RchiOBHm_Chr7g0192401

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004983
Physical Location & Seq
Reverse (-)
10896550 .. 10898035
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17197

Sequence Viewer

Length: 633 bp
ATGGCTTCACGTGCTGGGATTCTATTGGCTTCTGTGCTTCTCCTCTCTTGCTTAGCAGAAACCTCCTATGGTGTTCTCTTCTCCACTCTGAAACAATCTCTTATTGTAACTGCTTCACACGTTGAGGCAAATGGAACACTCCTGAAAGCTGGGGAAGACAAAATCTCTGTTACATGGGGTTTGAACCAGAGCCTCCCAGCTGGGACTGACTCGGCCTACAAGACCGTCACAGTGAAGCTCTGCTACGCGCCGGTGAGCCAAGTCGACCGCGCGTGGAGGAAGACAGTGGACAACCTCGTCAAGGACAAGACCTGTCAGCACAAGATCGTCGCCAGGCCGTACAACGCCTCCATGCAGACCTTCGAGTGGACAGTCGAGCGTGACGTGCCCACCGCCAAGTACTTCGTACGCGCCTACGCCTACGACGCCGTCGAGGTGGCGGTGGCTTACGGTCAAAACACGGGACCTAAGAAGGACTCCAACTTGTTTGAGGTGCAGTCTATCACCGGGCGCCACGTGACGCTTGATATTGTTTCCGTCTGCTTCAGCGCTTTCTCGGTTTTAGCCCTGGCTGGGTTCTTCATTGCTGAGAAGACGAGGGCAAAGTCGTCCAAAAGAAGTGTTGGTAACTAG

Protein Analysis

210

Amino Acids

22.84

Weight (kDa)

9.25

Isoelectric Point (pI)

25.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 27 - 170 6.2e-61 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 510
AccI GTMKAC 1 cut(s) 264
AccII CGCG 4 cut(s) 248, 270, 272, 411
AciI CCGC 3 cut(s) 268, 393, 440
AcuI CTGAAG 1 cut(s) 529
AcvI CACGTG 2 cut(s) 11, 517
AcyI GRCGYC 2 cut(s) 426, 511
AfaI GTAC 3 cut(s) 341, 401, 408
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 3 cut(s) 301, 366, 573
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 1 cut(s) 184
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 332, 567
AluBI AGCT 3 cut(s) 149, 200, 238
AluI AGCT 3 cut(s) 149, 200, 238
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 2 cut(s) 213, 335
AspLEI GCGC 5 cut(s) 250, 272, 413, 513, 551
AspS9I GGNCC 1 cut(s) 464
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 265, 496
AvaII GGWCC 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 390
BanI GGYRCC 1 cut(s) 510
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbrPI CACGTG 2 cut(s) 11, 517
BbsI GAAGAC 3 cut(s) 162, 287, 599
BceAI ACGGC 2 cut(s) 322, 413
BciT130I CCWGG 2 cut(s) 334, 569
BcnI CCSGG 1 cut(s) 508
BfaI CTAG 1 cut(s) 631
BfoI RGCGCY 2 cut(s) 514, 552
BlpI GCTNAGC 1 cut(s) 52
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 3 cut(s) 334, 508, 569
Bme18I GGWCC 1 cut(s) 464
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 465, 512
BmrFI CCNGG 3 cut(s) 334, 508, 569
BpiI GAAGAC 3 cut(s) 162, 287, 599
Bpu1102I GCTNAGC 1 cut(s) 52
BpuMI CCSGG 1 cut(s) 508
BsaAI YACGTR 2 cut(s) 11, 517
BsaHI GRCGYC 2 cut(s) 426, 511
BsaJI CCNNGG 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 366, 573
Bse118I RCCGGY 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 582
BseBI CCWGG 2 cut(s) 334, 569
BseDI CCNNGG 1 cut(s) 567
BseLI CCNNNNNNNGG 3 cut(s) 301, 366, 573
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 1 cut(s) 579
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 390
BseYI CCCAGC 5 cut(s) 14, 149, 196, 200, 572
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 4 cut(s) 248, 270, 272, 411
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 2 cut(s) 215, 337
BshNI GGYRCC 1 cut(s) 510
BsiEI CGRYCG 1 cut(s) 268
BsiSI CCGG 2 cut(s) 251, 507
BsiWI CGTACG 1 cut(s) 406
BslFI GGGAC 2 cut(s) 217, 477
BslI CCNNNNNNNGG 3 cut(s) 301, 366, 573
BsmFI GGGAC 2 cut(s) 217, 477
BsnI GGCC 2 cut(s) 215, 337
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 324
Bsp1720I GCTNAGC 1 cut(s) 52
BspACI CCGC 3 cut(s) 268, 393, 440
BspANI GGCC 2 cut(s) 215, 337
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 4 cut(s) 248, 270, 272, 411
BspLI GGNNCC 2 cut(s) 465, 512
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 1 cut(s) 324
BssNI GRCGYC 2 cut(s) 426, 511
Bst2UI CCWGG 2 cut(s) 334, 569
Bst4CI ACNGT 5 cut(s) 226, 232, 286, 373, 452
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 426, 511
BstBAI YACGTR 2 cut(s) 11, 517
BstDEI CTNAG 3 cut(s) 52, 468, 588
BstENI CCTNNNNNAGG 1 cut(s) 299
BstFNI CGCG 4 cut(s) 248, 270, 272, 411
BstH2I RGCGCY 2 cut(s) 514, 552
BstHHI GCGC 5 cut(s) 250, 272, 413, 513, 551
BstKTI GATC 1 cut(s) 327
BstMBI GATC 1 cut(s) 324
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 3 cut(s) 11, 385, 425
BstNI CCWGG 2 cut(s) 334, 569
BstSCI CCNGG 3 cut(s) 332, 506, 567
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 4 cut(s) 248, 270, 272, 411
BstV2I GAAGAC 3 cut(s) 162, 287, 599
BsuRI GGCC 2 cut(s) 215, 337
BtrI CACGTC 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 237, 291
CfoI GCGC 5 cut(s) 250, 272, 413, 513, 551
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 2 cut(s) 434, 529
Csp6I GTAC 3 cut(s) 340, 400, 407
CviAII CATG 2 cut(s) 174, 352
CviQI GTAC 3 cut(s) 340, 400, 407
DdeI CTNAG 3 cut(s) 52, 468, 588
DinI GGCGCC 1 cut(s) 512
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 464
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 529
Eco72I CACGTG 2 cut(s) 11, 517
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 332, 567
EgeI GGCGCC 1 cut(s) 512
EheI GGCGCC 1 cut(s) 512
FaeI CATG 2 cut(s) 177, 355
FaiI YATR 3 cut(s) 69, 175, 353
FaqI GGGAC 2 cut(s) 217, 477
FatI CATG 2 cut(s) 173, 351
FblI GTMKAC 1 cut(s) 264
FspBI CTAG 1 cut(s) 631
GlaI GCGC 5 cut(s) 249, 271, 412, 512, 550
GsaI CCCAGC 5 cut(s) 18, 153, 200, 204, 576
HaeII RGCGCY 2 cut(s) 514, 552
HaeIII GGCC 2 cut(s) 215, 337
HapII CCGG 2 cut(s) 251, 507
HgaI GACGC 2 cut(s) 434, 529
HhaI GCGC 5 cut(s) 250, 272, 413, 513, 551
Hin1I GRCGYC 2 cut(s) 426, 511
Hin1II CATG 2 cut(s) 177, 355
Hin6I GCGC 5 cut(s) 248, 270, 411, 511, 549
HinP1I GCGC 5 cut(s) 248, 270, 411, 511, 549
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 3 cut(s) 19, 209, 476
HpaII CCGG 2 cut(s) 251, 507
HphI GGTGA 2 cut(s) 265, 496
Hpy166II GTNNAC 3 cut(s) 265, 289, 369
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 3 cut(s) 265, 289, 369
Hpy99I CGWCG 3 cut(s) 332, 428, 434
HpyAV CCTTC 2 cut(s) 370, 466
HpyCH4III ACNGT 5 cut(s) 226, 232, 286, 373, 452
HpyCH4IV ACGT 4 cut(s) 10, 120, 384, 516
HpyCH4V TGCA 2 cut(s) 355, 496
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 385, 425
HpyF3I CTNAG 3 cut(s) 52, 468, 588
HpySE526I ACGT 4 cut(s) 10, 120, 384, 516
Hsp92I GRCGYC 2 cut(s) 426, 511
Hsp92II CATG 2 cut(s) 177, 355
HspAI GCGC 5 cut(s) 248, 270, 411, 511, 549
KasI GGCGCC 1 cut(s) 510
Kzo9I GATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 631
MaeII ACGT 4 cut(s) 10, 120, 384, 516
MaeIII GTNAC 6 cut(s) 106, 169, 226, 380, 517, 626
MalI GATC 1 cut(s) 326
MboI GATC 1 cut(s) 324
MboII GAAGA 5 cut(s) 70, 167, 292, 571, 604
MhlI GDGCHC 1 cut(s) 390
Mly113I GGCGCC 1 cut(s) 511
MlyI GAGTC 2 cut(s) 203, 470
MmeI TCCRAC 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 2 cut(s) 251, 507
MspR9I CCNGG 3 cut(s) 334, 508, 569
MvaI CCWGG 2 cut(s) 334, 569
MvnI CGCG 4 cut(s) 248, 270, 272, 411
MwoI GCNNNNNNNGC 3 cut(s) 11, 385, 425
NarI GGCGCC 1 cut(s) 511
NciI CCSGG 1 cut(s) 508
NdeII GATC 1 cut(s) 324
NlaIII CATG 2 cut(s) 177, 355
NlaIV GGNNCC 2 cut(s) 465, 512
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 3 cut(s) 226, 380, 517
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 429
PfeI GAWTC 1 cut(s) 19
Pfl23II CGTACG 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 428
PleI GAGTC 2 cut(s) 203, 470
PluTI GGCGCC 1 cut(s) 514
PmaCI CACGTG 2 cut(s) 11, 517
PmlI CACGTG 2 cut(s) 11, 517
PpsI GAGTC 2 cut(s) 203, 470
Ppu21I YACGTR 2 cut(s) 11, 517
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 332, 567
PspCI CACGTG 2 cut(s) 11, 517
PspFI CCCAGC 5 cut(s) 14, 149, 196, 200, 572
PspGI CCWGG 2 cut(s) 332, 567
PspLI CGTACG 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 465, 512
PspPI GGNCC 1 cut(s) 464
PspPPI RGGWCCY 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 3 cut(s) 341, 401, 408
RsaNI GTAC 3 cut(s) 340, 400, 407
SalI GTCGAC 1 cut(s) 263
Sau3AI GATC 1 cut(s) 324
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 2 cut(s) 203, 470
ScrFI CCNGG 3 cut(s) 334, 508, 569
SduI GDGCHC 1 cut(s) 390
SfoI GGCGCC 1 cut(s) 512
SgrAI CRCCGGYG 1 cut(s) 250
SinI GGWCC 1 cut(s) 464
SsiI CCGC 3 cut(s) 268, 393, 440
SspDI GGCGCC 1 cut(s) 510
SspMI CTAG 1 cut(s) 631
StyD4I CCNGG 3 cut(s) 332, 506, 567
TaaI ACNGT 5 cut(s) 226, 232, 286, 373, 452
TaiI ACGT 4 cut(s) 13, 123, 387, 519
TaqI TCGA 4 cut(s) 264, 363, 375, 432
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 1 cut(s) 19
TscAI CASTG 2 cut(s) 237, 291
TseFI GTSAC 3 cut(s) 226, 380, 517
Tsp45I GTSAC 3 cut(s) 226, 380, 517
TspDTI ATGAA 1 cut(s) 571
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 2 cut(s) 237, 291
Tth111I GACNNNGTC 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 464
XagI CCTNNNNNAGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 1 cut(s) 631
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.