C-5 cytosine-specific DNA methylase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G80740 AT4G19020 AT4G19020 |
| fragaria_vesca | FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 FvH4_5g23610 |
| malus_domestica | MD06G1022700.v1.1 |
| prunus_persica | Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 Prupe.5G024100_v2.0.a1 |
| pyrus_communis | pycom06g01820 pycom06g01830 pycom06g01850 pycom06g01860 |
| rosa_chinensis | RchiOBHm_Chr7g0212911 |
| rosa_laevigata | RLG00000002855 |
| rosa_multiflora | Rmu_co8277115.1_g000001 |
| rosa_roxburghii | Rroxscaffold_3G00246430 |
| rosa_rugosa | Rorug07G0137700 Rorug07G0137800 Rorug07G0137900 Rorug07G0138000 Rorug07G0138100 |
| rosa_samantha | Rh7AG269200 Rh7BG264800 Rh7CG288400 Rh7DG277400 |
| rosa_wichuraiana | Rw7G023390 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 3824 |
| Acc36I | ACCTGC | 1 cut(s) | 2323 |
| Acc65I | GGTACC | 2 cut(s) | 2125, 3505 |
| AccB1I | GGYRCC | 2 cut(s) | 2125, 3505 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2708 |
| AccI | GTMKAC | 1 cut(s) | 1725 |
| AccII | CGCG | 1 cut(s) | 3810 |
| AciI | CCGC | 6 cut(s) | 275, 419, 473, 2172, 3056, 3808 |
| AclI | AACGTT | 2 cut(s) | 666, 1142 |
| AcoI | YGGCCR | 2 cut(s) | 12, 1792 |
| AcsI | RAATTY | 4 cut(s) | 340, 484, 1342, 2446 |
| AcuI | CTGAAG | 6 cut(s) | 135, 234, 693, 1265, 2594, 3150 |
| AcyI | GRCGYC | 1 cut(s) | 1909 |
| AfaI | GTAC | 4 cut(s) | 2127, 2510, 3263, 3507 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 164, 515, 631, 1174, 1994, 2290, 2708, 2747, 3616 |
| AflII | CTTAAG | 1 cut(s) | 1628 |
| AgeI | ACCGGT | 1 cut(s) | 3475 |
| AhlI | ACTAGT | 4 cut(s) | 1220, 1292, 2462, 2675 |
| AjnI | CCWGG | 3 cut(s) | 624, 2707, 3508 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1773, 1805 |
| AleI | CACNNNNGTG | 1 cut(s) | 1029 |
| Alw21I | GWGCWC | 2 cut(s) | 556, 2589 |
| Alw26I | GTCTC | 8 cut(s) | 89, 767, 867, 1743, 1916, 2349, 3242, 3757 |
| Alw44I | GTGCAC | 1 cut(s) | 2585 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 277, 421, 581, 2501, 3296 |
| Ama87I | CYCGRG | 2 cut(s) | 1326, 3375 |
| ApaI | GGGCCC | 1 cut(s) | 331 |
| ApaLI | GTGCAC | 1 cut(s) | 2585 |
| ApeKI | GCWGC | 7 cut(s) | 122, 277, 421, 1307, 2294, 2348, 2472 |
| ApoI | RAATTY | 4 cut(s) | 340, 484, 1342, 2446 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2015, 2047 |
| AsiGI | ACCGGT | 1 cut(s) | 3475 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1465, 3579, 3876 |
| Asp718I | GGTACC | 2 cut(s) | 2125, 3505 |
| AspA2I | CCTAGG | 1 cut(s) | 1988 |
| AspLEI | GCGC | 1 cut(s) | 1614 |
| AspS9I | GGNCC | 5 cut(s) | 327, 328, 956, 2281, 2735 |
| AsuC2I | CCSGG | 4 cut(s) | 959, 1525, 3215, 3426 |
| AsuHPI | GGTGA | 6 cut(s) | 523, 994, 1067, 1781, 1977, 2555 |
| AsuII | TTCGAA | 1 cut(s) | 883 |
| AvaI | CYCGRG | 2 cut(s) | 1326, 3375 |
| AvaII | GGWCC | 2 cut(s) | 2281, 2735 |
| AvrII | CCTAGG | 1 cut(s) | 1988 |
| BaeGI | GKGCMC | 2 cut(s) | 331, 2589 |
| BalI | TGGCCA | 1 cut(s) | 1794 |
| BanI | GGYRCC | 2 cut(s) | 2125, 3505 |
| BanII | GRGCYC | 1 cut(s) | 331 |
| BauI | CACGAG | 3 cut(s) | 258, 402, 3762 |
| BbsI | GAAGAC | 4 cut(s) | 1374, 1683, 3216, 3369 |
| Bbv12I | GWGCWC | 2 cut(s) | 556, 2589 |
| BbvCI | CCTCAGC | 1 cut(s) | 3861 |
| BbvI | GCAGC | 7 cut(s) | 109, 264, 408, 1294, 2281, 2335, 2484 |
| BccI | CCATC | 9 cut(s) | 473, 625, 1052, 1602, 1817, 2328, 2860, 3472, 3496 |
| BceAI | ACGGC | 5 cut(s) | 1898, 2307, 2913, 3331, 3686 |
| BciT130I | CCWGG | 3 cut(s) | 626, 2709, 3510 |
| BciVI | GTATCC | 3 cut(s) | 1861, 2591, 3506 |
| BclI | TGATCA | 3 cut(s) | 2416, 2545, 3337 |
| BcnI | CCSGG | 4 cut(s) | 959, 1525, 3215, 3426 |
| BcoDI | GTCTC | 8 cut(s) | 89, 767, 867, 1743, 1916, 2349, 3242, 3757 |
| BcuI | ACTAGT | 4 cut(s) | 1220, 1292, 2462, 2675 |
| BfmI | CTRYAG | 1 cut(s) | 2130 |
| BfoI | RGCGCY | 1 cut(s) | 1615 |
| BfrI | CTTAAG | 1 cut(s) | 1628 |
| BfuAI | ACCTGC | 1 cut(s) | 2323 |
| BfuI | GTATCC | 3 cut(s) | 1861, 2591, 3506 |
| BglII | AGATCT | 6 cut(s) | 68, 788, 887, 1157, 2694, 3184 |
| BisI | GCNGC | 9 cut(s) | 123, 275, 278, 419, 422, 1308, 2295, 2349, 2473 |
| BlnI | CCTAGG | 1 cut(s) | 1988 |
| BlsI | GCNGC | 9 cut(s) | 124, 276, 279, 420, 423, 1309, 2296, 2350, 2474 |
| Bme1390I | CCNGG | 7 cut(s) | 626, 959, 1525, 2709, 3215, 3426, 3510 |
| Bme18I | GGWCC | 2 cut(s) | 2281, 2735 |
| BmeT110I | CYCGRG | 2 cut(s) | 1326, 3375 |
| BmgT120I | GGNCC | 5 cut(s) | 327, 328, 956, 2281, 2735 |
| BmiI | GGNNCC | 9 cut(s) | 329, 363, 507, 2127, 2239, 2736, 3474, 3507, 3709 |
| BmrFI | CCNGG | 7 cut(s) | 626, 959, 1525, 2709, 3215, 3426, 3510 |
| BmrI | ACTGGG | 1 cut(s) | 2232 |
| BmsI | GCATC | 9 cut(s) | 750, 878, 1116, 1252, 2335, 2644, 3163, 3637, 3826 |
| BmuI | ACTGGG | 1 cut(s) | 2232 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1418, 1532 |
| BpiI | GAAGAC | 4 cut(s) | 1374, 1683, 3216, 3369 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 775, 807, 2346, 2378 |
| BpmI | CTGGAG | 3 cut(s) | 647, 1636, 2373 |
| Bpu10I | CCTNAGC | 2 cut(s) | 3633, 3861 |
| Bpu14I | TTCGAA | 1 cut(s) | 883 |
| BpuEI | CTTGAG | 5 cut(s) | 21, 1584, 2095, 2126, 3119 |
| BpuMI | CCSGG | 4 cut(s) | 959, 1525, 3215, 3426 |
| BsaHI | GRCGYC | 1 cut(s) | 1909 |
| BsaJI | CCNNGG | 4 cut(s) | 1744, 1988, 2746, 3425 |
| BsaWI | WCCGGW | 4 cut(s) | 76, 364, 508, 3475 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 164, 515, 631, 1174, 1994, 2290, 2708, 2747, 3616 |
| Bse118I | RCCGGY | 3 cut(s) | 10, 2766, 3475 |
| Bse1I | ACTGG | 7 cut(s) | 520, 922, 1476, 1943, 2227, 2594, 3697 |
| Bse3DI | GCAATG | 1 cut(s) | 1272 |
| BseBI | CCWGG | 3 cut(s) | 626, 2709, 3510 |
| BseDI | CCNNGG | 4 cut(s) | 1744, 1988, 2746, 3425 |
| BseGI | GGATG | 8 cut(s) | 484, 864, 1070, 1183, 1675, 2320, 3577, 3628 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 164, 515, 631, 1174, 1994, 2290, 2708, 2747, 3616 |
| BseMI | GCAATG | 1 cut(s) | 1272 |
| BseMII | CTCAG | 6 cut(s) | 573, 588, 984, 2493, 3624, 3875 |
| BseNI | ACTGG | 7 cut(s) | 520, 922, 1476, 1943, 2227, 2594, 3697 |
| BseRI | GAGGAG | 6 cut(s) | 196, 240, 799, 1432, 1607, 2519 |
| BseSI | GKGCMC | 2 cut(s) | 331, 2589 |
| BseXI | GCAGC | 7 cut(s) | 109, 264, 408, 1294, 2281, 2335, 2484 |
| BsgI | GTGCAG | 2 cut(s) | 993, 3651 |
| Bsh1236I | CGCG | 1 cut(s) | 3810 |
| BshNI | GGYRCC | 2 cut(s) | 2125, 3505 |
| BshTI | ACCGGT | 1 cut(s) | 3475 |
| BsiHKAI | GWGCWC | 2 cut(s) | 556, 2589 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1326, 3375 |
| BslFI | GGGAC | 5 cut(s) | 90, 600, 1148, 2748, 3584 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 164, 515, 631, 1174, 1994, 2290, 2708, 2747, 3616 |
| BsmAI | GTCTC | 8 cut(s) | 89, 767, 867, 1743, 1916, 2349, 3242, 3757 |
| BsmBI | CGTCTC | 1 cut(s) | 1916 |
| BsmFI | GGGAC | 5 cut(s) | 90, 600, 1148, 2748, 3584 |
| BsmI | GAATGC | 2 cut(s) | 1706, 3847 |
| BsoBI | CYCGRG | 2 cut(s) | 1326, 3375 |
| Bsp119I | TTCGAA | 1 cut(s) | 883 |
| Bsp120I | GGGCCC | 1 cut(s) | 327 |
| Bsp1286I | GDGCHC | 3 cut(s) | 331, 556, 2589 |
| BspACI | CCGC | 6 cut(s) | 275, 419, 473, 2172, 3056, 3808 |
| BspCNI | CTCAG | 6 cut(s) | 574, 587, 985, 2494, 3625, 3874 |
| BspFNI | CGCG | 1 cut(s) | 3810 |
| BspHI | TCATGA | 4 cut(s) | 801, 1897, 2548, 2967 |
| BspLI | GGNNCC | 9 cut(s) | 329, 363, 507, 2127, 2239, 2736, 3474, 3507, 3709 |
| BspMI | ACCTGC | 1 cut(s) | 2323 |
| BspQI | GCTCTTC | 1 cut(s) | 2482 |
| BspT104I | TTCGAA | 1 cut(s) | 883 |
| BspT107I | GGYRCC | 2 cut(s) | 2125, 3505 |
| BspTI | CTTAAG | 1 cut(s) | 1628 |
| BsrDI | GCAATG | 1 cut(s) | 1272 |
| BsrFI | RCCGGY | 3 cut(s) | 10, 2766, 3475 |
| BsrI | ACTGG | 7 cut(s) | 520, 922, 1476, 1943, 2227, 2594, 3697 |
| BssAI | RCCGGY | 3 cut(s) | 10, 2766, 3475 |
| BssECI | CCNNGG | 4 cut(s) | 1744, 1988, 2746, 3425 |
| BssNI | GRCGYC | 1 cut(s) | 1909 |
| BssSI | CACGAG | 3 cut(s) | 258, 402, 3762 |
| BssT1I | CCWWGG | 3 cut(s) | 1744, 1988, 2746 |
| Bst2BI | CACGAG | 3 cut(s) | 258, 402, 3762 |
| Bst2UI | CCWGG | 3 cut(s) | 626, 2709, 3510 |
| Bst6I | CTCTTC | 5 cut(s) | 24, 210, 530, 1919, 2482 |
| BstACI | GRCGYC | 1 cut(s) | 1909 |
| BstAFI | CTTAAG | 1 cut(s) | 1628 |
| BstBI | TTCGAA | 1 cut(s) | 883 |
| BstC8I | GCNNGC | 6 cut(s) | 12, 336, 1716, 1887, 2396, 3808 |
| BstDEI | CTNAG | 7 cut(s) | 574, 582, 993, 1644, 2502, 3633, 3861 |
| BstEII | GGTNACC | 2 cut(s) | 367, 1519 |
| BstF5I | GGATG | 8 cut(s) | 484, 864, 1070, 1183, 1675, 2320, 3577, 3628 |
| BstFNI | CGCG | 1 cut(s) | 3810 |
| BstH2I | RGCGCY | 1 cut(s) | 1615 |
| BstHHI | GCGC | 1 cut(s) | 1614 |
| BstMAI | GTCTC | 8 cut(s) | 89, 767, 867, 1743, 1916, 2349, 3242, 3757 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 335, 614, 1304, 2185, 3064, 3634 |
| BstNI | CCWGG | 3 cut(s) | 626, 2709, 3510 |
| BstNSI | RCATGY | 3 cut(s) | 338, 1340, 3609 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1418, 1532 |
| BstPI | GGTNACC | 2 cut(s) | 367, 1519 |
| BstSCI | CCNGG | 7 cut(s) | 624, 957, 1523, 2707, 3213, 3424, 3508 |
| BstSFI | CTRYAG | 1 cut(s) | 2130 |
| BstSLI | GKGCMC | 2 cut(s) | 331, 2589 |
| BstUI | CGCG | 1 cut(s) | 3810 |
| BstV1I | GCAGC | 7 cut(s) | 109, 264, 408, 1294, 2281, 2335, 2484 |
| BstV2I | GAAGAC | 4 cut(s) | 1374, 1683, 3216, 3369 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 625, 3704 |
| BsuI | GTATCC | 3 cut(s) | 1861, 2591, 3506 |
| BtgZI | GCGATG | 1 cut(s) | 1386 |
| BtsCI | GGATG | 8 cut(s) | 484, 864, 1070, 1183, 1675, 2320, 3577, 3628 |
| BtsI | GCAGTG | 1 cut(s) | 3750 |
| BtsIMutI | CAGTG | 8 cut(s) | 499, 1024, 1469, 1853, 2139, 2206, 2587, 3750 |
| BveI | ACCTGC | 1 cut(s) | 2323 |
| Cac8I | GCNNGC | 6 cut(s) | 12, 336, 1716, 1887, 2396, 3808 |
| CaiI | CAGNNNCTG | 5 cut(s) | 277, 421, 581, 2501, 3296 |
| CciI | TCATGA | 4 cut(s) | 801, 1897, 2548, 2967 |
| CfoI | GCGC | 1 cut(s) | 1614 |
| Cfr10I | RCCGGY | 3 cut(s) | 10, 2766, 3475 |
| Cfr13I | GGNCC | 5 cut(s) | 327, 328, 956, 2281, 2735 |
| CseI | GACGC | 2 cut(s) | 202, 1898 |
| Csp6I | GTAC | 4 cut(s) | 2126, 2509, 3262, 3506 |
| CspAI | ACCGGT | 1 cut(s) | 3475 |
| CviQI | GTAC | 4 cut(s) | 2126, 2509, 3262, 3506 |
| DdeI | CTNAG | 7 cut(s) | 574, 582, 993, 1644, 2502, 3633, 3861 |
| DraI | TTTAAA | 1 cut(s) | 1398 |
| EaeI | YGGCCR | 2 cut(s) | 12, 1792 |
| Eam1104I | CTCTTC | 5 cut(s) | 24, 210, 530, 1919, 2482 |
| EarI | CTCTTC | 5 cut(s) | 24, 210, 530, 1919, 2482 |
| Eco130I | CCWWGG | 3 cut(s) | 1744, 1988, 2746 |
| Eco24I | GRGCYC | 1 cut(s) | 331 |
| Eco32I | GATATC | 1 cut(s) | 3180 |
| Eco47I | GGWCC | 2 cut(s) | 2281, 2735 |
| Eco57I | CTGAAG | 6 cut(s) | 135, 234, 693, 1265, 2594, 3150 |
| Eco88I | CYCGRG | 2 cut(s) | 1326, 3375 |
| Eco91I | GGTNACC | 2 cut(s) | 367, 1519 |
| EcoO109I | RGGNCCY | 3 cut(s) | 327, 328, 2735 |
| EcoO65I | GGTNACC | 2 cut(s) | 367, 1519 |
| EcoRII | CCWGG | 3 cut(s) | 624, 2707, 3508 |
| EcoRV | GATATC | 1 cut(s) | 3180 |
| EcoT14I | CCWWGG | 3 cut(s) | 1744, 1988, 2746 |
| EcoT22I | ATGCAT | 1 cut(s) | 1338 |
| EcoT38I | GRGCYC | 1 cut(s) | 331 |
| ErhI | CCWWGG | 3 cut(s) | 1744, 1988, 2746 |
| Esp3I | CGTCTC | 1 cut(s) | 1916 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2461, 2493, 2892, 2924 |
| FaqI | GGGAC | 5 cut(s) | 90, 600, 1148, 2748, 3584 |
| FauI | CCCGC | 1 cut(s) | 3815 |
| FauNDI | CATATG | 1 cut(s) | 2857 |
| FbaI | TGATCA | 3 cut(s) | 2416, 2545, 3337 |
| FblI | GTMKAC | 1 cut(s) | 1725 |
| Fnu4HI | GCNGC | 9 cut(s) | 123, 275, 278, 419, 422, 1308, 2295, 2349, 2473 |
| FokI | GGATG | 8 cut(s) | 491, 871, 1057, 1190, 1682, 2307, 3584, 3615 |
| FriOI | GRGCYC | 1 cut(s) | 331 |
| Fsp4HI | GCNGC | 9 cut(s) | 123, 275, 278, 419, 422, 1308, 2295, 2349, 2473 |
| GlaI | GCGC | 1 cut(s) | 1613 |
| GluI | GCNGC | 9 cut(s) | 123, 275, 278, 419, 422, 1308, 2295, 2349, 2473 |
| GsuI | CTGGAG | 3 cut(s) | 647, 1636, 2373 |
| HaeII | RGCGCY | 1 cut(s) | 1615 |
| HgaI | GACGC | 2 cut(s) | 202, 1898 |
| HhaI | GCGC | 1 cut(s) | 1614 |
| Hin1I | GRCGYC | 1 cut(s) | 1909 |
| Hin6I | GCGC | 1 cut(s) | 1612 |
| HinP1I | GCGC | 1 cut(s) | 1612 |
| HincII | GTYRAC | 3 cut(s) | 288, 1726, 2220 |
| HindII | GTYRAC | 3 cut(s) | 288, 1726, 2220 |
| HindIII | AAGCTT | 1 cut(s) | 2102 |
| HphI | GGTGA | 6 cut(s) | 523, 994, 1067, 1781, 1977, 2555 |
| Hpy166II | GTNNAC | 7 cut(s) | 288, 1514, 1726, 2220, 2281, 2587, 2938 |
| Hpy8I | GTNNAC | 7 cut(s) | 288, 1514, 1726, 2220, 2281, 2587, 2938 |
| Hpy99I | CGWCG | 4 cut(s) | 196, 1730, 2612, 3815 |
| HpyCH4IV | ACGT | 6 cut(s) | 666, 1142, 1552, 2090, 3280, 3516 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 335, 614, 1304, 2185, 3064, 3634 |
| HpyF3I | CTNAG | 7 cut(s) | 574, 582, 993, 1644, 2502, 3633, 3861 |
| HpySE526I | ACGT | 6 cut(s) | 666, 1142, 1552, 2090, 3280, 3516 |
| Hsp92I | GRCGYC | 1 cut(s) | 1909 |
| HspAI | GCGC | 1 cut(s) | 1612 |
| KpnI | GGTACC | 2 cut(s) | 2129, 3509 |
| KroI | GCCGGC | 1 cut(s) | 10 |
| KroNI | GCCGGC | 1 cut(s) | 12 |
| Ksp22I | TGATCA | 3 cut(s) | 2416, 2545, 3337 |
| LguI | GCTCTTC | 1 cut(s) | 2482 |
| LmnI | GCTCC | 7 cut(s) | 46, 130, 159, 1255, 2237, 3023, 3404 |
| Lsp1109I | GCAGC | 7 cut(s) | 109, 264, 408, 1294, 2281, 2335, 2484 |
| LweI | GCATC | 9 cut(s) | 750, 878, 1116, 1252, 2335, 2644, 3163, 3637, 3826 |
| MaeII | ACGT | 6 cut(s) | 666, 1142, 1552, 2090, 3280, 3516 |
| MfeI | CAATTG | 1 cut(s) | 3300 |
| MhlI | GDGCHC | 3 cut(s) | 331, 556, 2589 |
| MlsI | TGGCCA | 1 cut(s) | 1794 |
| MluNI | TGGCCA | 1 cut(s) | 1794 |
| MlyI | GAGTC | 3 cut(s) | 32, 769, 1280 |
| MmeI | TCCRAC | 5 cut(s) | 1511, 1975, 2389, 3086, 3608 |
| Mox20I | TGGCCA | 1 cut(s) | 1794 |
| Mph1103I | ATGCAT | 1 cut(s) | 1338 |
| MroNI | GCCGGC | 1 cut(s) | 10 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1465, 3579, 3876 |
| MscI | TGGCCA | 1 cut(s) | 1794 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1029, 2775, 3357 |
| Msp20I | TGGCCA | 1 cut(s) | 1794 |
| MspA1I | CMGCKG | 3 cut(s) | 277, 421, 2975 |
| MspCI | CTTAAG | 1 cut(s) | 1628 |
| MspR9I | CCNGG | 7 cut(s) | 626, 959, 1525, 2709, 3215, 3426, 3510 |
| MunI | CAATTG | 1 cut(s) | 3300 |
| Mva1269I | GAATGC | 2 cut(s) | 1706, 3847 |
| MvaI | CCWGG | 3 cut(s) | 626, 2709, 3510 |
| MvnI | CGCG | 1 cut(s) | 3810 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 335, 614, 1304, 2185, 3064, 3634 |
| NaeI | GCCGGC | 1 cut(s) | 12 |
| NciI | CCSGG | 4 cut(s) | 959, 1525, 3215, 3426 |
| NdeI | CATATG | 1 cut(s) | 2857 |
| NgoMIV | GCCGGC | 1 cut(s) | 10 |
| NlaIV | GGNNCC | 9 cut(s) | 329, 363, 507, 2127, 2239, 2736, 3474, 3507, 3709 |
| NmeAIII | GCCGAG | 2 cut(s) | 342, 486 |
| NmuCI | GTSAC | 5 cut(s) | 267, 411, 511, 1730, 2263 |
| NsiI | ATGCAT | 1 cut(s) | 1338 |
| NspI | RCATGY | 3 cut(s) | 338, 1340, 3609 |
| NspV | TTCGAA | 1 cut(s) | 883 |
| OliI | CACNNNNGTG | 1 cut(s) | 1029 |
| PaeI | GCATGC | 1 cut(s) | 338 |
| PagI | TCATGA | 4 cut(s) | 801, 1897, 2548, 2967 |
| PciSI | GCTCTTC | 1 cut(s) | 2482 |
| PctI | GAATGC | 2 cut(s) | 1706, 3847 |
| PdiI | GCCGGC | 1 cut(s) | 12 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1465, 3579, 3876 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2708 |
| PinAI | ACCGGT | 1 cut(s) | 3475 |
| PkrI | GCNGC | 9 cut(s) | 124, 276, 279, 420, 423, 1309, 2296, 2350, 2474 |
| PleI | GAGTC | 3 cut(s) | 31, 768, 1279 |
| PpsI | GAGTC | 3 cut(s) | 31, 768, 1279 |
| PpuMI | RGGWCCY | 1 cut(s) | 2735 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1418, 1532 |
| PsiI | TTATAA | 1 cut(s) | 3824 |
| Psp1406I | AACGTT | 2 cut(s) | 666, 1142 |
| Psp5II | RGGWCCY | 1 cut(s) | 2735 |
| Psp6I | CCWGG | 3 cut(s) | 624, 2707, 3508 |
| PspEI | GGTNACC | 2 cut(s) | 367, 1519 |
| PspGI | CCWGG | 3 cut(s) | 624, 2707, 3508 |
| PspN4I | GGNNCC | 9 cut(s) | 329, 363, 507, 2127, 2239, 2736, 3474, 3507, 3709 |
| PspOMI | GGGCCC | 1 cut(s) | 327 |
| PspPI | GGNCC | 5 cut(s) | 327, 328, 956, 2281, 2735 |
| PspPPI | RGGWCCY | 1 cut(s) | 2735 |
| PsrI | GAACNNNNNNTAC | 6 cut(s) | 345, 377, 489, 521, 2930, 2962 |
| PstNI | CAGNNNCTG | 5 cut(s) | 277, 421, 581, 2501, 3296 |
| PvuII | CAGCTG | 1 cut(s) | 2975 |
| RsaI | GTAC | 4 cut(s) | 2127, 2510, 3263, 3507 |
| RsaNI | GTAC | 4 cut(s) | 2126, 2509, 3262, 3506 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1029, 2775, 3357 |
| SalI | GTCGAC | 1 cut(s) | 1724 |
| SapI | GCTCTTC | 1 cut(s) | 2482 |
| SatI | GCNGC | 9 cut(s) | 123, 275, 278, 419, 422, 1308, 2295, 2349, 2473 |
| Sau96I | GGNCC | 5 cut(s) | 327, 328, 956, 2281, 2735 |
| SchI | GAGTC | 3 cut(s) | 32, 769, 1280 |
| ScrFI | CCNGG | 7 cut(s) | 626, 959, 1525, 2709, 3215, 3426, 3510 |
| SduI | GDGCHC | 3 cut(s) | 331, 556, 2589 |
| SfaNI | GCATC | 9 cut(s) | 750, 878, 1116, 1252, 2335, 2644, 3163, 3637, 3826 |
| SfcI | CTRYAG | 1 cut(s) | 2130 |
| SfuI | TTCGAA | 1 cut(s) | 883 |
| SinI | GGWCC | 2 cut(s) | 2281, 2735 |
| SmiI | ATTTAAAT | 1 cut(s) | 1398 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1029, 2775, 3357 |
| SmlI | CTYRAG | 6 cut(s) | 36, 1599, 1628, 2074, 2105, 3098 |
| SmoI | CTYRAG | 6 cut(s) | 36, 1599, 1628, 2074, 2105, 3098 |
| SpeI | ACTAGT | 4 cut(s) | 1220, 1292, 2462, 2675 |
| SphI | GCATGC | 1 cut(s) | 338 |
| SsiI | CCGC | 6 cut(s) | 275, 419, 473, 2172, 3056, 3808 |
| SspI | AATATT | 1 cut(s) | 1394 |
| StyD4I | CCNGG | 7 cut(s) | 624, 957, 1523, 2707, 3213, 3424, 3508 |
| StyI | CCWWGG | 3 cut(s) | 1744, 1988, 2746 |
| SwaI | ATTTAAAT | 1 cut(s) | 1398 |
| TaiI | ACGT | 6 cut(s) | 669, 1145, 1555, 2093, 3283, 3519 |
| TaqI | TCGA | 9 cut(s) | 345, 530, 558, 883, 1422, 1725, 3001, 3105, 3643 |
| TauI | GCSGC | 2 cut(s) | 277, 421 |
| TscAI | CASTG | 8 cut(s) | 499, 1031, 1476, 1853, 2146, 2206, 2594, 3750 |
| TseFI | GTSAC | 5 cut(s) | 267, 411, 511, 1730, 2263 |
| TseI | GCWGC | 7 cut(s) | 122, 277, 421, 1307, 2294, 2348, 2472 |
| Tsp45I | GTSAC | 5 cut(s) | 267, 411, 511, 1730, 2263 |
| TspGWI | ACGGA | 8 cut(s) | 117, 180, 237, 827, 1190, 1880, 2431, 3039 |
| TspRI | CASTG | 8 cut(s) | 499, 1031, 1476, 1853, 2146, 2206, 2594, 3750 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2708 |
| Vha464I | CTTAAG | 1 cut(s) | 1628 |
| VneI | GTGCAC | 1 cut(s) | 2585 |
| VpaK11BI | GGWCC | 2 cut(s) | 2281, 2735 |
| XapI | RAATTY | 4 cut(s) | 340, 484, 1342, 2446 |
| XbaI | TCTAGA | 1 cut(s) | 65 |
| XceI | RCATGY | 3 cut(s) | 338, 1340, 3609 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2753 |
| XmaJI | CCTAGG | 1 cut(s) | 1988 |
| XmiI | GTMKAC | 1 cut(s) | 1725 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1465, 3579, 3876 |
| Zsp2I | ATGCAT | 1 cut(s) | 1338 |