Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005722

Physical Location & Seq

Genomic Coordinates Forward (+)

2660520 .. 2662756

Gene Structure 2237 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1038100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 588 bp

ATGTCGGAGACGAAGGCCGAGAGCAAGAACAAAAACCCTAAGAAGGTCAGTCTCTCAGATCACAGCTCCATCAAGCATCTCCTCGACGAGTCCGTCTCCGAGATTGTGAAGAGTCGCGGGTATGTGGAGAATACGAGGCTGAGCAACTTGAAGTTGTTGATTGGAACCGTCGTAATAATCATTGCTCTGTTTGCTCAGTTTTACAACAAGAAATTTCCGGAAAACCGAGATTTCCTGCTTGGATGCATTGCATTATATCCTTTTTCCTTGTATGTCATCGTTAATGGGATACTACAGGTGGTCTCGTACACGAAGGAGAAAAATGCAATCTTGTTCACTCATCCTCCTGCGGGAGCCTTTAGCACTGGGTTGGTGGTGTCTTCCAAGTTACCAAGATTTTCTGACTTGTACACGCTTACAGTAGCCAGTGCAGATCCGGAATCAATATCTGCAAACAAACCGGTGGAATTCACAAAGAGCGTTACTCAATGGTTTACTAAGGATGGAGTGTTGGTGGAAGGCCTTTTCTGGAAGGAAGTAGACGGACTGATAAACGATTACGCAAAAGAACCTAAGAAGAGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005787 GO:0005789 GO:0006465 GO:0006508 GO:0006518 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016485 GO:0019538 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0042175 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0098796 GO:0098827 GO:1901564 GO:1902494 GO:1905368

KEGG Pathways

Metabolic & Signaling

ko03060 map03060

Pfam Domains

Protein Families

SPC25

Protein Analysis

196

Amino Acids

21.74

Weight (kDa)

9.14

Isoelectric Point (pI)

28.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPC25	PF06703	24 - 185	1.3e-37	Microsomal signal peptidase 25 kDa subunit (SPC25)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005722)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39960 AT4G04200
fragaria_vesca	FvH4_7g24030
malus_domestica	MD07G1214300.v1.1 MD08G1058200.v1.1 MD15G1038100.v1.1
prunus_persica	Prupe.1G392100_v2.0.a1 Prupe.2G246200_v2.0.a1
pyrus_communis	pycom08g04830 pycom15g03530
rosa_chinensis	RchiOBHm_Chr1g0369411 RchiOBHm_Chr6g0299621
rosa_laevigata	RLG00000011404 RLG00000027132
rosa_multiflora	Rmu_sc0002895.1_g000003
rosa_roxburghii	Rroxscaffold_4G00287670 Rroxscaffold_7G00168500
rosa_rugosa	Rorug01G0349900 Rorug06G0291100
rosa_samantha	Rh1AG357500 Rh1BG321600 Rh1CG335400 Rh1DG352200 Rh6AG401900 Rh6CG415900 Rh6DG403100
rosa_wichuraiana	Rw1G031550 Rw6G035170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	92
AccI	GTMKAC	1 cut(s)	540
AccII	CGCG	1 cut(s)	117
AccIII	TCCGGA	2 cut(s)	217, 436
AciI	CCGC	2 cut(s)	117, 350
AclWI	GGATC	1 cut(s)	428
AcsI	RAATTY	2 cut(s)	212, 467
AfaI	GTAC	2 cut(s)	308, 410
AfiI	CCNNNNNNNGG	2 cut(s)	43, 350
AgeI	ACCGGT	1 cut(s)	460
AgsI	TTSAA	1 cut(s)	151
AluBI	AGCT	1 cut(s)	66
AluI	AGCT	1 cut(s)	66
Alw26I	GTCTC	4 cut(s)	2, 56, 100, 307
AlwI	GGATC	1 cut(s)	428
Aor13HI	TCCGGA	2 cut(s)	217, 436
AoxI	GGCC	2 cut(s)	15, 520
ApoI	RAATTY	2 cut(s)	212, 467
AsiGI	ACCGGT	1 cut(s)	460
BbsI	GAAGAC	1 cut(s)	372
BccI	CCATC	2 cut(s)	77, 497
BciVI	GTATCC	1 cut(s)	282
BcoDI	GTCTC	4 cut(s)	2, 56, 100, 307
BfmI	CTRYAG	1 cut(s)	293
BfuI	GTATCC	1 cut(s)	282
BlpI	GCTNAGC	1 cut(s)	140
BmiI	GGNNCC	2 cut(s)	166, 355
BmrI	ACTGGG	1 cut(s)	375
BmsI	GCATC	2 cut(s)	85, 233
BmuI	ACTGGG	1 cut(s)	375
BpiI	GAAGAC	1 cut(s)	372
BplI	GAGNNNNNCTC	4 cut(s)	80, 112, 469, 501
Bpu1102I	GCTNAGC	1 cut(s)	140
BsaI	GGTCTC	1 cut(s)	307
BsaWI	WCCGGW	3 cut(s)	217, 436, 460
BsaXI	ACNNNNNCTCC	4 cut(s)	328, 358, 498, 528
Bsc4I	CCNNNNNNNGG	2 cut(s)	43, 350
Bse118I	RCCGGY	1 cut(s)	460
Bse1I	ACTGG	2 cut(s)	370, 426
Bse3DI	GCAATG	2 cut(s)	180, 246
BseAI	TCCGGA	2 cut(s)	217, 436
BseGI	GGATG	3 cut(s)	248, 340, 508
BseLI	CCNNNNNNNGG	2 cut(s)	43, 350
BseMI	GCAATG	2 cut(s)	180, 246
BseMII	CTCAG	3 cut(s)	69, 131, 209
BseNI	ACTGG	2 cut(s)	370, 426
BseRI	GAGGAG	1 cut(s)	71
BsgI	GTGCAG	1 cut(s)	450
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	2 cut(s)	17, 522
BshTI	ACCGGT	1 cut(s)	460
BsiSI	CCGG	3 cut(s)	218, 437, 461
BslI	CCNNNNNNNGG	2 cut(s)	43, 350
BsmAI	GTCTC	4 cut(s)	2, 56, 100, 307
BsmBI	CGTCTC	2 cut(s)	2, 100
BsnI	GGCC	2 cut(s)	17, 522
Bso31I	GGTCTC	1 cut(s)	307
Bsp13I	TCCGGA	2 cut(s)	217, 436
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	2 cut(s)	58, 433
Bsp1720I	GCTNAGC	1 cut(s)	140
BspACI	CCGC	2 cut(s)	117, 350
BspANI	GGCC	2 cut(s)	17, 522
BspCNI	CTCAG	3 cut(s)	68, 132, 208
BspEI	TCCGGA	2 cut(s)	217, 436
BspFNI	CGCG	1 cut(s)	117
BspLI	GGNNCC	2 cut(s)	166, 355
BspPI	GGATC	1 cut(s)	428
BspQI	GCTCTTC	1 cut(s)	572
BspTNI	GGTCTC	1 cut(s)	307
BsrDI	GCAATG	2 cut(s)	180, 246
BsrFI	RCCGGY	1 cut(s)	460
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	2 cut(s)	370, 426
BssAI	RCCGGY	1 cut(s)	460
BssMI	GATC	2 cut(s)	58, 433
Bst4CI	ACNGT	2 cut(s)	169, 421
Bst6I	CTCTTC	2 cut(s)	104, 572
BstAUI	TGTACA	1 cut(s)	408
BstDEI	CTNAG	6 cut(s)	39, 55, 140, 195, 498, 573
BstF5I	GGATG	3 cut(s)	248, 340, 508
BstFNI	CGCG	1 cut(s)	117
BstKTI	GATC	2 cut(s)	61, 436
BstMAI	GTCTC	4 cut(s)	2, 56, 100, 307
BstMBI	GATC	2 cut(s)	58, 433
BstMWI	GCNNNNNNNGC	1 cut(s)	191
BstSFI	CTRYAG	1 cut(s)	293
BstUI	CGCG	1 cut(s)	117
BstV2I	GAAGAC	1 cut(s)	372
BstX2I	RGATCY	1 cut(s)	433
BstYI	RGATCY	1 cut(s)	433
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	2 cut(s)	17, 522
BtsCI	GGATG	3 cut(s)	248, 340, 508
BtsIMutI	CAGTG	2 cut(s)	363, 433
Cfr10I	RCCGGY	1 cut(s)	460
Csp6I	GTAC	2 cut(s)	307, 409
CspAI	ACCGGT	1 cut(s)	460
CviJI	RGCY	6 cut(s)	17, 66, 139, 356, 425, 522
CviKI_1	RGCY	6 cut(s)	17, 66, 139, 356, 425, 522
CviQI	GTAC	2 cut(s)	307, 409
DdeI	CTNAG	6 cut(s)	39, 55, 140, 195, 498, 573
DpnI	GATC	2 cut(s)	60, 435
DpnII	GATC	2 cut(s)	58, 433
DrdI	GACNNNNNNGTC	1 cut(s)	92
DseDI	GACNNNNNNGTC	1 cut(s)	92
Eam1104I	CTCTTC	2 cut(s)	104, 572
EarI	CTCTTC	2 cut(s)	104, 572
Eco147I	AGGCCT	1 cut(s)	522
Eco31I	GGTCTC	1 cut(s)	307
EcoRI	GAATTC	1 cut(s)	467
EcoT22I	ATGCAT	1 cut(s)	248
Esp3I	CGTCTC	2 cut(s)	2, 100
FaiI	YATR	3 cut(s)	123, 256, 273
FauI	CCCGC	2 cut(s)	110, 343
FblI	GTMKAC	1 cut(s)	540
FokI	GGATG	3 cut(s)	255, 327, 515
HaeIII	GGCC	2 cut(s)	17, 522
HapII	CCGG	3 cut(s)	218, 437, 461
HinfI	GANTC	3 cut(s)	89, 112, 440
HpaII	CCGG	3 cut(s)	218, 437, 461
Hpy166II	GTNNAC	5 cut(s)	309, 336, 411, 495, 541
Hpy188I	TCNGA	4 cut(s)	7, 58, 100, 403
Hpy188III	TCNNGA	3 cut(s)	218, 437, 529
Hpy8I	GTNNAC	5 cut(s)	309, 336, 411, 495, 541
Hpy99I	CGWCG	2 cut(s)	89, 173
HpyAV	CCTTC	5 cut(s)	7, 37, 307, 512, 526
HpyCH4III	ACNGT	2 cut(s)	169, 421
HpyCH4V	TGCA	5 cut(s)	246, 251, 326, 431, 452
HpyF10VI	GCNNNNNNNGC	1 cut(s)	191
HpyF3I	CTNAG	6 cut(s)	39, 55, 140, 195, 498, 573
Kpn2I	TCCGGA	2 cut(s)	217, 436
Kzo9I	GATC	2 cut(s)	58, 433
LguI	GCTCTTC	1 cut(s)	572
LmnI	GCTCC	2 cut(s)	71, 353
LpnPI	CCDG	9 cut(s)	231, 248, 281, 351, 360, 439, 450, 474, 514
LweI	GCATC	2 cut(s)	85, 233
MaeIII	GTNAC	2 cut(s)	387, 481
MalI	GATC	2 cut(s)	60, 435
MboI	GATC	2 cut(s)	58, 433
MboII	GAAGA	2 cut(s)	121, 372
MflI	RGATCY	1 cut(s)	433
MluCI	AATT	2 cut(s)	212, 467
MlyI	GAGTC	2 cut(s)	98, 121
MnlI	CCTC	3 cut(s)	92, 129, 354
Mph1103I	ATGCAT	1 cut(s)	248
MroI	TCCGGA	2 cut(s)	217, 436
MseI	TTAA	1 cut(s)	282
MspI	CCGG	3 cut(s)	218, 437, 461
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	1 cut(s)	191
NdeII	GATC	2 cut(s)	58, 433
NlaIV	GGNNCC	2 cut(s)	166, 355
NmeAIII	GCCGAG	1 cut(s)	43
NsiI	ATGCAT	1 cut(s)	248
PceI	AGGCCT	1 cut(s)	522
PciSI	GCTCTTC	1 cut(s)	572
PcsI	WCGNNNNNNNCGW	1 cut(s)	90
PfeI	GAWTC	1 cut(s)	440
PinAI	ACCGGT	1 cut(s)	460
PleI	GAGTC	2 cut(s)	97, 120
PpsI	GAGTC	2 cut(s)	97, 120
PspN4I	GGNNCC	2 cut(s)	166, 355
PsuI	RGATCY	1 cut(s)	433
RsaI	GTAC	2 cut(s)	308, 410
RsaNI	GTAC	2 cut(s)	307, 409
SapI	GCTCTTC	1 cut(s)	572
SaqAI	TTAA	1 cut(s)	282
Sau3AI	GATC	2 cut(s)	58, 433
SchI	GAGTC	2 cut(s)	98, 121
SetI	ASST	4 cut(s)	48, 68, 300, 574
SfaNI	GCATC	2 cut(s)	85, 233
SfcI	CTRYAG	1 cut(s)	293
Sse9I	AATT	2 cut(s)	212, 467
SseBI	AGGCCT	1 cut(s)	522
SsiI	CCGC	2 cut(s)	117, 350
StuI	AGGCCT	1 cut(s)	522
TaaI	ACNGT	2 cut(s)	169, 421
TaqI	TCGA	1 cut(s)	84
TasI	AATT	2 cut(s)	212, 467
TatI	WGTACW	1 cut(s)	408
TfiI	GAWTC	1 cut(s)	440
Tru1I	TTAA	1 cut(s)	282
Tru9I	TTAA	1 cut(s)	282
TscAI	CASTG	2 cut(s)	370, 433
TspGWI	ACGGA	2 cut(s)	82, 558
TspRI	CASTG	2 cut(s)	370, 433
XapI	RAATTY	2 cut(s)	212, 467
XmiI	GTMKAC	1 cut(s)	540
Zsp2I	ATGCAT	1 cut(s)	248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.