FvH4_3g13430

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006362
Physical Location & Seq
Forward (+)
8036051 .. 8040655
4605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13430.t3

Sequence Viewer

Length: 1017 bp
ATGATGACAGAGAACAGCACCAATACAACAACCAGCACAGTCTCTTCACTGGAATCCAAACCCTTCAGATTCAGAGACCCAAGTCCCCTCTCCCAATTCGGAGGCGTACCACCCACTTCTCCAAGCCCCGAAAACCGCCCCACTCACAGCAGCAGCCCCGGCCGCACTCCCAGCCCCAGCCGCGCCGGCTCCGGTGGCGACGTGCTGCTCCAGTGGGGCCAGAGAAAGAGGTCCCGGGTCTCGAGAACCGAGATCCGGGTCTTGGCTGATGAGTCCTCCTCATCAGCCCAAGCAAGACAAGCCAAAGTGCAAAGGAGAGCGGCTCACGCTGCAGCCGTGGCAGCTGACAAGTCCATGCCTCCGCCGCCTCCTCCTCCGCCACCGCACCCATCTTCCACCACCTCAACGTCGTCGTTTTCCAATGGTAGACTCCGGAAGGAAGCCTCAGGGTTGCTTCCAAACAGGAATTTGGAAGATCGATCCGCTGTGGTTAATGGGTCACCCTCTAGAAGCACTGTGGTCGGGAACGGCCGTGCTGCTTCTAGATCTATAGCGGGCAAAAGGTCTCCTCCCCCAGAGAAAAGTGAGAGAAAGATGCCTTCTTGTTCTGGAAGGTCAGCAAAAGATGACAAGGCAAATGGGTCATCTGATCACCGGGCAAACCATGTCGATTCGACCTCATTACAATCGGAGCAATTAGCTGGAGCTGCTAACCATTCTGCTGCACTAGCAGCTGAAAAACTGAACCATGAAGTGGTCGAGTGGCCAAGAATTTACTTGGCTTTGTCAAGGAAGGAGAAAGAAGATGATTTCCTTGCAATGAAAGGCACGAAGCTGCCTCAGAGACCCAAGAAGAGGGCCAAGAACGTTGACAGAACCCTTCAGTATTGTTTCCCAGGGATGTGGCTATCTGACTTGACAAGGAATCGCTATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGCAGAAGCGAAGGGGACTGAAAGGGATGGAGAGCGTGGAAAGCGAATCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

339

Amino Acids

36.78

Weight (kDa)

10.4

Isoelectric Point (pI)

69.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 307 - 356 8.4e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 427
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 432
AclI AACGTT 1 cut(s) 867
AclWI GGATC 2 cut(s) 247, 474
AcoI YGGCCR 3 cut(s) 160, 529, 764
AcsI RAATTY 2 cut(s) 466, 771
AcuI CTGAAG 2 cut(s) 49, 866
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 4 cut(s) 255, 262, 754, 855
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 1 cut(s) 895
AluBI AGCT 5 cut(s) 344, 701, 707, 734, 835
AluI AGCT 5 cut(s) 344, 701, 707, 734, 835
Alw26I GTCTC 5 cut(s) 46, 69, 244, 570, 838
AlwI GGATC 2 cut(s) 247, 474
Ama87I CYCGRG 2 cut(s) 234, 241
Aor13HI TCCGGA 1 cut(s) 432
AoxI GGCC 5 cut(s) 160, 217, 529, 764, 858
ApoI RAATTY 2 cut(s) 466, 771
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 217, 231, 858
AsuC2I CCSGG 5 cut(s) 159, 235, 236, 257, 656
AsuHPI GGTGA 2 cut(s) 492, 644
AvaI CYCGRG 2 cut(s) 234, 241
AvaII GGWCC 1 cut(s) 231
AxyI CCTNAGG 1 cut(s) 445
BalI TGGCCA 1 cut(s) 766
BccI CCATC 2 cut(s) 397, 982
BceAI ACGGC 3 cut(s) 320, 516, 544
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 1 cut(s) 897
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 5 cut(s) 159, 235, 236, 257, 656
BcoDI GTCTC 5 cut(s) 46, 69, 244, 570, 838
BfaI CTAG 3 cut(s) 507, 543, 728
BfmI CTRYAG 2 cut(s) 330, 549
BglI GCCNNNNNGGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 545
Bme1390I CCNGG 6 cut(s) 159, 235, 236, 257, 656, 897
Bme18I GGWCC 1 cut(s) 231
BmeT110I CYCGRG 2 cut(s) 234, 241
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 3 cut(s) 217, 231, 858
BmiI GGNNCC 3 cut(s) 190, 218, 233
BmrFI CCNGG 6 cut(s) 159, 235, 236, 257, 656, 897
BmsI GCATC 1 cut(s) 585
BoxI GACNNNNGTC 1 cut(s) 81
BplI GAGNNNNNCTC 4 cut(s) 263, 295, 307, 339
BpmI CTGGAG 2 cut(s) 194, 723
BpuMI CCSGG 5 cut(s) 159, 235, 236, 257, 656
Bsa29I ATCGAT 1 cut(s) 478
BsaI GGTCTC 4 cut(s) 69, 244, 570, 838
BsaJI CCNNGG 5 cut(s) 157, 234, 336, 895, 896
BsaWI WCCGGW 2 cut(s) 191, 432
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 262, 754, 855
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 54, 211
Bse21I CCTNAGG 1 cut(s) 445
Bse3DI GCAATG 1 cut(s) 825
BseAI TCCGGA 1 cut(s) 432
BseBI CCWGG 1 cut(s) 897
BseCI ATCGAT 1 cut(s) 478
BseDI CCNNGG 5 cut(s) 157, 234, 336, 895, 896
BseGI GGATG 2 cut(s) 906, 993
BseLI CCNNNNNNNGG 4 cut(s) 255, 262, 754, 855
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 2 cut(s) 459, 854
BseNI ACTGG 2 cut(s) 54, 211
BseRI GAGGAG 4 cut(s) 268, 360, 363, 558
BseX3I CGGCCG 2 cut(s) 160, 529
BseYI CCCAGC 2 cut(s) 170, 176
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 2 cut(s) 163, 532
BshFI GGCC 5 cut(s) 162, 219, 531, 766, 860
BshVI ATCGAT 1 cut(s) 478
BsiEI CGRYCG 2 cut(s) 163, 532
BsiHKCI CYCGRG 2 cut(s) 234, 241
BsiSI CCGG 7 cut(s) 159, 186, 192, 235, 256, 433, 655
BslFI GGGAC 3 cut(s) 69, 217, 990
BslI CCNNNNNNNGG 4 cut(s) 255, 262, 754, 855
BsmAI GTCTC 5 cut(s) 46, 69, 244, 570, 838
BsmFI GGGAC 3 cut(s) 69, 217, 990
BsnI GGCC 5 cut(s) 162, 219, 531, 766, 860
Bso31I GGTCTC 4 cut(s) 69, 244, 570, 838
BsoBI CYCGRG 2 cut(s) 234, 241
Bsp13I TCCGGA 1 cut(s) 432
Bsp143I GATC 5 cut(s) 252, 475, 479, 545, 649
BspANI GGCC 5 cut(s) 162, 219, 531, 766, 860
BspCNI CTCAG 2 cut(s) 458, 853
BspDI ATCGAT 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 432
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 190, 218, 233
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 2 cut(s) 247, 474
BspTNI GGTCTC 4 cut(s) 69, 244, 570, 838
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 54, 211
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 5 cut(s) 157, 234, 336, 895, 896
BssMI GATC 5 cut(s) 252, 475, 479, 545, 649
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 2 cut(s) 40, 517
Bst6I CTCTTC 2 cut(s) 49, 848
BstC8I GCNNGC 2 cut(s) 187, 556
BstDEI CTNAG 2 cut(s) 445, 840
BstDSI CCRYGG 1 cut(s) 336
BstEII GGTNACC 1 cut(s) 498
BstF5I GGATG 2 cut(s) 906, 993
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 5 cut(s) 255, 478, 482, 548, 652
BstMAI GTCTC 5 cut(s) 46, 69, 244, 570, 838
BstMBI GATC 5 cut(s) 252, 475, 479, 545, 649
BstMCI CGRYCG 2 cut(s) 163, 532
BstNI CCWGG 1 cut(s) 897
BstPAI GACNNNNGTC 1 cut(s) 81
BstPI GGTNACC 1 cut(s) 498
BstSCI CCNGG 6 cut(s) 157, 233, 234, 255, 654, 895
BstSFI CTRYAG 2 cut(s) 330, 549
BstUI CGCG 1 cut(s) 183
BstX2I RGATCY 2 cut(s) 252, 545
BstXI CCANNNNNNTGG 1 cut(s) 903
BstYI RGATCY 2 cut(s) 252, 545
BstZI CGGCCG 2 cut(s) 160, 529
Bsu15I ATCGAT 1 cut(s) 478
Bsu36I CCTNAGG 1 cut(s) 445
BsuRI GGCC 5 cut(s) 162, 219, 531, 766, 860
BsuTUI ATCGAT 1 cut(s) 478
BtgI CCRYGG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 2 cut(s) 906, 993
BtsIMutI CAGTG 3 cut(s) 47, 218, 513
Cac8I GCNNGC 2 cut(s) 187, 556
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 217, 231, 858
Cfr9I CCCGGG 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 478
Csp6I GTAC 1 cut(s) 107
CviAII CATG 3 cut(s) 355, 665, 749
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 445, 840
DpnI GATC 5 cut(s) 254, 477, 481, 547, 651
DpnII GATC 5 cut(s) 252, 475, 479, 545, 649
EaeI YGGCCR 3 cut(s) 160, 529, 764
EagI CGGCCG 2 cut(s) 160, 529
Eam1104I CTCTTC 2 cut(s) 49, 848
EarI CTCTTC 2 cut(s) 49, 848
EciI GGCGGA 2 cut(s) 351, 366
EclXI CGGCCG 2 cut(s) 160, 529
Eco31I GGTCTC 4 cut(s) 69, 244, 570, 838
Eco47I GGWCC 1 cut(s) 231
Eco52I CGGCCG 2 cut(s) 160, 529
Eco57I CTGAAG 2 cut(s) 49, 866
Eco81I CCTNAGG 1 cut(s) 445
Eco88I CYCGRG 2 cut(s) 234, 241
Eco91I GGTNACC 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 231
EcoO65I GGTNACC 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 895
FaeI CATG 3 cut(s) 358, 668, 752
FaiI YATR 5 cut(s) 356, 551, 666, 750, 933
FaqI GGGAC 3 cut(s) 69, 217, 990
FatI CATG 3 cut(s) 354, 664, 748
FauI CCCGC 1 cut(s) 547
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 1 cut(s) 427
FokI GGATG 2 cut(s) 913, 1000
FspBI CTAG 3 cut(s) 507, 543, 728
GlaI GCGC 1 cut(s) 184
GsaI CCCAGC 2 cut(s) 174, 180
GsuI CTGGAG 2 cut(s) 194, 723
HaeIII GGCC 5 cut(s) 162, 219, 531, 766, 860
HapII CCGG 7 cut(s) 159, 186, 192, 235, 256, 433, 655
HhaI GCGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 358, 668, 752
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 7 cut(s) 53, 69, 272, 429, 671, 925, 1007
HpaII CCGG 7 cut(s) 159, 186, 192, 235, 256, 433, 655
HphI GGTGA 2 cut(s) 492, 644
Hpy166II GTNNAC 2 cut(s) 428, 871
Hpy188I TCNGA 8 cut(s) 68, 74, 101, 649, 691, 843, 913, 1012
Hpy188III TCNNGA 7 cut(s) 241, 243, 433, 507, 523, 543, 609
Hpy8I GTNNAC 2 cut(s) 428, 871
Hpy99I CGWCG 3 cut(s) 203, 412, 415
HpyAV CCTTC 7 cut(s) 73, 430, 606, 609, 787, 890, 966
HpyCH4III ACNGT 2 cut(s) 40, 517
HpyCH4IV ACGT 3 cut(s) 201, 407, 867
HpyCH4V TGCA 4 cut(s) 310, 332, 725, 818
HpyF3I CTNAG 2 cut(s) 445, 840
HpySE526I ACGT 3 cut(s) 201, 407, 867
Hsp92II CATG 3 cut(s) 358, 668, 752
HspAI GCGC 1 cut(s) 183
Kpn2I TCCGGA 1 cut(s) 432
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 5 cut(s) 252, 475, 479, 545, 649
LmnI GCTCC 4 cut(s) 194, 213, 691, 704
LweI GCATC 1 cut(s) 585
MaeI CTAG 3 cut(s) 507, 543, 728
MaeII ACGT 3 cut(s) 201, 407, 867
MaeIII GTNAC 1 cut(s) 498
MalI GATC 5 cut(s) 254, 477, 481, 547, 651
MbiI CCGCTC 1 cut(s) 320
MboI GATC 5 cut(s) 252, 475, 479, 545, 649
MboII GAAGA 7 cut(s) 36, 384, 485, 815, 865, 958, 970
MflI RGATCY 2 cut(s) 252, 545
MlsI TGGCCA 1 cut(s) 766
MluCI AATT 4 cut(s) 95, 466, 695, 771
MluNI TGGCCA 1 cut(s) 766
MlyI GAGTC 2 cut(s) 281, 423
Mox20I TGGCCA 1 cut(s) 766
MroI TCCGGA 1 cut(s) 432
MroNI GCCGGC 1 cut(s) 185
MscI TGGCCA 1 cut(s) 766
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 766
MspA1I CMGCKG 3 cut(s) 344, 485, 734
MspI CCGG 7 cut(s) 159, 186, 192, 235, 256, 433, 655
MspR9I CCNGG 6 cut(s) 159, 235, 236, 257, 656, 897
MvaI CCWGG 1 cut(s) 897
MvnI CGCG 1 cut(s) 183
NaeI GCCGGC 1 cut(s) 187
NciI CCSGG 5 cut(s) 159, 235, 236, 257, 656
NdeII GATC 5 cut(s) 252, 475, 479, 545, 649
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 3 cut(s) 358, 668, 752
NlaIV GGNNCC 3 cut(s) 190, 218, 233
NmuCI GTSAC 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 241
PasI CCCWGGG 1 cut(s) 896
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 5 cut(s) 53, 69, 671, 925, 1007
PflMI CCANNNNNTGG 1 cut(s) 754
PleI GAGTC 2 cut(s) 280, 423
PpsI GAGTC 2 cut(s) 280, 423
PpuMI RGGWCCY 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 81
Psp1406I AACGTT 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 895
PspEI GGTNACC 1 cut(s) 498
PspFI CCCAGC 2 cut(s) 170, 176
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 3 cut(s) 190, 218, 233
PspPI GGNCC 3 cut(s) 217, 231, 858
PspPPI RGGWCCY 1 cut(s) 231
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 2 cut(s) 252, 545
PvuII CAGCTG 2 cut(s) 344, 734
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 5 cut(s) 252, 475, 479, 545, 649
Sau96I GGNCC 3 cut(s) 217, 231, 858
SchI GAGTC 2 cut(s) 281, 423
ScrFI CCNGG 6 cut(s) 159, 235, 236, 257, 656, 897
SfaNI GCATC 1 cut(s) 585
SfcI CTRYAG 2 cut(s) 330, 549
Sfr274I CTCGAG 1 cut(s) 241
SinI GGWCC 1 cut(s) 231
SlaI CTCGAG 1 cut(s) 241
SmaI CCCGGG 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 4 cut(s) 95, 466, 695, 771
SspMI CTAG 3 cut(s) 507, 543, 728
StyD4I CCNGG 6 cut(s) 157, 233, 234, 255, 654, 895
TaaI ACNGT 2 cut(s) 40, 517
TaiI ACGT 3 cut(s) 204, 410, 870
TaqI TCGA 5 cut(s) 242, 478, 669, 674, 759
TasI AATT 4 cut(s) 95, 466, 695, 771
TauI GCSGC 4 cut(s) 165, 183, 323, 367
TfiI GAWTC 5 cut(s) 53, 69, 671, 925, 1007
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 3 cut(s) 54, 218, 520
TseFI GTSAC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 2 cut(s) 765, 836
TspMI CCCGGG 1 cut(s) 234
TspRI CASTG 3 cut(s) 54, 218, 520
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 1 cut(s) 231
XapI RAATTY 2 cut(s) 466, 771
XbaI TCTAGA 2 cut(s) 506, 542
XhoI CTCGAG 1 cut(s) 241
XmaI CCCGGG 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 427
XspI CTAG 3 cut(s) 507, 543, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.