Rmu_ssc0000327.1_g000004

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000327.1
OG0008322
Physical Location & Seq
Reverse (-)
10425 .. 11299
875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000327.1_g000004.1.cds

Sequence Viewer

Length: 636 bp
atggctctcactcggttcctcctccccacctgtctggttttactttcattgctactcatcaacgtctcagctactgattatggctatggcccaaaaccaccacaggttgagcaaccaaaacctccacaggtccagaaaccagaaccaccacaggccgagaaaccaaaaccaccacaggttgagaaaccaaaactagatcagttgtacggagagctactacctggcaatctgttcggcattcaagggcttgttttatgtaactcaggacttaaagccttcccaattcaaggagctgtggcaaggattacatgcgtgggcgaggacgaaaatgggtacgagactgcacctttctccatcttaagtggtgcaactgatgcaaagggttacttctttgcaacattgtcaccttcaaagcttgaaggtaattacaacaataagtggaagctcaccgagtgcaagacattcctcgacaattctccactggagagctgcaaagtccctactgatgtcaaccatggaattagtggggctccgattgcttcttttcgcactctcaatgccaagaacatgaagcttttctctgttggacctttcttctactcctcagaacctaaaccagcccctagtggctattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

211

Amino Acids

22.86

Weight (kDa)

8.2

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AdeI CACNNNGTG 1 cut(s) 453
AfaI GTAC 2 cut(s) 206, 335
AfiI CCNNNNNNNGG 1 cut(s) 287
AflII CTTAAG 1 cut(s) 358
AgsI TTSAA 4 cut(s) 242, 287, 411, 419
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 7 cut(s) 71, 214, 293, 415, 445, 489, 574
AluI AGCT 7 cut(s) 71, 214, 293, 415, 445, 489, 574
Alw26I GTCTC 2 cut(s) 70, 332
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 2 cut(s) 88, 153
ApeKI GCWGC 1 cut(s) 489
Asp700I GAANNNNTTC 1 cut(s) 575
AspS9I GGNCC 3 cut(s) 89, 130, 587
AsuHPI GGTGA 2 cut(s) 396, 439
AvaII GGWCC 2 cut(s) 130, 587
BanII GRGCYC 1 cut(s) 532
BbvI GCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 362
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 70, 332
BfaI CTAG 2 cut(s) 194, 624
BfrI CTTAAG 1 cut(s) 358
BisI GCNGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 491
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 2 cut(s) 130, 587
BmgT120I GGNCC 3 cut(s) 89, 130, 587
BmiI GGNNCC 2 cut(s) 17, 531
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 503
BsaJI CCNNGG 1 cut(s) 514
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 47
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 3 cut(s) 81, 276, 618
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 2 cut(s) 11, 592
BseXI GCAGC 1 cut(s) 476
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 2 cut(s) 90, 155
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 2 cut(s) 70, 332
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 2 cut(s) 90, 155
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 1 cut(s) 196
Bsp19I CCATGG 1 cut(s) 514
BspANI GGCC 2 cut(s) 90, 155
BspCNI CTCAG 3 cut(s) 80, 275, 617
BspLI GGNNCC 2 cut(s) 17, 531
BspTI CTTAAG 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 1 cut(s) 196
BssT1I CCWWGG 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 222
BstAFI CTTAAG 1 cut(s) 358
BstAPI GCANNNNNTGC 1 cut(s) 374
BstDEI CTNAG 3 cut(s) 67, 262, 604
BstDSI CCRYGG 1 cut(s) 514
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 2 cut(s) 70, 332
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 374, 536
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 476
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 2 cut(s) 90, 155
BtgI CCRYGG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 479
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 89, 130, 587
Csp6I GTAC 2 cut(s) 205, 334
CviAII CATG 3 cut(s) 309, 515, 568
CviQI GTAC 2 cut(s) 205, 334
DdeI CTNAG 3 cut(s) 67, 262, 604
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
DraIII CACNNNGTG 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 514
Eco24I GRGCYC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 130, 587
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 3 cut(s) 312, 518, 571
FaiI YATR 6 cut(s) 81, 87, 256, 310, 516, 569
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 482
FatI CATG 3 cut(s) 308, 514, 567
Fnu4HI GCNGC 1 cut(s) 490
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 1 cut(s) 490
FspBI CTAG 2 cut(s) 194, 624
GluI GCNGC 1 cut(s) 490
GsuI CTGGAG 1 cut(s) 503
HaeIII GGCC 2 cut(s) 90, 155
Hin1II CATG 3 cut(s) 312, 518, 571
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 2 cut(s) 413, 572
HphI GGTGA 2 cut(s) 396, 439
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 2 cut(s) 534, 607
Hpy188III TCNNGA 2 cut(s) 133, 264
Hpy8I GTNNAC 1 cut(s) 511
HpyAV CCTTC 3 cut(s) 286, 413, 417
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 6 cut(s) 344, 368, 377, 395, 456, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 536
HpyF3I CTNAG 3 cut(s) 67, 262, 604
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 3 cut(s) 312, 518, 571
Kzo9I GATC 1 cut(s) 196
LmnI GCTCC 2 cut(s) 290, 535
Lsp1109I GCAGC 1 cut(s) 476
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 194, 624
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 3 cut(s) 257, 383, 402
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 4 cut(s) 282, 424, 472, 519
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 6 cut(s) 29, 32, 132, 313, 476, 613
MroXI GAANNNNTTC 1 cut(s) 575
MseI TTAA 2 cut(s) 270, 359
MspCI CTTAAG 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 374, 536
NcoI CCATGG 1 cut(s) 514
NdeII GATC 1 cut(s) 196
NlaIII CATG 3 cut(s) 312, 518, 571
NlaIV GGNNCC 2 cut(s) 17, 531
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 402
NspI RCATGY 1 cut(s) 312
PctI GAATGC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 575
PkrI GCNGC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 17, 531
PspPI GGNCC 3 cut(s) 89, 130, 587
PstNI CAGNNNCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 206, 335
RsaNI GTAC 2 cut(s) 205, 334
SaqAI TTAA 2 cut(s) 270, 359
SatI GCNGC 1 cut(s) 490
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 3 cut(s) 89, 130, 587
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 2 cut(s) 130, 587
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 4 cut(s) 282, 424, 472, 519
SspMI CTAG 2 cut(s) 194, 624
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 514
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 468
TasI AATT 4 cut(s) 282, 424, 472, 519
Tru1I TTAA 2 cut(s) 270, 359
Tru9I TTAA 2 cut(s) 270, 359
TscAI CASTG 1 cut(s) 486
TseFI GTSAC 1 cut(s) 402
TseI GCWGC 1 cut(s) 489
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 2 cut(s) 36, 584
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 486
Vha464I CTTAAG 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 130, 587
XceI RCATGY 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmnI GAANNNNTTC 1 cut(s) 575
XspI CTAG 2 cut(s) 194, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.