Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005722

Physical Location & Seq

Genomic Coordinates Forward (+)

2027165 .. 2029013

Gene Structure 1849 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G04200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 573 bp

ATGGAAGAGAAGAAGACAGAATCCGCGACTAAGAACGTTAAAAAGGTTAACTTGTTAGACCACATCGCAATCAAACACCTCCTTGATGAATCCGTCTCCGATATCGTTACGAGCAGAGGGTACAAGGAGGATGTGAGATTAAGCAACGTCAAATTTCTGTTAGGAGGGATTATAATCGTGGTTGCTCTTGTTGCTCAATTCTACAACAAGAAATGTGGGATTTGTTTGCTTCTTGACTTTGGCAAGTATGTGGTGTTGACTGCGGTAATGCAGCTGATTCTGTACATTAAGGAGAAGAACGCTATCTTGTTCACCTATCCTCTTGAGGGATCATTCACCAGCACTGGTTTGGTGGTATCTTCCAAGTTACCTAGATTCTCTGATCAGTACACTCTAACCATCGACAGTGCTGATCCGCAATCAATCTCAGCTGGGAAGTCGGTTCAGTTCACCAAAAGTGTCACTCAATGGCTCACAAAAGATGGAGTTCTTGTCGAGGGTTTATTCTGGAAAGATGTTGAAGCCCTAGTCAAAGAGTACACAGAAGCAGGAGAACCAAAGAAGAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005787 GO:0005789 GO:0006465 GO:0006508 GO:0006518 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016485 GO:0019538 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0042175 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0098796 GO:0098827 GO:1901564 GO:1902494 GO:1905368

KEGG Pathways

Metabolic & Signaling

ko03060 map03060

Pfam Domains

Protein Families

SPC25

Protein Analysis

190

Amino Acids

21.23

Weight (kDa)

8.9

Isoelectric Point (pI)

25.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPC25	PF06703	23 - 177	5.8e-30	Microsomal signal peptidase 25 kDa subunit (SPC25)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005722)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39960 AT4G04200
fragaria_vesca	FvH4_7g24030
malus_domestica	MD07G1214300.v1.1 MD08G1058200.v1.1 MD15G1038100.v1.1
prunus_persica	Prupe.1G392100_v2.0.a1 Prupe.2G246200_v2.0.a1
pyrus_communis	pycom08g04830 pycom15g03530
rosa_chinensis	RchiOBHm_Chr1g0369411 RchiOBHm_Chr6g0299621
rosa_laevigata	RLG00000011404 RLG00000027132
rosa_multiflora	Rmu_sc0002895.1_g000003
rosa_roxburghii	Rroxscaffold_4G00287670 Rroxscaffold_7G00168500
rosa_rugosa	Rorug01G0349900 Rorug06G0291100
rosa_samantha	Rh1AG357500 Rh1BG321600 Rh1CG335400 Rh1DG352200 Rh6AG401900 Rh6CG415900 Rh6DG403100
rosa_wichuraiana	Rw1G031550 Rw6G035170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	173
AccII	CGCG	1 cut(s)	26
AciI	CCGC	3 cut(s)	24, 263, 416
AclI	AACGTT	1 cut(s)	36
AclWI	GGATC	2 cut(s)	337, 407
AcsI	RAATTY	1 cut(s)	152
AfaI	GTAC	4 cut(s)	122, 284, 389, 539
AfiI	CCNNNNNNNGG	1 cut(s)	326
AgsI	TTSAA	1 cut(s)	521
AluBI	AGCT	2 cut(s)	274, 431
AluI	AGCT	2 cut(s)	274, 431
Alw26I	GTCTC	1 cut(s)	100
AlwI	GGATC	2 cut(s)	337, 407
ApeKI	GCWGC	1 cut(s)	271
ApoI	RAATTY	1 cut(s)	152
AsuHPI	GGTGA	3 cut(s)	304, 328, 442
BbsI	GAAGAC	1 cut(s)	20
BbvI	GCAGC	1 cut(s)	283
BccI	CCATC	2 cut(s)	407, 476
BclI	TGATCA	1 cut(s)	382
BcoDI	GTCTC	1 cut(s)	100
BfaI	CTAG	2 cut(s)	372, 527
BisI	GCNGC	1 cut(s)	272
BlsI	GCNGC	1 cut(s)	273
BpiI	GAAGAC	1 cut(s)	20
BpuEI	CTTGAG	1 cut(s)	344
BsaBI	GATNNNNATC	1 cut(s)	173
BsaXI	ACNNNNNCTCC	2 cut(s)	477, 507
Bsc4I	CCNNNNNNNGG	1 cut(s)	326
Bse1I	ACTGG	1 cut(s)	349
Bse8I	GATNNNNATC	1 cut(s)	173
BseGI	GGATG	1 cut(s)	136
BseJI	GATNNNNATC	1 cut(s)	173
BseLI	CCNNNNNNNGG	1 cut(s)	326
BseMII	CTCAG	1 cut(s)	441
BseNI	ACTGG	1 cut(s)	349
BseXI	GCAGC	1 cut(s)	283
BseYI	CCCAGC	1 cut(s)	431
Bsh1236I	CGCG	1 cut(s)	26
BslI	CCNNNNNNNGG	1 cut(s)	326
BsmAI	GTCTC	1 cut(s)	100
BsmBI	CGTCTC	1 cut(s)	100
Bsp1407I	TGTACA	1 cut(s)	282
Bsp143I	GATC	3 cut(s)	329, 382, 412
BspACI	CCGC	3 cut(s)	24, 263, 416
BspCNI	CTCAG	1 cut(s)	440
BspFNI	CGCG	1 cut(s)	26
BspPI	GGATC	2 cut(s)	337, 407
BsrGI	TGTACA	1 cut(s)	282
BsrI	ACTGG	1 cut(s)	349
BssMI	GATC	3 cut(s)	329, 382, 412
Bst4CI	ACNGT	1 cut(s)	407
Bst6I	CTCTTC	1 cut(s)	560
BstAUI	TGTACA	1 cut(s)	282
BstDEI	CTNAG	2 cut(s)	30, 427
BstF5I	GGATG	1 cut(s)	136
BstFNI	CGCG	1 cut(s)	26
BstKTI	GATC	3 cut(s)	332, 385, 415
BstMAI	GTCTC	1 cut(s)	100
BstMBI	GATC	3 cut(s)	329, 382, 412
BstMWI	GCNNNNNNNGC	1 cut(s)	191
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	1 cut(s)	283
BstV2I	GAAGAC	1 cut(s)	20
BtgZI	GCGATG	1 cut(s)	49
BtsCI	GGATG	1 cut(s)	136
BtsIMutI	CAGTG	2 cut(s)	342, 412
Csp6I	GTAC	4 cut(s)	121, 283, 388, 538
CspCI	CAANNNNNGTGG	2 cut(s)	196, 231
CviJI	RGCY	4 cut(s)	274, 431, 472, 524
CviKI_1	RGCY	4 cut(s)	274, 431, 472, 524
CviQI	GTAC	4 cut(s)	121, 283, 388, 538
DdeI	CTNAG	2 cut(s)	30, 427
DpnI	GATC	3 cut(s)	331, 384, 414
DpnII	GATC	3 cut(s)	329, 382, 412
Eam1104I	CTCTTC	1 cut(s)	560
EarI	CTCTTC	1 cut(s)	560
Eco32I	GATATC	1 cut(s)	103
EcoRV	GATATC	1 cut(s)	103
Esp3I	CGTCTC	1 cut(s)	100
FaiI	YATR	2 cut(s)	173, 249
FalI	AAGNNNNNCTT	2 cut(s)	35, 67
FbaI	TGATCA	1 cut(s)	382
Fnu4HI	GCNGC	1 cut(s)	272
FokI	GGATG	1 cut(s)	143
Fsp4HI	GCNGC	1 cut(s)	272
FspBI	CTAG	2 cut(s)	372, 527
GluI	GCNGC	1 cut(s)	272
GsaI	CCCAGC	1 cut(s)	435
HincII	GTYRAC	2 cut(s)	49, 258
HindII	GTYRAC	2 cut(s)	49, 258
HinfI	GANTC	4 cut(s)	20, 89, 277, 375
HpaI	GTTAAC	1 cut(s)	49
HphI	GGTGA	3 cut(s)	304, 328, 442
Hpy166II	GTNNAC	6 cut(s)	49, 258, 312, 390, 450, 540
Hpy188I	TCNGA	2 cut(s)	100, 382
Hpy188III	TCNNGA	3 cut(s)	233, 323, 508
Hpy8I	GTNNAC	6 cut(s)	49, 258, 312, 390, 450, 540
HpyCH4III	ACNGT	1 cut(s)	407
HpyCH4IV	ACGT	2 cut(s)	36, 147
HpyCH4V	TGCA	1 cut(s)	271
HpyF10VI	GCNNNNNNNGC	1 cut(s)	191
HpyF3I	CTNAG	2 cut(s)	30, 427
HpySE526I	ACGT	2 cut(s)	36, 147
Ksp22I	TGATCA	1 cut(s)	382
KspAI	GTTAAC	1 cut(s)	49
Kzo9I	GATC	3 cut(s)	329, 382, 412
LpnPI	CCDG	5 cut(s)	330, 352, 417, 493, 534
Lsp1109I	GCAGC	1 cut(s)	283
MaeI	CTAG	2 cut(s)	372, 527
MaeII	ACGT	2 cut(s)	36, 147
MaeIII	GTNAC	3 cut(s)	106, 366, 460
MalI	GATC	3 cut(s)	331, 384, 414
MboI	GATC	3 cut(s)	329, 382, 412
MboII	GAAGA	5 cut(s)	17, 22, 25, 307, 351
MluCI	AATT	2 cut(s)	152, 197
MnlI	CCTC	7 cut(s)	89, 110, 121, 158, 319, 330, 490
MseI	TTAA	4 cut(s)	39, 48, 140, 288
MspA1I	CMGCKG	2 cut(s)	274, 431
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	1 cut(s)	191
NdeII	GATC	3 cut(s)	329, 382, 412
NmuCI	GTSAC	1 cut(s)	460
PfeI	GAWTC	4 cut(s)	20, 89, 277, 375
PkrI	GCNGC	1 cut(s)	273
PsiI	TTATAA	1 cut(s)	173
Psp1406I	AACGTT	1 cut(s)	36
PspFI	CCCAGC	1 cut(s)	431
PvuII	CAGCTG	2 cut(s)	274, 431
RsaI	GTAC	4 cut(s)	122, 284, 389, 539
RsaNI	GTAC	4 cut(s)	121, 283, 388, 538
SaqAI	TTAA	4 cut(s)	39, 48, 140, 288
SatI	GCNGC	1 cut(s)	272
Sau3AI	GATC	3 cut(s)	329, 382, 412
SetI	ASST	8 cut(s)	39, 48, 81, 150, 276, 317, 373, 433
SmlI	CTYRAG	1 cut(s)	323
SmoI	CTYRAG	1 cut(s)	323
Sse9I	AATT	2 cut(s)	152, 197
SsiI	CCGC	3 cut(s)	24, 263, 416
SspMI	CTAG	2 cut(s)	372, 527
TaaI	ACNGT	1 cut(s)	407
TaiI	ACGT	2 cut(s)	39, 150
TaqI	TCGA	2 cut(s)	402, 495
TasI	AATT	2 cut(s)	152, 197
TatI	WGTACW	3 cut(s)	282, 387, 537
TfiI	GAWTC	4 cut(s)	20, 89, 277, 375
Tru1I	TTAA	4 cut(s)	39, 48, 140, 288
Tru9I	TTAA	4 cut(s)	39, 48, 140, 288
TscAI	CASTG	2 cut(s)	349, 412
TseFI	GTSAC	1 cut(s)	460
TseI	GCWGC	1 cut(s)	271
Tsp45I	GTSAC	1 cut(s)	460
TspDTI	ATGAA	1 cut(s)	102
TspGWI	ACGGA	1 cut(s)	82
TspRI	CASTG	2 cut(s)	349, 412
XapI	RAATTY	1 cut(s)	152
XcmI	CCANNNNNNNNNTGG	1 cut(s)	346
XspI	CTAG	2 cut(s)	372, 527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.