pycom02g25360

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002378
Physical Location & Seq
Forward (+)
23332491 .. 23334219
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25360.5

Sequence Viewer

Length: 480 bp
ATGTTATTGCCAGCATCAGAGCAGCAGCAGGAAAAGGAGATGTTGAGGGACGAGTCGAGCTGCAACACCTACAACTATAGCGACGCCCTCTACTGGGACGCCCGCTACGTCCAAGAAGGTGGCTCCTTCAACTGGTACCAGCTCTACTCTTCCCTCCGCCCTTTCGTCCGCCACTTCATCCCCATCTCCTCTTCTGTTCTCATGGTCGGCTGCGGCAATGCCGTTATGTCCGAGGACATGGTCAAGGATGGATATGAAGACATAATGAACATTGACGTTTCATCAGTGGCTATCAACATGATGAGAAGCAAATATGAGCACATACCTCAGCTGAAATACATGCAAATGGATGTTAGAGATATGAGTTTCTTCCCAGATGATTCGTTTGACGGTGTCATTGATAAAGAACTTTGTGCGGATCTCTTGGGTTATGAGCGGTCAATTCAGATCCGTTCATGCAAAGCAATTCCCTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

160

Amino Acids

18.41

Weight (kDa)

4.51

Isoelectric Point (pI)

57.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 436
AciI CCGC 6 cut(s) 103, 157, 169, 213, 416, 436
AclWI GGATC 2 cut(s) 426, 442
AcyI GRCGYC 2 cut(s) 84, 99
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 3 cut(s) 93, 94, 132
AgsI TTSAA 1 cut(s) 130
AluBI AGCT 3 cut(s) 60, 142, 331
AluI AGCT 3 cut(s) 60, 142, 331
Alw21I GWGCWC 1 cut(s) 321
AlwI GGATC 2 cut(s) 426, 442
ApeKI GCWGC 4 cut(s) 22, 25, 60, 210
Asp718I GGTACC 1 cut(s) 135
BanI GGYRCC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 4 cut(s) 34, 37, 47, 197
BccI CCATC 2 cut(s) 191, 242
BceAI ACGGC 1 cut(s) 206
BfmI CTRYAG 1 cut(s) 76
BisI GCNGC 5 cut(s) 23, 26, 61, 211, 214
BlsI GCNGC 5 cut(s) 24, 27, 62, 212, 215
BmiI GGNNCC 2 cut(s) 124, 137
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 327
BsaHI GRCGYC 2 cut(s) 84, 99
BsaJI CCNNGG 2 cut(s) 231, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 94, 132
Bse1I ACTGG 2 cut(s) 98, 137
Bse3DI GCAATG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 231, 471
BseGI GGATG 3 cut(s) 177, 253, 355
BseLI CCNNNNNNNGG 3 cut(s) 93, 94, 132
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 98, 137
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 4 cut(s) 34, 37, 47, 197
BshNI GGYRCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 321
BslFI GGGAC 2 cut(s) 62, 110
BslI CCNNNNNNNGG 3 cut(s) 93, 94, 132
BsmFI GGGAC 2 cut(s) 62, 110
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 418, 447
BspACI CCGC 6 cut(s) 103, 157, 169, 213, 416, 436
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 124, 137
BspPI GGATC 2 cut(s) 426, 442
BspT107I GGYRCC 1 cut(s) 135
BsrBI CCGCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 2 cut(s) 98, 137
BssECI CCNNGG 2 cut(s) 231, 471
BssMI GATC 2 cut(s) 418, 447
BssNI GRCGYC 2 cut(s) 84, 99
BssT1I CCWWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 2 cut(s) 154, 196
BstACI GRCGYC 2 cut(s) 84, 99
BstC8I GCNNGC 2 cut(s) 12, 103
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 3 cut(s) 177, 253, 355
BstKTI GATC 2 cut(s) 421, 450
BstMBI GATC 2 cut(s) 418, 447
BstNSI RCATGY 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 34, 37, 47, 197
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 2 cut(s) 418, 447
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 2 cut(s) 418, 447
BtsCI GGATG 3 cut(s) 177, 253, 355
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 2 cut(s) 12, 103
CseI GACGC 2 cut(s) 92, 107
Csp6I GTAC 1 cut(s) 136
CviAII CATG 5 cut(s) 202, 238, 298, 340, 456
CviJI RGCY 6 cut(s) 60, 123, 142, 210, 290, 331
CviKI_1 RGCY 6 cut(s) 60, 123, 142, 210, 290, 331
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 327
DpnI GATC 2 cut(s) 420, 449
DpnII GATC 2 cut(s) 418, 447
Eam1104I CTCTTC 2 cut(s) 154, 196
EarI CTCTTC 2 cut(s) 154, 196
EciI GGCGGA 2 cut(s) 146, 158
Eco130I CCWWGG 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 205, 241, 301, 343, 459
FaqI GGGAC 2 cut(s) 62, 110
FatI CATG 5 cut(s) 201, 237, 297, 339, 455
FauI CCCGC 1 cut(s) 110
Fnu4HI GCNGC 5 cut(s) 23, 26, 61, 211, 214
FokI GGATG 3 cut(s) 164, 260, 362
Fsp4HI GCNGC 5 cut(s) 23, 26, 61, 211, 214
GluI GCNGC 5 cut(s) 23, 26, 61, 211, 214
HgaI GACGC 2 cut(s) 92, 107
Hin1I GRCGYC 2 cut(s) 84, 99
Hin1II CATG 5 cut(s) 205, 241, 301, 343, 459
HinfI GANTC 2 cut(s) 53, 380
Hpy188I TCNGA 3 cut(s) 19, 232, 447
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 110, 136
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 2 cut(s) 108, 276
HpyCH4V TGCA 3 cut(s) 63, 343, 459
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 2 cut(s) 108, 276
Hsp92I GRCGYC 2 cut(s) 84, 99
Hsp92II CATG 5 cut(s) 205, 241, 301, 343, 459
KpnI GGTACC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 418, 447
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 6 cut(s) 14, 24, 79, 118, 152, 387
Lsp1109I GCAGC 4 cut(s) 34, 37, 47, 197
LweI GCATC 1 cut(s) 23
MaeII ACGT 2 cut(s) 108, 276
MalI GATC 2 cut(s) 420, 449
MbiI CCGCTC 1 cut(s) 436
MboI GATC 2 cut(s) 418, 447
MboII GAAGA 4 cut(s) 141, 183, 269, 361
MflI RGATCY 2 cut(s) 418, 447
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 2 cut(s) 441, 465
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 6 cut(s) 39, 98, 164, 199, 226, 336
MseI TTAA 1 cut(s) 478
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 331
NdeII GATC 2 cut(s) 418, 447
NlaIII CATG 5 cut(s) 205, 241, 301, 343, 459
NlaIV GGNNCC 2 cut(s) 124, 137
NspI RCATGY 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 1 cut(s) 380
PflFI GACNNNGTC 2 cut(s) 239, 392
PkrI GCNGC 5 cut(s) 24, 27, 62, 212, 215
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 124, 137
PsuI RGATCY 2 cut(s) 418, 447
PsyI GACNNNGTC 2 cut(s) 239, 392
PvuII CAGCTG 1 cut(s) 331
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 478
SatI GCNGC 5 cut(s) 23, 26, 61, 211, 214
Sau3AI GATC 2 cut(s) 418, 447
SchI GAGTC 1 cut(s) 62
SduI GDGCHC 1 cut(s) 321
SetI ASST 8 cut(s) 62, 71, 111, 121, 144, 279, 328, 333
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 2 cut(s) 441, 465
SsiI CCGC 6 cut(s) 103, 157, 169, 213, 416, 436
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 2 cut(s) 111, 279
TaqI TCGA 1 cut(s) 56
TasI AATT 2 cut(s) 441, 465
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 1 cut(s) 478
Tru9I TTAA 1 cut(s) 478
TscAI CASTG 1 cut(s) 291
TseI GCWGC 4 cut(s) 22, 25, 60, 210
TspDTI ATGAA 5 cut(s) 166, 270, 270, 281, 444
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 291
Tth111I GACNNNGTC 2 cut(s) 239, 392
XceI RCATGY 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.