FvH4_5g06400

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004983
Physical Location & Seq
Forward (+)
3756012 .. 3757138
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06400.t1

Sequence Viewer

Length: 675 bp
ATGGCATCGCGTAGTGAGATTGTCTTCGCTTCCCTTCTTCTTTTTCTTTCGTGCATATCAGAACCCTCGTATGCAAATGGCGTCGTCCTCTTCTCCTCTCTGCCCAACACTATCGAAATGATCGTTTCACCCCACAAACCTGAACAGGTTGTGAAACCCGCCGAAGGTAGTTGGACCTTTGGTGGGATGTTAAAAGCTGGAGAAGACAAACTCAGCATCTCATGGCGTCTAAACCAGAGTTTCTCAGCCGGAACCACCGACTCAGCGTACAAGACTGTGAAGGTGAAGCTGTGCTACGCGCCGGTGAGTCAAGTGGACCGCGGGTGGAGGAAGACTGTGGACAACCTCGCCAAGGACAAGACATGCCAGTTCAAGATCGAAACTAGGGCATACAAACATGGCTCTAGCAAGTGGAAGTACGTAGATTGGACCATAAAGCGTGACGTGCCTACTGCCTCGTACTTCGTACGCGCCTACGCGTACGACGGCGCAGGTGTTGCGGTGGCTTACGGCCAAAGCACGGGACCTAAGAAGAACTACAACATATTTGAGGTTCAGGGGATCACCGGACGACACAAGACGCTTGATGTGGTGTCGATTTGCTTCAGTGCTTTCTCCGTTTTGTCCTTGGTTGGCTTCTTTGTTATTGAAAAGACCAGGGCAAAGCCGACCTAA

Protein Analysis

225

Amino Acids

24.81

Weight (kDa)

9.48

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 31 - 190 1.2e-53 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 482
AccII CGCG 5 cut(s) 10, 299, 321, 471, 479
AciI CCGC 4 cut(s) 159, 319, 321, 500
AclWI GGATC 1 cut(s) 569
AcoI YGGCCR 1 cut(s) 511
AcuI CTGAAG 1 cut(s) 589
AcyI GRCGYC 2 cut(s) 81, 226
AfaI GTAC 5 cut(s) 269, 419, 461, 468, 482
AfiI CCNNNNNNNGG 4 cut(s) 164, 183, 352, 520
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 2 cut(s) 373, 650
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 656
AluBI AGCT 2 cut(s) 197, 289
AluI AGCT 2 cut(s) 197, 289
AlwI GGATC 1 cut(s) 569
AoxI GGCC 1 cut(s) 511
AspLEI GCGC 3 cut(s) 301, 473, 491
AspS9I GGNCC 4 cut(s) 174, 316, 429, 524
AsuHPI GGTGA 4 cut(s) 120, 295, 316, 556
AvaII GGWCC 4 cut(s) 174, 316, 429, 524
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbsI GAAGAC 3 cut(s) 16, 210, 338
BceAI ACGGC 2 cut(s) 502, 526
BciT130I CCWGG 1 cut(s) 658
BfaI CTAG 2 cut(s) 384, 405
BfuAI ACCTGC 1 cut(s) 482
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 4 cut(s) 174, 316, 429, 524
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 4 cut(s) 174, 316, 429, 524
BmiI GGNNCC 2 cut(s) 253, 525
BmrFI CCNGG 1 cut(s) 658
BmsI GCATC 2 cut(s) 14, 225
BpiI GAAGAC 3 cut(s) 16, 210, 338
BpmI CTGGAG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 421
BsaHI GRCGYC 2 cut(s) 81, 226
BsaJI CCNNGG 4 cut(s) 319, 351, 627, 657
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 183, 352, 520
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 4 cut(s) 319, 351, 627, 657
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 4 cut(s) 164, 183, 352, 520
BseMII CTCAG 3 cut(s) 226, 258, 276
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 1 cut(s) 85
Bsh1236I CGCG 5 cut(s) 10, 299, 321, 471, 479
BshFI GGCC 1 cut(s) 513
BsiSI CCGG 3 cut(s) 249, 302, 567
BsiWI CGTACG 2 cut(s) 466, 480
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 4 cut(s) 164, 183, 352, 520
BsmFI GGGAC 1 cut(s) 537
BsnI GGCC 1 cut(s) 513
Bsp143I GATC 3 cut(s) 120, 375, 561
BspACI CCGC 4 cut(s) 159, 319, 321, 500
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 3 cut(s) 225, 257, 275
BspFNI CGCG 5 cut(s) 10, 299, 321, 471, 479
BspLI GGNNCC 2 cut(s) 253, 525
BspMI ACCTGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 301
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 4 cut(s) 319, 351, 627, 657
BssMI GATC 3 cut(s) 120, 375, 561
BssNI GRCGYC 2 cut(s) 81, 226
BssT1I CCWWGG 2 cut(s) 351, 627
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 2 cut(s) 277, 337
Bst6I CTCTTC 1 cut(s) 95
BstACI GRCGYC 2 cut(s) 81, 226
BstAPI GCANNNNNTGC 1 cut(s) 497
BstBAI YACGTR 1 cut(s) 421
BstDEI CTNAG 4 cut(s) 212, 244, 262, 528
BstDSI CCRYGG 1 cut(s) 319
BstENI CCTNNNNNAGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 192
BstFNI CGCG 5 cut(s) 10, 299, 321, 471, 479
BstHHI GCGC 3 cut(s) 301, 473, 491
BstKTI GATC 3 cut(s) 123, 378, 564
BstMBI GATC 3 cut(s) 120, 375, 561
BstMWI GCNNNNNNNGC 2 cut(s) 445, 497
BstNI CCWGG 1 cut(s) 658
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 656
BstSNI TACGTA 1 cut(s) 421
BstUI CGCG 5 cut(s) 10, 299, 321, 471, 479
BstV2I GAAGAC 3 cut(s) 16, 210, 338
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 613
BveI ACCTGC 1 cut(s) 482
CfoI GCGC 3 cut(s) 301, 473, 491
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 4 cut(s) 174, 316, 429, 524
Cfr42I CCGCGG 1 cut(s) 322
CseI GACGC 3 cut(s) 70, 215, 589
Csp6I GTAC 5 cut(s) 268, 418, 460, 467, 481
CviAII CATG 3 cut(s) 222, 363, 398
CviJI RGCY 8 cut(s) 197, 248, 289, 402, 506, 513, 636, 667
CviKI_1 RGCY 8 cut(s) 197, 248, 289, 402, 506, 513, 636, 667
CviQI GTAC 5 cut(s) 268, 418, 460, 467, 481
DdeI CTNAG 4 cut(s) 212, 244, 262, 528
DpnI GATC 3 cut(s) 122, 377, 563
DpnII GATC 3 cut(s) 120, 375, 561
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 421
Eco130I CCWWGG 2 cut(s) 351, 627
Eco47I GGWCC 4 cut(s) 174, 316, 429, 524
Eco57I CTGAAG 1 cut(s) 589
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 656
EcoT14I CCWWGG 2 cut(s) 351, 627
ErhI CCWWGG 2 cut(s) 351, 627
FaeI CATG 3 cut(s) 225, 366, 401
FaiI YATR 8 cut(s) 56, 72, 223, 364, 391, 399, 434, 545
FaqI GGGAC 1 cut(s) 537
FatI CATG 3 cut(s) 221, 362, 397
FauI CCCGC 2 cut(s) 166, 314
FokI GGATG 1 cut(s) 199
FspBI CTAG 2 cut(s) 384, 405
GlaI GCGC 3 cut(s) 300, 472, 490
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 1 cut(s) 513
HapII CCGG 3 cut(s) 249, 302, 567
HgaI GACGC 3 cut(s) 70, 215, 589
HhaI GCGC 3 cut(s) 301, 473, 491
Hin1I GRCGYC 2 cut(s) 81, 226
Hin1II CATG 3 cut(s) 225, 366, 401
Hin6I GCGC 3 cut(s) 299, 471, 489
HinP1I GCGC 3 cut(s) 299, 471, 489
HinfI GANTC 2 cut(s) 260, 307
HpaII CCGG 3 cut(s) 249, 302, 567
HphI GGTGA 4 cut(s) 120, 295, 316, 556
Hpy166II GTNNAC 2 cut(s) 316, 340
Hpy188I TCNGA 1 cut(s) 61
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 2 cut(s) 316, 340
Hpy99I CGWCG 2 cut(s) 86, 488
HpyAV CCTTC 3 cut(s) 44, 158, 274
HpyCH4III ACNGT 2 cut(s) 277, 337
HpyCH4IV ACGT 2 cut(s) 420, 444
HpyCH4V TGCA 2 cut(s) 54, 74
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 497
HpyF3I CTNAG 4 cut(s) 212, 244, 262, 528
HpySE526I ACGT 2 cut(s) 420, 444
Hsp92I GRCGYC 2 cut(s) 81, 226
Hsp92II CATG 3 cut(s) 225, 366, 401
HspAI GCGC 3 cut(s) 299, 471, 489
KspI CCGCGG 1 cut(s) 322
Kzo9I GATC 3 cut(s) 120, 375, 561
LweI GCATC 2 cut(s) 14, 225
MaeI CTAG 2 cut(s) 384, 405
MaeII ACGT 2 cut(s) 420, 444
MaeIII GTNAC 1 cut(s) 440
MalI GATC 3 cut(s) 122, 377, 563
MboI GATC 3 cut(s) 120, 375, 561
MboII GAAGA 6 cut(s) 16, 29, 82, 215, 343, 544
MluI ACGCGT 1 cut(s) 477
MlyI GAGTC 2 cut(s) 254, 316
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 7 cut(s) 76, 98, 106, 321, 356, 466, 544
MseI TTAA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 249, 302, 567
MspR9I CCNGG 1 cut(s) 658
MvaI CCWGG 1 cut(s) 658
MvnI CGCG 5 cut(s) 10, 299, 321, 471, 479
MwoI GCNNNNNNNGC 2 cut(s) 445, 497
NdeII GATC 3 cut(s) 120, 375, 561
NlaIII CATG 3 cut(s) 225, 366, 401
NlaIV GGNNCC 2 cut(s) 253, 525
NmuCI GTSAC 1 cut(s) 440
NspI RCATGY 1 cut(s) 366
PaqCI CACCTGC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 120
Pfl23II CGTACG 2 cut(s) 466, 480
PleI GAGTC 2 cut(s) 254, 315
PpsI GAGTC 2 cut(s) 254, 315
Ppu21I YACGTR 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 656
PspGI CCWGG 1 cut(s) 656
PspLI CGTACG 2 cut(s) 466, 480
PspN4I GGNNCC 2 cut(s) 253, 525
PspPI GGNCC 4 cut(s) 174, 316, 429, 524
PspPPI RGGWCCY 1 cut(s) 524
RsaI GTAC 5 cut(s) 269, 419, 461, 468, 482
RsaNI GTAC 5 cut(s) 268, 418, 460, 467, 481
SacII CCGCGG 1 cut(s) 322
SaqAI TTAA 1 cut(s) 191
Sau3AI GATC 3 cut(s) 120, 375, 561
Sau96I GGNCC 4 cut(s) 174, 316, 429, 524
SchI GAGTC 2 cut(s) 254, 316
ScrFI CCNGG 1 cut(s) 658
SfaNI GCATC 2 cut(s) 14, 225
Sfr303I CCGCGG 1 cut(s) 322
SgrAI CRCCGGYG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 322
SinI GGWCC 4 cut(s) 174, 316, 429, 524
SnaBI TACGTA 1 cut(s) 421
SsiI CCGC 4 cut(s) 159, 319, 321, 500
SspMI CTAG 2 cut(s) 384, 405
StyD4I CCNGG 1 cut(s) 656
StyI CCWWGG 2 cut(s) 351, 627
TaaI ACNGT 2 cut(s) 277, 337
TaiI ACGT 2 cut(s) 423, 447
TaqI TCGA 3 cut(s) 114, 378, 596
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 1 cut(s) 613
VpaK11BI GGWCC 4 cut(s) 174, 316, 429, 524
XagI CCTNNNNNAGG 1 cut(s) 350
XceI RCATGY 1 cut(s) 366
XspI CTAG 2 cut(s) 384, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.