pycom03g23110

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008322
Physical Location & Seq
Reverse (-)
23060460 .. 23061355
896 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23110.1

Sequence Viewer

Length: 606 bp
ATGGCTCTCACCTCCCGTTTCCTCTTTGCCACCTCCTCCTCCATGCTTCTCTTCTCATTGCTAGCCATTGCCTCTGCTACGGATTATAGCTCCGGTGCTCCTAAGCCAAAGGTTGATGAGCATCCAAAACTTCCGAAGGTTGGTATGCCGAAATTACAGAATGTCGGAAAACCGAAACCAGAAGTATATACAAAGCTAAAACCTCAGACCATTGATATTCAGGGGCTGGTTTTGTGCAGATCAGGCCTTCAAAGCTTCCCAATTAAAGGAGCTGTGGTAAGGATTACATGTTTGGCTGAGGATGAACAAGGTTACGAGACTGCTCCTTTCTCCATATTGAGTGGTGCAACTGATGCAAAGGGTTACTTCATCTCAACGTTGTCGCCGTCGCAGCTTGGAGACAAGTGGAAGCTCACAGAGTGCAAGGCGTTTCTCGATTATTCTCCATTGGAGACCTGCAAAGTCCCTACTGATGTTAACCAAGGAATTACTGGTCATCTGCTTTCTTCTTATAGTACCCTCAGTGCCAAGAACATCAAGCTGTACTCAGTTGGGCCTTTCTTCTGCACCTCAGAATCTAAACCAGTTCTTAATGGCGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

202

Amino Acids

21.68

Weight (kDa)

8.88

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 73 - 165 1.6e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 464
AciI CCGC 1 cut(s) 597
AclI AACGTT 1 cut(s) 377
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 2 cut(s) 517, 545
AfiI CCNNNNNNNGG 2 cut(s) 140, 266
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 1 cut(s) 251
AluBI AGCT 7 cut(s) 90, 196, 255, 272, 394, 412, 541
AluI AGCT 7 cut(s) 90, 196, 255, 272, 394, 412, 541
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 3 cut(s) 311, 393, 446
AlwNI CAGNNNCTG 1 cut(s) 226
AoxI GGCC 2 cut(s) 244, 554
ApeKI GCWGC 1 cut(s) 391
AspS9I GGNCC 1 cut(s) 554
AsuNHI GCTAGC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 100
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 403
BceAI ACGGC 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 311, 393, 446
BfaI CTAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 464
BisI GCNGC 2 cut(s) 392, 598
BlsI GCNGC 2 cut(s) 393, 599
BmgT120I GGNCC 1 cut(s) 554
BmsI GCATC 2 cut(s) 130, 343
BmtI GCTAGC 1 cut(s) 65
Bpu10I CCTNAGC 2 cut(s) 102, 297
BsaBI GATNNNNATC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 266
Bse1I ACTGG 2 cut(s) 496, 584
Bse3DI GCAATG 2 cut(s) 56, 66
Bse8I GATNNNNATC 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 481
BseGI GGATG 2 cut(s) 121, 307
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 140, 266
BseMI GCAATG 2 cut(s) 56, 66
BseMII CTCAG 5 cut(s) 218, 288, 535, 561, 585
BseNI ACTGG 2 cut(s) 496, 584
BseRI GAGGAG 2 cut(s) 25, 28
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 2 cut(s) 256, 550
BshFI GGCC 2 cut(s) 246, 556
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 2 cut(s) 140, 266
BsmAI GTCTC 3 cut(s) 311, 393, 446
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 2 cut(s) 246, 556
Bso31I GGTCTC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 100
Bsp143I GATC 1 cut(s) 239
BspACI CCGC 1 cut(s) 597
BspANI GGCC 2 cut(s) 246, 556
BspCNI CTCAG 5 cut(s) 217, 289, 534, 560, 584
BspMI ACCTGC 1 cut(s) 464
BspOI GCTAGC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 2 cut(s) 56, 66
BsrI ACTGG 2 cut(s) 496, 584
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 6 cut(s) 102, 204, 297, 521, 547, 571
BstF5I GGATG 2 cut(s) 121, 307
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 3 cut(s) 311, 393, 446
BstMBI GATC 1 cut(s) 239
BstMWI GCNNNNNNNGC 4 cut(s) 243, 252, 353, 391
BstNSI RCATGY 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 403
BsuRI GGCC 2 cut(s) 246, 556
BtsCI GGATG 2 cut(s) 121, 307
BtsIMutI CAGTG 1 cut(s) 529
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 63
CaiI CAGNNNCTG 1 cut(s) 226
Cfr13I GGNCC 1 cut(s) 554
Csp6I GTAC 2 cut(s) 516, 544
CviAII CATG 2 cut(s) 43, 288
CviQI GTAC 2 cut(s) 516, 544
DdeI CTNAG 6 cut(s) 102, 204, 297, 521, 547, 571
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
DraIII CACNNNGTG 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 2 cut(s) 46, 291
FaiI YATR 8 cut(s) 44, 87, 146, 187, 189, 289, 335, 513
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 449
FatI CATG 2 cut(s) 42, 287
Fnu4HI GCNGC 2 cut(s) 392, 598
FokI GGATG 2 cut(s) 108, 314
Fsp4HI GCNGC 2 cut(s) 392, 598
FspBI CTAG 1 cut(s) 62
GluI GCNGC 2 cut(s) 392, 598
HaeIII GGCC 2 cut(s) 246, 556
HapII CCGG 1 cut(s) 93
Hin1II CATG 2 cut(s) 46, 291
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 1 cut(s) 575
HpaI GTTAAC 1 cut(s) 478
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 4 cut(s) 135, 167, 207, 574
Hpy188III TCNNGA 1 cut(s) 434
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 1 cut(s) 391
HpyAV CCTTC 2 cut(s) 130, 257
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 6 cut(s) 237, 347, 356, 423, 459, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 252, 353, 391
HpyF3I CTNAG 6 cut(s) 102, 204, 297, 521, 547, 571
HpySE526I ACGT 1 cut(s) 377
Hsp92II CATG 2 cut(s) 46, 291
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 4 cut(s) 95, 103, 269, 328
LpnPI CCDG 8 cut(s) 106, 192, 206, 212, 228, 469, 477, 597
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 2 cut(s) 130, 343
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 2 cut(s) 311, 362
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 3 cut(s) 43, 498, 553
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 3 cut(s) 152, 261, 486
MmeI TCCRAC 1 cut(s) 145
MseI TTAA 4 cut(s) 264, 477, 591, 604
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 243, 252, 353, 391
NdeII GATC 1 cut(s) 239
NheI GCTAGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 46, 291
NspI RCATGY 1 cut(s) 291
PceI AGGCCT 1 cut(s) 246
PciI ACATGT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 2 cut(s) 393, 599
PscI ACATGT 1 cut(s) 287
Psp1406I AACGTT 1 cut(s) 377
PspPI GGNCC 1 cut(s) 554
PstNI CAGNNNCTG 1 cut(s) 226
RsaI GTAC 2 cut(s) 517, 545
RsaNI GTAC 2 cut(s) 516, 544
SaqAI TTAA 4 cut(s) 264, 477, 591, 604
SatI GCNGC 2 cut(s) 392, 598
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 554
SduI GDGCHC 1 cut(s) 100
SfaNI GCATC 2 cut(s) 130, 343
Sse9I AATT 3 cut(s) 152, 261, 486
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 1 cut(s) 597
SspMI CTAG 1 cut(s) 62
StuI AGGCCT 1 cut(s) 246
StyI CCWWGG 1 cut(s) 481
TaiI ACGT 1 cut(s) 380
TaqI TCGA 1 cut(s) 435
TasI AATT 3 cut(s) 152, 261, 486
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 4 cut(s) 264, 477, 591, 604
Tru9I TTAA 4 cut(s) 264, 477, 591, 604
TscAI CASTG 1 cut(s) 529
TseI GCWGC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 318, 358
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 1 cut(s) 529
XceI RCATGY 1 cut(s) 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.