FvH4_7g01670

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002378
Physical Location & Seq
Forward (+)
2057170 .. 2060933
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01670.t4

Sequence Viewer

Length: 759 bp
ATGATGAGGGACGTATCGAGCTGCAACACGTACGATTACGGAGACGCCAATTACTGGGACGCCCGTTACGTCCAAGAAGGCGACTCCTTATTCGACTGGTACCAACGCTACTCTGCTCTCCGCCCTTTTATCCGCAACTTCATCCCCGACACCTCCTCCCCTGTTCTCGTCGTCGGCTGCGGCAATGCCGTGATGTCCGAGGACATGGCCAAGGATGGATATCAAGACATTATGAACATTGACATCTCTTCTGTGGCTATCAACCTCATGAAAACCCGAAACCAGCACATTCCTCAGTTGAAATACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAGTCCTTCGACGGTATCGTTGATAAAGGAACCCTTGATTCATTGATGTGTGGCAATGATGCTCCGATTAGTGCTGCCCAAATGCTAGGGGAAGTGAATAGGCTTCTCAAACCTGGAGGGGTCTATATTTTGATAACTTATGGTGACCCTAAAGTTAGAATGCCTCATTTGAGTCGGCCGGTGTACGATTGGAACACCGTTCTGTATATCCAACCCAGACCTGGATTCCAGAGGCCTGAAGGCTGCAGTTCCTCAAAAAAGTCATATTTGGAACCTGTTCCTGTCACTCAAAATGGATCCCTTCCAGCTGAATTTGTGATGGAAGATCCATATTCCCACTTCATATACGTGTGTAAAAAGAAGGATGCCACGGAATTACTTGCATTATATAGATTGAGACTTGATGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

253

Amino Acids

28.74

Weight (kDa)

5.02

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 34 - 166 8.1e-07 Methyltransferase domain
Methyltransf_31 PF13847 53 - 168 1.3e-12 Methyltransferase domain
Methyltransf_25 PF13649 55 - 155 5.1e-13 Methyltransferase domain
Methyltransf_11 PF08241 56 - 158 1.1e-14 Methyltransferase domain
Methyltransf_12 PF08242 56 - 156 1.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 54
AciI CCGC 3 cut(s) 121, 133, 180
AclWI GGATC 3 cut(s) 636, 649, 665
AcoI YGGCCR 2 cut(s) 207, 521
AcsI RAATTY 1 cut(s) 656
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 3 cut(s) 32, 101, 530
AfiI CCNNNNNNNGG 2 cut(s) 54, 566
AflIII ACRYGT 2 cut(s) 27, 693
AgsI TTSAA 1 cut(s) 301
AjnI CCWGG 2 cut(s) 457, 565
AluBI AGCT 3 cut(s) 21, 333, 653
AluI AGCT 3 cut(s) 21, 333, 653
Alw26I GTCTC 2 cut(s) 36, 736
AlwI GGATC 3 cut(s) 636, 649, 665
AoxI GGCC 3 cut(s) 207, 521, 578
ApeKI GCWGC 4 cut(s) 21, 177, 419, 588
ApoI RAATTY 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 500
BalI TGGCCA 1 cut(s) 209
BamHI GGATCC 1 cut(s) 641
BanI GGYRCC 1 cut(s) 99
BbvI GCAGC 4 cut(s) 8, 164, 406, 575
BccI CCATC 2 cut(s) 209, 658
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 2 cut(s) 459, 567
BcoDI GTCTC 2 cut(s) 36, 736
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 1 cut(s) 589
BisI GCNGC 5 cut(s) 22, 178, 181, 420, 589
BlsI GCNGC 5 cut(s) 23, 179, 182, 421, 590
Bme1390I CCNGG 2 cut(s) 459, 567
BmiI GGNNCC 4 cut(s) 101, 376, 618, 643
BmrFI CCNGG 2 cut(s) 459, 567
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 394, 700
BmuI ACTGGG 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 480
BsaAI YACGTR 2 cut(s) 30, 694
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 2 cut(s) 45, 60
BsaJI CCNNGG 3 cut(s) 198, 210, 714
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 566
Bse118I RCCGGY 1 cut(s) 523
Bse1I ACTGG 2 cut(s) 59, 101
Bse3DI GCAATG 2 cut(s) 190, 406
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 2 cut(s) 459, 567
BseDI CCNNGG 3 cut(s) 198, 210, 714
BseGI GGATG 4 cut(s) 141, 220, 322, 715
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 54, 566
BseMI GCAATG 2 cut(s) 190, 406
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 59, 101
BseRI GAGGAG 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 521
BseXI GCAGC 4 cut(s) 8, 164, 406, 575
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 3 cut(s) 209, 523, 580
BshNI GGYRCC 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 524
BsiSI CCGG 1 cut(s) 524
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 2 cut(s) 54, 566
BsmAI GTCTC 2 cut(s) 36, 736
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 23, 71
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 3 cut(s) 209, 523, 580
Bsp143I GATC 2 cut(s) 641, 670
BspACI CCGC 3 cut(s) 121, 133, 180
BspANI GGCC 3 cut(s) 209, 523, 580
BspCNI CTCAG 1 cut(s) 307
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 4 cut(s) 101, 376, 618, 643
BspMAI CTGCAG 1 cut(s) 593
BspPI GGATC 3 cut(s) 636, 649, 665
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 2 cut(s) 190, 406
BsrFI RCCGGY 1 cut(s) 523
BsrI ACTGG 2 cut(s) 59, 101
BssAI RCCGGY 1 cut(s) 523
BssECI CCNNGG 3 cut(s) 198, 210, 714
BssMI GATC 2 cut(s) 641, 670
BssNI GRCGYC 2 cut(s) 45, 60
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 459, 567
Bst4CI ACNGT 2 cut(s) 359, 544
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 2 cut(s) 30, 694
BstDEI CTNAG 1 cut(s) 294
BstDSI CCRYGG 1 cut(s) 714
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 4 cut(s) 141, 220, 322, 715
BstKTI GATC 2 cut(s) 644, 673
BstMAI GTCTC 2 cut(s) 36, 736
BstMBI GATC 2 cut(s) 641, 670
BstMCI CGRYCG 1 cut(s) 524
BstNI CCWGG 2 cut(s) 459, 567
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 2 cut(s) 457, 565
BstSFI CTRYAG 1 cut(s) 589
BstV1I GCAGC 4 cut(s) 8, 164, 406, 575
BstX2I RGATCY 2 cut(s) 641, 670
BstYI RGATCY 2 cut(s) 641, 670
BstZI CGGCCG 1 cut(s) 521
BsuRI GGCC 3 cut(s) 209, 523, 580
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 4 cut(s) 141, 220, 322, 715
CciI TCATGA 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 523
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 3 cut(s) 31, 100, 529
CviAII CATG 2 cut(s) 205, 268
CviQI GTAC 3 cut(s) 31, 100, 529
DdeI CTNAG 1 cut(s) 294
DpnI GATC 2 cut(s) 643, 672
DpnII GATC 2 cut(s) 641, 670
EaeI YGGCCR 2 cut(s) 207, 521
EagI CGGCCG 1 cut(s) 521
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 210
Eco147I AGGCCT 1 cut(s) 580
Eco32I GATATC 1 cut(s) 221
Eco52I CGGCCG 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 603
Eco91I GGTNACC 1 cut(s) 488
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 2 cut(s) 457, 565
EcoRV GATATC 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 2 cut(s) 208, 271
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 2 cut(s) 204, 267
Fnu4HI GCNGC 5 cut(s) 22, 178, 181, 420, 589
FokI GGATG 4 cut(s) 128, 227, 329, 722
Fsp4HI GCNGC 5 cut(s) 22, 178, 181, 420, 589
FspBI CTAG 1 cut(s) 431
GluI GCNGC 5 cut(s) 22, 178, 181, 420, 589
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 3 cut(s) 209, 523, 580
HapII CCGG 1 cut(s) 524
HgaI GACGC 2 cut(s) 53, 68
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 2 cut(s) 208, 271
HinfI GANTC 5 cut(s) 83, 347, 383, 517, 570
HpaII CCGG 1 cut(s) 524
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 2 cut(s) 199, 411
Hpy188III TCNNGA 3 cut(s) 224, 268, 574
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 3 cut(s) 173, 176, 359
HpyAV CCTTC 5 cut(s) 71, 361, 578, 656, 700
HpyCH4III ACNGT 2 cut(s) 359, 544
HpyCH4IV ACGT 4 cut(s) 12, 29, 69, 693
HpyCH4V TGCA 3 cut(s) 24, 591, 728
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 4 cut(s) 12, 29, 69, 693
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 2 cut(s) 208, 271
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 641, 670
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 4 cut(s) 8, 164, 406, 575
LweI GCATC 2 cut(s) 394, 700
MaeI CTAG 1 cut(s) 431
MaeII ACGT 4 cut(s) 12, 29, 69, 693
MaeIII GTNAC 3 cut(s) 65, 488, 628
MalI GATC 2 cut(s) 643, 672
MboI GATC 2 cut(s) 641, 670
MboII GAAGA 3 cut(s) 240, 328, 680
MflI RGATCY 2 cut(s) 641, 670
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 3 cut(s) 49, 656, 719
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 3 cut(s) 77, 356, 526
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 391, 692
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 459, 567
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 2 cut(s) 459, 567
NdeII GATC 2 cut(s) 641, 670
NlaIII CATG 2 cut(s) 208, 271
NlaIV GGNNCC 4 cut(s) 101, 376, 618, 643
NmuCI GTSAC 2 cut(s) 488, 628
PagI TCATGA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 360
PctI GAATGC 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 2 cut(s) 383, 570
Pfl23II CGTACG 1 cut(s) 30
PflMI CCANNNNNTGG 1 cut(s) 54
PkrI GCNGC 5 cut(s) 23, 179, 182, 421, 590
PleI GAGTC 3 cut(s) 77, 355, 525
PpsI GAGTC 3 cut(s) 77, 355, 525
Ppu21I YACGTR 2 cut(s) 30, 694
Psp6I CCWGG 2 cut(s) 457, 565
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 2 cut(s) 457, 565
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 4 cut(s) 101, 376, 618, 643
PstI CTGCAG 1 cut(s) 593
PsuI RGATCY 2 cut(s) 641, 670
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 3 cut(s) 32, 101, 530
RsaNI GTAC 3 cut(s) 31, 100, 529
RseI CAYNNNNRTG 2 cut(s) 391, 692
SatI GCNGC 5 cut(s) 22, 178, 181, 420, 589
Sau3AI GATC 2 cut(s) 641, 670
SchI GAGTC 3 cut(s) 77, 356, 526
ScrFI CCNGG 2 cut(s) 459, 567
SfaNI GCATC 2 cut(s) 394, 700
SfcI CTRYAG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 391, 692
Sse9I AATT 3 cut(s) 49, 656, 719
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 3 cut(s) 121, 133, 180
SspMI CTAG 1 cut(s) 431
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 457, 565
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 2 cut(s) 359, 544
TaiI ACGT 4 cut(s) 15, 32, 72, 696
TaqI TCGA 3 cut(s) 17, 93, 354
TasI AATT 3 cut(s) 49, 656, 719
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 2 cut(s) 383, 570
TseFI GTSAC 2 cut(s) 488, 628
TseI GCWGC 4 cut(s) 21, 177, 419, 588
Tsp45I GTSAC 2 cut(s) 488, 628
TspDTI ATGAA 5 cut(s) 130, 248, 284, 375, 676
TspGWI ACGGA 2 cut(s) 54, 731
Van91I CCANNNNNTGG 1 cut(s) 54
XapI RAATTY 1 cut(s) 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.