MD03G1291700.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008322
Physical Location & Seq
Reverse (-)
36910776 .. 36912036
1261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1291700.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGCTCTCACCTCCCGTTTCCTCTTTGCCACCTCCTCCTCCATGCTTCTCTTCTCATTGCTAGCCATTGCCTCTGCTACGGATTATAGCTCCGGTGCGCCTAAGCCAAAGGTTGATGAGCATCCAAAACTTCCAAAGGTTGATATGCCAAAATTACCGAATGTCGGAAAACCAAAACCAGAAGTATATACAAAGCTAAAACCTCAGACCATTGATATTCAGGGGCTGGTTTTGTGCAGATCAGGCCTTCAAAGCTTCCCAATTAAAGCTGTGGTAAGGATTACATGTTTGGCTGAGGATGAACAAGGTTACGAGACTGCTCCTTTCTCCATATTGAGTGGTGCAACTGATGCAAAGGGTTACTTCATCTCAACGTTGTCGCCGTCGCAGCTTGGAGACAAGTGGAAGCTCACAGAGTGCAAGGCGTTTCTCGATTATTCTCCATTGGAGACCTGCAAAGTCCCTACTGACGTTAACCAAGGAATTACTGGTCATCTGCTTTCTTCTTATCGTACCCTCAGTGCCAAGAACATCAAGCTGTACTCTGTTGGGCCTTTCTTCTGCAGCTCAAAACCTAAACCAGTTCCTAATGGTGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

201

Amino Acids

21.7

Weight (kDa)

9.12

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 73 - 164 9.9e-16 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 461
AclI AACGTT 1 cut(s) 374
AdeI CACNNNGTG 1 cut(s) 417
AfaI GTAC 2 cut(s) 514, 542
AfiI CCNNNNNNNGG 2 cut(s) 164, 593
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 1 cut(s) 251
AluBI AGCT 8 cut(s) 90, 196, 255, 269, 391, 409, 538, 567
AluI AGCT 8 cut(s) 90, 196, 255, 269, 391, 409, 538, 567
Alw26I GTCTC 3 cut(s) 308, 390, 443
AlwNI CAGNNNCTG 1 cut(s) 226
AoxI GGCC 2 cut(s) 244, 551
ApeKI GCWGC 2 cut(s) 388, 564
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 551
AsuNHI GCTAGC 1 cut(s) 61
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 400, 576
BceAI ACGGC 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 308, 390, 443
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 562
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 2 cut(s) 389, 565
BlsI GCNGC 2 cut(s) 390, 566
BmgT120I GGNCC 1 cut(s) 551
BmsI GCATC 2 cut(s) 130, 340
BmtI GCTAGC 1 cut(s) 65
Bpu10I CCTNAGC 2 cut(s) 102, 294
BsaBI GATNNNNATC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 593
Bse1I ACTGG 2 cut(s) 493, 581
Bse3DI GCAATG 2 cut(s) 56, 66
Bse8I GATNNNNATC 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 2 cut(s) 121, 304
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 164, 593
BseMI GCAATG 2 cut(s) 56, 66
BseMII CTCAG 3 cut(s) 218, 285, 532
BseNI ACTGG 2 cut(s) 493, 581
BseRI GAGGAG 2 cut(s) 25, 28
BseXI GCAGC 2 cut(s) 400, 576
BsgI GTGCAG 1 cut(s) 256
BshFI GGCC 2 cut(s) 246, 553
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 164, 593
BsmAI GTCTC 3 cut(s) 308, 390, 443
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 2 cut(s) 246, 553
Bso31I GGTCTC 1 cut(s) 443
Bsp143I GATC 1 cut(s) 239
BspANI GGCC 2 cut(s) 246, 553
BspCNI CTCAG 3 cut(s) 217, 286, 531
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 461
BspOI GCTAGC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 2 cut(s) 56, 66
BsrI ACTGG 2 cut(s) 493, 581
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 478
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 4 cut(s) 102, 204, 294, 518
BstF5I GGATG 2 cut(s) 121, 304
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 3 cut(s) 308, 390, 443
BstMBI GATC 1 cut(s) 239
BstMWI GCNNNNNNNGC 4 cut(s) 243, 252, 350, 388
BstNSI RCATGY 1 cut(s) 288
BstSFI CTRYAG 1 cut(s) 562
BstV1I GCAGC 2 cut(s) 400, 576
BsuRI GGCC 2 cut(s) 246, 553
BtsCI GGATG 2 cut(s) 121, 304
BtsIMutI CAGTG 1 cut(s) 526
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 63
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 513, 541
CviAII CATG 2 cut(s) 43, 285
CviQI GTAC 2 cut(s) 513, 541
DdeI CTNAG 4 cut(s) 102, 204, 294, 518
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
DraIII CACNNNGTG 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 478
Eco147I AGGCCT 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 2 cut(s) 46, 288
FaiI YATR 7 cut(s) 44, 87, 146, 187, 189, 286, 332
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 446
FatI CATG 2 cut(s) 42, 284
Fnu4HI GCNGC 2 cut(s) 389, 565
FokI GGATG 2 cut(s) 108, 311
Fsp4HI GCNGC 2 cut(s) 389, 565
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 99
GluI GCNGC 2 cut(s) 389, 565
HaeIII GGCC 2 cut(s) 246, 553
HapII CCGG 1 cut(s) 93
HhaI GCGC 1 cut(s) 100
Hin1II CATG 2 cut(s) 46, 288
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 253
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 2 cut(s) 167, 207
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 257
HpyCH4IV ACGT 2 cut(s) 374, 471
HpyCH4V TGCA 6 cut(s) 237, 344, 353, 420, 456, 564
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 252, 350, 388
HpyF3I CTNAG 4 cut(s) 102, 204, 294, 518
HpySE526I ACGT 2 cut(s) 374, 471
Hsp92II CATG 2 cut(s) 46, 288
HspAI GCGC 1 cut(s) 98
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 2 cut(s) 95, 325
LpnPI CCDG 8 cut(s) 106, 192, 206, 212, 228, 466, 474, 594
Lsp1109I GCAGC 2 cut(s) 400, 576
LweI GCATC 2 cut(s) 130, 340
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 374, 471
MaeIII GTNAC 2 cut(s) 308, 359
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 3 cut(s) 43, 495, 550
MluCI AATT 3 cut(s) 152, 261, 483
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 9 cut(s) 22, 32, 43, 46, 49, 82, 213, 289, 527
MseI TTAA 3 cut(s) 264, 474, 601
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 243, 252, 350, 388
NdeII GATC 1 cut(s) 239
NheI GCTAGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 46, 288
NspI RCATGY 1 cut(s) 288
PceI AGGCCT 1 cut(s) 246
PciI ACATGT 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PkrI GCNGC 2 cut(s) 390, 566
PscI ACATGT 1 cut(s) 284
Psp1406I AACGTT 1 cut(s) 374
PspPI GGNCC 1 cut(s) 551
PstI CTGCAG 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 226
RsaI GTAC 2 cut(s) 514, 542
RsaNI GTAC 2 cut(s) 513, 541
SaqAI TTAA 3 cut(s) 264, 474, 601
SatI GCNGC 2 cut(s) 389, 565
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 551
SfaNI GCATC 2 cut(s) 130, 340
SfcI CTRYAG 1 cut(s) 562
Sse9I AATT 3 cut(s) 152, 261, 483
SseBI AGGCCT 1 cut(s) 246
SspMI CTAG 1 cut(s) 62
StuI AGGCCT 1 cut(s) 246
StyI CCWWGG 1 cut(s) 478
TaiI ACGT 2 cut(s) 377, 474
TaqI TCGA 1 cut(s) 432
TasI AATT 3 cut(s) 152, 261, 483
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 3 cut(s) 264, 474, 601
Tru9I TTAA 3 cut(s) 264, 474, 601
TscAI CASTG 1 cut(s) 526
TseI GCWGC 2 cut(s) 388, 564
TspDTI ATGAA 2 cut(s) 315, 355
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 1 cut(s) 526
XceI RCATGY 1 cut(s) 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.