Rh1BG027000

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002378
Physical Location & Seq
Forward (+)
3799764 .. 3802619
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG027000.1

Sequence Viewer

Length: 780 bp
ATGATGAGGGACGTGTCGACCTGCAACACGTACGATTACGGAGACGCCCAATACTGGGACGCCCGGTACGTCCAAGAAGGTGACTCCTTATTCGACTGGTACCAGCGCTACTCTGCTCTCCGCCCTTTCATCCGCAACTTCATTCCCCTCACCTCCTCTCCTGTTCTCGTCGCCGGCTCCGGCAATGCCGTTATGTCCGAGGACATGGTGAAAGATGGATATGAAGACATAATGAACATTGACATCTCATCAGTGGCTATCAACCTCATGAAGACCAGAAACAAGCACATCCCTCAGTTGAAATACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAATCCTTTGACGGTATTATTGATAAAGGAACTCTTGATTCATTGATGTGTGGCAGTGATGCTCCGATTAGTGCTGCCCGAATGCTAGGGGAAGTGAGTAGGCTTCTTAAACCTGGAGGGGTCTATATTTTGATAACTTATGGTGATCCTAAAGTTAGAATGCCTCATTTGAGCCGGCCAGTGTACGACTGGAATACTGTTCTGTATATCCAACGTAAGTGGAATTGTATGTCCTGTCATTTCAATTCCTCTTTTTCTAGCTGCTCAAATATGTTTAGTTTTATTTGTTCAAATTTCTATAAGTGGTATTGTTTCCGTATTGTGATTCAATTATATTTACTAATTTATGGTGAATTTAGTTATTGCATCTCACATTGTTTTCTTCTTTTGGTAGCAACTGTTTATATTTTGTTTGTTTCTATCTGTATGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

259

Amino Acids

29.97

Weight (kDa)

6.41

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 55 - 155 5.3e-10 Methyltransferase domain
Methyltransf_31 PF13847 55 - 168 1.2e-09 Methyltransferase domain
Methyltransf_11 PF08241 58 - 158 3.7e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 29
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 17
AciI CCGC 2 cut(s) 121, 133
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 521
AcsI RAATTY 2 cut(s) 637, 698
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 4 cut(s) 32, 68, 101, 530
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 54, 55
AflIII ACRYGT 2 cut(s) 12, 27
AgsI TTSAA 4 cut(s) 301, 589, 636, 674
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 2 cut(s) 333, 606
AluI AGCT 2 cut(s) 333, 606
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 1 cut(s) 485
Aor51HI AGCGCT 1 cut(s) 107
AoxI GGCC 1 cut(s) 521
ApeKI GCWGC 2 cut(s) 419, 606
ApoI RAATTY 2 cut(s) 637, 698
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 5 cut(s) 92, 142, 220, 500, 707
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 231, 278
BbvI GCAGC 2 cut(s) 406, 593
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 459
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 2 cut(s) 431, 603
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 29
BisI GCNGC 2 cut(s) 420, 607
BlsI GCNGC 2 cut(s) 421, 608
Bme1390I CCNGG 2 cut(s) 64, 459
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 101, 178
BmrFI CCNGG 2 cut(s) 64, 459
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 394, 720
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 2 cut(s) 231, 278
BpmI CTGGAG 1 cut(s) 480
BpuMI CCSGG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 2 cut(s) 45, 60
BsaJI CCNNGG 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 55
Bse118I RCCGGY 2 cut(s) 173, 519
Bse1I ACTGG 4 cut(s) 59, 101, 524, 539
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 3 cut(s) 129, 288, 322
BseLI CCNNNNNNNGG 2 cut(s) 54, 55
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 4 cut(s) 59, 101, 524, 539
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 406, 593
BshFI GGCC 1 cut(s) 523
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 4 cut(s) 64, 174, 180, 520
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 2 cut(s) 54, 55
BsmAI GTCTC 1 cut(s) 36
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 23, 71
BsmI GAATGC 2 cut(s) 432, 510
BsnI GGCC 1 cut(s) 523
Bsp143I GATC 1 cut(s) 490
BspACI CCGC 2 cut(s) 121, 133
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 1 cut(s) 307
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 101, 178
BspMI ACCTGC 1 cut(s) 29
BspPI GGATC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 173, 519
BsrI ACTGG 4 cut(s) 59, 101, 524, 539
BssAI RCCGGY 2 cut(s) 173, 519
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 1 cut(s) 490
BssNI GRCGYC 2 cut(s) 45, 60
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 3 cut(s) 359, 544, 745
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 175, 521
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 3 cut(s) 129, 288, 322
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 1 cut(s) 490
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 62, 457
BstV1I GCAGC 2 cut(s) 406, 593
BstV2I GAAGAC 2 cut(s) 231, 278
BsuRI GGCC 1 cut(s) 523
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 3 cut(s) 129, 288, 322
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 258, 406, 531
BveI ACCTGC 1 cut(s) 29
Cac8I GCNNGC 2 cut(s) 175, 521
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 2 cut(s) 173, 519
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 4 cut(s) 31, 67, 100, 529
CspCI CAANNNNNGTGG 2 cut(s) 545, 580
CviAII CATG 2 cut(s) 205, 268
CviJI RGCY 7 cut(s) 177, 257, 333, 448, 519, 523, 606
CviKI_1 RGCY 7 cut(s) 177, 257, 333, 448, 519, 523, 606
CviQI GTAC 4 cut(s) 31, 67, 100, 529
DdeI CTNAG 1 cut(s) 294
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 521
EciI GGCGGA 1 cut(s) 110
Eco47III AGCGCT 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 2 cut(s) 208, 271
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 2 cut(s) 204, 267
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 420, 607
FokI GGATG 3 cut(s) 116, 275, 329
Fsp4HI GCNGC 2 cut(s) 420, 607
FspBI CTAG 2 cut(s) 431, 603
GlaI GCGC 1 cut(s) 107
GluI GCNGC 2 cut(s) 420, 607
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 523
HapII CCGG 4 cut(s) 64, 174, 180, 520
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 2 cut(s) 208, 271
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 4 cut(s) 83, 347, 383, 670
HpaII CCGG 4 cut(s) 64, 174, 180, 520
HphI GGTGA 5 cut(s) 92, 142, 220, 500, 707
Hpy166II GTNNAC 2 cut(s) 18, 529
Hpy188I TCNGA 2 cut(s) 199, 411
Hpy188III TCNNGA 2 cut(s) 268, 380
Hpy8I GTNNAC 2 cut(s) 18, 529
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 3 cut(s) 359, 544, 745
HpyCH4IV ACGT 4 cut(s) 12, 29, 69, 559
HpyCH4V TGCA 2 cut(s) 24, 711
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 4 cut(s) 12, 29, 69, 559
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 2 cut(s) 208, 271
HspAI GCGC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 103
KroI GCCGGC 2 cut(s) 173, 519
KroNI GCCGGC 2 cut(s) 175, 521
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 182, 412
Lsp1109I GCAGC 2 cut(s) 406, 593
LweI GCATC 2 cut(s) 394, 720
MaeI CTAG 2 cut(s) 431, 603
MaeII ACGT 4 cut(s) 12, 29, 69, 559
MaeIII GTNAC 1 cut(s) 80
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 4 cut(s) 236, 283, 328, 719
MluCI AATT 6 cut(s) 568, 589, 637, 674, 687, 698
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 580
MroNI GCCGGC 2 cut(s) 173, 519
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 391
MspI CCGG 4 cut(s) 64, 174, 180, 520
MspR9I CCNGG 2 cut(s) 64, 459
Mva1269I GAATGC 2 cut(s) 432, 510
MvaI CCWGG 1 cut(s) 459
NaeI GCCGGC 2 cut(s) 175, 521
NciI CCSGG 1 cut(s) 64
NdeII GATC 1 cut(s) 490
NgoMIV GCCGGC 2 cut(s) 173, 519
NlaIII CATG 2 cut(s) 208, 271
NlaIV GGNNCC 2 cut(s) 101, 178
NmuCI GTSAC 1 cut(s) 80
PagI TCATGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 2 cut(s) 432, 510
PdiI GCCGGC 2 cut(s) 175, 521
PfeI GAWTC 3 cut(s) 347, 383, 670
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 2 cut(s) 421, 608
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Ppu21I YACGTR 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 101, 178
RsaI GTAC 4 cut(s) 32, 68, 101, 530
RsaNI GTAC 4 cut(s) 31, 67, 100, 529
RseI CAYNNNNRTG 1 cut(s) 391
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 420, 607
Sau3AI GATC 1 cut(s) 490
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 64, 459
SfaNI GCATC 2 cut(s) 394, 720
SmiMI CAYNNNNRTG 1 cut(s) 391
Sse9I AATT 6 cut(s) 568, 589, 637, 674, 687, 698
SsiI CCGC 2 cut(s) 121, 133
SspMI CTAG 2 cut(s) 431, 603
StyD4I CCNGG 2 cut(s) 62, 457
TaaI ACNGT 3 cut(s) 359, 544, 745
TaiI ACGT 4 cut(s) 15, 32, 72, 562
TaqI TCGA 2 cut(s) 17, 93
TasI AATT 6 cut(s) 568, 589, 637, 674, 687, 698
TfiI GAWTC 3 cut(s) 347, 383, 670
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 258, 406, 531
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 419, 606
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 7 cut(s) 118, 130, 237, 248, 284, 360, 375
TspGWI ACGGA 2 cut(s) 54, 650
TspRI CASTG 3 cut(s) 258, 406, 531
XapI RAATTY 2 cut(s) 637, 698
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 2 cut(s) 431, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.