MD14G1170300.v1.1

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004983
Physical Location & Seq
Reverse (-)
26467313 .. 26468995
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1170300.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCATCTCATGGGATTCTGTTGGCTTCACTTCTTGTGCTCTCTTGCTTATCCCAAACCTCCTATGGGATTGTCCTCTTTTCTTCTCTCAAGGATAGTCTCTTTGTTACTGCTTCACACGCAGAAAACCAGGTTCTGAAAGCAGGGATAGACAAGCTCACCGTTTCATGGGGGCTAAACCAGAGCTCCGAAGCCGGCACCGACTCAACCTTCAAGACCATCAAGGTCAAGTTATGCTACGCGCCGGTGAGCCAAGTCGACCGCGGGTGGAGGAAGACCGTCGACAACCTCGCCAAGGACAAGACATGCCTGTTGAAGATCGTCGAAAGGTCGTACACCAGCAAGTCCAACGACAGCGTCGAGTGGACCATCGAGCGCGACGTGCCGGGCGCCACGTACTTCGTACGCGCCTACGCCTTCAACGCCGCGGGTCAGGAGGTGGCGTATGGTCAGACTACGAACGCCAAAAAGACCGCCAACTTGTTTGTGGTGGAGGGAATCAGCGGCCGCCACATGTCGCTTGACATTGCATCGGTTGTCTTCAGTGTTTTCTCGGTTTTGTCCCTGGGTGGTTTCTTTATTGCTGAGAAGAGAAGGGCAAAGTCGTCATAA

Protein Analysis

204

Amino Acids

22.05

Weight (kDa)

9.24

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 27 - 169 2.6e-61 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 197, 389
AccI GTMKAC 2 cut(s) 258, 282
AccII CGCG 5 cut(s) 242, 264, 378, 408, 428
AciI CCGC 7 cut(s) 262, 264, 426, 428, 474, 504, 508
AcoI YGGCCR 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 3 cut(s) 335, 398, 405
AfiI CCNNNNNNNGG 3 cut(s) 66, 168, 295
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 214, 316, 421
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 2 cut(s) 129, 564
AluBI AGCT 2 cut(s) 157, 186
AluI AGCT 2 cut(s) 157, 186
Alw21I GWGCWC 2 cut(s) 42, 188
Alw26I GTCTC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 136
AoxI GGCC 1 cut(s) 505
AspLEI GCGC 4 cut(s) 244, 378, 392, 410
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 151, 259
AvaII GGWCC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 197, 389
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 2 cut(s) 281, 532
Bbv12I GWGCWC 2 cut(s) 42, 188
BccI CCATC 2 cut(s) 227, 377
BciT130I CCWGG 2 cut(s) 131, 566
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 393
BisI GCNGC 3 cut(s) 426, 505, 508
BlsI GCNGC 3 cut(s) 427, 506, 509
Bme1390I CCNGG 3 cut(s) 131, 387, 566
Bme18I GGWCC 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 2 cut(s) 199, 391
BmrFI CCNGG 3 cut(s) 131, 387, 566
BmsI GCATC 2 cut(s) 14, 539
BpiI GAAGAC 2 cut(s) 281, 532
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 5 cut(s) 262, 294, 426, 564, 565
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 168, 295
Bse118I RCCGGY 2 cut(s) 194, 244
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 131, 566
BseDI CCNNGG 5 cut(s) 262, 294, 426, 564, 565
BseLI CCNNNNNNNGG 3 cut(s) 66, 168, 295
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 576
BseX3I CGGCCG 1 cut(s) 505
Bsh1236I CGCG 5 cut(s) 242, 264, 378, 408, 428
Bsh1285I CGRYCG 2 cut(s) 262, 508
BshFI GGCC 1 cut(s) 507
BshNI GGYRCC 2 cut(s) 197, 389
BsiEI CGRYCG 2 cut(s) 262, 508
BsiHKAI GWGCWC 2 cut(s) 42, 188
BsiSI CCGG 3 cut(s) 195, 245, 386
BsiWI CGTACG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 3 cut(s) 66, 168, 295
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 507
Bsp1286I GDGCHC 2 cut(s) 42, 188
Bsp143I GATC 1 cut(s) 318
BspACI CCGC 7 cut(s) 262, 264, 426, 428, 474, 504, 508
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 5 cut(s) 242, 264, 378, 408, 428
BspLI GGNNCC 2 cut(s) 199, 391
BspT107I GGYRCC 2 cut(s) 197, 389
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 2 cut(s) 194, 244
BssAI RCCGGY 2 cut(s) 194, 244
BssECI CCNNGG 5 cut(s) 262, 294, 426, 564, 565
BssMI GATC 1 cut(s) 318
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 131, 566
Bst4CI ACNGT 2 cut(s) 163, 280
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 585
BstDSI CCRYGG 2 cut(s) 262, 426
BstENI CCTNNNNNAGG 1 cut(s) 293
BstFNI CGCG 5 cut(s) 242, 264, 378, 408, 428
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 4 cut(s) 244, 378, 392, 410
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 318
BstMCI CGRYCG 2 cut(s) 262, 508
BstMWI GCNNNNNNNGC 3 cut(s) 119, 382, 422
BstNI CCWGG 2 cut(s) 131, 566
BstNSI RCATGY 2 cut(s) 309, 517
BstSCI CCNGG 3 cut(s) 129, 385, 564
BstUI CGCG 5 cut(s) 242, 264, 378, 408, 428
BstV2I GAAGAC 2 cut(s) 281, 532
BstZI CGGCCG 1 cut(s) 505
BsuRI GGCC 1 cut(s) 507
BtgI CCRYGG 2 cut(s) 262, 426
BtrI CACGTC 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 136
CciNI GCGGCCGC 1 cut(s) 505
CfoI GCGC 4 cut(s) 244, 378, 392, 410
Cfr10I RCCGGY 2 cut(s) 194, 244
Cfr13I GGNCC 1 cut(s) 366
Cfr42I CCGCGG 2 cut(s) 265, 429
CseI GACGC 1 cut(s) 346
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 3 cut(s) 334, 397, 404
CviAII CATG 4 cut(s) 11, 168, 306, 514
CviJI RGCY 7 cut(s) 26, 157, 175, 186, 194, 252, 507
CviKI_1 RGCY 7 cut(s) 26, 157, 175, 186, 194, 252, 507
CviQI GTAC 3 cut(s) 334, 397, 404
DdeI CTNAG 1 cut(s) 585
DinI GGCGCC 1 cut(s) 391
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 505
EagI CGGCCG 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 186
EclXI CGGCCG 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 366
Eco52I CGGCCG 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 526
EcoICRI GAGCTC 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 129, 564
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 188
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 4 cut(s) 14, 171, 309, 517
FaiI YATR 8 cut(s) 12, 66, 169, 235, 307, 447, 515, 610
FaqI GGGAC 1 cut(s) 547
FatI CATG 4 cut(s) 10, 167, 305, 513
FauI CCCGC 2 cut(s) 257, 421
FblI GTMKAC 2 cut(s) 258, 282
Fnu4HI GCNGC 3 cut(s) 426, 505, 508
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 3 cut(s) 426, 505, 508
GlaI GCGC 4 cut(s) 243, 377, 391, 409
GluI GCNGC 3 cut(s) 426, 505, 508
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 1 cut(s) 507
HapII CCGG 3 cut(s) 195, 245, 386
HgaI GACGC 1 cut(s) 346
HhaI GCGC 4 cut(s) 244, 378, 392, 410
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 4 cut(s) 14, 171, 309, 517
Hin6I GCGC 4 cut(s) 242, 376, 390, 408
HinP1I GCGC 4 cut(s) 242, 376, 390, 408
HincII GTYRAC 2 cut(s) 259, 283
HindII GTYRAC 2 cut(s) 259, 283
HinfI GANTC 3 cut(s) 16, 203, 498
HpaII CCGG 3 cut(s) 195, 245, 386
HphI GGTGA 2 cut(s) 151, 259
Hpy166II GTNNAC 4 cut(s) 259, 283, 336, 366
Hpy188I TCNGA 3 cut(s) 138, 190, 453
Hpy188III TCNNGA 2 cut(s) 214, 434
Hpy8I GTNNAC 4 cut(s) 259, 283, 336, 366
Hpy99I CGWCG 4 cut(s) 284, 326, 362, 383
HpyAV CCTTC 3 cut(s) 220, 427, 588
HpyCH4III ACNGT 2 cut(s) 163, 280
HpyCH4IV ACGT 2 cut(s) 381, 395
HpyCH4V TGCA 1 cut(s) 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 382, 422
HpyF3I CTNAG 1 cut(s) 585
HpySE526I ACGT 2 cut(s) 381, 395
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 4 cut(s) 14, 171, 309, 517
HspAI GCGC 4 cut(s) 242, 376, 390, 408
KasI GGCGCC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
KspI CCGCGG 2 cut(s) 265, 429
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 191
LweI GCATC 2 cut(s) 14, 539
MabI ACCWGGT 1 cut(s) 129
MaeII ACGT 2 cut(s) 381, 395
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 5 cut(s) 75, 286, 328, 532, 601
MhlI GDGCHC 2 cut(s) 42, 188
Mly113I GGCGCC 1 cut(s) 390
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 70, 86, 264, 299, 430, 487
MroNI GCCGGC 1 cut(s) 194
MspA1I CMGCKG 3 cut(s) 264, 428, 504
MspI CCGG 3 cut(s) 195, 245, 386
MspR9I CCNGG 3 cut(s) 131, 387, 566
MvaI CCWGG 2 cut(s) 131, 566
MvnI CGCG 5 cut(s) 242, 264, 378, 408, 428
MwoI GCNNNNNNNGC 3 cut(s) 119, 382, 422
NaeI GCCGGC 1 cut(s) 196
NarI GGCGCC 1 cut(s) 390
NciI CCSGG 1 cut(s) 387
NdeII GATC 1 cut(s) 318
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 4 cut(s) 14, 171, 309, 517
NlaIV GGNNCC 2 cut(s) 199, 391
NotI GCGGCCGC 1 cut(s) 505
NspI RCATGY 2 cut(s) 309, 517
PasI CCCWGGG 1 cut(s) 565
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 378
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 16, 498
Pfl23II CGTACG 1 cut(s) 403
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 3 cut(s) 427, 506, 509
PleI GAGTC 1 cut(s) 197
PluTI GGCGCC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 197
Ppu21I YACGTR 1 cut(s) 396
PscI ACATGT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 129, 564
PspGI CCWGG 2 cut(s) 129, 564
PspLI CGTACG 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 199, 391
PspPI GGNCC 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 3 cut(s) 335, 398, 405
RsaNI GTAC 3 cut(s) 334, 397, 404
SacI GAGCTC 1 cut(s) 188
SacII CCGCGG 2 cut(s) 265, 429
SalI GTCGAC 2 cut(s) 257, 281
SatI GCNGC 3 cut(s) 426, 505, 508
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 3 cut(s) 131, 387, 566
SduI GDGCHC 2 cut(s) 42, 188
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 2 cut(s) 14, 539
SfoI GGCGCC 1 cut(s) 391
Sfr303I CCGCGG 2 cut(s) 265, 429
SgrAI CRCCGGYG 1 cut(s) 244
SgrBI CCGCGG 2 cut(s) 265, 429
SinI GGWCC 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SsiI CCGC 7 cut(s) 262, 264, 426, 428, 474, 504, 508
SspDI GGCGCC 1 cut(s) 389
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 129, 385, 564
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 163, 280
TaiI ACGT 2 cut(s) 384, 398
TaqI TCGA 5 cut(s) 258, 282, 324, 360, 372
TauI GCSGC 3 cut(s) 428, 507, 510
TfiI GAWTC 2 cut(s) 16, 498
TscAI CASTG 1 cut(s) 550
TspDTI ATGAA 1 cut(s) 156
TspRI CASTG 1 cut(s) 550
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 293
XceI RCATGY 2 cut(s) 309, 517
XcmI CCANNNNNNNNNTGG 2 cut(s) 62, 484
XmiI GTMKAC 2 cut(s) 258, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.