Rroxscaffold_4G00329520

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002378
Physical Location & Seq
Reverse (-)
62840208 .. 62843660
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329520.1

Sequence Viewer

Length: 759 bp
ATGATGAGGGACGTGTCGAGCTGCAACACGTACGATTACGGAGACGCCCAATACTGGGACGCCCGCTACGTGCAAGAAGGTGACTCCTTATTCGACTGGTACCAGCGCTACTCTGCTCTCCGCCCTTTCATCCGCAACTTTATTCCCCTCACCTCCTCTCCTGTTCTCGTCGTCGGCTCCGGCAATGCCGTTATGTCCGAGGACATGGCGAAAGATGGATATGAAGACATAATGAACATAGACATCTCATCAGTGGCTATCAACCTCATGAAGACCAGAAACAAGCACATCCCTCAGTTGAAATACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAATCCTTTGACGGTATTATTGATAAAGGAACTCTTGATTCATTGATGTGTGGCAGTGATGCTCCGATTAGCGCTGCCCGAATGCTAGGGGAAGTGAGTAGGCTTCTTAAACCTGGAGGGGTCTATATTTTGATAACTTATGGTGATCCTAAAGTTAGAATGCCTCATTTGAGCCGGCCAGTGTACGACTGGAATACTGTTCTGTATATCCAACCCAGACCAGGATTCCAGAGGCCTGAAGGCTGCAATTCCTCAAGGAAGTCATATTTGGAACCTGTTCCTATCACCGAGAATGGTTCCCTTCCAGCTGAGTTTGTTATGGAAGACCCAGATTCACACTTCATTTATGTGTGTAAAAAGAAGGATGCCAAGGAATTACTTTCACTATATAGATTGAGAATTGAAGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

252

Amino Acids

28.8

Weight (kDa)

5.19

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 54 - 163 9.9e-11 Methyltransferase domain
Methyltransf_25 PF13649 55 - 155 3.3e-11 Methyltransferase domain
Methyltransf_11 PF08241 57 - 158 3e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AciI CCGC 3 cut(s) 64, 121, 133
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 521
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 3 cut(s) 32, 101, 530
AfeI AGCGCT 2 cut(s) 107, 418
AfiI CCNNNNNNNGG 3 cut(s) 54, 55, 566
AflIII ACRYGT 2 cut(s) 12, 27
AgsI TTSAA 2 cut(s) 301, 749
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 457, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AluBI AGCT 3 cut(s) 21, 333, 653
AluI AGCT 3 cut(s) 21, 333, 653
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 1 cut(s) 485
Aor51HI AGCGCT 2 cut(s) 107, 418
AoxI GGCC 2 cut(s) 521, 578
ApeKI GCWGC 3 cut(s) 21, 419, 588
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 2 cut(s) 108, 419
AsuHPI GGTGA 4 cut(s) 92, 142, 500, 622
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 3 cut(s) 231, 278, 675
BbvI GCAGC 3 cut(s) 8, 406, 575
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 2 cut(s) 459, 567
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 1 cut(s) 431
BfoI RGCGCY 2 cut(s) 109, 420
BisI GCNGC 3 cut(s) 22, 420, 589
BlsI GCNGC 3 cut(s) 23, 421, 590
Bme1390I CCNGG 2 cut(s) 459, 567
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 4 cut(s) 101, 178, 618, 643
BmrFI CCNGG 2 cut(s) 459, 567
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 394, 700
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 3 cut(s) 231, 278, 675
BpmI CTGGAG 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 583
BsaAI YACGTR 2 cut(s) 30, 70
BsaHI GRCGYC 2 cut(s) 45, 60
BsaJI CCNNGG 2 cut(s) 198, 714
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 55, 566
Bse118I RCCGGY 1 cut(s) 519
Bse1I ACTGG 4 cut(s) 59, 101, 524, 539
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 459, 567
BseDI CCNNGG 2 cut(s) 198, 714
BseGI GGATG 4 cut(s) 129, 288, 322, 715
BseLI CCNNNNNNNGG 3 cut(s) 54, 55, 566
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 308, 645
BseNI ACTGG 4 cut(s) 59, 101, 524, 539
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 3 cut(s) 8, 406, 575
BshFI GGCC 2 cut(s) 523, 580
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 180, 520
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 3 cut(s) 54, 55, 566
BsmAI GTCTC 1 cut(s) 36
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 23, 71
BsmI GAATGC 2 cut(s) 432, 510
BsnI GGCC 2 cut(s) 523, 580
Bsp143I GATC 1 cut(s) 490
BspACI CCGC 3 cut(s) 64, 121, 133
BspANI GGCC 2 cut(s) 523, 580
BspCNI CTCAG 2 cut(s) 307, 646
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 4 cut(s) 101, 178, 618, 643
BspPI GGATC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 519
BsrI ACTGG 4 cut(s) 59, 101, 524, 539
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 2 cut(s) 198, 714
BssMI GATC 1 cut(s) 490
BssNI GRCGYC 2 cut(s) 45, 60
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 459, 567
Bst4CI ACNGT 2 cut(s) 359, 544
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 2 cut(s) 30, 70
BstC8I GCNNGC 2 cut(s) 64, 521
BstDEI CTNAG 2 cut(s) 294, 654
BstF5I GGATG 4 cut(s) 129, 288, 322, 715
BstH2I RGCGCY 2 cut(s) 109, 420
BstHHI GCGC 2 cut(s) 108, 419
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 1 cut(s) 490
BstNI CCWGG 2 cut(s) 459, 567
BstSCI CCNGG 2 cut(s) 457, 565
BstV1I GCAGC 3 cut(s) 8, 406, 575
BstV2I GAAGAC 3 cut(s) 231, 278, 675
BsuRI GGCC 2 cut(s) 523, 580
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 4 cut(s) 129, 288, 322, 715
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 258, 406, 531
Cac8I GCNNGC 2 cut(s) 64, 521
CciI TCATGA 1 cut(s) 267
CfoI GCGC 2 cut(s) 108, 419
Cfr10I RCCGGY 1 cut(s) 519
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 3 cut(s) 31, 100, 529
CviAII CATG 2 cut(s) 205, 268
CviQI GTAC 3 cut(s) 31, 100, 529
DdeI CTNAG 2 cut(s) 294, 654
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 521
EciI GGCGGA 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 580
Eco47III AGCGCT 2 cut(s) 107, 418
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 2 cut(s) 457, 565
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 2 cut(s) 208, 271
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 2 cut(s) 204, 267
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 3 cut(s) 22, 420, 589
FokI GGATG 4 cut(s) 116, 275, 329, 722
Fsp4HI GCNGC 3 cut(s) 22, 420, 589
FspBI CTAG 1 cut(s) 431
GlaI GCGC 2 cut(s) 107, 418
GluI GCNGC 3 cut(s) 22, 420, 589
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 2 cut(s) 109, 420
HaeIII GGCC 2 cut(s) 523, 580
HapII CCGG 2 cut(s) 180, 520
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 2 cut(s) 108, 419
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 2 cut(s) 208, 271
Hin6I GCGC 2 cut(s) 106, 417
HinP1I GCGC 2 cut(s) 106, 417
HinfI GANTC 5 cut(s) 83, 347, 383, 570, 677
HpaII CCGG 2 cut(s) 180, 520
HphI GGTGA 4 cut(s) 92, 142, 500, 622
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 2 cut(s) 199, 411
Hpy188III TCNNGA 3 cut(s) 268, 380, 574
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 4 cut(s) 71, 578, 656, 700
HpyCH4III ACNGT 2 cut(s) 359, 544
HpyCH4IV ACGT 3 cut(s) 12, 29, 69
HpyCH4V TGCA 3 cut(s) 24, 73, 591
HpyF3I CTNAG 2 cut(s) 294, 654
HpySE526I ACGT 3 cut(s) 12, 29, 69
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 2 cut(s) 208, 271
HspAI GCGC 2 cut(s) 106, 417
KpnI GGTACC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 182, 412
Lsp1109I GCAGC 3 cut(s) 8, 406, 575
LweI GCATC 2 cut(s) 394, 700
MaeI CTAG 1 cut(s) 431
MaeII ACGT 3 cut(s) 12, 29, 69
MaeIII GTNAC 1 cut(s) 80
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 4 cut(s) 236, 283, 328, 680
MluCI AATT 3 cut(s) 592, 719, 744
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 580
MroNI GCCGGC 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 391, 692
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 180, 520
MspR9I CCNGG 2 cut(s) 459, 567
Mva1269I GAATGC 2 cut(s) 432, 510
MvaI CCWGG 2 cut(s) 459, 567
NaeI GCCGGC 1 cut(s) 521
NdeII GATC 1 cut(s) 490
NgoMIV GCCGGC 1 cut(s) 519
NlaIII CATG 2 cut(s) 208, 271
NlaIV GGNNCC 4 cut(s) 101, 178, 618, 643
NmuCI GTSAC 1 cut(s) 80
PagI TCATGA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 2 cut(s) 432, 510
PdiI GCCGGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 4 cut(s) 347, 383, 570, 677
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 3 cut(s) 23, 421, 590
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Ppu21I YACGTR 2 cut(s) 30, 70
Psp6I CCWGG 2 cut(s) 457, 565
PspGI CCWGG 2 cut(s) 457, 565
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 4 cut(s) 101, 178, 618, 643
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 3 cut(s) 32, 101, 530
RsaNI GTAC 3 cut(s) 31, 100, 529
RseI CAYNNNNRTG 2 cut(s) 391, 692
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 3 cut(s) 22, 420, 589
Sau3AI GATC 1 cut(s) 490
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 459, 567
SfaNI GCATC 2 cut(s) 394, 700
SmiMI CAYNNNNRTG 2 cut(s) 391, 692
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 3 cut(s) 592, 719, 744
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 3 cut(s) 64, 121, 133
SspMI CTAG 1 cut(s) 431
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 457, 565
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 2 cut(s) 359, 544
TaiI ACGT 3 cut(s) 15, 32, 72
TaqI TCGA 2 cut(s) 17, 93
TasI AATT 3 cut(s) 592, 719, 744
TfiI GAWTC 4 cut(s) 347, 383, 570, 677
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 258, 406, 531
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 3 cut(s) 21, 419, 588
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 7 cut(s) 118, 237, 248, 284, 360, 375, 676
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 258, 406, 531
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.