MD05G1241700.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006362
Physical Location & Seq
Reverse (-)
37486832 .. 37491253
4422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1241700.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGATGATGAGGTACCAGAGAGTGAGCCCAGATTGCGTCCCTCTGAGCAATGGCAAGAAACCAACCATGAGAGCCATATCCAAAGACGACGGGATTACAGACGCCGTGACAACAAACACTACAGTCACATCTCTAGAACCCACCAAACCCTTCAGATTCCGATCCCAACCCACGACTCAAGACCCGACCCAATCCCAATTCGGAGCCCGACCCACTTCCCCAAACGGTGAAATCCACCACCAGACCCAGCAGCGGCAGGACAAGAGTCCCAGCCGGACTCCCAGTCCAATCCGCGGCGCCGGCGATGTGCTGCTGCAGTGGGGCCAGAGAAAGAGGTCGAGAGTCTCCAGGACCGAGATCCGAGCTGTCACCGATGAGTCCTCTTCGTCGGCTCAAGCGAGGCAAGCTTCAAAGCTGCAGAGGAGAGACAAGTCCATGCCCCCGCCTCCTCCCCCGCCGCCCCTTCCGTCTTCTTGCTCCGCTACGTCGTCGTTTTCCAATGGCAGCAGACTGAGAAAGGAAGCCTCCGGGTCGCTTCCTAGCAGGAATTTGGTAGATCGATCTGCTCCCGTTAATGGGTCACCATCGAGAAACCCTACTGGTGGCAGCAACGGCCGAGCTGCTTCCAGATCCACGGCGGGGAAACGATCTCCTCCGCCGGAGAAAAATGAGAGAAAGGTGCCGGCTTGCTCAGGCAGGTCATCAGCAGCAGCCAAAGATGACAACAAGCCAAATGGAGCTCAAGCTGATCGCGGGAATCATGCTGATTCCAGTTTGTTGCAATCGGATCAGTTGGCCGTCGGGGCAGGGGCGGATAATTCTTCCGCCGCAGCTGCAACGGCGGCAGCGGAGAAGGTGAACTACGAAGTGGTGGAGTGGCCGAGAATTTATATTGCTCTGTCGAGGAAGGAGAAGGAAGATGATTTCCTTGCAATGAAAGGCACAAAGCTCCCCCAGAGGCCCAAAAAAAGGGCCAAAAATGTTGACAGAACTCTACAGTATTGTTTTCCAGGTATGTGGCTGTCTGACTTAACAAGGAATCGATATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGCAAAAGCGAAGGGGACTCAAAGGAATGGACAGCGTCGACAGTGATTCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

377

Amino Acids

41.01

Weight (kDa)

10.27

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 300 - 349 8.2e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 3 cut(s) 12, 296, 679
AccI GTMKAC 1 cut(s) 1113
AccII CGCG 2 cut(s) 294, 753
AclWI GGATC 4 cut(s) 156, 352, 624, 795
AcoI YGGCCR 3 cut(s) 613, 795, 878
AcsI RAATTY 2 cut(s) 547, 885
AcuI CTGAAG 1 cut(s) 136
AcyI GRCGYC 2 cut(s) 102, 297
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 7 cut(s) 252, 293, 575, 576, 602, 639, 969
AgsI TTSAA 1 cut(s) 411
AhdI GACNNNNNGTC 1 cut(s) 282
AjnI CCWGG 2 cut(s) 347, 1009
AluBI AGCT 8 cut(s) 365, 407, 415, 620, 740, 746, 833, 949
AluI AGCT 8 cut(s) 365, 407, 415, 620, 740, 746, 833, 949
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 2 cut(s) 349, 420
AlwI GGATC 4 cut(s) 156, 352, 624, 795
AoxI GGCC 6 cut(s) 322, 613, 795, 878, 959, 972
ApoI RAATTY 2 cut(s) 547, 885
Asp718I GGTACC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 4 cut(s) 322, 351, 960, 972
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 4 cut(s) 239, 361, 573, 868
AvaII GGWCC 1 cut(s) 351
BanI GGYRCC 3 cut(s) 12, 296, 679
BanII GRGCYC 3 cut(s) 29, 208, 742
BarI GAAGNNNNNNTAC 2 cut(s) 845, 877
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 742
BccI CCATC 1 cut(s) 592
BceAI ACGGC 5 cut(s) 89, 628, 651, 782, 855
BciT130I CCWGG 2 cut(s) 349, 1011
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 349, 420
BfaI CTAG 2 cut(s) 134, 540
BfmI CTRYAG 4 cut(s) 120, 314, 416, 995
BfoI RGCGCY 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 687
BglI GCCNNNNNGGC 1 cut(s) 803
Bme1390I CCNGG 3 cut(s) 349, 529, 1011
Bme18I GGWCC 1 cut(s) 351
BmeRI GACNNNNNGTC 1 cut(s) 282
BmgT120I GGNCC 4 cut(s) 322, 351, 960, 972
BmiI GGNNCC 5 cut(s) 14, 205, 298, 323, 681
BmrFI CCNGG 3 cut(s) 349, 529, 1011
BmrI ACTGGG 1 cut(s) 276
BmuI ACTGGG 1 cut(s) 276
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 691
BpuEI CTTGAG 3 cut(s) 162, 378, 726
BpuMI CCSGG 1 cut(s) 529
Bsa29I ATCGAT 2 cut(s) 559, 1042
BsaBI GATNNNNATC 1 cut(s) 160
BsaHI GRCGYC 2 cut(s) 102, 297
BsaJI CCNNGG 2 cut(s) 292, 633
Bsc4I CCNNNNNNNGG 7 cut(s) 252, 293, 575, 576, 602, 639, 969
Bse118I RCCGGY 2 cut(s) 299, 682
Bse1I ACTGG 3 cut(s) 282, 604, 771
Bse3DI GCAATG 2 cut(s) 55, 939
Bse8I GATNNNNATC 1 cut(s) 160
BseBI CCWGG 2 cut(s) 349, 1011
BseCI ATCGAT 2 cut(s) 559, 1042
BseDI CCNNGG 2 cut(s) 292, 633
BseJI GATNNNNATC 1 cut(s) 160
BseLI CCNNNNNNNGG 7 cut(s) 252, 293, 575, 576, 602, 639, 969
BseMI GCAATG 2 cut(s) 55, 939
BseMII CTCAG 3 cut(s) 35, 503, 705
BseNI ACTGG 3 cut(s) 282, 604, 771
BseRI GAGGAG 3 cut(s) 436, 438, 642
BseX3I CGGCCG 1 cut(s) 613
BseYI CCCAGC 2 cut(s) 246, 269
Bsh1236I CGCG 2 cut(s) 294, 753
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 6 cut(s) 324, 615, 797, 880, 961, 974
BshNI GGYRCC 3 cut(s) 12, 296, 679
BshVI ATCGAT 2 cut(s) 559, 1042
BsiEI CGRYCG 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 5 cut(s) 274, 300, 528, 659, 683
BslFI GGGAC 3 cut(s) 23, 252, 1104
BslI CCNNNNNNNGG 7 cut(s) 252, 293, 575, 576, 602, 639, 969
BsmAI GTCTC 2 cut(s) 349, 420
BsmFI GGGAC 3 cut(s) 23, 252, 1104
BsnI GGCC 6 cut(s) 324, 615, 797, 880, 961, 974
Bsp1286I GDGCHC 3 cut(s) 29, 208, 742
Bsp143I GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
BspANI GGCC 6 cut(s) 324, 615, 797, 880, 961, 974
BspCNI CTCAG 3 cut(s) 36, 504, 704
BspDI ATCGAT 2 cut(s) 559, 1042
BspFNI CGCG 2 cut(s) 294, 753
BspLI GGNNCC 5 cut(s) 14, 205, 298, 323, 681
BspMAI CTGCAG 2 cut(s) 318, 420
BspMI ACCTGC 1 cut(s) 687
BspPI GGATC 4 cut(s) 156, 352, 624, 795
BspT107I GGYRCC 3 cut(s) 12, 296, 679
BsrDI GCAATG 2 cut(s) 55, 939
BsrFI RCCGGY 2 cut(s) 299, 682
BsrI ACTGG 3 cut(s) 282, 604, 771
BssAI RCCGGY 2 cut(s) 299, 682
BssECI CCNNGG 2 cut(s) 292, 633
BssMI GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
BssNI GRCGYC 2 cut(s) 102, 297
Bst2UI CCWGG 2 cut(s) 349, 1011
Bst4CI ACNGT 4 cut(s) 124, 227, 999, 1118
Bst6I CTCTTC 1 cut(s) 388
BstACI GRCGYC 2 cut(s) 102, 297
BstC8I GCNNGC 4 cut(s) 301, 405, 684, 688
BstDEI CTNAG 3 cut(s) 44, 512, 691
BstDSI CCRYGG 2 cut(s) 292, 633
BstEII GGTNACC 1 cut(s) 579
BstFNI CGCG 2 cut(s) 294, 753
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 8 cut(s) 164, 360, 559, 563, 632, 650, 751, 790
BstMAI GTCTC 2 cut(s) 349, 420
BstMBI GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
BstMCI CGRYCG 1 cut(s) 616
BstMWI GCNNNNNNNGC 8 cut(s) 33, 300, 404, 612, 803, 833, 839, 842
BstNI CCWGG 2 cut(s) 349, 1011
BstPAI GACNNNNGTC 1 cut(s) 264
BstPI GGTNACC 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 347, 527, 1009
BstSFI CTRYAG 4 cut(s) 120, 314, 416, 995
BstUI CGCG 2 cut(s) 294, 753
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 2 cut(s) 357, 629
BstXI CCANNNNNNTGG 1 cut(s) 1017
BstYI RGATCY 2 cut(s) 357, 629
BstZI CGGCCG 1 cut(s) 613
Bsu15I ATCGAT 2 cut(s) 559, 1042
BsuRI GGCC 6 cut(s) 324, 615, 797, 880, 961, 974
BsuTUI ATCGAT 2 cut(s) 559, 1042
BtgI CCRYGG 2 cut(s) 292, 633
BtgZI GCGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 323, 1123
BveI ACCTGC 1 cut(s) 687
Cac8I GCNNGC 4 cut(s) 301, 405, 684, 688
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 2 cut(s) 299, 682
Cfr13I GGNCC 4 cut(s) 322, 351, 960, 972
Cfr42I CCGCGG 1 cut(s) 295
ClaI ATCGAT 2 cut(s) 559, 1042
CseI GACGC 3 cut(s) 25, 110, 1099
Csp6I GTAC 1 cut(s) 13
CviAII CATG 3 cut(s) 67, 436, 761
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 3 cut(s) 44, 512, 691
DinI GGCGCC 1 cut(s) 298
DpnI GATC 8 cut(s) 163, 359, 558, 562, 631, 649, 750, 789
DpnII GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
DriI GACNNNNNGTC 1 cut(s) 282
EaeI YGGCCR 3 cut(s) 613, 795, 878
EagI CGGCCG 1 cut(s) 613
Eam1104I CTCTTC 1 cut(s) 388
Eam1105I GACNNNNNGTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 388
EciI GGCGGA 3 cut(s) 645, 814, 827
Ecl136II GAGCTC 1 cut(s) 740
EclXI CGGCCG 1 cut(s) 613
Eco24I GRGCYC 3 cut(s) 29, 208, 742
Eco47I GGWCC 1 cut(s) 351
Eco52I CGGCCG 1 cut(s) 613
Eco53kI GAGCTC 1 cut(s) 740
Eco57I CTGAAG 1 cut(s) 136
Eco91I GGTNACC 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 740
EcoO65I GGTNACC 1 cut(s) 579
EcoRII CCWGG 2 cut(s) 347, 1009
EcoT38I GRGCYC 3 cut(s) 29, 208, 742
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaeI CATG 3 cut(s) 70, 439, 764
FaiI YATR 7 cut(s) 68, 77, 437, 762, 891, 1016, 1047
FaqI GGGAC 3 cut(s) 23, 252, 1104
FatI CATG 3 cut(s) 66, 435, 760
FauI CCCGC 4 cut(s) 450, 462, 631, 746
FblI GTMKAC 1 cut(s) 1113
FriOI GRGCYC 3 cut(s) 29, 208, 742
FspBI CTAG 2 cut(s) 134, 540
GlaI GCGC 1 cut(s) 298
GsaI CCCAGC 2 cut(s) 250, 273
GsuI CTGGAG 1 cut(s) 331
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 6 cut(s) 324, 615, 797, 880, 961, 974
HapII CCGG 5 cut(s) 274, 300, 528, 659, 683
HgaI GACGC 3 cut(s) 25, 110, 1099
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 2 cut(s) 102, 297
Hin1II CATG 3 cut(s) 70, 439, 764
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HincII GTYRAC 2 cut(s) 985, 1114
HindII GTYRAC 2 cut(s) 985, 1114
HindIII AAGCTT 1 cut(s) 405
HpaII CCGG 5 cut(s) 274, 300, 528, 659, 683
HphI GGTGA 4 cut(s) 239, 361, 573, 868
Hpy166II GTNNAC 3 cut(s) 859, 985, 1114
Hpy188I TCNGA 8 cut(s) 45, 155, 161, 203, 362, 787, 1027, 1126
Hpy188III TCNNGA 5 cut(s) 134, 179, 339, 588, 627
Hpy8I GTNNAC 3 cut(s) 859, 985, 1114
Hpy99I CGWCG 6 cut(s) 92, 391, 490, 493, 803, 1115
HpyAV CCTTC 6 cut(s) 160, 473, 847, 901, 907, 1080
HpyCH4III ACNGT 4 cut(s) 124, 227, 999, 1118
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 5 cut(s) 316, 418, 781, 836, 932
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 300, 404, 612, 803, 833, 839, 842
HpyF3I CTNAG 3 cut(s) 44, 512, 691
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 2 cut(s) 102, 297
Hsp92II CATG 3 cut(s) 70, 439, 764
HspAI GCGC 1 cut(s) 297
KasI GGCGCC 1 cut(s) 296
KpnI GGTACC 1 cut(s) 16
KroI GCCGGC 2 cut(s) 299, 682
KroNI GCCGGC 2 cut(s) 301, 684
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
LmnI GCTCC 5 cut(s) 203, 482, 571, 737, 954
MaeI CTAG 2 cut(s) 134, 540
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 4 cut(s) 106, 124, 367, 579
MalI GATC 8 cut(s) 163, 359, 558, 562, 631, 649, 750, 789
MboI GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
MboII GAAGA 6 cut(s) 375, 462, 813, 929, 1072, 1084
MflI RGATCY 2 cut(s) 357, 629
MhlI GDGCHC 3 cut(s) 29, 208, 742
MluCI AATT 4 cut(s) 197, 547, 817, 885
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 6 cut(s) 169, 271, 274, 351, 386, 1086
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 2 cut(s) 299, 682
MseI TTAA 2 cut(s) 573, 1031
MspA1I CMGCKG 4 cut(s) 253, 294, 833, 848
MspI CCGG 5 cut(s) 274, 300, 528, 659, 683
MspR9I CCNGG 3 cut(s) 349, 529, 1011
MvaI CCWGG 2 cut(s) 349, 1011
MvnI CGCG 2 cut(s) 294, 753
MwoI GCNNNNNNNGC 8 cut(s) 33, 300, 404, 612, 803, 833, 839, 842
NaeI GCCGGC 2 cut(s) 301, 684
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 529
NdeII GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
NgoMIV GCCGGC 2 cut(s) 299, 682
NlaIII CATG 3 cut(s) 70, 439, 764
NlaIV GGNNCC 5 cut(s) 14, 205, 298, 323, 681
NmeAIII GCCGAG 2 cut(s) 641, 906
NmuCI GTSAC 4 cut(s) 106, 124, 367, 579
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PdiI GCCGGC 2 cut(s) 301, 684
PfeI GAWTC 5 cut(s) 156, 757, 767, 1039, 1121
PflFI GACNNNGTC 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 347
PleI GAGTC 6 cut(s) 169, 271, 273, 350, 385, 1086
PluTI GGCGCC 1 cut(s) 300
PpsI GAGTC 6 cut(s) 169, 271, 273, 350, 385, 1086
PshAI GACNNNNGTC 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 742
Psp6I CCWGG 2 cut(s) 347, 1009
PspEI GGTNACC 1 cut(s) 579
PspFI CCCAGC 2 cut(s) 246, 269
PspGI CCWGG 2 cut(s) 347, 1009
PspN4I GGNNCC 5 cut(s) 14, 205, 298, 323, 681
PspPI GGNCC 4 cut(s) 322, 351, 960, 972
PstI CTGCAG 2 cut(s) 318, 420
PsuI RGATCY 2 cut(s) 357, 629
PsyI GACNNNGTC 1 cut(s) 1109
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SacI GAGCTC 1 cut(s) 742
SacII CCGCGG 1 cut(s) 295
SalI GTCGAC 1 cut(s) 1112
SaqAI TTAA 2 cut(s) 573, 1031
Sau3AI GATC 8 cut(s) 161, 357, 556, 560, 629, 647, 748, 787
Sau96I GGNCC 4 cut(s) 322, 351, 960, 972
SchI GAGTC 6 cut(s) 169, 271, 274, 351, 386, 1086
ScrFI CCNGG 3 cut(s) 349, 529, 1011
SduI GDGCHC 3 cut(s) 29, 208, 742
SfcI CTRYAG 4 cut(s) 120, 314, 416, 995
SfoI GGCGCC 1 cut(s) 298
Sfr303I CCGCGG 1 cut(s) 295
SgrAI CRCCGGYG 1 cut(s) 299
SgrBI CCGCGG 1 cut(s) 295
SinI GGWCC 1 cut(s) 351
SmlI CTYRAG 3 cut(s) 177, 393, 741
SmoI CTYRAG 3 cut(s) 177, 393, 741
Sse9I AATT 4 cut(s) 197, 547, 817, 885
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 2 cut(s) 134, 540
SstI GAGCTC 1 cut(s) 742
StyD4I CCNGG 3 cut(s) 347, 527, 1009
TaaI ACNGT 4 cut(s) 124, 227, 999, 1118
TaiI ACGT 1 cut(s) 488
TaqI TCGA 6 cut(s) 338, 559, 587, 902, 1042, 1113
TaqII GACCGA 1 cut(s) 368
TasI AATT 4 cut(s) 197, 547, 817, 885
TauI GCSGC 5 cut(s) 256, 297, 460, 830, 845
TfiI GAWTC 5 cut(s) 156, 757, 767, 1039, 1121
Tru1I TTAA 2 cut(s) 573, 1031
Tru9I TTAA 2 cut(s) 573, 1031
TscAI CASTG 2 cut(s) 323, 1123
TseFI GTSAC 4 cut(s) 106, 124, 367, 579
Tsp45I GTSAC 4 cut(s) 106, 124, 367, 579
TspDTI ATGAA 1 cut(s) 950
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 323, 1123
Tth111I GACNNNGTC 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 2 cut(s) 547, 885
XbaI TCTAGA 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 1113
XspI CTAG 2 cut(s) 134, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.