MD07G1022400.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002378
Physical Location & Seq
Forward (+)
1872547 .. 1875884
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1022400.v1.1.491

Sequence Viewer

Length: 765 bp
ATGTTGAGGGACGAGTCGACCTGCAATACCTACAACTATGGCGACGCCCTCTACTGGGACGCCCGCTACGTCCAAGAAGGTGGCGCCTTCGACTGGTACCAGCGCTACTCTTCTCTCCGCCCTTTCGTCCGCCACTTCATCCCCACCTCCTCTCCTGTTCTCATGGTCGGCTGCGGCAATGCCGTTATGTCCGAGGACATGGTCAAGGATGGATATGAAGACATAATGAACATTGACATTTCGTCAGTGGCTATCAACATGATGAAAAGAAAATATGAGCACATACCTCAGCTGAAATACATGCAAATGGATGTCAGAGATATGAGTTTCTTTCCAGATGAATCATTTGATGGTGTCATTGATAAAGGAACTCTTGATTCACTAATGTGTGGCAACGATGCTCCAATTAGTGCTGCTCAAATGCTCGGGGAAGTGAGCAGGCTTCTTAAACCCGGAGGGATCTATATGTTGATAACCTATGGTGATCCTACAGTAAGAATGCCTCATTTGAGCCGGCCAGTGTACAACTGGAAAGTCCGTTTGTATGTTATACCCAGACCAGGATTCCAAACGCCTGAAGGCTGTGGTTCGTCAACAAAGTCAGATTTGGAACCTGTTCCTATCACTGAGAAGGGCTTACTTCCGGCTGATTTTGTTTTGGAAGATCCAGATTGTCACTTCATATATGTGTGTAAAAAGGACGATGCGGAGCTAAGTAATCCACCAACTGATGGATTGAGTCGATTGACAGTTGATGGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

255

Amino Acids

28.66

Weight (kDa)

4.74

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 52 - 160 1.6e-10 Methyltransferase domain
Methyltransf_25 PF13649 53 - 153 7.8e-13 Methyltransferase domain
Methyltransf_11 PF08241 54 - 156 1.7e-14 Methyltransferase domain
Methyltransf_12 PF08242 55 - 154 6.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 29
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 2 cut(s) 83, 96
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 1 cut(s) 17
AciI CCGC 5 cut(s) 64, 118, 130, 174, 707
AclWI GGATC 3 cut(s) 467, 479, 659
AcoI YGGCCR 1 cut(s) 515
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 3 cut(s) 45, 60, 84
AfaI GTAC 2 cut(s) 98, 524
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 5 cut(s) 54, 55, 93, 560, 731
AhdI GACNNNNNGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 559
AleI CACNNNNGTG 1 cut(s) 385
AluBI AGCT 2 cut(s) 292, 712
AluI AGCT 2 cut(s) 292, 712
Alw21I GWGCWC 1 cut(s) 282
AlwI GGATC 3 cut(s) 467, 479, 659
Ama87I CYCGRG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 2 cut(s) 171, 413
Asp700I GAANNNNTTC 1 cut(s) 615
Asp718I GGTACC 1 cut(s) 96
AspLEI GCGC 2 cut(s) 86, 105
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 425
BanI GGYRCC 2 cut(s) 83, 96
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 158, 400
BccI CCATC 4 cut(s) 203, 344, 725, 749
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 561
BcnI CCSGG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 2 cut(s) 87, 106
BfuAI ACCTGC 1 cut(s) 29
BisI GCNGC 3 cut(s) 172, 175, 414
BlsI GCNGC 3 cut(s) 173, 176, 415
Bme1390I CCNGG 2 cut(s) 453, 561
BmeRI GACNNNNNGTC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 425
BmiI GGNNCC 3 cut(s) 85, 98, 612
BmrFI CCNGG 2 cut(s) 453, 561
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 388, 694
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 288
BpuMI CCSGG 1 cut(s) 453
BsaHI GRCGYC 3 cut(s) 45, 60, 84
BsaJI CCNNGG 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 55, 93, 560, 731
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 4 cut(s) 59, 98, 518, 533
Bse3DI GCAATG 1 cut(s) 184
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 3 cut(s) 138, 214, 316
BseLI CCNNNNNNNGG 5 cut(s) 54, 55, 93, 560, 731
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 302, 618
BseNI ACTGG 4 cut(s) 59, 98, 518, 533
BseRI GAGGAG 1 cut(s) 139
BseXI GCAGC 2 cut(s) 158, 400
BshFI GGCC 1 cut(s) 517
BshNI GGYRCC 2 cut(s) 83, 96
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 3 cut(s) 453, 514, 644
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 5 cut(s) 54, 55, 93, 560, 731
BsmFI GGGAC 2 cut(s) 23, 71
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 459, 484, 664
BspACI CCGC 5 cut(s) 64, 118, 130, 174, 707
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 2 cut(s) 301, 619
BspLI GGNNCC 3 cut(s) 85, 98, 612
BspMI ACCTGC 1 cut(s) 29
BspPI GGATC 3 cut(s) 467, 479, 659
BspT107I GGYRCC 2 cut(s) 83, 96
BsrDI GCAATG 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 513
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 4 cut(s) 59, 98, 518, 533
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 3 cut(s) 459, 484, 664
BssNI GRCGYC 3 cut(s) 45, 60, 84
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 2 cut(s) 493, 751
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 3 cut(s) 45, 60, 84
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 64, 440, 515
BstDEI CTNAG 3 cut(s) 288, 627, 713
BstF5I GGATG 3 cut(s) 138, 214, 316
BstH2I RGCGCY 2 cut(s) 87, 106
BstHHI GCGC 2 cut(s) 86, 105
BstKTI GATC 3 cut(s) 462, 487, 667
BstMBI GATC 3 cut(s) 459, 484, 664
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 451, 559
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 158, 400
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 2 cut(s) 459, 664
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 2 cut(s) 459, 664
BsuRI GGCC 1 cut(s) 517
BtsCI GGATG 3 cut(s) 138, 214, 316
BtsIMutI CAGTG 3 cut(s) 252, 525, 624
BveI ACCTGC 1 cut(s) 29
Cac8I GCNNGC 3 cut(s) 64, 440, 515
CfoI GCGC 2 cut(s) 86, 105
Cfr10I RCCGGY 1 cut(s) 513
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 2 cut(s) 97, 523
CviAII CATG 4 cut(s) 163, 199, 259, 301
CviQI GTAC 2 cut(s) 97, 523
DdeI CTNAG 3 cut(s) 288, 627, 713
DinI GGCGCC 1 cut(s) 85
DpnI GATC 3 cut(s) 461, 486, 666
DpnII GATC 3 cut(s) 459, 484, 664
DriI GACNNNNNGTC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 515
Eam1104I CTCTTC 1 cut(s) 115
Eam1105I GACNNNNNGTC 1 cut(s) 241
EarI CTCTTC 1 cut(s) 115
EciI GGCGGA 2 cut(s) 107, 119
Eco47III AGCGCT 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 559
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
FaeI CATG 4 cut(s) 166, 202, 262, 304
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 4 cut(s) 162, 198, 258, 300
FauI CCCGC 1 cut(s) 71
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 3 cut(s) 172, 175, 414
FokI GGATG 3 cut(s) 125, 221, 323
Fsp4HI GCNGC 3 cut(s) 172, 175, 414
GlaI GCGC 2 cut(s) 85, 104
GluI GCNGC 3 cut(s) 172, 175, 414
HaeII RGCGCY 2 cut(s) 87, 106
HaeIII GGCC 1 cut(s) 517
HapII CCGG 3 cut(s) 453, 514, 644
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 2 cut(s) 86, 105
Hin1I GRCGYC 3 cut(s) 45, 60, 84
Hin1II CATG 4 cut(s) 166, 202, 262, 304
Hin6I GCGC 2 cut(s) 84, 103
HinP1I GCGC 2 cut(s) 84, 103
HincII GTYRAC 2 cut(s) 18, 594
HindII GTYRAC 2 cut(s) 18, 594
HinfI GANTC 5 cut(s) 14, 341, 377, 564, 739
HpaII CCGG 3 cut(s) 453, 514, 644
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 3 cut(s) 18, 523, 594
Hpy188I TCNGA 3 cut(s) 193, 317, 604
Hpy188III TCNNGA 3 cut(s) 335, 374, 668
Hpy8I GTNNAC 3 cut(s) 18, 523, 594
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 4 cut(s) 71, 97, 572, 625
HpyCH4III ACNGT 2 cut(s) 493, 751
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 24, 304
HpyF3I CTNAG 3 cut(s) 288, 627, 713
HpySE526I ACGT 1 cut(s) 69
Hsp92I GRCGYC 3 cut(s) 45, 60, 84
Hsp92II CATG 4 cut(s) 166, 202, 262, 304
HspAI GCGC 2 cut(s) 84, 103
KasI GGCGCC 1 cut(s) 83
KpnI GGTACC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 513
KroNI GCCGGC 1 cut(s) 515
Kzo9I GATC 3 cut(s) 459, 484, 664
LmnI GCTCC 2 cut(s) 406, 709
Lsp1109I GCAGC 2 cut(s) 158, 400
LweI GCATC 2 cut(s) 388, 694
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 674
MalI GATC 3 cut(s) 461, 486, 666
MboI GATC 3 cut(s) 459, 484, 664
MboII GAAGA 3 cut(s) 102, 230, 674
MflI RGATCY 2 cut(s) 459, 664
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 1 cut(s) 405
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 23, 748
MnlI CCTC 7 cut(s) 59, 157, 160, 187, 297, 449, 513
MroNI GCCGGC 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 3 cut(s) 305, 385, 686
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 3 cut(s) 453, 514, 644
MspR9I CCNGG 2 cut(s) 453, 561
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 1 cut(s) 561
NaeI GCCGGC 1 cut(s) 515
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 1 cut(s) 453
NdeII GATC 3 cut(s) 459, 484, 664
NgoMIV GCCGGC 1 cut(s) 513
NlaIII CATG 4 cut(s) 166, 202, 262, 304
NlaIV GGNNCC 3 cut(s) 85, 98, 612
NmuCI GTSAC 1 cut(s) 674
NspI RCATGY 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 504
PdiI GCCGGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 3 cut(s) 341, 377, 564
PflFI GACNNNGTC 1 cut(s) 200
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 3 cut(s) 173, 176, 415
PleI GAGTC 2 cut(s) 22, 747
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 2 cut(s) 22, 747
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 85, 98, 612
PsuI RGATCY 2 cut(s) 459, 664
PsyI GACNNNGTC 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 2 cut(s) 98, 524
RsaNI GTAC 2 cut(s) 97, 523
RseI CAYNNNNRTG 3 cut(s) 305, 385, 686
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 3 cut(s) 172, 175, 414
Sau3AI GATC 3 cut(s) 459, 484, 664
SchI GAGTC 2 cut(s) 23, 748
ScrFI CCNGG 2 cut(s) 453, 561
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 2 cut(s) 388, 694
SfcI CTRYAG 1 cut(s) 489
SfoI GGCGCC 1 cut(s) 85
SmiMI CAYNNNNRTG 3 cut(s) 305, 385, 686
Sse9I AATT 1 cut(s) 405
SsiI CCGC 5 cut(s) 64, 118, 130, 174, 707
SspDI GGCGCC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 451, 559
TaaI ACNGT 2 cut(s) 493, 751
TaiI ACGT 1 cut(s) 72
TaqI TCGA 3 cut(s) 17, 90, 742
TasI AATT 1 cut(s) 405
TatI WGTACW 1 cut(s) 522
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 3 cut(s) 341, 377, 564
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 252, 525, 631
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 2 cut(s) 171, 413
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 6 cut(s) 127, 231, 242, 278, 354, 670
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 3 cut(s) 252, 525, 631
Tth111I GACNNNGTC 1 cut(s) 200
Van91I CCANNNNNTGG 1 cut(s) 731
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 525
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.