MD11G1306600.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008322
Physical Location & Seq
Forward (+)
42084701 .. 42085920
1220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1306600.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGCTGTCAAGTCCCGTTTCCTCTTTGCCACCTCCTCCTCCATGCTTCTCTTCTCATTGCTAGTCATTGCCTCTGCGAAGGATTATAGCTCCGGTGCCCCTAGACCTAAGGTTGATGAGTATCCAGAACTTCCAAAGGTTGATGGGTATCCAGAACTTCCAAAGGTTGATATTCCGAACTTACCGAACGCCGGAAAACCGAAACCAGAAGTATATGCAAAGCTAAAACCTCAGATCATTGGTATTCAGGGGCTGGTTTTGTGCAAATCAGGCCTTCAAAGCTTCCCAATTAAAGGAGCTGTGGTAAGGATTACATGTCTGGCTGAGGATGAACAAGGTTACGAGACCGCTCCTTTCTCCAAACTGAGTGGTGCAACTGATGCAAAGGGATACTTCTTCGCAACGTTATCGCCGTCGCAGCTTGAAGACAAGTGGAAGCTCACAGAGTGCAAGGCGTTCCTCGATTATTCTCCATTGGAAGCCTGCAAAGTCCCTACTGACGTTAACCATGGAATTACTGGTCATCTTCTTTCTTCTTATCGTGCCCTCAGTGCCAAGAACATCAAGTTGTACTCGGTTGGGCCTTTCTTTTGCACCTCGGAAACTAAATCAGTCCCTAACGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

210

Amino Acids

22.72

Weight (kDa)

8.69

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 82 - 174 6.3e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 350
AciI CCGC 1 cut(s) 348
AclI AACGTT 1 cut(s) 404
AdeI CACNNNGTG 1 cut(s) 447
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 2 cut(s) 191, 293
AflIII ACRYGT 1 cut(s) 314
AgsI TTSAA 2 cut(s) 278, 425
AluBI AGCT 6 cut(s) 90, 223, 282, 299, 421, 439
AluI AGCT 6 cut(s) 90, 223, 282, 299, 421, 439
Alw26I GTCTC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 253
AoxI GGCC 2 cut(s) 271, 581
ApeKI GCWGC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 2 cut(s) 100, 547
BanI GGYRCC 1 cut(s) 95
BbsI GAAGAC 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 430
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 397
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciVI GTATCC 3 cut(s) 132, 159, 383
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 2 cut(s) 62, 102
BfuI GTATCC 3 cut(s) 132, 159, 383
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 97
BmsI GCATC 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 324
BsaBI GATNNNNATC 2 cut(s) 120, 147
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 508, 597
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 293
Bse1I ACTGG 1 cut(s) 523
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 2 cut(s) 56, 66
Bse8I GATNNNNATC 2 cut(s) 120, 147
BseDI CCNNGG 2 cut(s) 508, 597
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 2 cut(s) 120, 147
BseLI CCNNNNNNNGG 2 cut(s) 191, 293
BseMI GCAATG 2 cut(s) 56, 66
BseMII CTCAG 4 cut(s) 245, 315, 356, 562
BseNI ACTGG 1 cut(s) 523
BseRI GAGGAG 2 cut(s) 25, 28
BseSI GKGCMC 2 cut(s) 100, 547
BseXI GCAGC 1 cut(s) 430
BshFI GGCC 2 cut(s) 273, 583
BshNI GGYRCC 1 cut(s) 95
BsiSI CCGG 2 cut(s) 93, 192
BslFI GGGAC 2 cut(s) 476, 599
BslI CCNNNNNNNGG 2 cut(s) 191, 293
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 2 cut(s) 476, 599
BsnI GGCC 2 cut(s) 273, 583
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 100, 547
Bsp143I GATC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 1 cut(s) 348
BspANI GGCC 2 cut(s) 273, 583
BspCNI CTCAG 4 cut(s) 244, 316, 357, 561
BspLI GGNNCC 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 2 cut(s) 56, 66
BsrI ACTGG 1 cut(s) 523
BssECI CCNNGG 2 cut(s) 508, 597
BssMI GATC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 5 cut(s) 108, 231, 324, 365, 548
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 1 cut(s) 334
BstKTI GATC 1 cut(s) 237
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 1 cut(s) 234
BstMWI GCNNNNNNNGC 5 cut(s) 270, 279, 380, 418, 551
BstNSI RCATGY 1 cut(s) 318
BstSLI GKGCMC 2 cut(s) 100, 547
BstV1I GCAGC 1 cut(s) 430
BstV2I GAAGAC 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 108
BsuI GTATCC 3 cut(s) 132, 159, 383
BsuRI GGCC 2 cut(s) 273, 583
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 1 cut(s) 484
CaiI CAGNNNCTG 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 571
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 3 cut(s) 43, 315, 509
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 5 cut(s) 108, 231, 324, 365, 548
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
DraIII CACNNNGTG 1 cut(s) 447
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 508
Eco147I AGGCCT 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 338
Eco81I CCTNAGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 3 cut(s) 46, 318, 512
FaiI YATR 6 cut(s) 44, 87, 214, 216, 316, 510
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 2 cut(s) 476, 599
FatI CATG 3 cut(s) 42, 314, 508
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 2 cut(s) 62, 102
GluI GCNGC 1 cut(s) 419
HaeIII GGCC 2 cut(s) 273, 583
HapII CCGG 2 cut(s) 93, 192
Hin1II CATG 3 cut(s) 46, 318, 512
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 280
HpaI GTTAAC 1 cut(s) 505
HpaII CCGG 2 cut(s) 93, 192
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 3 cut(s) 177, 234, 601
Hpy188III TCNNGA 2 cut(s) 125, 152
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 2 cut(s) 73, 284
HpyCH4IV ACGT 2 cut(s) 404, 501
HpyCH4V TGCA 7 cut(s) 218, 264, 374, 383, 450, 486, 594
HpyF10VI GCNNNNNNNGC 5 cut(s) 270, 279, 380, 418, 551
HpyF3I CTNAG 5 cut(s) 108, 231, 324, 365, 548
HpySE526I ACGT 2 cut(s) 404, 501
Hsp92II CATG 3 cut(s) 46, 318, 512
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 1 cut(s) 234
LmnI GCTCC 3 cut(s) 95, 296, 355
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 1 cut(s) 370
MaeI CTAG 2 cut(s) 62, 102
MaeII ACGT 2 cut(s) 404, 501
MaeIII GTNAC 1 cut(s) 338
MalI GATC 1 cut(s) 236
MbiI CCGCTC 1 cut(s) 350
MboI GATC 1 cut(s) 234
MboII GAAGA 5 cut(s) 43, 388, 437, 518, 525
MhlI GDGCHC 2 cut(s) 100, 547
MluCI AATT 2 cut(s) 288, 513
MseI TTAA 3 cut(s) 291, 504, 628
MspI CCGG 2 cut(s) 93, 192
MwoI GCNNNNNNNGC 5 cut(s) 270, 279, 380, 418, 551
NcoI CCATGG 1 cut(s) 508
NdeII GATC 1 cut(s) 234
NlaIII CATG 3 cut(s) 46, 318, 512
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 318
PceI AGGCCT 1 cut(s) 273
PciI ACATGT 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PkrI GCNGC 1 cut(s) 420
PscI ACATGT 1 cut(s) 314
Psp1406I AACGTT 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 581
PstNI CAGNNNCTG 1 cut(s) 253
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SaqAI TTAA 3 cut(s) 291, 504, 628
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 1 cut(s) 234
Sau96I GGNCC 1 cut(s) 581
SduI GDGCHC 2 cut(s) 100, 547
SfaNI GCATC 1 cut(s) 370
Sse9I AATT 2 cut(s) 288, 513
SseBI AGGCCT 1 cut(s) 273
SsiI CCGC 1 cut(s) 348
SspMI CTAG 2 cut(s) 62, 102
StuI AGGCCT 1 cut(s) 273
StyI CCWWGG 1 cut(s) 508
TaiI ACGT 2 cut(s) 407, 504
TaqI TCGA 1 cut(s) 462
TasI AATT 2 cut(s) 288, 513
TatI WGTACW 1 cut(s) 570
Tru1I TTAA 3 cut(s) 291, 504, 628
Tru9I TTAA 3 cut(s) 291, 504, 628
TscAI CASTG 1 cut(s) 556
TseI GCWGC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 1 cut(s) 556
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XspI CTAG 2 cut(s) 62, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.