FvH4_5g06392

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004983
Physical Location & Seq
Forward (+)
3753772 .. 3755292
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06392.t1

Sequence Viewer

Length: 633 bp
ATGGCTTCTCGTGTTGGGATTCTATTGGCTTCTGTGCTTGTCCTCTCCTGCTTGGCAGGAACCTCCTATGGTGTCCTCTTCTCCTCTCTGAAACAAACTCTTGTTGTAACTACTACACATGTTGCGGCAAACGGAACACTCCTGAAAGCTGGGGAAGACAAAATCTCAGTAACATGGGGATTGAACCAGAGCCTCCCAGCCGGGACTGACTCGGCCTACAAGACCGTCAAGGTGAAGCTCTGCTACGCGCCGGTGAGCCAAGTAGACCGCGGGTGGAGGAAGACAGTGGACAACCTCGTCAAGGACAAGACCTGTCAGAAGAAGATCGTCGCCAGGCCGTACAACGCCTCCACGGAGACCTTCGAGTGGACCATTGAGCGTGACGTGCCCACCGGCAAGTACTTCGTACGCACCTACGCCTTCGACGCCGAAGGTGTGGAGGTGGCTTACGGCCAAAACACGGGAGCTAAGAAAGACTCCAACTTGTTCGAGGTGCAGTCCATCACCGGCCGCCACGTGACGCTTGATATCGTCTCCGTCTGCTTCAGCGCTTTCTCTGTTTTGTCCCTGGCTGGGTTTTTCATTGCTGAGAAGACGAGGGCAAAGTCGTCCAAAAGAAATGTTGGCAACTAA

Protein Analysis

211

Amino Acids

22.77

Weight (kDa)

9.43

Isoelectric Point (pI)

22.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 27 - 170 5.1e-62 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 264
AccII CGCG 2 cut(s) 248, 270
AciI CCGC 4 cut(s) 125, 268, 270, 511
AcoI YGGCCR 2 cut(s) 451, 508
AcuI CTGAAG 1 cut(s) 529
AcvI CACGTG 1 cut(s) 517
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 3 cut(s) 341, 401, 408
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 4 cut(s) 301, 366, 460, 573
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 1 cut(s) 184
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 332, 567
AluBI AGCT 3 cut(s) 149, 238, 467
AluI AGCT 3 cut(s) 149, 238, 467
Alw26I GTCTC 2 cut(s) 350, 538
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 4 cut(s) 213, 335, 451, 508
AspLEI GCGC 2 cut(s) 250, 551
AspS9I GGNCC 1 cut(s) 369
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 244, 265, 496
AvaII GGWCC 1 cut(s) 369
BaeGI GKGCMC 1 cut(s) 390
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BauI CACGAG 1 cut(s) 9
BbrPI CACGTG 1 cut(s) 517
BbsI GAAGAC 3 cut(s) 162, 287, 599
BccI CCATC 1 cut(s) 509
BceAI ACGGC 2 cut(s) 322, 466
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 2 cut(s) 334, 569
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 350, 538
BfoI RGCGCY 1 cut(s) 552
BisI GCNGC 2 cut(s) 126, 511
BlsI GCNGC 2 cut(s) 127, 512
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 3 cut(s) 202, 334, 569
Bme18I GGWCC 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 3 cut(s) 202, 334, 569
BpiI GAAGAC 3 cut(s) 162, 287, 599
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 517
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 268, 351, 567
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 366, 460, 573
Bse118I RCCGGY 3 cut(s) 250, 392, 506
Bse3DI GCAATG 1 cut(s) 582
BseBI CCWGG 2 cut(s) 334, 569
BseDI CCNNGG 3 cut(s) 268, 351, 567
BseLI CCNNNNNNNGG 4 cut(s) 301, 366, 460, 573
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 180, 579
BseRI GAGGAG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 390
BseX3I CGGCCG 1 cut(s) 508
BseYI CCCAGC 3 cut(s) 149, 196, 572
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 2 cut(s) 248, 270
Bsh1285I CGRYCG 1 cut(s) 511
BshFI GGCC 4 cut(s) 215, 337, 453, 510
BsiEI CGRYCG 1 cut(s) 511
BsiSI CCGG 4 cut(s) 201, 251, 393, 507
BsiWI CGTACG 1 cut(s) 406
BslFI GGGAC 2 cut(s) 217, 550
BslI CCNNNNNNNGG 4 cut(s) 301, 366, 460, 573
BsmAI GTCTC 2 cut(s) 350, 538
BsmBI CGTCTC 1 cut(s) 538
BsmFI GGGAC 2 cut(s) 217, 550
BsnI GGCC 4 cut(s) 215, 337, 453, 510
Bso31I GGTCTC 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 1 cut(s) 324
BspACI CCGC 4 cut(s) 125, 268, 270, 511
BspANI GGCC 4 cut(s) 215, 337, 453, 510
BspCNI CTCAG 2 cut(s) 179, 580
BspFNI CGCG 2 cut(s) 248, 270
BspLI GGNNCC 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 3 cut(s) 250, 392, 506
BssAI RCCGGY 3 cut(s) 250, 392, 506
BssECI CCNNGG 3 cut(s) 268, 351, 567
BssMI GATC 1 cut(s) 324
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 9
Bst2UI CCWGG 2 cut(s) 334, 569
Bst4CI ACNGT 2 cut(s) 226, 286
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 517
BstDEI CTNAG 3 cut(s) 166, 468, 588
BstDSI CCRYGG 2 cut(s) 268, 351
BstENI CCTNNNNNAGG 1 cut(s) 299
BstFNI CGCG 2 cut(s) 248, 270
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 250, 551
BstKTI GATC 1 cut(s) 327
BstMAI GTCTC 2 cut(s) 350, 538
BstMBI GATC 1 cut(s) 324
BstMCI CGRYCG 1 cut(s) 511
BstMWI GCNNNNNNNGC 2 cut(s) 385, 425
BstNI CCWGG 2 cut(s) 334, 569
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 200, 332, 567
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 2 cut(s) 248, 270
BstV2I GAAGAC 3 cut(s) 162, 287, 599
BstZI CGGCCG 1 cut(s) 508
BsuRI GGCC 4 cut(s) 215, 337, 453, 510
BtgI CCRYGG 2 cut(s) 268, 351
BtrI CACGTC 1 cut(s) 385
BtsIMutI CAGTG 1 cut(s) 291
CfoI GCGC 2 cut(s) 250, 551
Cfr10I RCCGGY 3 cut(s) 250, 392, 506
Cfr13I GGNCC 1 cut(s) 369
Cfr42I CCGCGG 1 cut(s) 271
CseI GACGC 2 cut(s) 434, 529
Csp6I GTAC 3 cut(s) 340, 400, 407
CviAII CATG 2 cut(s) 119, 174
CviQI GTAC 3 cut(s) 340, 400, 407
DdeI CTNAG 3 cut(s) 166, 468, 588
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 2 cut(s) 451, 508
EagI CGGCCG 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EclXI CGGCCG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 350
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 369
Eco47III AGCGCT 1 cut(s) 550
Eco52I CGGCCG 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 529
Eco72I CACGTG 1 cut(s) 517
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 332, 567
EcoRV GATATC 1 cut(s) 529
Esp3I CGTCTC 1 cut(s) 538
FaeI CATG 2 cut(s) 122, 177
FaiI YATR 3 cut(s) 69, 120, 175
FaqI GGGAC 2 cut(s) 217, 550
FatI CATG 2 cut(s) 118, 173
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 126, 511
Fsp4HI GCNGC 2 cut(s) 126, 511
GlaI GCGC 2 cut(s) 249, 550
GluI GCNGC 2 cut(s) 126, 511
GsaI CCCAGC 3 cut(s) 153, 200, 576
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 4 cut(s) 215, 337, 453, 510
HapII CCGG 4 cut(s) 201, 251, 393, 507
HgaI GACGC 2 cut(s) 434, 529
HhaI GCGC 2 cut(s) 250, 551
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 2 cut(s) 122, 177
Hin6I GCGC 2 cut(s) 248, 549
HinP1I GCGC 2 cut(s) 248, 549
HinfI GANTC 3 cut(s) 19, 209, 476
HpaII CCGG 4 cut(s) 201, 251, 393, 507
HphI GGTGA 3 cut(s) 244, 265, 496
Hpy166II GTNNAC 3 cut(s) 265, 289, 369
Hpy188I TCNGA 2 cut(s) 90, 318
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 3 cut(s) 265, 289, 369
Hpy99I CGWCG 2 cut(s) 332, 428
HpyAV CCTTC 3 cut(s) 370, 425, 430
HpyCH4III ACNGT 2 cut(s) 226, 286
HpyCH4IV ACGT 2 cut(s) 384, 516
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 425
HpyF3I CTNAG 3 cut(s) 166, 468, 588
HpySE526I ACGT 2 cut(s) 384, 516
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 2 cut(s) 122, 177
HspAI GCGC 2 cut(s) 248, 549
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 1 cut(s) 324
LmnI GCTCC 1 cut(s) 464
MaeII ACGT 2 cut(s) 384, 516
MaeIII GTNAC 4 cut(s) 106, 169, 380, 517
MalI GATC 1 cut(s) 326
MboI GATC 1 cut(s) 324
MboII GAAGA 6 cut(s) 70, 167, 292, 331, 334, 604
MhlI GDGCHC 1 cut(s) 390
MlyI GAGTC 2 cut(s) 203, 470
MmeI TCCRAC 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 4 cut(s) 201, 251, 393, 507
MspR9I CCNGG 3 cut(s) 202, 334, 569
MvaI CCWGG 2 cut(s) 334, 569
MvnI CGCG 2 cut(s) 248, 270
MwoI GCNNNNNNNGC 2 cut(s) 385, 425
NciI CCSGG 1 cut(s) 202
NdeII GATC 1 cut(s) 324
NlaIII CATG 2 cut(s) 122, 177
NlaIV GGNNCC 1 cut(s) 61
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 380, 517
NspI RCATGY 1 cut(s) 122
PciI ACATGT 1 cut(s) 118
PfeI GAWTC 1 cut(s) 19
Pfl23II CGTACG 1 cut(s) 406
PkrI GCNGC 2 cut(s) 127, 512
PleI GAGTC 2 cut(s) 203, 470
PmaCI CACGTG 1 cut(s) 517
PmlI CACGTG 1 cut(s) 517
PpsI GAGTC 2 cut(s) 203, 470
Ppu21I YACGTR 1 cut(s) 517
PscI ACATGT 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 332, 567
PspCI CACGTG 1 cut(s) 517
PspFI CCCAGC 3 cut(s) 149, 196, 572
PspGI CCWGG 2 cut(s) 332, 567
PspLI CGTACG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 1 cut(s) 369
RsaI GTAC 3 cut(s) 341, 401, 408
RsaNI GTAC 3 cut(s) 340, 400, 407
SacII CCGCGG 1 cut(s) 271
SatI GCNGC 2 cut(s) 126, 511
Sau3AI GATC 1 cut(s) 324
Sau96I GGNCC 1 cut(s) 369
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 2 cut(s) 203, 470
ScrFI CCNGG 3 cut(s) 202, 334, 569
SduI GDGCHC 1 cut(s) 390
Sfr303I CCGCGG 1 cut(s) 271
SgrAI CRCCGGYG 1 cut(s) 250
SgrBI CCGCGG 1 cut(s) 271
SinI GGWCC 1 cut(s) 369
SsiI CCGC 4 cut(s) 125, 268, 270, 511
StyD4I CCNGG 3 cut(s) 200, 332, 567
TaaI ACNGT 2 cut(s) 226, 286
TaiI ACGT 2 cut(s) 387, 519
TaqI TCGA 3 cut(s) 363, 423, 489
TatI WGTACW 1 cut(s) 399
TauI GCSGC 2 cut(s) 128, 513
TfiI GAWTC 1 cut(s) 19
TscAI CASTG 1 cut(s) 291
TseFI GTSAC 2 cut(s) 380, 517
Tsp45I GTSAC 2 cut(s) 380, 517
TspDTI ATGAA 1 cut(s) 571
TspGWI ACGGA 3 cut(s) 147, 368, 526
TspRI CASTG 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 369
XagI CCTNNNNNAGG 1 cut(s) 299
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.