Rh5DG159400

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006362
Physical Location & Seq
Forward (+)
16296508 .. 16301229
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG159400.1

Sequence Viewer

Length: 1044 bp
ATGATGACAGAGAACACAACAACCACAACAAGCAGCACAGTTTCTTCGTTGGAATCCAAACCCTTCAGATTCCGATCCCAACCCACGGCCCAAGACCCGACCCTCTCCCAATTCGGAGGCCTACCCACTTCTCCCAGCCCCGAAAACCGCCCCACCCACAGCCCCGGCCGCACCCCCAGCCCCATCCGCGCCGGCTCCGGCGGCGACGTGCTGCTCCAGTGGGGCCACAAAAAGAGGTCCCGGGTCTCGAGAACCGAGATCCGGGTCTTGGCTGATGAGTCCTCCTCATCAGCCCAAGCAAGACAAGCCAAGGTCCAAAGGCGAGCCGCCCACGCTGCCGCCGTGGCAGACAAGTCCATGCCTCCGCCGCCTCCTCCCCCCCCGGTCCCTTCTTCCAGCTCAACGTCGTCGTTTTCGAATGGTAGACTCAGAAAGGAAGCCTCAGGGTTGCTTCCAAACAGGAATTTGGAAGATCGATCCGCTGTGGTTAATGGGTCACCCTCGAGAAGCACTGCCGTCGGGAACGGTCGTGCTGCTTCTAGATCTATAGCTGGGAAAAGGTCTCCTCCTCCAGAGAAAAGTGAGAGAAAGATGCCATCTTGCTCGGGAAGGTCAGCCAAAGATGACAAGGCAAATGGGTCATCTGATCACCGTGCGAACCATGTCGATTCCACCTCATTACAATCGGAACAATTAGCTGGTGCTGCTAACCATTCTGCCGCACCAGCAGCTGAAAAAGTGAACTATGAAGTGGTGGAGTGGCCGAGAATTTACGTGGCTTTGTCTAGGAAGGAGAAAGAAGATGATTTCCTTGCAATGAAAGGCACGAAGCTGCCTCAGAGACCCAAGAAAAGGGCCAAGAACATTGACAGAACTCTTCAGTATTGTTTCCCAGGGATGTGGCTATCTGACTTGACAAGGAATCGCTATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGGTAAATGCTGTGCCCTCTGCTCTTTCTTGCCTCTTTGTCCTTTCTTTGTTTTGGATAATATCTTTAGCTCTAATGCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

347

Amino Acids

37.68

Weight (kDa)

10.36

Isoelectric Point (pI)

70.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 261 - 310 1.7e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 424
AccII CGCG 1 cut(s) 189
AclWI GGATC 3 cut(s) 69, 253, 471
AcoI YGGCCR 2 cut(s) 166, 761
AcsI RAATTY 2 cut(s) 463, 768
AcuI CTGAAG 2 cut(s) 49, 863
AfiI CCNNNNNNNGG 4 cut(s) 85, 261, 268, 852
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 892
AluBI AGCT 6 cut(s) 399, 551, 698, 731, 832, 1028
AluI AGCT 6 cut(s) 399, 551, 698, 731, 832, 1028
Alw26I GTCTC 3 cut(s) 250, 567, 835
AlwI GGATC 3 cut(s) 69, 253, 471
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 4 cut(s) 240, 247, 502, 604
AoxI GGCC 6 cut(s) 87, 118, 166, 223, 761, 855
ApeKI GCWGC 7 cut(s) 33, 211, 335, 533, 704, 728, 832
ApoI RAATTY 2 cut(s) 463, 768
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 6 cut(s) 88, 223, 237, 313, 385, 855
AsuC2I CCSGG 5 cut(s) 165, 241, 242, 263, 383
AsuHPI GGTGA 2 cut(s) 489, 641
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 4 cut(s) 240, 247, 502, 604
AvaII GGWCC 3 cut(s) 237, 313, 385
AxyI CCTNAGG 1 cut(s) 442
BaeGI GKGCMC 1 cut(s) 975
BbvI GCAGC 7 cut(s) 45, 198, 322, 520, 691, 740, 819
BccI CCATC 2 cut(s) 191, 604
BceAI ACGGC 3 cut(s) 102, 326, 500
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 1 cut(s) 894
BclI TGATCA 1 cut(s) 646
BcnI CCSGG 5 cut(s) 165, 241, 242, 263, 383
BcoDI GTCTC 3 cut(s) 250, 567, 835
BfaI CTAG 2 cut(s) 540, 786
BfmI CTRYAG 1 cut(s) 546
BglI GCCNNNNNGGC 1 cut(s) 344
BglII AGATCT 1 cut(s) 542
Bme1390I CCNGG 6 cut(s) 165, 241, 242, 263, 383, 894
Bme18I GGWCC 3 cut(s) 237, 313, 385
BmeT110I CYCGRG 4 cut(s) 240, 247, 502, 604
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 6 cut(s) 88, 223, 237, 313, 385, 855
BmiI GGNNCC 4 cut(s) 196, 224, 239, 387
BmrFI CCNGG 6 cut(s) 165, 241, 242, 263, 383, 894
BmsI GCATC 1 cut(s) 582
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpmI CTGGAG 2 cut(s) 200, 555
Bpu14I TTCGAA 1 cut(s) 416
BpuMI CCSGG 5 cut(s) 165, 241, 242, 263, 383
Bsa29I ATCGAT 1 cut(s) 475
BsaAI YACGTR 1 cut(s) 775
BsaBI GATNNNNATC 1 cut(s) 73
BsaI GGTCTC 3 cut(s) 250, 567, 835
BsaJI CCNNGG 8 cut(s) 84, 163, 240, 309, 342, 381, 892, 893
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 261, 268, 852
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 217
Bse21I CCTNAGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 1 cut(s) 894
BseCI ATCGAT 1 cut(s) 475
BseDI CCNNGG 8 cut(s) 84, 163, 240, 309, 342, 381, 892, 893
BseGI GGATG 2 cut(s) 183, 903
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 4 cut(s) 85, 261, 268, 852
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 3 cut(s) 442, 456, 851
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 4 cut(s) 274, 363, 555, 558
BseSI GKGCMC 1 cut(s) 975
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 7 cut(s) 45, 198, 322, 520, 691, 740, 819
BseYI CCCAGC 3 cut(s) 134, 176, 551
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 2 cut(s) 169, 529
BshFI GGCC 6 cut(s) 89, 120, 168, 225, 763, 857
BshVI ATCGAT 1 cut(s) 475
BsiEI CGRYCG 2 cut(s) 169, 529
BsiHKCI CYCGRG 4 cut(s) 240, 247, 502, 604
BsiSI CCGG 6 cut(s) 165, 192, 198, 241, 262, 383
BslFI GGGAC 2 cut(s) 223, 371
BslI CCNNNNNNNGG 4 cut(s) 85, 261, 268, 852
BsmAI GTCTC 3 cut(s) 250, 567, 835
BsmFI GGGAC 2 cut(s) 223, 371
BsnI GGCC 6 cut(s) 89, 120, 168, 225, 763, 857
Bso31I GGTCTC 3 cut(s) 250, 567, 835
BsoBI CYCGRG 4 cut(s) 240, 247, 502, 604
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 975
Bsp143I GATC 6 cut(s) 74, 258, 472, 476, 542, 646
BspANI GGCC 6 cut(s) 89, 120, 168, 225, 763, 857
BspCNI CTCAG 3 cut(s) 441, 455, 850
BspDI ATCGAT 1 cut(s) 475
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 4 cut(s) 196, 224, 239, 387
BspPI GGATC 3 cut(s) 69, 253, 471
BspT104I TTCGAA 1 cut(s) 416
BspTNI GGTCTC 3 cut(s) 250, 567, 835
BsrDI GCAATG 1 cut(s) 822
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 8 cut(s) 84, 163, 240, 309, 342, 381, 892, 893
BssMI GATC 6 cut(s) 74, 258, 472, 476, 542, 646
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 3 cut(s) 40, 527, 653
Bst6I CTCTTC 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 775
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 193, 324
BstDEI CTNAG 3 cut(s) 428, 442, 837
BstDSI CCRYGG 2 cut(s) 84, 342
BstEII GGTNACC 1 cut(s) 495
BstF5I GGATG 2 cut(s) 183, 903
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 6 cut(s) 77, 261, 475, 479, 545, 649
BstMAI GTCTC 3 cut(s) 250, 567, 835
BstMBI GATC 6 cut(s) 74, 258, 472, 476, 542, 646
BstMCI CGRYCG 2 cut(s) 169, 529
BstNI CCWGG 1 cut(s) 894
BstPI GGTNACC 1 cut(s) 495
BstSCI CCNGG 6 cut(s) 163, 239, 240, 261, 381, 892
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 1 cut(s) 975
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 7 cut(s) 45, 198, 322, 520, 691, 740, 819
BstX2I RGATCY 2 cut(s) 258, 542
BstXI CCANNNNNNTGG 1 cut(s) 900
BstYI RGATCY 2 cut(s) 258, 542
BstZI CGGCCG 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 442
BsuRI GGCC 6 cut(s) 89, 120, 168, 225, 763, 857
BsuTUI ATCGAT 1 cut(s) 475
BtgI CCRYGG 2 cut(s) 84, 342
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 2 cut(s) 183, 903
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 224, 510
Cac8I GCNNGC 2 cut(s) 193, 324
CaiI CAGNNNCTG 1 cut(s) 731
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 6 cut(s) 88, 223, 237, 313, 385, 855
Cfr9I CCCGGG 1 cut(s) 240
ClaI ATCGAT 1 cut(s) 475
CviAII CATG 2 cut(s) 358, 662
DdeI CTNAG 3 cut(s) 428, 442, 837
DpnI GATC 6 cut(s) 76, 260, 474, 478, 544, 648
DpnII GATC 6 cut(s) 74, 258, 472, 476, 542, 646
EaeI YGGCCR 2 cut(s) 166, 761
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
EciI GGCGGA 1 cut(s) 354
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 120
Eco31I GGTCTC 3 cut(s) 250, 567, 835
Eco47I GGWCC 3 cut(s) 237, 313, 385
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 49, 863
Eco81I CCTNAGG 1 cut(s) 442
Eco88I CYCGRG 4 cut(s) 240, 247, 502, 604
Eco91I GGTNACC 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 237
EcoO65I GGTNACC 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 892
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 2 cut(s) 361, 665
FaiI YATR 5 cut(s) 359, 548, 663, 747, 930
FaqI GGGAC 2 cut(s) 223, 371
FatI CATG 2 cut(s) 357, 661
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 424
FokI GGATG 2 cut(s) 170, 910
FspBI CTAG 2 cut(s) 540, 786
GlaI GCGC 1 cut(s) 190
GsaI CCCAGC 3 cut(s) 138, 180, 555
GsuI CTGGAG 2 cut(s) 200, 555
HaeIII GGCC 6 cut(s) 89, 120, 168, 225, 763, 857
HapII CCGG 6 cut(s) 165, 192, 198, 241, 262, 383
HhaI GCGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 361, 665
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 6 cut(s) 53, 69, 278, 426, 668, 922
HpaII CCGG 6 cut(s) 165, 192, 198, 241, 262, 383
HphI GGTGA 2 cut(s) 489, 641
Hpy166II GTNNAC 2 cut(s) 425, 742
Hpy188I TCNGA 8 cut(s) 68, 74, 116, 431, 646, 688, 840, 910
Hpy188III TCNNGA 7 cut(s) 247, 249, 504, 520, 540, 572, 606
Hpy8I GTNNAC 2 cut(s) 425, 742
Hpy99I CGWCG 4 cut(s) 209, 409, 412, 521
HpyAV CCTTC 5 cut(s) 73, 399, 603, 784, 952
HpyCH4III ACNGT 3 cut(s) 40, 527, 653
HpyCH4IV ACGT 3 cut(s) 207, 404, 774
HpyCH4V TGCA 1 cut(s) 815
HpyF3I CTNAG 3 cut(s) 428, 442, 837
HpySE526I ACGT 3 cut(s) 207, 404, 774
Hsp92II CATG 2 cut(s) 361, 665
HspAI GCGC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 6 cut(s) 74, 258, 472, 476, 542, 646
LmnI GCTCC 2 cut(s) 200, 219
Lsp1109I GCAGC 7 cut(s) 45, 198, 322, 520, 691, 740, 819
LweI GCATC 1 cut(s) 582
MaeI CTAG 2 cut(s) 540, 786
MaeII ACGT 3 cut(s) 207, 404, 774
MaeIII GTNAC 1 cut(s) 495
MalI GATC 6 cut(s) 76, 260, 474, 478, 544, 648
MboI GATC 6 cut(s) 74, 258, 472, 476, 542, 646
MboII GAAGA 7 cut(s) 36, 384, 482, 812, 869, 955, 967
MflI RGATCY 2 cut(s) 258, 542
MhlI GDGCHC 1 cut(s) 975
MluCI AATT 4 cut(s) 110, 463, 692, 768
MlyI GAGTC 2 cut(s) 287, 420
MmeI TCCRAC 1 cut(s) 30
MroNI GCCGGC 1 cut(s) 191
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 482, 731
MspI CCGG 6 cut(s) 165, 192, 198, 241, 262, 383
MspR9I CCNGG 6 cut(s) 165, 241, 242, 263, 383, 894
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 1 cut(s) 189
NaeI GCCGGC 1 cut(s) 193
NciI CCSGG 5 cut(s) 165, 241, 242, 263, 383
NdeII GATC 6 cut(s) 74, 258, 472, 476, 542, 646
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 2 cut(s) 361, 665
NlaIV GGNNCC 4 cut(s) 196, 224, 239, 387
NmeAIII GCCGAG 1 cut(s) 789
NmuCI GTSAC 1 cut(s) 495
NspV TTCGAA 1 cut(s) 416
PaeR7I CTCGAG 2 cut(s) 247, 502
PasI CCCWGGG 1 cut(s) 893
PceI AGGCCT 1 cut(s) 120
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 413
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 4 cut(s) 53, 69, 668, 922
PleI GAGTC 2 cut(s) 286, 420
PpsI GAGTC 2 cut(s) 286, 420
Ppu21I YACGTR 1 cut(s) 775
PpuMI RGGWCCY 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 892
PspEI GGTNACC 1 cut(s) 495
PspFI CCCAGC 3 cut(s) 134, 176, 551
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 4 cut(s) 196, 224, 239, 387
PspPI GGNCC 6 cut(s) 88, 223, 237, 313, 385, 855
PspPPI RGGWCCY 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 2 cut(s) 258, 542
PvuII CAGCTG 1 cut(s) 731
SaqAI TTAA 1 cut(s) 489
Sau3AI GATC 6 cut(s) 74, 258, 472, 476, 542, 646
Sau96I GGNCC 6 cut(s) 88, 223, 237, 313, 385, 855
SchI GAGTC 2 cut(s) 287, 420
ScrFI CCNGG 6 cut(s) 165, 241, 242, 263, 383, 894
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 1 cut(s) 582
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 2 cut(s) 247, 502
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 3 cut(s) 237, 313, 385
SlaI CTCGAG 2 cut(s) 247, 502
SmaI CCCGGG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 247, 502
SmoI CTYRAG 2 cut(s) 247, 502
Sse9I AATT 4 cut(s) 110, 463, 692, 768
SseBI AGGCCT 1 cut(s) 120
SspMI CTAG 2 cut(s) 540, 786
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 6 cut(s) 163, 239, 240, 261, 381, 892
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 40, 527, 653
TaiI ACGT 3 cut(s) 210, 407, 777
TaqI TCGA 5 cut(s) 248, 416, 475, 503, 666
TasI AATT 4 cut(s) 110, 463, 692, 768
TauI GCSGC 6 cut(s) 171, 204, 329, 341, 370, 722
TfiI GAWTC 4 cut(s) 53, 69, 668, 922
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 2 cut(s) 224, 517
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 7 cut(s) 33, 211, 335, 533, 704, 728, 832
Tsp45I GTSAC 1 cut(s) 495
TspDTI ATGAA 2 cut(s) 762, 833
TspMI CCCGGG 1 cut(s) 240
TspRI CASTG 2 cut(s) 224, 517
VpaK11BI GGWCC 3 cut(s) 237, 313, 385
XapI RAATTY 2 cut(s) 463, 768
XbaI TCTAGA 1 cut(s) 539
XhoI CTCGAG 2 cut(s) 247, 502
XmaI CCCGGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 424
XspI CTAG 2 cut(s) 540, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.