Rh7CG129100

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0004983
Physical Location & Seq
Reverse (-)
10186097 .. 10186378
282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG129100.1

Sequence Viewer

Length: 282 bp
ATGCAGACCTTCGAGTGGACAGTCGAGCGTGACGTGCCCACCGCCACGTACTTCGTACGCGCCTACGCCTACGACGCCGCCGAGGTGGCGGTGGCTTACGGTCAAAACACGGGACCTAAGAAGGACTCCAACTTGTTTGAGGTGCAGTCTATCACCGGGCGCCACGTGACGCTTGATATTGTTTCCGTCTGCTTCAGCGCTTTCTCGGTTTTAGCCCTGGCTGGGTTCTTCATTGCTGAGAAGACGAGGGCAAAGTCGTCCAAAAGAAGTGTTGGTAACTAG

Protein Analysis

93

Amino Acids

10.23

Weight (kDa)

7.87

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 2 - 53 4.9e-19 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 159
AccII CGCG 1 cut(s) 60
AciI CCGC 3 cut(s) 42, 78, 89
AcuI CTGAAG 1 cut(s) 178
AcvI CACGTG 1 cut(s) 166
AcyI GRCGYC 2 cut(s) 75, 160
AfaI GTAC 2 cut(s) 50, 57
AfeI AGCGCT 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 15, 222
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 199
AspLEI GCGC 3 cut(s) 62, 162, 200
AspS9I GGNCC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 145
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 39
BanI GGYRCC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 166
BbsI GAAGAC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 1 cut(s) 157
BfaI CTAG 1 cut(s) 280
BfoI RGCGCY 2 cut(s) 163, 201
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 157, 218
Bme18I GGWCC 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 2 cut(s) 114, 161
BmrFI CCNGG 2 cut(s) 157, 218
BpiI GAAGAC 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 157
BsaAI YACGTR 2 cut(s) 48, 166
BsaHI GRCGYC 2 cut(s) 75, 160
BsaJI CCNNGG 2 cut(s) 81, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 222
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 81, 216
BseLI CCNNNNNNNGG 2 cut(s) 15, 222
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 228
BseSI GKGCMC 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 221
BsgI GTGCAG 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 60
BshNI GGYRCC 1 cut(s) 159
BsiSI CCGG 1 cut(s) 156
BsiWI CGTACG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 15, 222
BsmFI GGGAC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 39
BspACI CCGC 3 cut(s) 42, 78, 89
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 114, 161
BspT107I GGYRCC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 81, 216
BssNI GRCGYC 2 cut(s) 75, 160
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 22, 101
BstACI GRCGYC 2 cut(s) 75, 160
BstBAI YACGTR 2 cut(s) 48, 166
BstDEI CTNAG 2 cut(s) 117, 237
BstFNI CGCG 1 cut(s) 60
BstH2I RGCGCY 2 cut(s) 163, 201
BstHHI GCGC 3 cut(s) 62, 162, 200
BstMWI GCNNNNNNNGC 3 cut(s) 34, 74, 86
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 155, 216
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 1 cut(s) 60
BstV2I GAAGAC 1 cut(s) 248
BtrI CACGTC 1 cut(s) 34
CfoI GCGC 3 cut(s) 62, 162, 200
Cfr13I GGNCC 1 cut(s) 113
CseI GACGC 2 cut(s) 83, 178
Csp6I GTAC 2 cut(s) 49, 56
CviJI RGCY 3 cut(s) 95, 215, 221
CviKI_1 RGCY 3 cut(s) 95, 215, 221
CviQI GTAC 2 cut(s) 49, 56
DdeI CTNAG 2 cut(s) 117, 237
DinI GGCGCC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 113
Eco47III AGCGCT 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 178
Eco72I CACGTG 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 216
EgeI GGCGCC 1 cut(s) 161
EheI GGCGCC 1 cut(s) 161
FaqI GGGAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 280
GlaI GCGC 3 cut(s) 61, 161, 199
GluI GCNGC 1 cut(s) 78
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 2 cut(s) 163, 201
HapII CCGG 1 cut(s) 156
HgaI GACGC 2 cut(s) 83, 178
HhaI GCGC 3 cut(s) 62, 162, 200
Hin1I GRCGYC 2 cut(s) 75, 160
Hin6I GCGC 3 cut(s) 60, 160, 198
HinP1I GCGC 3 cut(s) 60, 160, 198
HinfI GANTC 1 cut(s) 125
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 18
Hpy8I GTNNAC 1 cut(s) 18
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 19, 115
HpyCH4III ACNGT 2 cut(s) 22, 101
HpyCH4IV ACGT 3 cut(s) 33, 47, 165
HpyCH4V TGCA 2 cut(s) 4, 145
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 74, 86
HpyF3I CTNAG 2 cut(s) 117, 237
HpySE526I ACGT 3 cut(s) 33, 47, 165
Hsp92I GRCGYC 2 cut(s) 75, 160
HspAI GCGC 3 cut(s) 60, 160, 198
KasI GGCGCC 1 cut(s) 159
LpnPI CCDG 4 cut(s) 169, 203, 207, 230
MaeI CTAG 1 cut(s) 280
MaeII ACGT 3 cut(s) 33, 47, 165
MaeIII GTNAC 3 cut(s) 29, 166, 275
MboII GAAGA 2 cut(s) 220, 253
MhlI GDGCHC 1 cut(s) 39
Mly113I GGCGCC 1 cut(s) 160
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 3 cut(s) 76, 133, 240
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 157, 218
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 34, 74, 86
NarI GGCGCC 1 cut(s) 160
NciI CCSGG 1 cut(s) 157
NlaIV GGNNCC 2 cut(s) 114, 161
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 2 cut(s) 29, 166
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 78
Pfl23II CGTACG 1 cut(s) 55
PkrI GCNGC 1 cut(s) 79
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 163
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 1 cut(s) 119
Ppu21I YACGTR 2 cut(s) 48, 166
PpuMI RGGWCCY 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 216
PspCI CACGTG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 216
PspLI CGTACG 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 114, 161
PspPI GGNCC 1 cut(s) 113
PspPPI RGGWCCY 1 cut(s) 113
RsaI GTAC 2 cut(s) 50, 57
RsaNI GTAC 2 cut(s) 49, 56
SatI GCNGC 1 cut(s) 78
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 157, 218
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 11, 36, 50, 87, 118, 144, 168
SfoI GGCGCC 1 cut(s) 161
SinI GGWCC 1 cut(s) 113
SsiI CCGC 3 cut(s) 42, 78, 89
SspDI GGCGCC 1 cut(s) 159
SspMI CTAG 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 155, 216
TaaI ACNGT 2 cut(s) 22, 101
TaiI ACGT 3 cut(s) 36, 50, 168
TaqI TCGA 2 cut(s) 12, 24
TauI GCSGC 1 cut(s) 80
TseFI GTSAC 2 cut(s) 29, 166
Tsp45I GTSAC 2 cut(s) 29, 166
TspDTI ATGAA 1 cut(s) 220
TspGWI ACGGA 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 113
XspI CTAG 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.