Rroxscaffold_7G00173360

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008322
Physical Location & Seq
Forward (+)
13398612 .. 13399627
1016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173360.1

Sequence Viewer

Length: 750 bp
ATGGCTCTCACTCGCTTCCTCCTCCCCAGCTGTCTGGTTTTACTTTCATTGCTACTCATCAACGTCTCTGCTACTGATTATGGCTATGGCGCAAAACCACCACAGGTTGAGCAACCAAAACCTCCACAGGTCCAGAAACCAGAACCACCACAGGTCGAGAAACCAAAACCACCACAGGTTGAGCAACCAAAATCTCGGCAGGTCCAGAAACCAAAACCACCACAGGTTGAGAAACCAACACCTCCACAGGTCTCTACTAATGATTATGGCTATGGCCCAAACCCACCACAGGTTGAGAAACCAAAACAAGATCAGTTGTACGGAGAGCTACTACCTCACAATCTGTTCGGCATTCAGGGGCTTGTTTTATGTAACTCAGGACTTAAAGCCTTCCCAATTCAAGGAGCTGTGGCAAGGATTACATGCATGGGCGAGGACGAAAATGGGTACGAGACTGCACCTTTCTCCATCTTAAGTGGTGCAACTGATGCGAAGGGTTACTTCTTTGCAACATTGTCACCTTCAAAGCTTGAAGGTAATTACAACAATAAGTGGAAGCTCACAGAGTGCAAGACATTCCTCGACAATTCTCCACTGGAGAGCTGCAAAGTCCCTACTGATGTCAACCATGGAATTAGTGGTGCTCCGATTGCTTCTTATCGCACTCTCAATGCCAAGAACATGAAGCTGTTCTCTGTTGGACCCTTCTTCTACTCCTCAGAACCTAAACCAGCCCCTAGTGGCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

249

Amino Acids

27.22

Weight (kDa)

8.67

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 118 - 214 9.8e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 190
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 2 cut(s) 320, 449
AfiI CCNNNNNNNGG 2 cut(s) 289, 401
AflII CTTAAG 1 cut(s) 472
AgsI TTSAA 3 cut(s) 401, 525, 533
AluBI AGCT 7 cut(s) 30, 328, 407, 529, 559, 603, 688
AluI AGCT 7 cut(s) 30, 328, 407, 529, 559, 603, 688
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 70, 256, 446
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 603
Asp700I GAANNNNTTC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 4 cut(s) 130, 202, 275, 701
AsuHPI GGTGA 1 cut(s) 510
AvaII GGWCC 3 cut(s) 130, 202, 701
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 1 cut(s) 590
BccI CCATC 1 cut(s) 476
BcoDI GTCTC 3 cut(s) 70, 256, 446
BfaI CTAG 1 cut(s) 738
BfrI CTTAAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 1 cut(s) 604
BlsI GCNGC 1 cut(s) 605
Bme18I GGWCC 3 cut(s) 130, 202, 701
BmgT120I GGNCC 4 cut(s) 130, 202, 275, 701
BmiI GGNNCC 1 cut(s) 703
BmsI GCATC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 617
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 1 cut(s) 628
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 401
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 628
BseLI CCNNNNNNNGG 2 cut(s) 289, 401
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 390, 732
BseNI ACTGG 1 cut(s) 600
BseRI GAGGAG 2 cut(s) 11, 706
BseXI GCAGC 1 cut(s) 590
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 646
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 2 cut(s) 289, 401
BsmAI GTCTC 3 cut(s) 70, 256, 446
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 628
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 2 cut(s) 389, 731
BspLI GGNNCC 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 190
BspTI CTTAAG 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 1 cut(s) 600
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 628
BstAFI CTTAAG 1 cut(s) 472
BstAPI GCANNNNNTGC 1 cut(s) 488
BstDEI CTNAG 2 cut(s) 376, 718
BstDSI CCRYGG 1 cut(s) 628
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 3 cut(s) 70, 256, 446
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 2 cut(s) 488, 650
BstNSI RCATGY 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 590
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 593
BveI ACCTGC 1 cut(s) 190
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 130, 202, 275, 701
Csp6I GTAC 2 cut(s) 319, 448
CviAII CATG 4 cut(s) 423, 427, 629, 682
CviQI GTAC 2 cut(s) 319, 448
DdeI CTNAG 2 cut(s) 376, 718
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
DraIII CACNNNGTG 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 130, 202, 701
EcoT14I CCWWGG 1 cut(s) 628
EcoT22I ATGCAT 1 cut(s) 428
ErhI CCWWGG 1 cut(s) 628
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 426, 430, 632, 685
FaiI YATR 9 cut(s) 81, 87, 267, 273, 370, 424, 428, 630, 683
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 1 cut(s) 596
FatI CATG 4 cut(s) 422, 426, 628, 681
Fnu4HI GCNGC 1 cut(s) 604
Fsp4HI GCNGC 1 cut(s) 604
FspBI CTAG 1 cut(s) 738
GlaI GCGC 1 cut(s) 91
GluI GCNGC 1 cut(s) 604
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 1 cut(s) 276
HhaI GCGC 1 cut(s) 92
Hin1II CATG 4 cut(s) 426, 430, 632, 685
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 527
HphI GGTGA 1 cut(s) 510
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 2 cut(s) 648, 721
Hpy188III TCNNGA 4 cut(s) 133, 157, 205, 378
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 5 cut(s) 400, 487, 527, 531, 715
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 6 cut(s) 426, 458, 482, 509, 570, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 650
HpyF3I CTNAG 2 cut(s) 376, 718
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 4 cut(s) 426, 430, 632, 685
HspAI GCGC 1 cut(s) 90
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 404, 649
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 1 cut(s) 478
MaeI CTAG 1 cut(s) 738
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 3 cut(s) 371, 497, 516
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 1 cut(s) 700
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 4 cut(s) 396, 538, 586, 633
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 8 cut(s) 29, 32, 132, 252, 345, 427, 590, 727
Mph1103I ATGCAT 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 2 cut(s) 384, 473
MspA1I CMGCKG 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 488, 650
NcoI CCATGG 1 cut(s) 628
NdeII GATC 1 cut(s) 310
NlaIII CATG 4 cut(s) 426, 430, 632, 685
NlaIV GGNNCC 1 cut(s) 703
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 428
NspI RCATGY 1 cut(s) 426
PctI GAATGC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 689
PkrI GCNGC 1 cut(s) 605
PspFI CCCAGC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 4 cut(s) 130, 202, 275, 701
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 2 cut(s) 320, 449
RsaNI GTAC 2 cut(s) 319, 448
SaqAI TTAA 2 cut(s) 384, 473
SatI GCNGC 1 cut(s) 604
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 4 cut(s) 130, 202, 275, 701
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 1 cut(s) 478
SinI GGWCC 3 cut(s) 130, 202, 701
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
Sse9I AATT 4 cut(s) 396, 538, 586, 633
SspMI CTAG 1 cut(s) 738
StyI CCWWGG 1 cut(s) 628
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 156, 582
TasI AATT 4 cut(s) 396, 538, 586, 633
Tru1I TTAA 2 cut(s) 384, 473
Tru9I TTAA 2 cut(s) 384, 473
TscAI CASTG 1 cut(s) 600
TseFI GTSAC 1 cut(s) 516
TseI GCWGC 1 cut(s) 603
Tsp45I GTSAC 1 cut(s) 516
TspDTI ATGAA 2 cut(s) 36, 698
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 1 cut(s) 600
Vha464I CTTAAG 1 cut(s) 472
VpaK11BI GGWCC 3 cut(s) 130, 202, 701
XceI RCATGY 1 cut(s) 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 1 cut(s) 738
Zsp2I ATGCAT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.