Rw1G002430

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002378
Physical Location & Seq
Forward (+)
4455199 .. 4457699
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002430.1

Sequence Viewer

Length: 537 bp
ATGATGAAGGACGTGTCGAGCTGCAACACGTACGATTACGGAGACGCCCAATACTGGGACGCCCGCTACGTCCAAGAAGGTGACTCCTTATTCGACTGGTACCAGCGCTACTCTGCTCTCCGCCCTTTCATCCGCAACTTCATTCCCCTCGCCTCCTCTCCTGTTCTCGTCGTCGGCTCCGGCAATGCCGTTATGTCGGAGGACATGGCGAAAGATGGTTATGAAGACATAATGAACATTGACATCTCATCAGTGGCTATCAACCTCATGAAGACCAGAAACAAGCACATTCCTCAGTTGAAAGACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAATCCTTTGACGGTATTATTGATAAAGGTTACTCTGAGATGCATGACTCCTACGTTTTGGATTCAATTTCAATTGGCTCAAACAGTGATGGTAGTACTAATCTTGGTCATGATGAAAAACATACTGAGAAGGAAATAGATGGACGATTAAAGACACTGTTAAAGGCAATGTGGTTTCTAAAGCTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

178

Amino Acids

20.36

Weight (kDa)

4.75

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 55 - 123 2.4e-07 Methyltransferase domain
Methyltransf_11 PF08241 56 - 122 8.7e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AciI CCGC 3 cut(s) 64, 121, 133
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 3 cut(s) 32, 101, 442
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 54, 55
AflIII ACRYGT 2 cut(s) 12, 27
AgsI TTSAA 3 cut(s) 301, 411, 417
AjiI CACGTC 1 cut(s) 13
AluBI AGCT 3 cut(s) 21, 333, 529
AluI AGCT 3 cut(s) 21, 333, 529
Alw26I GTCTC 1 cut(s) 36
Aor51HI AGCGCT 1 cut(s) 107
ApeKI GCWGC 2 cut(s) 21, 529
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 231, 278
BbvI GCAGC 2 cut(s) 8, 516
BccI CCATC 3 cut(s) 209, 428, 479
BceAI ACGGC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 2 cut(s) 22, 530
BlsI GCNGC 2 cut(s) 23, 531
BmcAI AGTACT 1 cut(s) 442
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 101, 178
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 1 cut(s) 375
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 2 cut(s) 231, 278
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 2 cut(s) 45, 60
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 55
Bse1I ACTGG 2 cut(s) 59, 101
Bse3DI GCAATG 2 cut(s) 190, 519
BseGI GGATG 2 cut(s) 129, 322
BseLI CCNNNNNNNGG 2 cut(s) 54, 55
BseMI GCAATG 2 cut(s) 190, 519
BseMII CTCAG 3 cut(s) 308, 372, 462
BseNI ACTGG 2 cut(s) 59, 101
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 8, 516
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 180
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 54, 55
BsmAI GTCTC 1 cut(s) 36
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 71
BspACI CCGC 3 cut(s) 64, 121, 133
BspCNI CTCAG 3 cut(s) 307, 373, 463
BspHI TCATGA 2 cut(s) 267, 454
BspLI GGNNCC 2 cut(s) 101, 178
BspMAI CTGCAG 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 2 cut(s) 190, 519
BsrI ACTGG 2 cut(s) 59, 101
BssNI GRCGYC 2 cut(s) 45, 60
Bst4CI ACNGT 3 cut(s) 359, 431, 504
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 3 cut(s) 294, 381, 471
BstF5I GGATG 2 cut(s) 129, 322
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 530
BstV1I GCAGC 2 cut(s) 8, 516
BstV2I GAAGAC 2 cut(s) 231, 278
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 2 cut(s) 129, 322
BtsIMutI CAGTG 3 cut(s) 258, 436, 500
Cac8I GCNNGC 1 cut(s) 64
CciI TCATGA 2 cut(s) 267, 454
CfoI GCGC 1 cut(s) 108
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 3 cut(s) 31, 100, 441
CviAII CATG 4 cut(s) 205, 268, 389, 455
CviJI RGCY 6 cut(s) 21, 177, 257, 333, 423, 529
CviKI_1 RGCY 6 cut(s) 21, 177, 257, 333, 423, 529
CviQI GTAC 3 cut(s) 31, 100, 441
DdeI CTNAG 3 cut(s) 294, 381, 471
EciI GGCGGA 1 cut(s) 110
Eco47III AGCGCT 1 cut(s) 107
EcoT22I ATGCAT 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 4 cut(s) 208, 271, 392, 458
FaiI YATR 9 cut(s) 194, 206, 222, 230, 269, 329, 390, 456, 468
FaqI GGGAC 1 cut(s) 71
FatI CATG 4 cut(s) 204, 267, 388, 454
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 22, 530
FokI GGATG 2 cut(s) 116, 329
Fsp4HI GCNGC 2 cut(s) 22, 530
GlaI GCGC 1 cut(s) 107
GluI GCNGC 2 cut(s) 22, 530
HaeII RGCGCY 1 cut(s) 109
HapII CCGG 1 cut(s) 180
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 4 cut(s) 208, 271, 392, 458
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HinfI GANTC 4 cut(s) 83, 347, 392, 407
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 199, 382
Hpy188III TCNNGA 2 cut(s) 268, 455
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 2 cut(s) 71, 469
HpyCH4III ACNGT 3 cut(s) 359, 431, 504
HpyCH4IV ACGT 4 cut(s) 12, 29, 69, 399
HpyCH4V TGCA 3 cut(s) 24, 388, 532
HpyF3I CTNAG 3 cut(s) 294, 381, 471
HpySE526I ACGT 4 cut(s) 12, 29, 69, 399
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 4 cut(s) 208, 271, 392, 458
HspAI GCGC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 7 cut(s) 40, 82, 116, 174, 193, 289, 354
Lsp1109I GCAGC 2 cut(s) 8, 516
LweI GCATC 1 cut(s) 375
MaeII ACGT 4 cut(s) 12, 29, 69, 399
MaeIII GTNAC 2 cut(s) 80, 374
MboII GAAGA 3 cut(s) 236, 283, 328
MfeI CAATTG 1 cut(s) 417
MluCI AATT 2 cut(s) 411, 417
MlyI GAGTC 2 cut(s) 77, 386
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 158, 163, 166, 193, 275, 303, 315
Mph1103I ATGCAT 1 cut(s) 390
MseI TTAA 2 cut(s) 494, 506
MspI CCGG 1 cut(s) 180
MunI CAATTG 1 cut(s) 417
NlaIII CATG 4 cut(s) 208, 271, 392, 458
NlaIV GGNNCC 2 cut(s) 101, 178
NmuCI GTSAC 1 cut(s) 80
NsiI ATGCAT 1 cut(s) 390
PagI TCATGA 2 cut(s) 267, 454
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PfeI GAWTC 2 cut(s) 347, 407
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 2 cut(s) 23, 531
PleI GAGTC 2 cut(s) 77, 386
PpsI GAGTC 2 cut(s) 77, 386
Ppu21I YACGTR 1 cut(s) 30
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 101, 178
PstI CTGCAG 1 cut(s) 534
RsaI GTAC 3 cut(s) 32, 101, 442
RsaNI GTAC 3 cut(s) 31, 100, 441
SaqAI TTAA 2 cut(s) 494, 506
SatI GCNGC 2 cut(s) 22, 530
ScaI AGTACT 1 cut(s) 442
SchI GAGTC 2 cut(s) 77, 386
SfaNI GCATC 1 cut(s) 375
SfcI CTRYAG 1 cut(s) 530
Sse9I AATT 2 cut(s) 411, 417
SsiI CCGC 3 cut(s) 64, 121, 133
TaaI ACNGT 3 cut(s) 359, 431, 504
TaiI ACGT 4 cut(s) 15, 32, 72, 402
TaqI TCGA 2 cut(s) 17, 93
TasI AATT 2 cut(s) 411, 417
TatI WGTACW 1 cut(s) 440
TfiI GAWTC 2 cut(s) 347, 407
Tru1I TTAA 2 cut(s) 494, 506
Tru9I TTAA 2 cut(s) 494, 506
TscAI CASTG 3 cut(s) 258, 436, 507
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 21, 529
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 8 cut(s) 20, 118, 130, 237, 248, 284, 360, 474
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 258, 436, 507
ZrmI AGTACT 1 cut(s) 442
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.