MD10G1223600.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006362
Physical Location & Seq
Reverse (-)
32155499 .. 32160683
5185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1223600.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGATGATGAGGTACCTGAGAGTGAGCCCAGATTGCGTTCCTCTGAGCAATGGCAAGAAACCAACCGTGAGAACCATATCCAAAGACGACGGGATTACAGACACCGTGACAACAAACAGTATAGTCACATCTCTAGAACCCACCAAACCCTTCAGATTCCGATCCCAACCCACGCCTCAAGACCCGACCCAATCCCAATTCGGAACCCGACCCACTTCCCCAGACGGCGAAATCCACCACCAGACCCAGCAGCGGCTGGACAAGAGTCCCAGCCGGACTCCCAGCCCAATCCGCGGAGCCGGCGATGTGCTGCTACAGTGGGGCCAGAGAAAGAGGTCGAGAGTCTCCAGGACTGAGATCCGAGCTGTCACCGATGAGTCCTCTTCGTCGGCCCAAGCAAGGCAGGCTTCAAAGCTGCAGAGGAGAGACAAGTCCATGCCGCCTCCGCCGCCCCCGCCGCCCCTTCCGTCTTCTTCCTCCACTACGTCGTCGTTTTCTAATGGCAGCAGACCGAGAAAGGAAGCCTCCGGGTCGCTTCCTAGCAGGAATTTGGAAGATCGATCTGCCGAGGTTAATGGGTCGCCATCGAGAAACCCTACTGGTGGCAGCAACGGCCGAGCCGCTTCTAGATCTACGGCGGGTAAACGATCTCCTCCGCCGGAGAAAAATGAGAGAAAGGTGCCAGCTTGCTCAGGCAGGTCATCAGCAGCAGCCAAAAATGACAGCAAGCCAAATGGAGCTCAAGCTGATTCTACTTTGTTGCAATCGGATCAATTAGCGGCTGGTTCGGGAGCGGCGGCTAATTGTGGTGCCGCTGCCGCAGCGGAGAAGGTGAACTACGAAGTGGTGGAGTGGCCGAGAATTTATATTGCTTTGTCGAGGAAGGAGAAGGAAGATGATTTCCTTGCAATGAAAGGCACAAAGCTCCCTCAGAGACCCAAAAAAAGGGCCAAAAATATTGATAGAACTCTGCAGTATTGTTTTCCAGGGATGTGGCTATCTGACTTGACAAGGAATCGATATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGCAAAAGAGAAGGGGACTCAAGGGAATGGACAGCATCGACAGTGATTCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

369

Amino Acids

40.21

Weight (kDa)

10.22

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 292 - 341 1.8e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 687
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 3 cut(s) 12, 679, 809
AccBSI CCGCTC 1 cut(s) 794
AccII CGCG 1 cut(s) 294
AclWI GGATC 3 cut(s) 156, 352, 777
AcoI YGGCCR 2 cut(s) 613, 854
AcsI RAATTY 2 cut(s) 547, 861
AcuI CTGAAG 1 cut(s) 136
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 5 cut(s) 252, 293, 399, 602, 945
AgsI TTSAA 1 cut(s) 411
AjnI CCWGG 2 cut(s) 347, 985
AluBI AGCT 6 cut(s) 365, 415, 686, 740, 746, 925
AluI AGCT 6 cut(s) 365, 415, 686, 740, 746, 925
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 3 cut(s) 349, 420, 928
AlwI GGATC 3 cut(s) 156, 352, 777
AlwNI CAGNNNCTG 1 cut(s) 256
AoxI GGCC 5 cut(s) 322, 390, 613, 854, 948
ApeKI GCWGC 9 cut(s) 250, 310, 415, 504, 606, 707, 710, 815, 821
ApoI RAATTY 2 cut(s) 547, 861
Asp718I GGTACC 1 cut(s) 12
AspS9I GGNCC 3 cut(s) 322, 391, 948
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 2 cut(s) 361, 844
BanI GGYRCC 3 cut(s) 12, 679, 809
BanII GRGCYC 2 cut(s) 29, 742
BarI GAAGNNNNNNTAC 2 cut(s) 821, 853
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 9 cut(s) 262, 297, 402, 516, 618, 719, 722, 802, 833
BccI CCATC 1 cut(s) 592
BceAI ACGGC 3 cut(s) 241, 628, 651
BciT130I CCWGG 2 cut(s) 349, 987
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 3 cut(s) 349, 420, 928
BfaI CTAG 3 cut(s) 134, 540, 627
BfmI CTRYAG 3 cut(s) 314, 416, 971
BfuAI ACCTGC 1 cut(s) 687
BglII AGATCT 1 cut(s) 629
Bme1390I CCNGG 3 cut(s) 349, 529, 987
BmgT120I GGNCC 3 cut(s) 322, 391, 948
BmiI GGNNCC 6 cut(s) 14, 205, 298, 323, 681, 811
BmrFI CCNGG 3 cut(s) 349, 529, 987
BmsI GCATC 1 cut(s) 1095
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 691
BpuEI CTTGAG 3 cut(s) 162, 726, 1055
BpuMI CCSGG 1 cut(s) 529
Bsa29I ATCGAT 2 cut(s) 559, 1018
BsaBI GATNNNNATC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 928
BsaJI CCNNGG 3 cut(s) 292, 567, 986
Bsc4I CCNNNNNNNGG 5 cut(s) 252, 293, 399, 602, 945
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 604
Bse3DI GCAATG 2 cut(s) 55, 915
Bse8I GATNNNNATC 1 cut(s) 160
BseBI CCWGG 2 cut(s) 349, 987
BseCI ATCGAT 2 cut(s) 559, 1018
BseDI CCNNGG 3 cut(s) 292, 567, 986
BseGI GGATG 1 cut(s) 996
BseJI GATNNNNATC 1 cut(s) 160
BseLI CCNNNNNNNGG 5 cut(s) 252, 293, 399, 602, 945
BseMI GCAATG 2 cut(s) 55, 915
BseMII CTCAG 5 cut(s) 8, 35, 345, 705, 944
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 2 cut(s) 436, 642
BseX3I CGGCCG 1 cut(s) 613
BseXI GCAGC 9 cut(s) 262, 297, 402, 516, 618, 719, 722, 802, 833
BseYI CCCAGC 3 cut(s) 246, 269, 281
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 5 cut(s) 324, 392, 615, 856, 950
BshNI GGYRCC 3 cut(s) 12, 679, 809
BshVI ATCGAT 2 cut(s) 559, 1018
BsiEI CGRYCG 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 4 cut(s) 274, 300, 528, 659
BslFI GGGAC 2 cut(s) 252, 1080
BslI CCNNNNNNNGG 5 cut(s) 252, 293, 399, 602, 945
BsmAI GTCTC 3 cut(s) 349, 420, 928
BsmFI GGGAC 2 cut(s) 252, 1080
BsnI GGCC 5 cut(s) 324, 392, 615, 856, 950
Bso31I GGTCTC 1 cut(s) 928
Bsp1286I GDGCHC 2 cut(s) 29, 742
Bsp143I GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
BspANI GGCC 5 cut(s) 324, 392, 615, 856, 950
BspCNI CTCAG 5 cut(s) 9, 36, 346, 704, 943
BspDI ATCGAT 2 cut(s) 559, 1018
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 6 cut(s) 14, 205, 298, 323, 681, 811
BspMAI CTGCAG 2 cut(s) 420, 975
BspMI ACCTGC 1 cut(s) 687
BspPI GGATC 3 cut(s) 156, 352, 777
BspT107I GGYRCC 3 cut(s) 12, 679, 809
BspTNI GGTCTC 1 cut(s) 928
BsrBI CCGCTC 1 cut(s) 794
BsrDI GCAATG 2 cut(s) 55, 915
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 1 cut(s) 604
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 292, 567, 986
BssMI GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
Bst2UI CCWGG 2 cut(s) 349, 987
Bst4CI ACNGT 5 cut(s) 67, 106, 119, 318, 1094
Bst6I CTCTTC 1 cut(s) 388
BstC8I GCNNGC 5 cut(s) 301, 405, 684, 688, 728
BstDEI CTNAG 5 cut(s) 17, 44, 354, 691, 930
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 1 cut(s) 996
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 7 cut(s) 164, 360, 559, 563, 632, 650, 772
BstMAI GTCTC 3 cut(s) 349, 420, 928
BstMBI GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
BstMCI CGRYCG 1 cut(s) 616
BstNI CCWGG 2 cut(s) 349, 987
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 3 cut(s) 347, 527, 985
BstSFI CTRYAG 3 cut(s) 314, 416, 971
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 9 cut(s) 262, 297, 402, 516, 618, 719, 722, 802, 833
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 2 cut(s) 357, 629
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 2 cut(s) 357, 629
BstZI CGGCCG 1 cut(s) 613
Bsu15I ATCGAT 2 cut(s) 559, 1018
BsuRI GGCC 5 cut(s) 324, 392, 615, 856, 950
BsuTUI ATCGAT 2 cut(s) 559, 1018
BtgI CCRYGG 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 996
BtsIMutI CAGTG 2 cut(s) 323, 1099
BveI ACCTGC 1 cut(s) 687
Cac8I GCNNGC 5 cut(s) 301, 405, 684, 688, 728
CaiI CAGNNNCTG 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 3 cut(s) 322, 391, 948
Cfr42I CCGCGG 1 cut(s) 295
ClaI ATCGAT 2 cut(s) 559, 1018
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 436
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 5 cut(s) 17, 44, 354, 691, 930
DpnI GATC 7 cut(s) 163, 359, 558, 562, 631, 649, 771
DpnII GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
EaeI YGGCCR 2 cut(s) 613, 854
EagI CGGCCG 1 cut(s) 613
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
EciI GGCGGA 2 cut(s) 435, 645
Ecl136II GAGCTC 1 cut(s) 740
EclXI CGGCCG 1 cut(s) 613
Eco24I GRGCYC 2 cut(s) 29, 742
Eco31I GGTCTC 1 cut(s) 928
Eco52I CGGCCG 1 cut(s) 613
Eco53kI GAGCTC 1 cut(s) 740
Eco57I CTGAAG 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 347, 985
EcoT38I GRGCYC 2 cut(s) 29, 742
FaeI CATG 1 cut(s) 439
FaiI YATR 5 cut(s) 77, 122, 437, 867, 1023
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 2 cut(s) 252, 1080
FatI CATG 1 cut(s) 435
FauI CCCGC 2 cut(s) 462, 631
FokI GGATG 1 cut(s) 1003
FriOI GRGCYC 2 cut(s) 29, 742
FspBI CTAG 3 cut(s) 134, 540, 627
GsaI CCCAGC 3 cut(s) 250, 273, 285
GsuI CTGGAG 1 cut(s) 331
HaeIII GGCC 5 cut(s) 324, 392, 615, 856, 950
HapII CCGG 4 cut(s) 274, 300, 528, 659
Hin1II CATG 1 cut(s) 439
HinfI GANTC 9 cut(s) 156, 265, 277, 342, 377, 749, 1015, 1068, 1097
HpaII CCGG 4 cut(s) 274, 300, 528, 659
HphI GGTGA 2 cut(s) 361, 844
Hpy166II GTNNAC 2 cut(s) 644, 835
Hpy188I TCNGA 9 cut(s) 45, 155, 161, 203, 362, 769, 933, 1003, 1102
Hpy188III TCNNGA 6 cut(s) 134, 179, 339, 588, 627, 789
Hpy8I GTNNAC 2 cut(s) 644, 835
Hpy99I CGWCG 4 cut(s) 92, 391, 490, 493
HpyAV CCTTC 6 cut(s) 160, 473, 823, 877, 883, 1056
HpyCH4III ACNGT 5 cut(s) 67, 106, 119, 318, 1094
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 4 cut(s) 418, 763, 908, 973
HpyF3I CTNAG 5 cut(s) 17, 44, 354, 691, 930
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 1 cut(s) 439
KpnI GGTACC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 295
Kzo9I GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
LmnI GCTCC 4 cut(s) 296, 737, 791, 930
Lsp1109I GCAGC 9 cut(s) 262, 297, 402, 516, 618, 719, 722, 802, 833
LweI GCATC 1 cut(s) 1095
MaeI CTAG 3 cut(s) 134, 540, 627
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 3 cut(s) 106, 124, 367
MalI GATC 7 cut(s) 163, 359, 558, 562, 631, 649, 771
MbiI CCGCTC 1 cut(s) 794
MboI GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
MboII GAAGA 7 cut(s) 375, 462, 465, 566, 905, 1048, 1060
MflI RGATCY 2 cut(s) 357, 629
MhlI GDGCHC 2 cut(s) 29, 742
MluCI AATT 5 cut(s) 197, 547, 773, 802, 861
MlyI GAGTC 5 cut(s) 271, 274, 351, 386, 1062
MroNI GCCGGC 1 cut(s) 299
MseI TTAA 1 cut(s) 573
MspA1I CMGCKG 4 cut(s) 253, 294, 815, 824
MspI CCGG 4 cut(s) 274, 300, 528, 659
MspR9I CCNGG 3 cut(s) 349, 529, 987
MvaI CCWGG 2 cut(s) 349, 987
MvnI CGCG 1 cut(s) 294
NaeI GCCGGC 1 cut(s) 301
NciI CCSGG 1 cut(s) 529
NdeII GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 6 cut(s) 14, 205, 298, 323, 681, 811
NmeAIII GCCGAG 3 cut(s) 592, 641, 882
NmuCI GTSAC 3 cut(s) 106, 124, 367
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 4 cut(s) 156, 749, 1015, 1097
PfoI TCCNGGA 1 cut(s) 347
PleI GAGTC 5 cut(s) 271, 273, 350, 385, 1062
PpsI GAGTC 5 cut(s) 271, 273, 350, 385, 1062
PshAI GACNNNNGTC 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 742
Psp6I CCWGG 2 cut(s) 347, 985
PspFI CCCAGC 3 cut(s) 246, 269, 281
PspGI CCWGG 2 cut(s) 347, 985
PspN4I GGNNCC 6 cut(s) 14, 205, 298, 323, 681, 811
PspPI GGNCC 3 cut(s) 322, 391, 948
PstI CTGCAG 2 cut(s) 420, 975
PstNI CAGNNNCTG 1 cut(s) 256
PsuI RGATCY 2 cut(s) 357, 629
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SacI GAGCTC 1 cut(s) 742
SacII CCGCGG 1 cut(s) 295
SaqAI TTAA 1 cut(s) 573
Sau3AI GATC 7 cut(s) 161, 357, 556, 560, 629, 647, 769
Sau96I GGNCC 3 cut(s) 322, 391, 948
SchI GAGTC 5 cut(s) 271, 274, 351, 386, 1062
ScrFI CCNGG 3 cut(s) 349, 529, 987
SduI GDGCHC 2 cut(s) 29, 742
SfaNI GCATC 1 cut(s) 1095
SfcI CTRYAG 3 cut(s) 314, 416, 971
Sfr303I CCGCGG 1 cut(s) 295
SgrBI CCGCGG 1 cut(s) 295
SmlI CTYRAG 3 cut(s) 177, 741, 1070
SmoI CTYRAG 3 cut(s) 177, 741, 1070
Sse9I AATT 5 cut(s) 197, 547, 773, 802, 861
SspI AATATT 1 cut(s) 958
SspMI CTAG 3 cut(s) 134, 540, 627
SstI GAGCTC 1 cut(s) 742
StyD4I CCNGG 3 cut(s) 347, 527, 985
TaaI ACNGT 5 cut(s) 67, 106, 119, 318, 1094
TaiI ACGT 1 cut(s) 488
TaqI TCGA 6 cut(s) 338, 559, 587, 878, 1018, 1089
TaqII GACCGA 1 cut(s) 526
TasI AATT 5 cut(s) 197, 547, 773, 802, 861
TfiI GAWTC 4 cut(s) 156, 749, 1015, 1097
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 2 cut(s) 323, 1099
TseFI GTSAC 3 cut(s) 106, 124, 367
TseI GCWGC 9 cut(s) 250, 310, 415, 504, 606, 707, 710, 815, 821
Tsp45I GTSAC 3 cut(s) 106, 124, 367
TspDTI ATGAA 1 cut(s) 926
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 323, 1099
XapI RAATTY 2 cut(s) 547, 861
XbaI TCTAGA 2 cut(s) 133, 626
XspI CTAG 3 cut(s) 134, 540, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.