AT4G24715

Nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004983
Physical Location & Seq
Reverse (-)
12749278 .. 12750997
1720 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24715.1

Sequence Viewer

Length: 696 bp
ATGAGACACATGTGTGACACACATTTAGTCGTCCTCCCTTACCAAGATCACATTCTCATTCGGATCATGGCCATCCACACTCTCCTCTTCGTATCACTTCTCATATTCTCACTCATCGAGTCGAGTAGTGGAGGAAAGAAAGATAGACTCTTCACCGATCTCCAAAACTCAATTGAGGTCACCGCTAAACCCGTTAAAGATAGCGGAGTTTTAGAGGCTGGAAAAGATATGGTGACAATTACATGGAAGCTAAAGTCGTCGTCAGCCAAGGTCGACACGGATACTGCCTTCAAGACAATCCAAGTCAAGCTTTGTTATGCACCGATCAGCCAAGTTGATAGACCGTGGCGTAAGACTGATAACAAGCTCTTCAAGGACAGAAGTTGCCCTCATGAGATTGTGTCCAAGGCATACGACAAGACCCCCCAATCACTCGATTGGACAATCGGACTTGATATCCCCACTGGAACCTACTTCGTACGTGCCTATGGAATCGATGGAGATGGCCACGAAGTTGCATACGGACAGAGCACGGATGAGGGAAGGACGACAAATCTCTTCAGTGTTCATGCCATAAGTGGTCATCACGTGGGGCTAGACATAGCATCCACATTTTTCAGCGTCTTCTCCGTTGTTTCTCTGTTTGTCTTCTTTGTCATGGAGAAGAGGAAGGCCAAGTTAGAGCAAAGGGAGTGA

Protein Analysis

231

Amino Acids

25.99

Weight (kDa)

7.76

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 51 - 194 5.1e-58 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AciI CCGC 2 cut(s) 183, 204
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 2 cut(s) 69, 505
AcuI CTGAAG 1 cut(s) 544
AcvI CACGTG 1 cut(s) 589
AfaI GTAC 1 cut(s) 480
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 2 cut(s) 292, 373
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 3 cut(s) 250, 310, 367
AluI AGCT 3 cut(s) 250, 310, 367
Alw21I GWGCWC 1 cut(s) 533
AlwI GGATC 1 cut(s) 71
AoxI GGCC 3 cut(s) 69, 505, 672
AsuHPI GGTGA 3 cut(s) 145, 172, 244
BalI TGGCCA 2 cut(s) 71, 507
BbrPI CACGTG 1 cut(s) 589
BbsI GAAGAC 2 cut(s) 616, 640
Bbv12I GWGCWC 1 cut(s) 533
BccI CCATC 3 cut(s) 80, 491, 497
BciVI GTATCC 1 cut(s) 274
BfaI CTAG 1 cut(s) 596
BfuI GTATCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 469
BmsI GCATC 1 cut(s) 614
BpiI GAAGAC 2 cut(s) 616, 640
Bsa29I ATCGAT 1 cut(s) 495
BsaAI YACGTR 2 cut(s) 482, 589
BsaJI CCNNGG 3 cut(s) 267, 344, 405
Bse1I ACTGG 1 cut(s) 469
BseCI ATCGAT 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 267, 344, 405
BseGI GGATG 3 cut(s) 72, 541, 605
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 1 cut(s) 74
BshFI GGCC 3 cut(s) 71, 507, 674
BshVI ATCGAT 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 533
BsiWI CGTACG 1 cut(s) 478
BsnI GGCC 3 cut(s) 71, 507, 674
Bsp1286I GDGCHC 1 cut(s) 533
Bsp143I GATC 4 cut(s) 46, 63, 157, 324
BspACI CCGC 2 cut(s) 183, 204
BspANI GGCC 3 cut(s) 71, 507, 674
BspDI ATCGAT 1 cut(s) 495
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 469
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 1 cut(s) 374
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 267, 344, 405
BssMI GATC 4 cut(s) 46, 63, 157, 324
BssT1I CCWWGG 2 cut(s) 267, 405
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 5 cut(s) 92, 155, 374, 563, 659
BstBAI YACGTR 2 cut(s) 482, 589
BstDSI CCRYGG 1 cut(s) 344
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 3 cut(s) 72, 541, 605
BstKTI GATC 4 cut(s) 49, 66, 160, 327
BstMBI GATC 4 cut(s) 46, 63, 157, 324
BstNSI RCATGY 1 cut(s) 13
BstPI GGTNACC 1 cut(s) 178
BstV2I GAAGAC 2 cut(s) 616, 640
Bsu15I ATCGAT 1 cut(s) 495
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 3 cut(s) 71, 507, 674
BsuTUI ATCGAT 1 cut(s) 495
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 3 cut(s) 72, 541, 605
BtsIMutI CAGTG 2 cut(s) 462, 568
CciI TCATGA 1 cut(s) 391
ClaI ATCGAT 1 cut(s) 495
CseI GACGC 1 cut(s) 610
Csp6I GTAC 1 cut(s) 479
CviAII CATG 6 cut(s) 10, 67, 243, 392, 569, 658
CviQI GTAC 1 cut(s) 479
DpnI GATC 4 cut(s) 48, 65, 159, 326
DpnII GATC 4 cut(s) 46, 63, 157, 324
EaeI YGGCCR 2 cut(s) 69, 505
Eam1104I CTCTTC 5 cut(s) 92, 155, 374, 563, 659
EarI CTCTTC 5 cut(s) 92, 155, 374, 563, 659
Eco130I CCWWGG 2 cut(s) 267, 405
Eco32I GATATC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 544
Eco72I CACGTG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 2 cut(s) 267, 405
ErhI CCWWGG 2 cut(s) 267, 405
FaeI CATG 6 cut(s) 13, 70, 246, 395, 572, 661
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 6 cut(s) 9, 66, 242, 391, 568, 657
FblI GTMKAC 1 cut(s) 273
FokI GGATG 3 cut(s) 59, 548, 592
FspBI CTAG 1 cut(s) 596
HaeIII GGCC 3 cut(s) 71, 507, 674
HgaI GACGC 1 cut(s) 610
Hin1II CATG 6 cut(s) 13, 70, 246, 395, 572, 661
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 3 cut(s) 119, 147, 492
HphI GGTGA 3 cut(s) 145, 172, 244
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 63, 449
Hpy188III TCNNGA 2 cut(s) 292, 392
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 3 cut(s) 298, 537, 664
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 2 cut(s) 481, 588
HpyCH4V TGCA 2 cut(s) 320, 518
HpySE526I ACGT 2 cut(s) 481, 588
Hsp92II CATG 6 cut(s) 13, 70, 246, 395, 572, 661
Kzo9I GATC 4 cut(s) 46, 63, 157, 324
LguI GCTCTTC 1 cut(s) 374
LpnPI CCDG 2 cut(s) 204, 450
LweI GCATC 1 cut(s) 614
MaeI CTAG 1 cut(s) 596
MaeII ACGT 2 cut(s) 481, 588
MaeIII GTNAC 3 cut(s) 14, 178, 232
MalI GATC 4 cut(s) 48, 65, 159, 326
MboI GATC 4 cut(s) 46, 63, 157, 324
MboII GAAGA 7 cut(s) 79, 142, 361, 550, 616, 640, 676
MfeI CAATTG 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 533
MlsI TGGCCA 2 cut(s) 71, 507
MluCI AATT 2 cut(s) 171, 237
MluNI TGGCCA 2 cut(s) 71, 507
MlyI GAGTC 2 cut(s) 128, 141
MnlI CCTC 8 cut(s) 44, 95, 125, 169, 208, 399, 532, 660
Mox20I TGGCCA 2 cut(s) 71, 507
MscI TGGCCA 2 cut(s) 71, 507
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 2 cut(s) 71, 507
MunI CAATTG 1 cut(s) 171
NdeII GATC 4 cut(s) 46, 63, 157, 324
NlaIII CATG 6 cut(s) 13, 70, 246, 395, 572, 661
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 3 cut(s) 14, 178, 232
NspI RCATGY 1 cut(s) 13
OliI CACNNNNGTG 1 cut(s) 12
PagI TCATGA 1 cut(s) 391
PciI ACATGT 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 492
Pfl23II CGTACG 1 cut(s) 478
PleI GAGTC 2 cut(s) 127, 141
PmaCI CACGTG 1 cut(s) 589
PmlI CACGTG 1 cut(s) 589
PpsI GAGTC 2 cut(s) 127, 141
Ppu21I YACGTR 2 cut(s) 482, 589
PscI ACATGT 1 cut(s) 9
PspCI CACGTG 1 cut(s) 589
PspEI GGTNACC 1 cut(s) 178
PspLI CGTACG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 469
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 12
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 1 cut(s) 195
Sau3AI GATC 4 cut(s) 46, 63, 157, 324
SchI GAGTC 2 cut(s) 128, 141
SduI GDGCHC 1 cut(s) 533
SetI ASST 8 cut(s) 180, 252, 273, 312, 369, 473, 484, 591
SfaNI GCATC 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 2 cut(s) 171, 237
SsiI CCGC 2 cut(s) 183, 204
SspMI CTAG 1 cut(s) 596
StyI CCWWGG 2 cut(s) 267, 405
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 2 cut(s) 484, 591
TaqI TCGA 5 cut(s) 117, 122, 273, 435, 495
TasI AATT 2 cut(s) 171, 237
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 2 cut(s) 469, 568
TseFI GTSAC 3 cut(s) 14, 178, 232
Tsp45I GTSAC 3 cut(s) 14, 178, 232
TspDTI ATGAA 1 cut(s) 557
TspGWI ACGGA 4 cut(s) 293, 537, 548, 619
TspRI CASTG 2 cut(s) 469, 568
XceI RCATGY 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.