RLG00000004372

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004983
Physical Location & Seq
Forward (+)
58782049 .. 58783571
1523 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004372

Sequence Viewer

Length: 753 bp
ATGCCAGCGACCACGGCGGTGACAGAGAGAGGAGAGAGATCGAGAACGATTGGAGCAGCTAGTGATTCTGCAAAACGATCGGAGCAGAGGCCGAGAGCATCATTGACGAGGCCGGGAGCAACTCGACAATCTCCTCCAGAAACCTCTTATGCAAATGGCGTCGTCCTCTTTTCCTCTCTGCCCAACACTTTAGAAATGATCGTGTCACCCCACAAACCAGAAGAGCTTATGAAACCTGCGGAAGGTAGTTGGACTTTTGGTGGGACGTTAAAAGCTGGAGAAGACAAACTCAGCATCTCATGGCATCTAAACCAGAGTTTCCCAGCCGGAATCACCGACTCGGGGTACAAGACCGTCAAGGTGAAGCTGTGCTACGTGTCGGTGTGTCAAGTGGACCGCGGGTGGAGAAAGACGGTGGACAACCTCACAAAGGACAAGACGTGCCAGTTCAAGATCGAAACTAGACCATACAAACACGGCTCTAGCAAGTGGAAGTACATAGATTGGACCATAAATCGCGACATGCCTTCTGCTTCGTACTTCGTACGCTCCTACTCTTACGACGGTGCAGGTGTTGCGGTGGCTTACGGTCAAAGCACGGGACCGAAAAAAAACTACAACATATTTGAGGTCCAGGGGATCACCGAAAGACACAAGACGCTTGATGTGGTATCGATTTGCTTTAGTGCTTTCTCCATTTTGTCCTTGGTTGGGTTCTTTGTTGTTGAGAAGACAAGGGCCAAGCCTACCTAA

Protein Analysis

251

Amino Acids

27.74

Weight (kDa)

9.55

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 57 - 215 3.3e-49 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 560
Acc36I ACCTGC 2 cut(s) 244, 560
AccII CGCG 2 cut(s) 399, 519
AciI CCGC 5 cut(s) 17, 239, 397, 399, 578
AclWI GGATC 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 4 cut(s) 347, 497, 539, 546
AfiI CCNNNNNNNGG 4 cut(s) 242, 342, 430, 711
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 1 cut(s) 451
AjiI CACGTC 1 cut(s) 441
AjnI CCWGG 1 cut(s) 633
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 4 cut(s) 59, 226, 275, 367
AluI AGCT 4 cut(s) 59, 226, 275, 367
AlwI GGATC 1 cut(s) 647
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 3 cut(s) 89, 110, 738
ApeKI GCWGC 1 cut(s) 56
AspS9I GGNCC 5 cut(s) 394, 507, 602, 631, 738
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 5 cut(s) 31, 198, 325, 373, 634
AvaI CYCGRG 1 cut(s) 340
AvaII GGWCC 4 cut(s) 394, 507, 602, 631
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
BbsI GAAGAC 2 cut(s) 288, 737
BbvI GCAGC 1 cut(s) 68
BceAI ACGGC 2 cut(s) 30, 493
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 1 cut(s) 635
BcnI CCSGG 1 cut(s) 114
BfaI CTAG 3 cut(s) 60, 462, 483
BfuAI ACCTGC 2 cut(s) 244, 560
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 114, 635
Bme18I GGWCC 4 cut(s) 394, 507, 602, 631
BmeT110I CYCGRG 1 cut(s) 340
BmgBI CACGTC 1 cut(s) 441
BmgT120I GGNCC 5 cut(s) 394, 507, 602, 631, 738
BmiI GGNNCC 1 cut(s) 603
BmrFI CCNGG 2 cut(s) 114, 635
BmsI GCATC 3 cut(s) 107, 303, 313
BpiI GAAGAC 2 cut(s) 288, 737
BpmI CTGGAG 2 cut(s) 120, 297
BpuMI CCSGG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 674
BsaAI YACGTR 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 4 cut(s) 12, 397, 634, 705
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 342, 430, 711
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 635
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 4 cut(s) 12, 397, 634, 705
BseLI CCNNNNNNNGG 4 cut(s) 242, 342, 430, 711
BseMII CTCAG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 2 cut(s) 45, 123
BseXI GCAGC 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 2 cut(s) 399, 519
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 3 cut(s) 91, 112, 740
BshVI ATCGAT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 340
BsiSI CCGG 2 cut(s) 113, 327
BsiWI CGTACG 1 cut(s) 544
BslFI GGGAC 2 cut(s) 277, 615
BslI CCNNNNNNNGG 4 cut(s) 242, 342, 430, 711
BsmFI GGGAC 2 cut(s) 277, 615
BsnI GGCC 3 cut(s) 91, 112, 740
BsoBI CYCGRG 1 cut(s) 340
Bsp143I GATC 5 cut(s) 38, 77, 198, 453, 639
Bsp68I TCGCGA 1 cut(s) 519
BspACI CCGC 5 cut(s) 17, 239, 397, 399, 578
BspANI GGCC 3 cut(s) 91, 112, 740
BspCNI CTCAG 1 cut(s) 303
BspDI ATCGAT 1 cut(s) 674
BspFNI CGCG 2 cut(s) 399, 519
BspLI GGNNCC 1 cut(s) 603
BspMI ACCTGC 2 cut(s) 244, 560
BspPI GGATC 1 cut(s) 647
BspQI GCTCTTC 1 cut(s) 216
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 4 cut(s) 12, 397, 634, 705
BssMI GATC 5 cut(s) 38, 77, 198, 453, 639
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 4 cut(s) 355, 415, 566, 590
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 290
BstDSI CCRYGG 2 cut(s) 12, 397
BstENI CCTNNNNNAGG 2 cut(s) 240, 428
BstFNI CGCG 2 cut(s) 399, 519
BstKTI GATC 5 cut(s) 41, 80, 201, 456, 642
BstMBI GATC 5 cut(s) 38, 77, 198, 453, 639
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 2 cut(s) 14, 575
BstNI CCWGG 1 cut(s) 635
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 2 cut(s) 112, 633
BstUI CGCG 2 cut(s) 399, 519
BstV1I GCAGC 1 cut(s) 68
BstV2I GAAGAC 2 cut(s) 288, 737
Bsu15I ATCGAT 1 cut(s) 674
BsuRI GGCC 3 cut(s) 91, 112, 740
BsuTUI ATCGAT 1 cut(s) 674
BtgI CCRYGG 2 cut(s) 12, 397
BtrI CACGTC 1 cut(s) 441
BtuMI TCGCGA 1 cut(s) 519
BveI ACCTGC 2 cut(s) 244, 560
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 5 cut(s) 394, 507, 602, 631, 738
Cfr42I CCGCGG 1 cut(s) 400
ClaI ATCGAT 1 cut(s) 674
CseI GACGC 2 cut(s) 148, 667
Csp6I GTAC 4 cut(s) 346, 496, 538, 545
CviAII CATG 2 cut(s) 300, 523
CviQI GTAC 4 cut(s) 346, 496, 538, 545
DdeI CTNAG 1 cut(s) 290
DpnI GATC 5 cut(s) 40, 79, 200, 455, 641
DpnII GATC 5 cut(s) 38, 77, 198, 453, 639
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 705
Eco47I GGWCC 4 cut(s) 394, 507, 602, 631
Eco88I CYCGRG 1 cut(s) 340
EcoNI CCTNNNNNAGG 2 cut(s) 240, 428
EcoRII CCWGG 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 2 cut(s) 303, 526
FaiI YATR 8 cut(s) 150, 230, 301, 469, 500, 512, 524, 623
FaqI GGGAC 2 cut(s) 277, 615
FatI CATG 2 cut(s) 299, 522
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 3 cut(s) 60, 462, 483
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 2 cut(s) 120, 297
HaeIII GGCC 3 cut(s) 91, 112, 740
HapII CCGG 2 cut(s) 113, 327
HgaI GACGC 2 cut(s) 148, 667
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 2 cut(s) 303, 526
HinfI GANTC 3 cut(s) 65, 330, 338
HpaII CCGG 2 cut(s) 113, 327
HphI GGTGA 5 cut(s) 31, 198, 325, 373, 634
Hpy166II GTNNAC 2 cut(s) 394, 418
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 4 cut(s) 42, 137, 451, 518
Hpy8I GTNNAC 2 cut(s) 394, 418
Hpy99I CGWCG 2 cut(s) 164, 566
HpyAV CCTTC 2 cut(s) 236, 537
HpyCH4III ACNGT 4 cut(s) 355, 415, 566, 590
HpyCH4IV ACGT 3 cut(s) 266, 375, 440
HpyCH4V TGCA 3 cut(s) 71, 152, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 575
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 3 cut(s) 266, 375, 440
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 2 cut(s) 303, 526
KspI CCGCGG 1 cut(s) 400
Kzo9I GATC 5 cut(s) 38, 77, 198, 453, 639
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 4 cut(s) 53, 82, 116, 554
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 3 cut(s) 107, 303, 313
MaeI CTAG 3 cut(s) 60, 462, 483
MaeII ACGT 3 cut(s) 266, 375, 440
MaeIII GTNAC 2 cut(s) 19, 204
MalI GATC 5 cut(s) 40, 79, 200, 455, 641
MboI GATC 5 cut(s) 38, 77, 198, 453, 639
MboII GAAGA 3 cut(s) 233, 293, 742
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 9 cut(s) 23, 81, 102, 144, 154, 176, 184, 434, 622
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 2 cut(s) 113, 327
MspR9I CCNGG 2 cut(s) 114, 635
MvaI CCWGG 1 cut(s) 635
MvnI CGCG 2 cut(s) 399, 519
MwoI GCNNNNNNNGC 2 cut(s) 14, 575
NciI CCSGG 1 cut(s) 114
NdeII GATC 5 cut(s) 38, 77, 198, 453, 639
NlaIII CATG 2 cut(s) 303, 526
NlaIV GGNNCC 1 cut(s) 603
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 19, 204
NruI TCGCGA 1 cut(s) 519
NspI RCATGY 1 cut(s) 526
OliI CACNNNNGTG 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 560
PciSI GCTCTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 65, 330
Pfl23II CGTACG 1 cut(s) 544
PkrI GCNGC 1 cut(s) 58
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 633
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 633
PspLI CGTACG 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 603
PspPI GGNCC 5 cut(s) 394, 507, 602, 631, 738
PvuI CGATCG 1 cut(s) 80
RruI TCGCGA 1 cut(s) 519
RsaI GTAC 4 cut(s) 347, 497, 539, 546
RsaNI GTAC 4 cut(s) 346, 496, 538, 545
RseI CAYNNNNRTG 1 cut(s) 17
SacII CCGCGG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 5 cut(s) 38, 77, 198, 453, 639
Sau96I GGNCC 5 cut(s) 394, 507, 602, 631, 738
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 114, 635
SfaNI GCATC 3 cut(s) 107, 303, 313
Sfr303I CCGCGG 1 cut(s) 400
SgrBI CCGCGG 1 cut(s) 400
SinI GGWCC 4 cut(s) 394, 507, 602, 631
SmiMI CAYNNNNRTG 1 cut(s) 17
SsiI CCGC 5 cut(s) 17, 239, 397, 399, 578
SspMI CTAG 3 cut(s) 60, 462, 483
StyD4I CCNGG 2 cut(s) 112, 633
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 4 cut(s) 355, 415, 566, 590
TaiI ACGT 3 cut(s) 269, 378, 443
TaqI TCGA 4 cut(s) 41, 124, 456, 674
TaqII GACCGA 1 cut(s) 619
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 2 cut(s) 65, 330
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TseFI GTSAC 2 cut(s) 19, 204
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 2 cut(s) 19, 204
TspDTI ATGAA 1 cut(s) 245
VpaK11BI GGWCC 4 cut(s) 394, 507, 602, 631
XagI CCTNNNNNAGG 2 cut(s) 240, 428
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XspI CTAG 3 cut(s) 60, 462, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.