FvH4_2g26070

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008322
Physical Location & Seq
Reverse (-)
20983866 .. 20984877
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26070.t1

Sequence Viewer

Length: 633 bp
ATGGCTCTCACTCGCTTCCTCTTTCCCACCTGTCTGGTTGTACTTTCATTGCTACTTATCGACGTCTCTGCTGATTATGGCTATGCCTCAAAACCACCACAGGTTGAGCAACCAAAACCTCCACAGGTTCAAAAGCCAGAAGCACCACAGGTTGAGAAACCAAATCCACCACAGGTTGAGAAACCAAAACTAGATCAATCGTATGGAGAACTACTACCTCACAATCTCTTCGGCATTCAAGGGCTTGTTTTATGTACCTCAGGCCTTAAAGCTTTCCCAATTCAAGGAGCTGTGGCAAGGATTACATGCGTGGGAGAGGACGAAAATGGGTATGAGACTGCACCTTTCTCCATGTTAGCTGGTGCAACTGATGCCAAGGGTTACTTCTTTGCAACATTGTCACCGTCAAAGCTTGAAGATAGTTACAACAAGAAATGGAAGCTCACCGAGTGCAAGGCATTCCTTGACAGTTCTCCATTGGAGAGCTGCAAAGTCCCTACTGACGCCAACCATGGAATCAGTGGTGCTCCTCTTGCTTCTTATCGCACGCTCAATGCCAAGAACATGAAGTTGTTCTCTGTTGGACCCTTCTTCTACACCTCAGAACCTAAACCAGCCCCTAATGGCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

211

Amino Acids

22.83

Weight (kDa)

6.72

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 79 - 175 2.7e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AcyI GRCGYC 2 cut(s) 63, 504
AdeI CACNNNGTG 1 cut(s) 450
AfaI GTAC 2 cut(s) 42, 256
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 4 cut(s) 131, 239, 284, 416
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 6 cut(s) 272, 290, 359, 412, 442, 486
AluI AGCT 6 cut(s) 272, 290, 359, 412, 442, 486
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 2 cut(s) 70, 329
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 2 cut(s) 393, 436
AvaII GGWCC 1 cut(s) 584
AxyI CCTNAGG 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 473
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BcoDI GTCTC 2 cut(s) 70, 329
BfaI CTAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 487
BlsI GCNGC 1 cut(s) 488
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 1 cut(s) 586
BmsI GCATC 1 cut(s) 361
BsaHI GRCGYC 2 cut(s) 63, 504
BsaJI CCNNGG 2 cut(s) 375, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse21I CCTNAGG 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 375, 511
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 273, 615
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 529
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 2 cut(s) 70, 329
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 2 cut(s) 234, 458
BsnI GGCC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 1 cut(s) 193
Bsp19I CCATGG 1 cut(s) 511
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 272, 614
BspLI GGNNCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 375, 511
BssMI GATC 1 cut(s) 193
BssNI GRCGYC 2 cut(s) 63, 504
BssT1I CCWWGG 2 cut(s) 375, 511
Bst4CI ACNGT 2 cut(s) 405, 470
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 2 cut(s) 63, 504
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 2 cut(s) 259, 601
BstDSI CCRYGG 1 cut(s) 511
BstKTI GATC 1 cut(s) 196
BstMAI GTCTC 2 cut(s) 70, 329
BstMBI GATC 1 cut(s) 193
BstMWI GCNNNNNNNGC 2 cut(s) 371, 533
BstNSI RCATGY 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 34
Bsu36I CCTNAGG 1 cut(s) 259
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 511
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 548
Cfr13I GGNCC 1 cut(s) 584
CseI GACGC 1 cut(s) 512
Csp6I GTAC 2 cut(s) 41, 255
CviAII CATG 4 cut(s) 306, 352, 512, 565
CviQI GTAC 2 cut(s) 41, 255
DdeI CTNAG 2 cut(s) 259, 601
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
DraIII CACNNNGTG 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 2 cut(s) 375, 511
Eco147I AGGCCT 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 584
Eco81I CCTNAGG 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 375, 511
ErhI CCWWGG 2 cut(s) 375, 511
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 309, 355, 515, 568
FaiI YATR 9 cut(s) 78, 84, 204, 253, 307, 333, 353, 513, 566
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 479
FatI CATG 4 cut(s) 305, 351, 511, 564
Fnu4HI GCNGC 1 cut(s) 487
Fsp4HI GCNGC 1 cut(s) 487
FspBI CTAG 1 cut(s) 191
GluI GCNGC 1 cut(s) 487
HaeIII GGCC 1 cut(s) 264
HgaI GACGC 1 cut(s) 512
Hin1I GRCGYC 2 cut(s) 63, 504
Hin1II CATG 4 cut(s) 309, 355, 515, 568
HindIII AAGCTT 2 cut(s) 270, 410
HinfI GANTC 1 cut(s) 516
HphI GGTGA 2 cut(s) 393, 436
Hpy188I TCNGA 1 cut(s) 604
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 1 cut(s) 598
HpyCH4III ACNGT 2 cut(s) 405, 470
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 5 cut(s) 341, 365, 392, 453, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 533
HpyF3I CTNAG 2 cut(s) 259, 601
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 2 cut(s) 63, 504
Hsp92II CATG 4 cut(s) 309, 355, 515, 568
Kzo9I GATC 1 cut(s) 193
LmnI GCTCC 2 cut(s) 287, 532
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 1 cut(s) 361
MaeI CTAG 1 cut(s) 191
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 3 cut(s) 380, 399, 422
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 3 cut(s) 220, 428, 583
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 1 cut(s) 279
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 8 cut(s) 29, 97, 129, 228, 268, 310, 540, 610
MroXI GAANNNNTTC 1 cut(s) 572
MseI TTAA 1 cut(s) 267
Mva1269I GAATGC 2 cut(s) 234, 458
MwoI GCNNNNNNNGC 2 cut(s) 371, 533
NcoI CCATGG 1 cut(s) 511
NdeII GATC 1 cut(s) 193
NlaIII CATG 4 cut(s) 309, 355, 515, 568
NlaIV GGNNCC 1 cut(s) 586
NmuCI GTSAC 1 cut(s) 399
NspI RCATGY 1 cut(s) 309
PceI AGGCCT 1 cut(s) 264
PctI GAATGC 2 cut(s) 234, 458
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 516
PkrI GCNGC 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 1 cut(s) 584
RsaI GTAC 2 cut(s) 42, 256
RsaNI GTAC 2 cut(s) 41, 255
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 487
Sau3AI GATC 1 cut(s) 193
Sau96I GGNCC 1 cut(s) 584
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 1 cut(s) 361
SinI GGWCC 1 cut(s) 584
Sse9I AATT 1 cut(s) 279
SseBI AGGCCT 1 cut(s) 264
SspMI CTAG 1 cut(s) 191
StuI AGGCCT 1 cut(s) 264
StyI CCWWGG 2 cut(s) 375, 511
TaaI ACNGT 2 cut(s) 405, 470
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 60
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 1 cut(s) 516
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 1 cut(s) 486
Tsp45I GTSAC 1 cut(s) 399
TspDTI ATGAA 2 cut(s) 36, 581
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 584
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 1 cut(s) 191
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.