AT3G60910

Thiopurine S-methyltransferase (TPMT)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002378
Physical Location & Seq
Forward (+)
22501544 .. 22503372
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60910.2

Sequence Viewer

Length: 759 bp
ATGCAGAGGGACGTGTCGAGCTGCAACACTTACAACTATGGAGACGCTCTCTATTGGGACGCTCGTTACGTGCAAGACGCGCTTTCTTTCGACTGGTACCAATGTTACTCTTCTCTCCGCCCTTTTGTCCGCAGCTTCGTCTCTACTTCTTCACGAGTCCTCATGGTTGGCTGTGGCAACTCCCTGATGTCAGAGGATATGGTGAAGGATGGGTATGAGGACATCATGAATGTTGATATCTCTTCAGTGGCTATTGAGATGATGCAAACCAAATATGCATCTGTTCCTCAGCTCAAATATATGCAAATGGATGTTAGAGATATGAGTTACTTTGAAGACGATTCCTTTGACACTATTATTGATAAAGGAACCCTTGATTCATTGATGTGTGGCAGTGATGCTCTTCTAAGTGCTTCGAGAATGTTGGGTGAAGTTAGCAGGCTGATTAAACCTGGAGGGACCTACTTTTTGATAACCTACGGTGATCCGAAAGTGAGAATGCCTCATTTGACTCGTTCTGCGTACAACTGGAAGATCTCCTTATACATTATACCAAGACCAGGATTTAAAAGGCCAGAAAGTTGCAGCTCGTCAGCAAAGTCTTGTATGGAAGCTATACCTATAACAAGTGAGGGAATGCTGCCACATGATTATGTTCTGGAGGATCCTGATTCCCATTTTATTTACATATGCAAGAAGAAGGATGAAGATGAAGAAGCTCAACTACCCTCATACCCATTAATGGAGGATGTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

252

Amino Acids

28.75

Weight (kDa)

4.64

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 5 - 94 7.6e-10 Methyltransferase domain
Methyltransf_25 PF13649 5 - 91 3.5e-07 Methyltransferase domain
Methyltransf_31 PF13847 12 - 100 2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccII CGCG 1 cut(s) 80
AciI CCGC 2 cut(s) 118, 130
AclWI GGATC 3 cut(s) 479, 659, 672
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 2 cut(s) 98, 524
AfiI CCNNNNNNNGG 2 cut(s) 560, 742
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 1 cut(s) 335
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 451, 559
AluBI AGCT 6 cut(s) 21, 135, 292, 588, 614, 719
AluI AGCT 6 cut(s) 21, 135, 292, 588, 614, 719
Alw26I GTCTC 2 cut(s) 36, 145
AlwI GGATC 3 cut(s) 479, 659, 672
AoxI GGCC 1 cut(s) 572
ApeKI GCWGC 4 cut(s) 21, 132, 585, 640
ArsI GACNNNNNNTTYG 4 cut(s) 329, 361, 451, 483
AseI ATTAAT 1 cut(s) 740
Asp718I GGTACC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 459
AsuHPI GGTGA 3 cut(s) 214, 440, 494
AvaII GGWCC 1 cut(s) 459
BaeI ACNNNNGTAYC 2 cut(s) 88, 121
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 96
BarI GAAGNNNNNNTAC 2 cut(s) 708, 740
BauI CACGAG 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 342
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 8, 144, 597, 627
BccI CCATC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 453, 561
BcoDI GTCTC 2 cut(s) 36, 145
BglII AGATCT 1 cut(s) 534
BisI GCNGC 4 cut(s) 22, 133, 586, 641
BlsI GCNGC 4 cut(s) 23, 134, 587, 642
Bme1390I CCNGG 2 cut(s) 453, 561
Bme18I GGWCC 1 cut(s) 459
BmgBI CACGTC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 459
BmiI GGNNCC 4 cut(s) 98, 370, 460, 666
BmrFI CCNGG 2 cut(s) 453, 561
BmsI GCATC 3 cut(s) 252, 287, 388
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 4 cut(s) 33, 65, 487, 519
BpmI CTGGAG 2 cut(s) 474, 680
Bpu10I CCTNAGC 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 70
Bsc4I CCNNNNNNNGG 2 cut(s) 560, 742
Bse1I ACTGG 2 cut(s) 98, 533
BseBI CCWGG 2 cut(s) 453, 561
BseGI GGATG 4 cut(s) 214, 316, 709, 754
BseLI CCNNNNNNNGG 2 cut(s) 560, 742
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 2 cut(s) 98, 533
BseXI GCAGC 4 cut(s) 8, 144, 597, 627
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 574
BshNI GGYRCC 1 cut(s) 96
BslFI GGGAC 3 cut(s) 23, 71, 472
BslI CCNNNNNNNGG 2 cut(s) 560, 742
BsmAI GTCTC 2 cut(s) 36, 145
BsmBI CGTCTC 2 cut(s) 36, 145
BsmFI GGGAC 3 cut(s) 23, 71, 472
BsmI GAATGC 2 cut(s) 504, 642
BsnI GGCC 1 cut(s) 574
Bsp143I GATC 3 cut(s) 484, 534, 664
BspACI CCGC 2 cut(s) 118, 130
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 80
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 4 cut(s) 98, 370, 460, 666
BspPI GGATC 3 cut(s) 479, 659, 672
BspQI GCTCTTC 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 96
BsrI ACTGG 2 cut(s) 98, 533
BssMI GATC 3 cut(s) 484, 534, 664
BssSI CACGAG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 453, 561
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 3 cut(s) 115, 247, 408
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 288, 407
BstF5I GGATG 4 cut(s) 214, 316, 709, 754
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 487, 537, 667
BstMAI GTCTC 2 cut(s) 36, 145
BstMBI GATC 3 cut(s) 484, 534, 664
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 2 cut(s) 453, 561
BstSCI CCNGG 2 cut(s) 451, 559
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 4 cut(s) 8, 144, 597, 627
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 2 cut(s) 534, 664
BstYI RGATCY 2 cut(s) 534, 664
BsuRI GGCC 1 cut(s) 574
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 4 cut(s) 214, 316, 709, 754
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 252, 400
Cac8I GCNNGC 1 cut(s) 440
CciI TCATGA 1 cut(s) 225
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 459
CseI GACGC 3 cut(s) 53, 68, 86
Csp6I GTAC 2 cut(s) 97, 523
CviAII CATG 3 cut(s) 163, 226, 647
CviQI GTAC 2 cut(s) 97, 523
DdeI CTNAG 2 cut(s) 288, 407
DpnI GATC 3 cut(s) 486, 536, 666
DpnII GATC 3 cut(s) 484, 534, 664
DraI TTTAAA 1 cut(s) 568
Eam1104I CTCTTC 3 cut(s) 115, 247, 408
EarI CTCTTC 3 cut(s) 115, 247, 408
EciI GGCGGA 1 cut(s) 107
Eco32I GATATC 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 2 cut(s) 451, 559
EcoRV GATATC 1 cut(s) 238
EcoT22I ATGCAT 1 cut(s) 280
Esp3I CGTCTC 2 cut(s) 36, 145
FaeI CATG 3 cut(s) 166, 229, 650
FalI AAGNNNNNCTT 6 cut(s) 66, 98, 357, 389, 524, 556
FaqI GGGAC 3 cut(s) 23, 71, 472
FatI CATG 3 cut(s) 162, 225, 646
FauNDI CATATG 1 cut(s) 689
Fnu4HI GCNGC 4 cut(s) 22, 133, 586, 641
FokI GGATG 3 cut(s) 221, 323, 716
Fsp4HI GCNGC 4 cut(s) 22, 133, 586, 641
GlaI GCGC 1 cut(s) 81
GluI GCNGC 4 cut(s) 22, 133, 586, 641
GsuI CTGGAG 2 cut(s) 474, 680
HaeIII GGCC 1 cut(s) 574
HgaI GACGC 3 cut(s) 53, 68, 86
HhaI GCGC 1 cut(s) 82
Hin1II CATG 3 cut(s) 166, 229, 650
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 5 cut(s) 156, 341, 377, 511, 671
HphI GGTGA 3 cut(s) 214, 440, 494
Hpy188I TCNGA 2 cut(s) 193, 489
Hpy188III TCNNGA 5 cut(s) 153, 226, 417, 659, 668
HpyAV CCTTC 2 cut(s) 199, 694
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 2 cut(s) 12, 69
HpyCH4V TGCA 8 cut(s) 4, 24, 73, 265, 278, 304, 585, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 288, 407
HpySE526I ACGT 2 cut(s) 12, 69
Hsp92II CATG 3 cut(s) 166, 229, 650
HspAI GCGC 1 cut(s) 80
KpnI GGTACC 1 cut(s) 100
Kzo9I GATC 3 cut(s) 484, 534, 664
LguI GCTCTTC 1 cut(s) 408
Lsp1109I GCAGC 4 cut(s) 8, 144, 597, 627
LweI GCATC 3 cut(s) 252, 287, 388
MaeII ACGT 2 cut(s) 12, 69
MaeIII GTNAC 3 cut(s) 65, 104, 326
MalI GATC 3 cut(s) 486, 536, 666
MboI GATC 3 cut(s) 484, 534, 664
MboII GAAGA 9 cut(s) 102, 141, 234, 347, 395, 544, 709, 719, 725
MflI RGATCY 2 cut(s) 534, 664
MlyI GAGTC 2 cut(s) 165, 505
Mph1103I ATGCAT 1 cut(s) 280
MseI TTAA 3 cut(s) 447, 567, 740
MslI CAYNNNNRTG 2 cut(s) 385, 651
MspR9I CCNGG 2 cut(s) 453, 561
Mva1269I GAATGC 2 cut(s) 504, 642
MvaI CCWGG 2 cut(s) 453, 561
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 79
NdeI CATATG 1 cut(s) 689
NdeII GATC 3 cut(s) 484, 534, 664
NlaIII CATG 3 cut(s) 166, 229, 650
NlaIV GGNNCC 4 cut(s) 98, 370, 460, 666
NsiI ATGCAT 1 cut(s) 280
PagI TCATGA 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 2 cut(s) 504, 642
PfeI GAWTC 3 cut(s) 341, 377, 671
PkrI GCNGC 4 cut(s) 23, 134, 587, 642
PleI GAGTC 2 cut(s) 164, 505
PpsI GAGTC 2 cut(s) 164, 505
Ppu21I YACGTR 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 740
Psp5II RGGWCCY 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 451, 559
PspGI CCWGG 2 cut(s) 451, 559
PspN4I GGNNCC 4 cut(s) 98, 370, 460, 666
PspPI GGNCC 1 cut(s) 459
PspPPI RGGWCCY 1 cut(s) 459
PsuI RGATCY 2 cut(s) 534, 664
RsaI GTAC 2 cut(s) 98, 524
RsaNI GTAC 2 cut(s) 97, 523
RseI CAYNNNNRTG 2 cut(s) 385, 651
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 447, 567, 740
SatI GCNGC 4 cut(s) 22, 133, 586, 641
Sau3AI GATC 3 cut(s) 484, 534, 664
Sau96I GGNCC 1 cut(s) 459
SchI GAGTC 2 cut(s) 165, 505
ScrFI CCNGG 2 cut(s) 453, 561
SfaNI GCATC 3 cut(s) 252, 287, 388
SinI GGWCC 1 cut(s) 459
SmiMI CAYNNNNRTG 2 cut(s) 385, 651
SsiI CCGC 2 cut(s) 118, 130
StyD4I CCNGG 2 cut(s) 451, 559
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 2 cut(s) 15, 72
TaqI TCGA 3 cut(s) 17, 90, 416
TfiI GAWTC 3 cut(s) 341, 377, 671
Tru1I TTAA 3 cut(s) 447, 567, 740
Tru9I TTAA 3 cut(s) 447, 567, 740
TscAI CASTG 2 cut(s) 252, 400
TseI GCWGC 4 cut(s) 21, 132, 585, 640
TspDTI ATGAA 4 cut(s) 242, 369, 720, 726
TspRI CASTG 2 cut(s) 252, 400
VpaK11BI GGWCC 1 cut(s) 459
VspI ATTAAT 1 cut(s) 740
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.