AT4G20300

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006362
Physical Location & Seq
Forward (+)
10955311 .. 10959239
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20300.2

Sequence Viewer

Length: 1020 bp
ATGATGAGGTATCAGAGAGTAAGCCCAGATTGTCTTCCTCTCACAAATGGAGGTAAGAAGCCTTACTTACGACCATCACCATCAAGAGCAACAAACGAAGACACCACCACCACCACCGTCATTACCACAACCTCAATCGCCGGAAGAGGATTTAACGGCGGATCATGCACAACCACCACCAACAACACCTCATCTCTAGACGGAGTCCCCAAAGGATTCCGATTCAGATCCACTCAACAGCAGCAACAACAACAAGATCCTAGTCCTAGCCGTCGCGGCGGTGATGTACTGTTACAGTGGGGACAACGAAAACGATCGAGAGCCTCTAGAGCTGAGATCCGATCCACCACCACCATCACAACCACCGCCGATGATTCTTCGTCTTCTTCAGGTCAAGGTAAGATACAATCAAACAAACCGCAAAGAAGATCGATGAATCCTTCTATGCCACCTCCACCACCAGCTCCGCCGATTTTCTCGGGCCGGAGTACAAACCCTAGAAACGGTTTCGTTATCGGCAAGGAATCTTTCTTCCCTTCCAGGAATCTAGAAGATCGATCAGCAAACGGATCACCATCAAGGAACAACATAAACGGCCGTATGATATCCAGATCGGGTGGCTCAAAGCGATCTCCACCGTCACCAGACCAAATCGAGAAGAGATCAAGCGTTAGAGATCAGAGACAAAACGGGTTTGATCATCAACAACAACAACATCAAAGAGTGAACAGATCAGAATCAACGGCTCAGGGTCATCAAGAAGTGGAGATAAATGGAGAAAGAGAGAAAGCAACGCAAGAGTGGCCGAGGATCTACATTGCTTTGTCTAGGAAAGAGAAGGAAGAAGATTTCTTGGTTATGAAAGGAACTAAGCTTCCTCATAGACCCAGGAAACGAGCTAAGAACATTGATAAAGCCCTTCAGTTTTGTTTTCCAGGGATGTGGTTATCTGATTTGACCAAGAACCGTTATGAAGTTAGGGAGAAGAAAAATGTGAAGAAGGTAATTAACTTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

339

Amino Acids

38.13

Weight (kDa)

10.83

Isoelectric Point (pI)

74.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 275 - 324 8.3e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 276
AciI CCGC 6 cut(s) 159, 276, 279, 366, 419, 467
AclWI GGATC 7 cut(s) 169, 222, 251, 331, 336, 577, 818
AcoI YGGCCR 2 cut(s) 595, 803
AcuI CTGAAG 2 cut(s) 372, 905
AfaI GTAC 2 cut(s) 288, 490
AfiI CCNNNNNNNGG 1 cut(s) 503
AjnI CCWGG 3 cut(s) 539, 887, 934
AloI GAACNNNNNNTCC 2 cut(s) 859, 891
AluBI AGCT 4 cut(s) 332, 464, 874, 899
AluI AGCT 4 cut(s) 332, 464, 874, 899
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 7 cut(s) 169, 222, 251, 331, 336, 577, 818
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 3 cut(s) 481, 595, 803
ApeKI GCWGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 481
AsuHPI GGTGA 4 cut(s) 69, 293, 564, 633
AvaI CYCGRG 1 cut(s) 478
BbsI GAAGAC 3 cut(s) 26, 105, 375
BbvI GCAGC 1 cut(s) 253
BccI CCATC 4 cut(s) 82, 88, 362, 583
BceAI ACGGC 5 cut(s) 172, 255, 582, 610, 759
BciT130I CCWGG 3 cut(s) 541, 889, 936
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 7 cut(s) 197, 261, 267, 327, 498, 548, 828
BglI GCCNNNNNGGC 1 cut(s) 276
BisI GCNGC 2 cut(s) 242, 277
BlsI GCNGC 2 cut(s) 243, 278
Bme1390I CCNGG 3 cut(s) 541, 889, 936
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 481
BmrFI CCNGG 3 cut(s) 541, 889, 936
BpiI GAAGAC 3 cut(s) 26, 105, 375
Bpu10I CCTNAGC 1 cut(s) 747
Bsa29I ATCGAT 2 cut(s) 431, 556
BsaBI GATNNNNATC 3 cut(s) 226, 574, 736
BsaJI CCNNGG 3 cut(s) 806, 887, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse3DI GCAATG 1 cut(s) 816
Bse8I GATNNNNATC 3 cut(s) 226, 574, 736
BseBI CCWGG 3 cut(s) 541, 889, 936
BseCI ATCGAT 2 cut(s) 431, 556
BseDI CCNNGG 3 cut(s) 806, 887, 935
BseGI GGATG 1 cut(s) 945
BseJI GATNNNNATC 3 cut(s) 226, 574, 736
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 2 cut(s) 324, 761
BseX3I CGGCCG 1 cut(s) 595
BseXI GCAGC 1 cut(s) 253
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 2 cut(s) 317, 598
BshFI GGCC 3 cut(s) 483, 597, 805
BshVI ATCGAT 2 cut(s) 431, 556
BsiEI CGRYCG 2 cut(s) 317, 598
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 2 cut(s) 141, 484
BslFI GGGAC 2 cut(s) 191, 315
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 2 cut(s) 191, 315
BsnI GGCC 3 cut(s) 483, 597, 805
BsoBI CYCGRG 1 cut(s) 478
BspACI CCGC 6 cut(s) 159, 276, 279, 366, 419, 467
BspANI GGCC 3 cut(s) 483, 597, 805
BspCNI CTCAG 2 cut(s) 325, 760
BspDI ATCGAT 2 cut(s) 431, 556
BspFNI CGCG 1 cut(s) 276
BspPI GGATC 7 cut(s) 169, 222, 251, 331, 336, 577, 818
BsrDI GCAATG 1 cut(s) 816
BssECI CCNNGG 3 cut(s) 806, 887, 935
Bst2UI CCWGG 3 cut(s) 541, 889, 936
Bst4CI ACNGT 6 cut(s) 118, 291, 297, 506, 639, 968
Bst6I CTCTTC 2 cut(s) 139, 653
BstDEI CTNAG 4 cut(s) 333, 747, 870, 900
BstF5I GGATG 1 cut(s) 945
BstFNI CGCG 1 cut(s) 276
BstMAI GTCTC 1 cut(s) 676
BstMCI CGRYCG 2 cut(s) 317, 598
BstMWI GCNNNNNNNGC 4 cut(s) 165, 276, 329, 802
BstNI CCWGG 3 cut(s) 541, 889, 936
BstSCI CCNGG 3 cut(s) 539, 887, 934
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 3 cut(s) 26, 105, 375
BstX2I RGATCY 4 cut(s) 227, 256, 336, 810
BstXI CCANNNNNNTGG 1 cut(s) 942
BstYI RGATCY 4 cut(s) 227, 256, 336, 810
BstZI CGGCCG 1 cut(s) 595
Bsu15I ATCGAT 2 cut(s) 431, 556
BsuRI GGCC 3 cut(s) 483, 597, 805
BsuTUI ATCGAT 2 cut(s) 431, 556
BtsCI GGATG 1 cut(s) 945
BtsIMutI CAGTG 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 481
ClaI ATCGAT 2 cut(s) 431, 556
Csp6I GTAC 2 cut(s) 287, 489
CviAII CATG 1 cut(s) 165
CviQI GTAC 2 cut(s) 287, 489
DdeI CTNAG 4 cut(s) 333, 747, 870, 900
EaeI YGGCCR 2 cut(s) 595, 803
EagI CGGCCG 1 cut(s) 595
Eam1104I CTCTTC 2 cut(s) 139, 653
EarI CTCTTC 2 cut(s) 139, 653
EciI GGCGGA 2 cut(s) 174, 456
EclXI CGGCCG 1 cut(s) 595
Eco32I GATATC 1 cut(s) 606
Eco52I CGGCCG 1 cut(s) 595
Eco57I CTGAAG 2 cut(s) 372, 905
Eco88I CYCGRG 1 cut(s) 478
EcoRII CCWGG 3 cut(s) 539, 887, 934
EcoRV GATATC 1 cut(s) 606
FaeI CATG 1 cut(s) 168
FaiI YATR 7 cut(s) 166, 446, 590, 602, 860, 882, 972
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 2 cut(s) 191, 315
FatI CATG 1 cut(s) 164
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 242, 277
FokI GGATG 1 cut(s) 952
Fsp4HI GCNGC 2 cut(s) 242, 277
FspBI CTAG 7 cut(s) 197, 261, 267, 327, 498, 548, 828
GluI GCNGC 2 cut(s) 242, 277
HaeIII GGCC 3 cut(s) 483, 597, 805
HapII CCGG 2 cut(s) 141, 484
Hin1II CATG 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 8 cut(s) 204, 216, 222, 374, 436, 524, 544, 737
HpaII CCGG 2 cut(s) 141, 484
HphI GGTGA 4 cut(s) 69, 293, 564, 633
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 7 cut(s) 15, 221, 227, 341, 681, 736, 952
Hpy188III TCNNGA 8 cut(s) 84, 197, 318, 327, 548, 609, 655, 758
Hpy8I GTNNAC 1 cut(s) 727
Hpy99I CGWCG 1 cut(s) 276
HpyAV CCTTC 5 cut(s) 450, 546, 832, 929, 994
HpyCH4III ACNGT 6 cut(s) 118, 291, 297, 506, 639, 968
HpyCH4V TGCA 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 276, 329, 802
HpyF3I CTNAG 4 cut(s) 333, 747, 870, 900
Hsp92II CATG 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 697
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 1 cut(s) 253
MaeI CTAG 7 cut(s) 197, 261, 267, 327, 498, 548, 828
MaeIII GTNAC 2 cut(s) 291, 639
MflI RGATCY 4 cut(s) 227, 256, 336, 810
MluCI AATT 1 cut(s) 1005
MlyI GAGTC 1 cut(s) 213
MnlI CCTC 9 cut(s) 44, 48, 140, 142, 199, 334, 462, 801, 888
MseI TTAA 3 cut(s) 153, 1008, 1018
MspI CCGG 2 cut(s) 141, 484
MspR9I CCNGG 3 cut(s) 541, 889, 936
MvaI CCWGG 3 cut(s) 541, 889, 936
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 4 cut(s) 165, 276, 329, 802
NlaIII CATG 1 cut(s) 168
NmeAIII GCCGAG 1 cut(s) 831
NmuCI GTSAC 1 cut(s) 639
PfeI GAWTC 7 cut(s) 216, 222, 374, 436, 524, 544, 737
PflFI GACNNNGTC 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 2 cut(s) 243, 278
Ple19I CGATCG 1 cut(s) 317
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 3 cut(s) 539, 887, 934
PspGI CCWGG 3 cut(s) 539, 887, 934
PspPI GGNCC 1 cut(s) 481
PsuI RGATCY 4 cut(s) 227, 256, 336, 810
PsyI GACNNNGTC 1 cut(s) 203
PvuI CGATCG 1 cut(s) 317
RsaI GTAC 2 cut(s) 288, 490
RsaNI GTAC 2 cut(s) 287, 489
SaqAI TTAA 3 cut(s) 153, 1008, 1018
SatI GCNGC 2 cut(s) 242, 277
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 3 cut(s) 541, 889, 936
Sse9I AATT 1 cut(s) 1005
SsiI CCGC 6 cut(s) 159, 276, 279, 366, 419, 467
SspMI CTAG 7 cut(s) 197, 261, 267, 327, 498, 548, 828
StyD4I CCNGG 3 cut(s) 539, 887, 934
TaaI ACNGT 6 cut(s) 118, 291, 297, 506, 639, 968
TaqI TCGA 4 cut(s) 317, 431, 556, 654
TasI AATT 1 cut(s) 1005
TatI WGTACW 2 cut(s) 286, 488
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 7 cut(s) 216, 222, 374, 436, 524, 544, 737
Tru1I TTAA 3 cut(s) 153, 1008, 1018
Tru9I TTAA 3 cut(s) 153, 1008, 1018
TscAI CASTG 1 cut(s) 302
TseFI GTSAC 1 cut(s) 639
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 639
TspDTI ATGAA 3 cut(s) 449, 875, 987
TspGWI ACGGA 2 cut(s) 216, 582
TspRI CASTG 1 cut(s) 302
Tth111I GACNNNGTC 1 cut(s) 203
XbaI TCTAGA 3 cut(s) 196, 326, 547
XspI CTAG 7 cut(s) 197, 261, 267, 327, 498, 548, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.