Rh5BG159300

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006362
Physical Location & Seq
Forward (+)
16261755 .. 16266024
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG159300.1

Sequence Viewer

Length: 1188 bp
ATGACATTTGACTTGACTCTCCGAGTTCATTTTCTTAATCTGCATTCGATTTTGTTAATCCGGTCGTTATTGAATTGTGTTCGCAGGTACCAGAGAGTAAGCCCAGATTGTGTTCCTCTCAGCAATGGCAAGAAACCCAGCATAAGAGCACTGCCAAAAGAAGACATGATGACAGAGAACACAACCACCGCAACAAGCAGCACAGTTTCTTCGTTGGAATCCAAACCCTTCAGATTCCGATCCCAACCCACAGCCCCCACAGCCCAAGACCCGACCCTCTCCCAATTCGGAGGCCTACCCACTTCTCCCAGCCCCGAAAACCGCCCCACCCACAGCCCCGGCCGCACCCCCAGCCCCATCCGCGCCGGCTCTGGCGGCGACGTGCTGCTCCAGTGGGGCCACAAAAAGAGGTCCCGGGTCTCGAGAACCGAGATCCGGGTCTTGGCTGATGAGTCCTCCTCATCAGCCCAAGCAAGACAAGCCAAGGTCCAAAGGAGAGCCGCCCACGCTGCCGCCGTGGCAGACAAGTCCATGCCTCCGCCGCCTCCTCCTCCCCCAGTCCCTTCTTCCAGCTCAACGTCGTCGTTTTCGAATGGTAGACTCAGAAAGGAAGCCTCAGGGTTGCTTCCAAACAGGAATTTGGAAGATCGATCTGCTGTGGTTAATGGGTCGCCCTCGAGAAGCACTGCCGTCGGGAACGGCCGTGCTGCTTCTAGATCTATAGCTGGGAAAAGGTCTCCTCCTCCTGAGAAAAGTGAGAGAAAGATGCCATCTTGCTCGGGAAGGTCAGCCAAAGATGACAAGGCAAATGGGTCATCTGATCACCGTGCGAACCATGTCGATTCCACCTCATTACAATCGGAACAATTAGCTGGAGCTGCTAACCATTCTGCCGCACCAGCAGCTGAAAAAGTGAACTATGAAGTGGTCGAGTGGCCGAGAATTTACGTGGCTTTGTCTAGGAAGGAGAAAGAAGATGATTTCCTTGCAATGAAAGGCACGAAGCTGCCTCAGAGACCCAAGAAAAGGGCCAAGAACATTGACAGAACTCTTCAGTATTGTTTCCCAGGGATGTGGCTATCTGACTTGACAAGGAATCGCTATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGCAGAAGCGAAGGGGACTGAAAGGGATGGAGAGCGTGGAAAGTGATTCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

395

Amino Acids

43.28

Weight (kDa)

10.33

Isoelectric Point (pI)

70.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 319 - 368 2e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 75
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 598
AccII CGCG 1 cut(s) 363
AclWI GGATC 2 cut(s) 234, 427
AcoI YGGCCR 3 cut(s) 340, 700, 935
AcsI RAATTY 2 cut(s) 637, 942
AcuI CTGAAG 2 cut(s) 214, 1037
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 3 cut(s) 435, 442, 1026
AgsI TTSAA 1 cut(s) 73
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 1066
AluBI AGCT 6 cut(s) 573, 725, 872, 878, 905, 1006
AluI AGCT 6 cut(s) 573, 725, 872, 878, 905, 1006
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 3 cut(s) 424, 741, 1009
AlwI GGATC 2 cut(s) 234, 427
AlwNI CAGNNNCTG 1 cut(s) 905
Ama87I CYCGRG 4 cut(s) 414, 421, 676, 778
AoxI GGCC 6 cut(s) 292, 340, 397, 700, 935, 1029
ApeKI GCWGC 7 cut(s) 198, 385, 509, 707, 878, 902, 1006
ApoI RAATTY 2 cut(s) 637, 942
Asp718I GGTACC 1 cut(s) 87
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 4 cut(s) 397, 411, 487, 1029
AsuC2I CCSGG 4 cut(s) 339, 415, 416, 437
AsuHPI GGTGA 1 cut(s) 815
AsuII TTCGAA 1 cut(s) 590
AvaI CYCGRG 4 cut(s) 414, 421, 676, 778
AvaII GGWCC 2 cut(s) 411, 487
AxyI CCTNAGG 1 cut(s) 616
BanI GGYRCC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 7 cut(s) 210, 372, 496, 694, 865, 914, 993
BccI CCATC 3 cut(s) 365, 778, 1153
BceAI ACGGC 4 cut(s) 500, 674, 687, 715
BcgI CGANNNNNNTGC 2 cut(s) 673, 707
BciT130I CCWGG 1 cut(s) 1068
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 4 cut(s) 339, 415, 416, 437
BcoDI GTCTC 3 cut(s) 424, 741, 1009
BfaI CTAG 2 cut(s) 714, 960
BfmI CTRYAG 1 cut(s) 720
BfuAI ACCTGC 1 cut(s) 75
BglI GCCNNNNNGGC 1 cut(s) 518
BglII AGATCT 1 cut(s) 716
Bme1390I CCNGG 5 cut(s) 339, 415, 416, 437, 1068
Bme18I GGWCC 2 cut(s) 411, 487
BmeT110I CYCGRG 4 cut(s) 414, 421, 676, 778
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 4 cut(s) 397, 411, 487, 1029
BmiI GGNNCC 3 cut(s) 89, 398, 413
BmrFI CCNGG 5 cut(s) 339, 415, 416, 437, 1068
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 1 cut(s) 756
BmuI ACTGGG 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 168
BplI GAGNNNNNCTC 2 cut(s) 443, 475
BpmI CTGGAG 2 cut(s) 374, 894
Bpu14I TTCGAA 1 cut(s) 590
BpuMI CCSGG 4 cut(s) 339, 415, 416, 437
Bsa29I ATCGAT 1 cut(s) 649
BsaAI YACGTR 1 cut(s) 949
BsaBI GATNNNNATC 1 cut(s) 238
BsaI GGTCTC 3 cut(s) 424, 741, 1009
BsaJI CCNNGG 6 cut(s) 337, 414, 483, 516, 1066, 1067
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 3 cut(s) 435, 442, 1026
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 2 cut(s) 391, 557
Bse21I CCTNAGG 1 cut(s) 616
Bse3DI GCAATG 2 cut(s) 130, 996
Bse8I GATNNNNATC 1 cut(s) 238
BseBI CCWGG 1 cut(s) 1068
BseCI ATCGAT 1 cut(s) 649
BseDI CCNNGG 6 cut(s) 337, 414, 483, 516, 1066, 1067
BseGI GGATG 3 cut(s) 357, 1077, 1164
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 3 cut(s) 435, 442, 1026
BseMI GCAATG 2 cut(s) 130, 996
BseMII CTCAG 5 cut(s) 133, 616, 630, 738, 1025
BseNI ACTGG 2 cut(s) 391, 557
BseRI GAGGAG 5 cut(s) 448, 537, 540, 729, 732
BseX3I CGGCCG 2 cut(s) 340, 700
BseXI GCAGC 7 cut(s) 210, 372, 496, 694, 865, 914, 993
BseYI CCCAGC 4 cut(s) 137, 308, 350, 725
Bsh1236I CGCG 1 cut(s) 363
Bsh1285I CGRYCG 3 cut(s) 65, 343, 703
BshFI GGCC 6 cut(s) 294, 342, 399, 702, 937, 1031
BshNI GGYRCC 1 cut(s) 87
BshVI ATCGAT 1 cut(s) 649
BsiEI CGRYCG 3 cut(s) 65, 343, 703
BsiHKAI GWGCWC 1 cut(s) 151
BsiHKCI CYCGRG 4 cut(s) 414, 421, 676, 778
BsiSI CCGG 5 cut(s) 61, 339, 366, 415, 436
BslFI GGGAC 3 cut(s) 397, 545, 1161
BslI CCNNNNNNNGG 3 cut(s) 435, 442, 1026
BsmAI GTCTC 3 cut(s) 424, 741, 1009
BsmFI GGGAC 3 cut(s) 397, 545, 1161
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 6 cut(s) 294, 342, 399, 702, 937, 1031
Bso31I GGTCTC 3 cut(s) 424, 741, 1009
BsoBI CYCGRG 4 cut(s) 414, 421, 676, 778
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 6 cut(s) 239, 432, 646, 650, 716, 820
BspANI GGCC 6 cut(s) 294, 342, 399, 702, 937, 1031
BspCNI CTCAG 5 cut(s) 132, 615, 629, 739, 1024
BspDI ATCGAT 1 cut(s) 649
BspFNI CGCG 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 89, 398, 413
BspMI ACCTGC 1 cut(s) 75
BspPI GGATC 2 cut(s) 234, 427
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 87
BspTNI GGTCTC 3 cut(s) 424, 741, 1009
BsrDI GCAATG 2 cut(s) 130, 996
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 2 cut(s) 391, 557
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 6 cut(s) 337, 414, 483, 516, 1066, 1067
BssMI GATC 6 cut(s) 239, 432, 646, 650, 716, 820
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 2 cut(s) 205, 827
Bst6I CTCTTC 1 cut(s) 1056
BstBAI YACGTR 1 cut(s) 949
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 5 cut(s) 119, 602, 616, 747, 1011
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 3 cut(s) 357, 1077, 1164
BstFNI CGCG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 6 cut(s) 242, 435, 649, 653, 719, 823
BstMAI GTCTC 3 cut(s) 424, 741, 1009
BstMBI GATC 6 cut(s) 239, 432, 646, 650, 716, 820
BstMCI CGRYCG 3 cut(s) 65, 343, 703
BstNI CCWGG 1 cut(s) 1068
BstSCI CCNGG 5 cut(s) 337, 413, 414, 435, 1066
BstSFI CTRYAG 1 cut(s) 720
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 7 cut(s) 210, 372, 496, 694, 865, 914, 993
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 432, 716
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 2 cut(s) 432, 716
BstZI CGGCCG 2 cut(s) 340, 700
Bsu15I ATCGAT 1 cut(s) 649
Bsu36I CCTNAGG 1 cut(s) 616
BsuRI GGCC 6 cut(s) 294, 342, 399, 702, 937, 1031
BsuTUI ATCGAT 1 cut(s) 649
BtgI CCRYGG 1 cut(s) 516
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 3 cut(s) 357, 1077, 1164
BtsI GCAGTG 2 cut(s) 149, 684
BtsIMutI CAGTG 3 cut(s) 149, 398, 684
BveI ACCTGC 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 905
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 4 cut(s) 397, 411, 487, 1029
Cfr9I CCCGGG 1 cut(s) 414
ClaI ATCGAT 1 cut(s) 649
Csp6I GTAC 1 cut(s) 88
CviAII CATG 3 cut(s) 166, 532, 836
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 5 cut(s) 119, 602, 616, 747, 1011
DpnI GATC 6 cut(s) 241, 434, 648, 652, 718, 822
DpnII GATC 6 cut(s) 239, 432, 646, 650, 716, 820
EaeI YGGCCR 3 cut(s) 340, 700, 935
EagI CGGCCG 2 cut(s) 340, 700
Eam1104I CTCTTC 1 cut(s) 1056
EarI CTCTTC 1 cut(s) 1056
EciI GGCGGA 1 cut(s) 528
EclXI CGGCCG 2 cut(s) 340, 700
Eco130I CCWWGG 1 cut(s) 483
Eco147I AGGCCT 1 cut(s) 294
Eco31I GGTCTC 3 cut(s) 424, 741, 1009
Eco47I GGWCC 2 cut(s) 411, 487
Eco52I CGGCCG 2 cut(s) 340, 700
Eco57I CTGAAG 2 cut(s) 214, 1037
Eco81I CCTNAGG 1 cut(s) 616
Eco88I CYCGRG 4 cut(s) 414, 421, 676, 778
EcoO109I RGGNCCY 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 169, 535, 839
FaiI YATR 7 cut(s) 143, 167, 533, 722, 837, 921, 1104
FaqI GGGAC 3 cut(s) 397, 545, 1161
FatI CATG 3 cut(s) 165, 531, 835
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 598
FokI GGATG 3 cut(s) 344, 1084, 1171
FspBI CTAG 2 cut(s) 714, 960
GlaI GCGC 1 cut(s) 364
GsaI CCCAGC 4 cut(s) 141, 312, 354, 729
GsuI CTGGAG 2 cut(s) 374, 894
HaeIII GGCC 6 cut(s) 294, 342, 399, 702, 937, 1031
HapII CCGG 5 cut(s) 61, 339, 366, 415, 436
HhaI GCGC 1 cut(s) 365
Hin1II CATG 3 cut(s) 169, 535, 839
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HinfI GANTC 8 cut(s) 16, 218, 234, 452, 600, 842, 1096, 1178
HpaII CCGG 5 cut(s) 61, 339, 366, 415, 436
HphI GGTGA 1 cut(s) 815
Hpy166II GTNNAC 2 cut(s) 599, 916
Hpy188III TCNNGA 7 cut(s) 421, 423, 678, 694, 714, 746, 780
Hpy8I GTNNAC 2 cut(s) 599, 916
Hpy99I CGWCG 4 cut(s) 383, 583, 586, 695
HpyAV CCTTC 5 cut(s) 238, 573, 777, 958, 1137
HpyCH4III ACNGT 2 cut(s) 205, 827
HpyCH4IV ACGT 3 cut(s) 381, 578, 948
HpyCH4V TGCA 2 cut(s) 43, 989
HpyF3I CTNAG 5 cut(s) 119, 602, 616, 747, 1011
HpySE526I ACGT 3 cut(s) 381, 578, 948
Hsp92II CATG 3 cut(s) 169, 535, 839
HspAI GCGC 1 cut(s) 363
KpnI GGTACC 1 cut(s) 91
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 6 cut(s) 239, 432, 646, 650, 716, 820
LmnI GCTCC 2 cut(s) 393, 875
Lsp1109I GCAGC 7 cut(s) 210, 372, 496, 694, 865, 914, 993
LweI GCATC 1 cut(s) 756
MaeI CTAG 2 cut(s) 714, 960
MaeII ACGT 3 cut(s) 381, 578, 948
MalI GATC 6 cut(s) 241, 434, 648, 652, 718, 822
MboI GATC 6 cut(s) 239, 432, 646, 650, 716, 820
MboII GAAGA 8 cut(s) 173, 201, 558, 656, 986, 1043, 1129, 1141
MflI RGATCY 2 cut(s) 432, 716
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 5 cut(s) 73, 284, 637, 866, 942
MlyI GAGTC 3 cut(s) 10, 461, 594
MmeI TCCRAC 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 3 cut(s) 36, 56, 663
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 5 cut(s) 61, 339, 366, 415, 436
MspR9I CCNGG 5 cut(s) 339, 415, 416, 437, 1068
Mva1269I GAATGC 1 cut(s) 43
MvaI CCWGG 1 cut(s) 1068
MvnI CGCG 1 cut(s) 363
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 4 cut(s) 339, 415, 416, 437
NdeII GATC 6 cut(s) 239, 432, 646, 650, 716, 820
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 3 cut(s) 169, 535, 839
NlaIV GGNNCC 3 cut(s) 89, 398, 413
NmeAIII GCCGAG 1 cut(s) 963
NspV TTCGAA 1 cut(s) 590
PaeR7I CTCGAG 2 cut(s) 421, 676
PasI CCCWGGG 1 cut(s) 1067
PceI AGGCCT 1 cut(s) 294
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 587
PctI GAATGC 1 cut(s) 43
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 5 cut(s) 218, 234, 842, 1096, 1178
PleI GAGTC 3 cut(s) 10, 460, 594
PpsI GAGTC 3 cut(s) 10, 460, 594
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 1066
PspFI CCCAGC 4 cut(s) 137, 308, 350, 725
PspGI CCWGG 1 cut(s) 1066
PspN4I GGNNCC 3 cut(s) 89, 398, 413
PspPI GGNCC 4 cut(s) 397, 411, 487, 1029
PspPPI RGGWCCY 1 cut(s) 411
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 2 cut(s) 432, 716
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SaqAI TTAA 3 cut(s) 36, 56, 663
Sau3AI GATC 6 cut(s) 239, 432, 646, 650, 716, 820
Sau96I GGNCC 4 cut(s) 397, 411, 487, 1029
SchI GAGTC 3 cut(s) 10, 461, 594
ScrFI CCNGG 5 cut(s) 339, 415, 416, 437, 1068
SduI GDGCHC 1 cut(s) 151
SfaNI GCATC 1 cut(s) 756
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 2 cut(s) 421, 676
SfuI TTCGAA 1 cut(s) 590
SinI GGWCC 2 cut(s) 411, 487
SlaI CTCGAG 2 cut(s) 421, 676
SmaI CCCGGG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 421, 676
SmoI CTYRAG 2 cut(s) 421, 676
Sse9I AATT 5 cut(s) 73, 284, 637, 866, 942
SseBI AGGCCT 1 cut(s) 294
SspMI CTAG 2 cut(s) 714, 960
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 5 cut(s) 337, 413, 414, 435, 1066
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 205, 827
TaiI ACGT 3 cut(s) 384, 581, 951
TaqI TCGA 7 cut(s) 47, 422, 590, 649, 677, 840, 930
TasI AATT 5 cut(s) 73, 284, 637, 866, 942
TauI GCSGC 6 cut(s) 345, 378, 503, 515, 544, 896
TfiI GAWTC 5 cut(s) 218, 234, 842, 1096, 1178
Tru1I TTAA 3 cut(s) 36, 56, 663
Tru9I TTAA 3 cut(s) 36, 56, 663
TscAI CASTG 3 cut(s) 156, 398, 691
TseI GCWGC 7 cut(s) 198, 385, 509, 707, 878, 902, 1006
TspDTI ATGAA 3 cut(s) 17, 936, 1007
TspMI CCCGGG 1 cut(s) 414
TspRI CASTG 3 cut(s) 156, 398, 691
VpaK11BI GGWCC 2 cut(s) 411, 487
XapI RAATTY 2 cut(s) 637, 942
XbaI TCTAGA 1 cut(s) 713
XhoI CTCGAG 2 cut(s) 421, 676
XmaI CCCGGG 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 2 cut(s) 714, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.