AT4G02270

pollen Ole e 1 allergen and extensin family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008322
Physical Location & Seq
Reverse (-)
992111 .. 993039
929 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02270.1

Sequence Viewer

Length: 498 bp
ATGGCTTTCTCACGCCTCTCATTTGCAGCTTCTCTCATCGTCTTCTCATCTCTAATCATATCTTCAGTTGCTTATTACGGCAATGAAGCTGACCCGGAGACCGGAAAATTGATTCCCATCGCCGTTGAAGGGATCATCAAGTGCAAGTCCGGTGGCAAAACTTACCCAATTCAAGGTGCAACGGCAAGAATTGCGTGTGTGAAGGTCGATGCATATGGGAATGAGTTAGTTCCAATATCGATATTGAGCAGCAAAACTGATGCTAAAGGTTACTTCATCGCCACGATATTCCCTTCGCAGCTTCGTGCAGGAAGGACAGTGACCAAATGCAAAACTTACCTTTACAAATCTCCACTCGCTGATTGCGATTTTCCGACCGATGTGAACAAAGGTGTTAGAGGACAGCCATTGAGCACGTACCGTATTCTTCAAGACAAGAGCTTCAAGCTTTACTGGGCTGGTCCTTTCTTCTACACGTCTGAACCTACTTACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

165

Amino Acids

18.1

Weight (kDa)

9.21

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 42 - 134 1.3e-15 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 101, 129, 173
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 4 cut(s) 128, 173, 431, 445
AjiI CACGTC 1 cut(s) 477
AluBI AGCT 5 cut(s) 29, 89, 301, 441, 448
AluI AGCT 5 cut(s) 29, 89, 301, 441, 448
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 140
ApeKI GCWGC 3 cut(s) 26, 249, 298
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 38, 261, 310
BccI CCATC 1 cut(s) 125
BceAI ACGGC 3 cut(s) 94, 107, 198
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 92
BisI GCNGC 3 cut(s) 27, 250, 299
BlsI GCNGC 3 cut(s) 28, 251, 300
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 1 cut(s) 461
BmrFI CCNGG 1 cut(s) 95
BmrI ACTGGG 1 cut(s) 463
BmsI GCATC 2 cut(s) 199, 250
BmuI ACTGGG 1 cut(s) 463
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 92
BsaWI WCCGGW 2 cut(s) 101, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 129, 173
Bse1I ACTGG 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 239
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 3 cut(s) 101, 129, 173
BseMI GCAATG 1 cut(s) 88
BseNI ACTGG 1 cut(s) 458
BseXI GCAGC 3 cut(s) 38, 261, 310
BsgI GTGCAG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 378
BshVI ATCGAT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 416
BsiSI CCGG 3 cut(s) 95, 102, 150
BslI CCNNNNNNNGG 3 cut(s) 101, 129, 173
BsmAI GTCTC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 1 cut(s) 132
BspDI ATCGAT 1 cut(s) 239
BspPI GGATC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 88
BsrI ACTGG 1 cut(s) 458
BssMI GATC 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 319, 422
BstAPI GCANNNNNTGC 1 cut(s) 191
BstBAI YACGTR 1 cut(s) 417
BstKTI GATC 1 cut(s) 135
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 1 cut(s) 132
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 38, 261, 310
BstV2I GAAGAC 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 239
BsuTUI ATCGAT 1 cut(s) 239
BtgZI GCGATG 2 cut(s) 103, 262
BtrI CACGTC 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 239
Csp6I GTAC 1 cut(s) 418
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviJI RGCY 8 cut(s) 5, 29, 89, 301, 406, 441, 448, 458
CviKI_1 RGCY 8 cut(s) 5, 29, 89, 301, 406, 441, 448, 458
CviQI GTAC 1 cut(s) 418
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 214
FaiI YATR 3 cut(s) 59, 214, 216
FauNDI CATATG 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 27, 250, 299
Fsp4HI GCNGC 3 cut(s) 27, 250, 299
GluI GCNGC 3 cut(s) 27, 250, 299
HapII CCGG 3 cut(s) 95, 102, 150
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 1 cut(s) 112
HpaII CCGG 3 cut(s) 95, 102, 150
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 375, 481
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 385
HpyAV CCTTC 4 cut(s) 122, 196, 303, 306
HpyCH4III ACNGT 2 cut(s) 319, 422
HpyCH4IV ACGT 2 cut(s) 416, 476
HpyCH4V TGCA 6 cut(s) 26, 144, 179, 212, 308, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpySE526I ACGT 2 cut(s) 416, 476
Kzo9I GATC 1 cut(s) 132
LpnPI CCDG 6 cut(s) 108, 115, 163, 294, 439, 444
Lsp1109I GCAGC 3 cut(s) 38, 261, 310
LweI GCATC 2 cut(s) 199, 250
MaeII ACGT 2 cut(s) 416, 476
MaeIII GTNAC 2 cut(s) 269, 319
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 4 cut(s) 34, 54, 419, 460
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 3 cut(s) 107, 168, 189
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 2 cut(s) 26, 392
Mph1103I ATGCAT 1 cut(s) 214
MspI CCGG 3 cut(s) 95, 102, 150
MspR9I CCNGG 1 cut(s) 95
MwoI GCNNNNNNNGC 1 cut(s) 191
NciI CCSGG 1 cut(s) 95
NdeI CATATG 1 cut(s) 214
NdeII GATC 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 319
NsiI ATGCAT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 3 cut(s) 28, 251, 300
Ppu21I YACGTR 1 cut(s) 417
PspPI GGNCC 1 cut(s) 461
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SatI GCNGC 3 cut(s) 27, 250, 299
Sau3AI GATC 1 cut(s) 132
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 1 cut(s) 416
SfaNI GCATC 2 cut(s) 199, 250
SinI GGWCC 1 cut(s) 461
Sse9I AATT 3 cut(s) 107, 168, 189
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 319, 422
TaiI ACGT 2 cut(s) 419, 479
TaqI TCGA 2 cut(s) 207, 239
TaqII GACCGA 1 cut(s) 392
TasI AATT 3 cut(s) 107, 168, 189
TfiI GAWTC 1 cut(s) 112
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 26, 249, 298
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 2 cut(s) 99, 265
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.