGCCTGATGACTATGTTGTGGCCACCGAGGAGTCCCACACGGTGGAGGAGTTCTTGGAGGTGGCGTTTGGGTATGTGGGGATGAACTGGAAGGATCATGTTGTGCTTGACAAGAGGTACTTGAGGCCGTCGGAGGTTGACAATCTGAAAGGGGATGCAAGCAAGGCGAAGAAGATTCTCGGGTGGAAGCCCAAAGTTGGGTTTCAGCAGCTGGTGAAGATGATGGTGGATGAAGATGTCGAATTGGCAAAGAGGGAAAAGGTTCTTGTTGATGCTGGATACATGGATGCCCAGCAACAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

371

Amino Acids

41.61

Weight (kDa)

6.47

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 28 - 278 2.6e-73 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 29 - 345 7.2e-140 GDP-mannose 4,6 dehydratase
RmlD_sub_bind PF04321 29 - 191 9.5e-06 RmlD substrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 156, 387
AccB1I GGYRCC 1 cut(s) 398
AccB7I CCANNNNNTGG 1 cut(s) 853
AccI GTMKAC 2 cut(s) 321, 435
AccII CGCG 2 cut(s) 566, 594
AccIII TCCGGA 2 cut(s) 41, 47
AcoI YGGCCR 2 cut(s) 103, 831
AcsI RAATTY 2 cut(s) 661, 736
AcyI GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
AdeI CACNNNGTG 1 cut(s) 853
AfaI GTAC 3 cut(s) 464, 578, 929
AfiI CCNNNNNNNGG 8 cut(s) 316, 447, 621, 653, 696, 853, 1007, 1008
AgsI TTSAA 3 cut(s) 187, 742, 750
AjiI CACGTC 2 cut(s) 346, 433
AjnI CCWGG 2 cut(s) 105, 468
AluBI AGCT 3 cut(s) 250, 724, 1021
AluI AGCT 3 cut(s) 250, 724, 1021
Alw26I GTCTC 1 cut(s) 512
AlwNI CAGNNNCTG 1 cut(s) 1021
Ama87I CYCGRG 2 cut(s) 684, 989
Aor13HI TCCGGA 2 cut(s) 41, 47
ApeKI GCWGC 2 cut(s) 712, 1018
ApoI RAATTY 2 cut(s) 661, 736
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 4 cut(s) 85, 401, 566, 571
AspS9I GGNCC 6 cut(s) 238, 450, 477, 495, 687, 694
AsuC2I CCSGG 2 cut(s) 475, 673
AsuHPI GGTGA 5 cut(s) 82, 91, 259, 782, 1036
AvaI CYCGRG 2 cut(s) 684, 989
AvaII GGWCC 2 cut(s) 450, 477
BalI TGGCCA 1 cut(s) 833
BamHI GGATCC 2 cut(s) 44, 50
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 609
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 638
BbvI GCAGC 2 cut(s) 699, 1030
BccI CCATC 2 cut(s) 311, 1027
BceAI ACGGC 8 cut(s) 73, 127, 175, 335, 404, 511, 674, 922
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 2 cut(s) 107, 470
BciVI GTATCC 1 cut(s) 1082
BclI TGATCA 1 cut(s) 87
BcnI CCSGG 2 cut(s) 475, 673
BcoDI GTCTC 1 cut(s) 512
BfmI CTRYAG 2 cut(s) 713, 800
BfoI RGCGCY 2 cut(s) 86, 402
BfuI GTATCC 1 cut(s) 1082
BglI GCCNNNNNGGC 2 cut(s) 111, 236
BisI GCNGC 6 cut(s) 289, 333, 552, 567, 713, 1019
BlsI GCNGC 6 cut(s) 290, 334, 553, 568, 714, 1020
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 4 cut(s) 107, 470, 475, 673
Bme18I GGWCC 2 cut(s) 450, 477
BmeT110I CYCGRG 2 cut(s) 684, 989
BmgBI CACGTC 2 cut(s) 346, 433
BmgT120I GGNCC 6 cut(s) 238, 450, 477, 495, 687, 694
BmiI GGNNCC 6 cut(s) 46, 52, 115, 400, 478, 496
BmrFI CCNGG 4 cut(s) 107, 470, 475, 673
BmrI ACTGGG 1 cut(s) 331
BmsI GCATC 4 cut(s) 786, 955, 1072, 1087
BmuI ACTGGG 1 cut(s) 331
BoxI GACNNNNGTC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 952
BpuMI CCSGG 2 cut(s) 475, 673
BsaBI GATNNNNATC 3 cut(s) 43, 49, 704
BsaHI GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
BsaJI CCNNGG 4 cut(s) 157, 647, 690, 837
BsaWI WCCGGW 4 cut(s) 41, 47, 92, 443
BsaXI ACNNNNNCTCC 8 cut(s) 263, 293, 490, 520, 773, 803, 860, 890
Bsc4I CCNNNNNNNGG 8 cut(s) 316, 447, 621, 653, 696, 853, 1007, 1008
Bse118I RCCGGY 2 cut(s) 101, 395
Bse1I ACTGG 3 cut(s) 337, 578, 902
Bse3DI GCAATG 2 cut(s) 625, 792
Bse8I GATNNNNATC 3 cut(s) 43, 49, 704
BseAI TCCGGA 2 cut(s) 41, 47
BseBI CCWGG 2 cut(s) 107, 470
BseDI CCNNGG 4 cut(s) 157, 647, 690, 837
BseGI GGATG 6 cut(s) 532, 798, 897, 970, 1045, 1102
BseJI GATNNNNATC 3 cut(s) 43, 49, 704
BseLI CCNNNNNNNGG 8 cut(s) 316, 447, 621, 653, 696, 853, 1007, 1008
BseMI GCAATG 2 cut(s) 625, 792
BseNI ACTGG 3 cut(s) 337, 578, 902
BseRI GAGGAG 9 cut(s) 77, 80, 125, 166, 268, 401, 664, 854, 872
BseXI GCAGC 2 cut(s) 699, 1030
BseYI CCCAGC 2 cut(s) 192, 1101
Bsh1236I CGCG 2 cut(s) 566, 594
BshNI GGYRCC 1 cut(s) 398
BsiHKCI CYCGRG 2 cut(s) 684, 989
BsiSI CCGG 9 cut(s) 42, 48, 93, 102, 396, 444, 474, 673, 697
BslFI GGGAC 2 cut(s) 325, 829
BslI CCNNNNNNNGG 8 cut(s) 316, 447, 621, 653, 696, 853, 1007, 1008
BsmAI GTCTC 1 cut(s) 512
BsmBI CGTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 325, 829
BsmI GAATGC 1 cut(s) 748
BsoBI CYCGRG 2 cut(s) 684, 989
Bsp1286I GDGCHC 1 cut(s) 609
Bsp13I TCCGGA 2 cut(s) 41, 47
BspEI TCCGGA 2 cut(s) 41, 47
BspFNI CGCG 2 cut(s) 566, 594
BspLI GGNNCC 6 cut(s) 46, 52, 115, 400, 478, 496
BspMAI CTGCAG 1 cut(s) 717
BspT107I GGYRCC 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 625, 792
BsrFI RCCGGY 2 cut(s) 101, 395
BsrI ACTGG 3 cut(s) 337, 578, 902
BssAI RCCGGY 2 cut(s) 101, 395
BssECI CCNNGG 4 cut(s) 157, 647, 690, 837
BssNI GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
BssSI CACGAG 1 cut(s) 638
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 2 cut(s) 107, 470
Bst4CI ACNGT 2 cut(s) 583, 853
BstACI GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
BstC8I GCNNGC 5 cut(s) 241, 293, 616, 722, 970
BstDSI CCRYGG 2 cut(s) 157, 690
BstF5I GGATG 6 cut(s) 532, 798, 897, 970, 1045, 1102
BstFNI CGCG 2 cut(s) 566, 594
BstH2I RGCGCY 2 cut(s) 86, 402
BstHHI GCGC 4 cut(s) 85, 401, 566, 571
BstMAI GTCTC 1 cut(s) 512
BstMWI GCNNNNNNNGC 7 cut(s) 111, 236, 398, 405, 721, 811, 974
BstNI CCWGG 2 cut(s) 107, 470
BstPAI GACNNNNGTC 1 cut(s) 455
BstSCI CCNGG 4 cut(s) 105, 468, 473, 671
BstSFI CTRYAG 2 cut(s) 713, 800
BstUI CGCG 2 cut(s) 566, 594
BstV1I GCAGC 2 cut(s) 699, 1030
BstX2I RGATCY 4 cut(s) 38, 44, 50, 363
BstYI RGATCY 4 cut(s) 38, 44, 50, 363
BsuI GTATCC 1 cut(s) 1082
BtgI CCRYGG 2 cut(s) 157, 690
BtgZI GCGATG 1 cut(s) 289
BtrI CACGTC 2 cut(s) 346, 433
BtsCI GGATG 6 cut(s) 532, 798, 897, 970, 1045, 1102
BtsIMutI CAGTG 1 cut(s) 571
Cac8I GCNNGC 5 cut(s) 241, 293, 616, 722, 970
CaiI CAGNNNCTG 1 cut(s) 1021
CfoI GCGC 4 cut(s) 85, 401, 566, 571
Cfr10I RCCGGY 2 cut(s) 101, 395
Cfr13I GGNCC 6 cut(s) 238, 450, 477, 495, 687, 694
CseI GACGC 4 cut(s) 180, 281, 446, 532
Csp6I GTAC 3 cut(s) 463, 577, 928
CviAII CATG 3 cut(s) 244, 908, 1093
CviQI GTAC 3 cut(s) 463, 577, 928
DinI GGCGCC 1 cut(s) 400
DraIII CACNNNGTG 1 cut(s) 853
EaeI YGGCCR 2 cut(s) 103, 831
EciI GGCGGA 2 cut(s) 275, 395
Eco147I AGGCCT 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 450, 477
Eco88I CYCGRG 2 cut(s) 684, 989
EcoO109I RGGNCCY 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 105, 468
EcoT38I GRGCYC 1 cut(s) 609
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 3 cut(s) 247, 911, 1096
FaiI YATR 6 cut(s) 245, 777, 825, 885, 909, 1094
FalI AAGNNNNNCTT 4 cut(s) 713, 745, 914, 946
FaqI GGGAC 2 cut(s) 325, 829
FatI CATG 3 cut(s) 243, 907, 1092
FauI CCCGC 2 cut(s) 248, 510
FbaI TGATCA 1 cut(s) 87
FblI GTMKAC 2 cut(s) 321, 435
Fnu4HI GCNGC 6 cut(s) 289, 333, 552, 567, 713, 1019
FokI GGATG 6 cut(s) 519, 805, 904, 977, 1052, 1109
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 6 cut(s) 289, 333, 552, 567, 713, 1019
GlaI GCGC 4 cut(s) 84, 400, 565, 570
GluI GCNGC 6 cut(s) 289, 333, 552, 567, 713, 1019
GsaI CCCAGC 2 cut(s) 196, 1105
HaeII RGCGCY 2 cut(s) 86, 402
HapII CCGG 9 cut(s) 42, 48, 93, 102, 396, 444, 474, 673, 697
HgaI GACGC 4 cut(s) 180, 281, 446, 532
HhaI GCGC 4 cut(s) 85, 401, 566, 571
Hin1I GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
Hin1II CATG 3 cut(s) 247, 911, 1096
Hin6I GCGC 4 cut(s) 83, 399, 564, 569
HinP1I GCGC 4 cut(s) 83, 399, 564, 569
HincII GTYRAC 2 cut(s) 436, 949
HindII GTYRAC 2 cut(s) 436, 949
HinfI GANTC 6 cut(s) 70, 96, 625, 641, 842, 985
HpaII CCGG 9 cut(s) 42, 48, 93, 102, 396, 444, 474, 673, 697
HphI GGTGA 5 cut(s) 82, 91, 259, 782, 1036
Hpy166II GTNNAC 6 cut(s) 157, 322, 436, 579, 586, 949
Hpy188I TCNGA 5 cut(s) 170, 484, 517, 943, 957
Hpy188III TCNNGA 4 cut(s) 42, 48, 361, 667
Hpy8I GTNNAC 6 cut(s) 157, 322, 436, 579, 586, 949
HpyAV CCTTC 3 cut(s) 367, 441, 895
HpyCH4III ACNGT 2 cut(s) 583, 853
HpyCH4IV ACGT 4 cut(s) 153, 345, 384, 432
HpyCH4V TGCA 3 cut(s) 618, 715, 968
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 236, 398, 405, 721, 811, 974
HpySE526I ACGT 4 cut(s) 153, 345, 384, 432
Hsp92I GRCGYC 7 cut(s) 5, 153, 273, 384, 399, 438, 524
Hsp92II CATG 3 cut(s) 247, 911, 1096
HspAI GCGC 4 cut(s) 83, 399, 564, 569
KasI GGCGCC 1 cut(s) 398
Kpn2I TCCGGA 2 cut(s) 41, 47
Ksp22I TGATCA 1 cut(s) 87
LmnI GCTCC 3 cut(s) 119, 179, 255
Lsp1109I GCAGC 2 cut(s) 699, 1030
LweI GCATC 4 cut(s) 786, 955, 1072, 1087
MaeII ACGT 4 cut(s) 153, 345, 384, 432
MaeIII GTNAC 2 cut(s) 667, 770
MboII GAAGA 5 cut(s) 688, 991, 994, 1039, 1055
MflI RGATCY 4 cut(s) 38, 44, 50, 363
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 1 cut(s) 833
MluCI AATT 3 cut(s) 661, 736, 1052
MluNI TGGCCA 1 cut(s) 833
Mly113I GGCGCC 1 cut(s) 399
MlyI GAGTC 3 cut(s) 79, 650, 851
MmeI TCCRAC 5 cut(s) 78, 193, 204, 495, 921
Mox20I TGGCCA 1 cut(s) 833
MroI TCCGGA 2 cut(s) 41, 47
MroXI GAANNNNTTC 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 833
MseI TTAA 1 cut(s) 633
Msp20I TGGCCA 1 cut(s) 833
MspA1I CMGCKG 3 cut(s) 196, 291, 1021
MspI CCGG 9 cut(s) 42, 48, 93, 102, 396, 444, 474, 673, 697
MspR9I CCNGG 4 cut(s) 107, 470, 475, 673
MteI GCGCNGCGC 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 2 cut(s) 107, 470
MvnI CGCG 2 cut(s) 566, 594
MwoI GCNNNNNNNGC 7 cut(s) 111, 236, 398, 405, 721, 811, 974
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 2 cut(s) 475, 673
NlaIII CATG 3 cut(s) 247, 911, 1096
NlaIV GGNNCC 6 cut(s) 46, 52, 115, 400, 478, 496
NmeAIII GCCGAG 1 cut(s) 672
NmuCI GTSAC 2 cut(s) 667, 770
PceI AGGCCT 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 3 cut(s) 96, 625, 985
PflFI GACNNNGTC 2 cut(s) 317, 386
PflMI CCANNNNNTGG 1 cut(s) 853
PkrI GCNGC 6 cut(s) 290, 334, 553, 568, 714, 1020
PleI GAGTC 3 cut(s) 78, 649, 850
PluTI GGCGCC 1 cut(s) 402
PpsI GAGTC 3 cut(s) 78, 649, 850
PpuMI RGGWCCY 1 cut(s) 477
PshAI GACNNNNGTC 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 105, 468
PspFI CCCAGC 2 cut(s) 192, 1101
PspGI CCWGG 2 cut(s) 105, 468
PspN4I GGNNCC 6 cut(s) 46, 52, 115, 400, 478, 496
PspPI GGNCC 6 cut(s) 238, 450, 477, 495, 687, 694
PspPPI RGGWCCY 1 cut(s) 477
PstI CTGCAG 1 cut(s) 717
PstNI CAGNNNCTG 1 cut(s) 1021
PsuI RGATCY 4 cut(s) 38, 44, 50, 363
PsyI GACNNNGTC 2 cut(s) 317, 386
PvuII CAGCTG 1 cut(s) 1021
RsaI GTAC 3 cut(s) 464, 578, 929
RsaNI GTAC 3 cut(s) 463, 577, 928
SalI GTCGAC 1 cut(s) 434
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 6 cut(s) 289, 333, 552, 567, 713, 1019
Sau96I GGNCC 6 cut(s) 238, 450, 477, 495, 687, 694
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 3 cut(s) 79, 650, 851
ScrFI CCNGG 4 cut(s) 107, 470, 475, 673
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 4 cut(s) 786, 955, 1072, 1087
SfcI CTRYAG 2 cut(s) 713, 800
SfoI GGCGCC 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 395
SgrDI CGTCGACG 1 cut(s) 434
SinI GGWCC 2 cut(s) 450, 477
SmlI CTYRAG 1 cut(s) 931
SmoI CTYRAG 1 cut(s) 931
Sse9I AATT 3 cut(s) 661, 736, 1052
SseBI AGGCCT 1 cut(s) 599
SspDI GGCGCC 1 cut(s) 398
StuI AGGCCT 1 cut(s) 599
StyD4I CCNGG 4 cut(s) 105, 468, 473, 671
TaaI ACNGT 2 cut(s) 583, 853
TaiI ACGT 4 cut(s) 156, 348, 387, 435
TaqI TCGA 7 cut(s) 216, 297, 360, 414, 435, 557, 1050
TasI AATT 3 cut(s) 661, 736, 1052
TatI WGTACW 1 cut(s) 462
TauI GCSGC 4 cut(s) 291, 335, 554, 569
TfiI GAWTC 3 cut(s) 96, 625, 985
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 1 cut(s) 578
TseFI GTSAC 2 cut(s) 667, 770
TseI GCWGC 2 cut(s) 712, 1018
Tsp45I GTSAC 2 cut(s) 667, 770
TspDTI ATGAA 4 cut(s) 260, 521, 908, 1056
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 1 cut(s) 578
Tth111I GACNNNGTC 2 cut(s) 317, 386
Van91I CCANNNNNTGG 1 cut(s) 853
VpaK11BI GGWCC 2 cut(s) 450, 477
XapI RAATTY 2 cut(s) 661, 736
XmiI GTMKAC 2 cut(s) 321, 435
XmnI GAANNNNTTC 1 cut(s) 1071
ZraI GACGTC 2 cut(s) 154, 385
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.