AT5G50200

Nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004983
Physical Location & Seq
Forward (+)
20436180 .. 20437815
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50200.2

Sequence Viewer

Length: 633 bp
ATGGCGATCCAGAAGATCCTCTTTGCTTCACTTCTCATATGCTCACTGATCCAATCCATCCACGGGGCGGAAAAAGTAAGACTCTTCAAAGAGCTGGACAAAGGTGCACTTGATGTCACCACTAAACCCAGCCGAGAAGGACCAGGTGTTGTTTTGGATGCCGGCAAGGATACGTTGAACATTACATGGACGCTAAGCTCGATTGGGTCTAAAAGAGAGGCTGAATTTAAGATCATCAAAGTTAAGCTATGCTACGCTCCACCTAGCCAAGTTGACCGACCATGGCGCAAAACCCATGACGAGCTCTTCAAAGACAAGACCTGCCCACACAAGATCATAGCCAAGCCTTATGACAAAACACTTCAATCAACTACTTGGACTCTTGAGCGTGACATCCCCACCGGAACCTACTTCGTTCGTGCCTACGCGGTTGATGCCATTGGCCATGAAGTTGCCTATGGACAGAGCACCGACGATGCCAAGAAAACCAATCTCTTCAGCGTTCAGGCTATCAGTGGCCGCCACGCGTCCCTAGATATTGCCTCCATCTGTTTCAGTGTCTTCTCCGTCGTGGCTCTTGTCGTCTTCTTTGTCAATGAGAAGAGGAAGGCCAAGATAGAGCAAAGCAAATGA

Protein Analysis

210

Amino Acids

23.4

Weight (kDa)

9.28

Isoelectric Point (pI)

25.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 29 - 173 2.4e-59 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 329
AccII CGCG 2 cut(s) 428, 527
AciI CCGC 3 cut(s) 68, 428, 520
AclWI GGATC 2 cut(s) 10, 43
AcoI YGGCCR 2 cut(s) 442, 517
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 481
AfiI CCNNNNNNNGG 2 cut(s) 63, 67
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 88, 178, 310, 365
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 4 cut(s) 94, 198, 247, 304
AluI AGCT 4 cut(s) 94, 198, 247, 304
Alw21I GWGCWC 3 cut(s) 109, 306, 470
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 2 cut(s) 10, 43
AoxI GGCC 3 cut(s) 442, 517, 609
ApaLI GTGCAC 1 cut(s) 105
ApoI RAATTY 1 cut(s) 224
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 444
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 2 cut(s) 553, 577
Bbv12I GWGCWC 3 cut(s) 109, 306, 470
BccI CCATC 2 cut(s) 65, 554
BciT130I CCWGG 1 cut(s) 144
BciVI GTATCC 1 cut(s) 163
BfaI CTAG 2 cut(s) 264, 533
BfuAI ACCTGC 1 cut(s) 329
BfuI GTATCC 1 cut(s) 163
BisI GCNGC 1 cut(s) 520
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 1 cut(s) 521
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 3 cut(s) 148, 424, 466
BpiI GAAGAC 2 cut(s) 553, 577
Bpu1102I GCTNAGC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 61, 281
BsaWI WCCGGW 1 cut(s) 401
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 67
Bse118I RCCGGY 1 cut(s) 161
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 61, 281
BseGI GGATG 3 cut(s) 57, 163, 393
BseLI CCNNNNNNNGG 2 cut(s) 63, 67
BseSI GKGCMC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 128
Bsh1236I CGCG 2 cut(s) 428, 527
BshFI GGCC 3 cut(s) 444, 519, 611
BsiHKAI GWGCWC 3 cut(s) 109, 306, 470
BsiSI CCGG 2 cut(s) 162, 402
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 63, 67
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 444, 519, 611
Bsp1286I GDGCHC 3 cut(s) 109, 306, 470
Bsp143I GATC 5 cut(s) 6, 15, 48, 231, 333
Bsp1720I GCTNAGC 1 cut(s) 194
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 3 cut(s) 68, 428, 520
BspANI GGCC 3 cut(s) 444, 519, 611
BspFNI CGCG 2 cut(s) 428, 527
BspLI GGNNCC 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 329
BspPI GGATC 2 cut(s) 10, 43
BspQI GCTCTTC 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 61, 281
BssMI GATC 5 cut(s) 6, 15, 48, 231, 333
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 144
Bst6I CTCTTC 4 cut(s) 89, 311, 500, 596
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 2 cut(s) 61, 281
BstF5I GGATG 3 cut(s) 57, 163, 393
BstFNI CGCG 2 cut(s) 428, 527
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 5 cut(s) 9, 18, 51, 234, 336
BstMBI GATC 5 cut(s) 6, 15, 48, 231, 333
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSLI GKGCMC 1 cut(s) 109
BstUI CGCG 2 cut(s) 428, 527
BstV2I GAAGAC 2 cut(s) 553, 577
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 3 cut(s) 444, 519, 611
BtgI CCRYGG 2 cut(s) 61, 281
BtsCI GGATG 3 cut(s) 57, 163, 393
BtsIMutI CAGTG 3 cut(s) 44, 520, 562
BveI ACCTGC 1 cut(s) 329
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 140
CseI GACGC 2 cut(s) 199, 516
CsiI ACCWGGT 1 cut(s) 142
CviAII CATG 4 cut(s) 186, 282, 296, 446
DdeI CTNAG 1 cut(s) 194
DpnI GATC 5 cut(s) 8, 17, 50, 233, 335
DpnII GATC 5 cut(s) 6, 15, 48, 231, 333
EaeI YGGCCR 2 cut(s) 442, 517
Eam1104I CTCTTC 4 cut(s) 89, 311, 500, 596
EarI CTCTTC 4 cut(s) 89, 311, 500, 596
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 140
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 4 cut(s) 189, 285, 299, 449
FalI AAGNNNNNCTT 3 cut(s) 37, 93, 125
FaqI GGGAC 1 cut(s) 514
FatI CATG 4 cut(s) 185, 281, 295, 445
FauNDI CATATG 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 520
FokI GGATG 3 cut(s) 44, 170, 380
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 1 cut(s) 520
FspBI CTAG 2 cut(s) 264, 533
GlaI GCGC 1 cut(s) 287
GluI GCNGC 1 cut(s) 520
GsaI CCCAGC 1 cut(s) 132
HaeIII GGCC 3 cut(s) 444, 519, 611
HapII CCGG 2 cut(s) 162, 402
HgaI GACGC 2 cut(s) 199, 516
HhaI GCGC 1 cut(s) 288
Hin1II CATG 4 cut(s) 189, 285, 299, 449
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 81, 379
HpaII CCGG 2 cut(s) 162, 402
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 107, 274
Hpy188III TCNNGA 2 cut(s) 10, 383
Hpy8I GTNNAC 2 cut(s) 107, 274
Hpy99I CGWCG 2 cut(s) 476, 572
HpyAV CCTTC 2 cut(s) 131, 601
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 4 cut(s) 189, 285, 299, 449
HspAI GCGC 1 cut(s) 286
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 5 cut(s) 6, 15, 48, 231, 333
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 9 cut(s) 23, 80, 129, 142, 156, 175, 334, 415, 491
LweI GCATC 3 cut(s) 148, 424, 466
MabI ACCWGGT 1 cut(s) 142
MaeI CTAG 2 cut(s) 264, 533
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 2 cut(s) 115, 389
MalI GATC 5 cut(s) 8, 17, 50, 233, 335
MboI GATC 5 cut(s) 6, 15, 48, 231, 333
MboII GAAGA 7 cut(s) 25, 76, 298, 487, 553, 577, 613
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 3 cut(s) 109, 306, 470
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 1 cut(s) 224
MluI ACGCGT 1 cut(s) 525
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 2 cut(s) 75, 373
MnlI CCTC 4 cut(s) 29, 211, 553, 597
Mox20I TGGCCA 1 cut(s) 444
MroNI GCCGGC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 444
MseI TTAA 2 cut(s) 228, 243
Msp20I TGGCCA 1 cut(s) 444
MspI CCGG 2 cut(s) 162, 402
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 2 cut(s) 428, 527
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 163
NcoI CCATGG 1 cut(s) 281
NdeI CATATG 1 cut(s) 38
NdeII GATC 5 cut(s) 6, 15, 48, 231, 333
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 4 cut(s) 189, 285, 299, 449
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 158
NmuCI GTSAC 2 cut(s) 115, 389
PciSI GCTCTTC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PdiI GCCGGC 1 cut(s) 163
PkrI GCNGC 1 cut(s) 521
PleI GAGTC 2 cut(s) 75, 373
PpsI GAGTC 2 cut(s) 75, 373
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 140
PsuI RGATCY 1 cut(s) 15
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 228, 243
SatI GCNGC 1 cut(s) 520
Sau3AI GATC 5 cut(s) 6, 15, 48, 231, 333
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 2 cut(s) 75, 373
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 3 cut(s) 109, 306, 470
SexAI ACCWGGT 1 cut(s) 142
SfaNI GCATC 3 cut(s) 148, 424, 466
SinI GGWCC 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 1 cut(s) 224
SsiI CCGC 3 cut(s) 68, 428, 520
SspMI CTAG 2 cut(s) 264, 533
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 281
TaiI ACGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 200
TaqII GACCGA 1 cut(s) 291
TasI AATT 1 cut(s) 224
TauI GCSGC 1 cut(s) 522
Tru1I TTAA 2 cut(s) 228, 243
Tru9I TTAA 2 cut(s) 228, 243
TscAI CASTG 3 cut(s) 51, 520, 562
TseFI GTSAC 2 cut(s) 115, 389
Tsp45I GTSAC 2 cut(s) 115, 389
TspDTI ATGAA 1 cut(s) 462
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 3 cut(s) 51, 520, 562
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 1 cut(s) 224
XspI CTAG 2 cut(s) 264, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.