Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005722

Physical Location & Seq

Genomic Coordinates Reverse (-)

4656442 .. 4658269

Gene Structure 1828 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1058200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 366 bp

ATGATAATTCCGCAAGTCCGTTTCATCTTTGACTCTGGCAAGTATGTCATCGTTAATGGGATATTGCAGCTGGTCTCATACACGAAGGAGAAAAATGCAATCTTGTTCACTCATCCTCCTGCGGGGGCCTTTAGCACCGGGTTGGTGGTGTCTTCCAAATTACCAAGATTTTCTGACTTGTACACGCTTACAGTAGCCAGTGCAGATCCAGAATCAATATCTGCAAACAAACCGGTGGAATTCACAAAGAGCGTCACTCAGTGGTCTCTGTTTACCAAGGATGGAGTGTTGGTGGAAGGCCTTTTCTGGAAGGAAGTAGACGGACTCATCAACGAGTATGCAAAAGAACCGAAGAAAAGCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005787 GO:0005789 GO:0006465 GO:0006508 GO:0006518 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016485 GO:0019538 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0042175 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0098796 GO:0098827 GO:1901564 GO:1902494 GO:1905368

KEGG Pathways

Metabolic & Signaling

ko03060 map03060

Pfam Domains

Protein Families

SPC25

Protein Analysis

122

Amino Acids

13.43

Weight (kDa)

8.83

Isoelectric Point (pI)

24.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPC25	PF06703	15 - 111	2.5e-16	Microsomal signal peptidase 25 kDa subunit (SPC25)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0005722)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39960 AT4G04200
fragaria_vesca	FvH4_7g24030
malus_domestica	MD07G1214300.v1.1 MD08G1058200.v1.1 MD15G1038100.v1.1
prunus_persica	Prupe.1G392100_v2.0.a1 Prupe.2G246200_v2.0.a1
pyrus_communis	pycom08g04830 pycom15g03530
rosa_chinensis	RchiOBHm_Chr1g0369411 RchiOBHm_Chr6g0299621
rosa_laevigata	RLG00000011404 RLG00000027132
rosa_multiflora	Rmu_sc0002895.1_g000003
rosa_roxburghii	Rroxscaffold_4G00287670 Rroxscaffold_7G00168500
rosa_rugosa	Rorug01G0349900 Rorug06G0291100
rosa_samantha	Rh1AG357500 Rh1BG321600 Rh1CG335400 Rh1DG352200 Rh6AG401900 Rh6CG415900 Rh6DG403100
rosa_wichuraiana	Rw1G031550 Rw6G035170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	318
AciI	CCGC	2 cut(s)	11, 122
AclWI	GGATC	1 cut(s)	200
AcsI	RAATTY	1 cut(s)	239
AdeI	CACNNNGTG	1 cut(s)	261
AfaI	GTAC	1 cut(s)	182
AfiI	CCNNNNNNNGG	1 cut(s)	122
AgeI	ACCGGT	1 cut(s)	232
AluBI	AGCT	1 cut(s)	70
AluI	AGCT	1 cut(s)	70
Alw26I	GTCTC	2 cut(s)	79, 270
AlwI	GGATC	1 cut(s)	200
AoxI	GGCC	2 cut(s)	126, 298
ApeKI	GCWGC	1 cut(s)	67
ApoI	RAATTY	1 cut(s)	239
AsiGI	ACCGGT	1 cut(s)	232
AspS9I	GGNCC	1 cut(s)	126
AsuC2I	CCSGG	1 cut(s)	139
BbsI	GAAGAC	1 cut(s)	144
BbvI	GCAGC	1 cut(s)	79
BccI	CCATC	1 cut(s)	275
BcnI	CCSGG	1 cut(s)	139
BcoDI	GTCTC	2 cut(s)	79, 270
BisI	GCNGC	1 cut(s)	68
BlsI	GCNGC	1 cut(s)	69
Bme1390I	CCNGG	1 cut(s)	139
BmgT120I	GGNCC	1 cut(s)	126
BmiI	GGNNCC	1 cut(s)	127
BmrFI	CCNGG	1 cut(s)	139
BpiI	GAAGAC	1 cut(s)	144
BplI	GAGNNNNNCTC	2 cut(s)	241, 273
BpuMI	CCSGG	1 cut(s)	139
BsaI	GGTCTC	2 cut(s)	79, 270
BsaJI	CCNNGG	1 cut(s)	276
BsaWI	WCCGGW	1 cut(s)	232
BsaXI	ACNNNNNCTCC	4 cut(s)	100, 130, 276, 306
Bsc4I	CCNNNNNNNGG	1 cut(s)	122
Bse118I	RCCGGY	1 cut(s)	232
Bse1I	ACTGG	1 cut(s)	198
BseDI	CCNNGG	1 cut(s)	276
BseGI	GGATG	2 cut(s)	112, 286
BseLI	CCNNNNNNNGG	1 cut(s)	122
BseMII	CTCAG	1 cut(s)	272
BseNI	ACTGG	1 cut(s)	198
BseXI	GCAGC	1 cut(s)	79
BsgI	GTGCAG	1 cut(s)	222
BshFI	GGCC	2 cut(s)	128, 300
BshTI	ACCGGT	1 cut(s)	232
BsiSI	CCGG	2 cut(s)	138, 233
BslI	CCNNNNNNNGG	1 cut(s)	122
BsmAI	GTCTC	2 cut(s)	79, 270
BsnI	GGCC	2 cut(s)	128, 300
Bso31I	GGTCTC	2 cut(s)	79, 270
Bsp1407I	TGTACA	1 cut(s)	180
Bsp143I	GATC	1 cut(s)	205
BspACI	CCGC	2 cut(s)	11, 122
BspANI	GGCC	2 cut(s)	128, 300
BspCNI	CTCAG	1 cut(s)	271
BspLI	GGNNCC	1 cut(s)	127
BspPI	GGATC	1 cut(s)	200
BspTNI	GGTCTC	2 cut(s)	79, 270
BsrFI	RCCGGY	1 cut(s)	232
BsrGI	TGTACA	1 cut(s)	180
BsrI	ACTGG	1 cut(s)	198
BssAI	RCCGGY	1 cut(s)	232
BssECI	CCNNGG	1 cut(s)	276
BssMI	GATC	1 cut(s)	205
BssT1I	CCWWGG	1 cut(s)	276
Bst4CI	ACNGT	1 cut(s)	193
BstAUI	TGTACA	1 cut(s)	180
BstDEI	CTNAG	1 cut(s)	258
BstF5I	GGATG	2 cut(s)	112, 286
BstKTI	GATC	1 cut(s)	208
BstMAI	GTCTC	2 cut(s)	79, 270
BstMBI	GATC	1 cut(s)	205
BstSCI	CCNGG	1 cut(s)	137
BstV1I	GCAGC	1 cut(s)	79
BstV2I	GAAGAC	1 cut(s)	144
BstX2I	RGATCY	1 cut(s)	205
BstYI	RGATCY	1 cut(s)	205
BsuRI	GGCC	2 cut(s)	128, 300
BtsCI	GGATG	2 cut(s)	112, 286
BtsIMutI	CAGTG	2 cut(s)	205, 266
Cfr10I	RCCGGY	1 cut(s)	232
Cfr13I	GGNCC	1 cut(s)	126
CseI	GACGC	1 cut(s)	241
Csp6I	GTAC	1 cut(s)	181
CspAI	ACCGGT	1 cut(s)	232
CviJI	RGCY	4 cut(s)	70, 128, 197, 300
CviKI_1	RGCY	4 cut(s)	70, 128, 197, 300
CviQI	GTAC	1 cut(s)	181
DdeI	CTNAG	1 cut(s)	258
DpnI	GATC	1 cut(s)	207
DpnII	GATC	1 cut(s)	205
DraIII	CACNNNGTG	1 cut(s)	261
Eco130I	CCWWGG	1 cut(s)	276
Eco147I	AGGCCT	1 cut(s)	300
Eco31I	GGTCTC	2 cut(s)	79, 270
EcoO109I	RGGNCCY	1 cut(s)	126
EcoRI	GAATTC	1 cut(s)	239
EcoT14I	CCWWGG	1 cut(s)	276
ErhI	CCWWGG	1 cut(s)	276
FaiI	YATR	3 cut(s)	45, 79, 339
FauI	CCCGC	1 cut(s)	115
FblI	GTMKAC	1 cut(s)	318
Fnu4HI	GCNGC	1 cut(s)	68
FokI	GGATG	2 cut(s)	99, 293
Fsp4HI	GCNGC	1 cut(s)	68
GluI	GCNGC	1 cut(s)	68
HaeIII	GGCC	2 cut(s)	128, 300
HapII	CCGG	2 cut(s)	138, 233
HgaI	GACGC	1 cut(s)	241
HinfI	GANTC	3 cut(s)	32, 212, 324
HpaII	CCGG	2 cut(s)	138, 233
Hpy166II	GTNNAC	4 cut(s)	108, 183, 273, 319
Hpy188I	TCNGA	1 cut(s)	175
Hpy188III	TCNNGA	2 cut(s)	209, 307
Hpy8I	GTNNAC	4 cut(s)	108, 183, 273, 319
HpyAV	CCTTC	3 cut(s)	79, 290, 304
HpyCH4III	ACNGT	1 cut(s)	193
HpyCH4V	TGCA	5 cut(s)	67, 98, 203, 224, 341
HpyF3I	CTNAG	1 cut(s)	258
Kzo9I	GATC	1 cut(s)	205
LpnPI	CCDG	8 cut(s)	21, 56, 132, 151, 211, 222, 246, 292
Lsp1109I	GCAGC	1 cut(s)	79
MaeIII	GTNAC	1 cut(s)	253
MalI	GATC	1 cut(s)	207
MboI	GATC	1 cut(s)	205
MboII	GAAGA	2 cut(s)	144, 364
MflI	RGATCY	1 cut(s)	205
MluCI	AATT	3 cut(s)	6, 158, 239
MlyI	GAGTC	2 cut(s)	26, 318
MnlI	CCTC	1 cut(s)	126
MseI	TTAA	1 cut(s)	54
MspA1I	CMGCKG	1 cut(s)	70
MspI	CCGG	2 cut(s)	138, 233
MspR9I	CCNGG	1 cut(s)	139
NciI	CCSGG	1 cut(s)	139
NdeII	GATC	1 cut(s)	205
NlaIV	GGNNCC	1 cut(s)	127
NmuCI	GTSAC	1 cut(s)	253
PceI	AGGCCT	1 cut(s)	300
PfeI	GAWTC	1 cut(s)	212
PinAI	ACCGGT	1 cut(s)	232
PkrI	GCNGC	1 cut(s)	69
PleI	GAGTC	2 cut(s)	26, 318
PpsI	GAGTC	2 cut(s)	26, 318
PspN4I	GGNNCC	1 cut(s)	127
PspPI	GGNCC	1 cut(s)	126
PsuI	RGATCY	1 cut(s)	205
PvuII	CAGCTG	1 cut(s)	70
RsaI	GTAC	1 cut(s)	182
RsaNI	GTAC	1 cut(s)	181
SaqAI	TTAA	1 cut(s)	54
SatI	GCNGC	1 cut(s)	68
Sau3AI	GATC	1 cut(s)	205
Sau96I	GGNCC	1 cut(s)	126
SchI	GAGTC	2 cut(s)	26, 318
ScrFI	CCNGG	1 cut(s)	139
SetI	ASST	1 cut(s)	72
Sse9I	AATT	3 cut(s)	6, 158, 239
SseBI	AGGCCT	1 cut(s)	300
SsiI	CCGC	2 cut(s)	11, 122
StuI	AGGCCT	1 cut(s)	300
StyD4I	CCNGG	1 cut(s)	137
StyI	CCWWGG	1 cut(s)	276
TaaI	ACNGT	1 cut(s)	193
TasI	AATT	3 cut(s)	6, 158, 239
TatI	WGTACW	1 cut(s)	180
TfiI	GAWTC	1 cut(s)	212
Tru1I	TTAA	1 cut(s)	54
Tru9I	TTAA	1 cut(s)	54
TscAI	CASTG	2 cut(s)	205, 266
TseFI	GTSAC	1 cut(s)	253
TseI	GCWGC	1 cut(s)	67
Tsp45I	GTSAC	1 cut(s)	253
TspDTI	ATGAA	1 cut(s)	13
TspGWI	ACGGA	2 cut(s)	8, 336
TspRI	CASTG	2 cut(s)	205, 266
XapI	RAATTY	1 cut(s)	239
XmiI	GTMKAC	1 cut(s)	318

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.