Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

14297044 .. 14302287

Gene Structure 5244 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G29010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGCGAAGAAGATTGGAGTAACGATGGAAGTTGGGAACGATGGTGTTGCTGTCATCACCATTTCTAACCCTCCTGTTAACTCTTTAGCCAGTCCAATTATTTCTGGGTTGAAGGAGAAGTTTCGTGACGCCAATCAAAGGAATGATGTTAAGGCCATCGTGTTGATCGGGAACAATGGAAGATTTTCGGGTGGTTTTGATATCAATGTCTTTCAGCAAGTTCATAAGACTGGGGATTTATCGCTTATGCCTGAAGTATCTGTTGAGCTTGTGTGTAACCTGATGGAAGACTCCAGGAAGCCGGTTGTTGCTGCAGTTGAAGGATTAGCTCTTGGTGGTGGTTTGGAACTTGCAATGGCGTGTCATGCCAGAGTTGCTGCTCCGAAAGCTCAATTAGGCTTACCAGAACTGACTCTTGGAGTGATTCCTGGTTTTGGAGGAACCCAACGTCTTCCAAGGTTAGTAGGCCTTGCAAAAGCAACGGATATGATACTGCTTTCTAAGTCAATATCCTCAGAAGAGGGGCACAAACTGGGTCTCATTGATGCCTTGGTGCCACCTGGAGATGTGTTGAGCACATCCAGAAAGTGGGCTCTAGACATTGCAGAGGGACGTAAACCCTTTCTGCAGTCACTGCACAGAACCGACAAAATTGGTTCTTTATCTGAAGCTCGTGCTATATTGAAGAATTCAAGACAGCTAGCCAAGAAGATTGCCCCAAATATGCCTCAGCACCATGCTTGCATTGAGGTGATTGAAGAAGGAATCATCCATGGAGGATACAGTGGGGTTCTCAAGGAAGCAGAAGTTTTCAAGCAACTAGTACTGTCAGACACTGCAAAGGGTCTTGTTCATGTTTTCTTTGCACAACGTGCAACATCAAAAGTGCCTAATGTAACTGACGTTGGATTGAAACCAAGGCCGATAAAAAAAGTTGCAGTTATTGGTGGCGGTCTGATGGGTTCAGGCATCGCTACGGCTCTACTTCTAAGCAATATAAGAGTTGTGCTCAAAGAAATAAACTCAGAATTCCTTATGAAGGGAATAAAATCAGTTGAAGCAAATATGAAAAGCTTGGTATCTAGGGGGAAACTGACACAAGATAAAGCAGGAAAGGCCCTCTCTTTGTTCAAGGGAGTACTTGATTACACAGAATTCAATGATGTGGACATGGTCATAGAGGCAGTGATTGAAAACATTCAATTGAAACAAAACATATTCAAAGAAATCGAAAAGGTTTGTTCACCCCACTGCATTTTGGCGAGTAATACATCTACTATCGACCTCGATGTAATTGGTGAAAAGACTAACTCAAAAGATCGCATTGTTGGCGCACATTTCTTCAGTCCGGCCCATCTGATGCCCCTTCTCGAGATTGTTCGTTCAAAGAATACTTCTGCTCAGGTGATTCTGGATCTCATGGCGGTTGGAAAGGCCATAAAGAAAGTTCCCGTGGTGGTTGGAAACTGCATAGGCTTTGCAGTGAATAGGACATTCTTTCCTTATTCACAAGCTGCACATATGTTGGCCAATCTAGGTGTTGACTTGTTCAGAATTGACAGCGTTATTACCTCTTTCGGCTTGCCATTGGGCCCTTTCCAGCTTGGTGATTTGGCTGGACACGGGATTGGATTAGCAGTTGGGCCGATATATGCCAAGGTCTATGGTGACCGCATGTTCAGATCTCCAATGACAGAGCTTCTTCTTAAGAGCGGGCGAAATGGAAAAATCAACGGAAGAGGATACTATATATACGAAAAGGGAAGCAAACCAAAACCTGATCCATCAGTGCTTTCAATTGTTGAGAAATCAAGGAAACTGACCAATATCATGCCTGGTGGGAAGCCTATATCAGTAACTGATAAAGAAATTGTAGAGATGATCTTATTCCCTGTTGTCAACGAGGCATGCCGCGTCCTAGATGAAGGAGTTGTGATCCGAGCCTCAGACTTGGACATTGCGTCTGTCCTTGGAATGAGTTTTCCTTCTTACCGGGGAGGAATTGTTTTCTGGGCAGACACTGTTGGACCAAAGTACATATATGAGAGGCTCAAGAAATTGTCGGAGACTTATGGCAGCTTTTTCAAACCATCGAGGTATCTGGAGGAAAGAGCAATGAATGGAATGCTTTTGAGTGAATCGAAATCGTCGAGGTCCAAATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003857 GO:0004300 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

721

Amino Acids

77.86

Weight (kDa)

9.36

Isoelectric Point (pI)

32.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	13 - 212	4.3e-43	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	18 - 190	5.8e-21	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	311 - 489	1.7e-60	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	492 - 585	7.4e-20	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	553
AccB7I	CCANNNNNTGG	1 cut(s)	588
AccBSI	CCGCTC	1 cut(s)	1713
AccII	CGCG	1 cut(s)	1914
AciI	CCGC	5 cut(s)	951, 1424, 1672, 1713, 1912
AclWI	GGATC	3 cut(s)	1422, 1775, 1930
AcoI	YGGCCR	1 cut(s)	1527
AcsI	RAATTY	3 cut(s)	688, 1028, 1154
AcuI	CTGAAG	3 cut(s)	273, 687, 1327
AcyI	GRCGYC	1 cut(s)	129
AdeI	CACNNNGTG	1 cut(s)	872
AfaI	GTAC	3 cut(s)	825, 1140, 2036
AfiI	CCNNNNNNNGG	4 cut(s)	138, 434, 588, 1039
AflII	CTTAAG	1 cut(s)	1706
AhdI	GACNNNNNGTC	1 cut(s)	1960
AhlI	ACTAGT	1 cut(s)	820
AjnI	CCWGG	4 cut(s)	293, 427, 559, 1834
AjuI	GAANNNNNNNTTGG	2 cut(s)	399, 431
Alw21I	GWGCWC	2 cut(s)	578, 1011
Alw26I	GTCTC	2 cut(s)	542, 2060
AlwI	GGATC	3 cut(s)	1422, 1775, 1930
AlwNI	CAGNNNCTG	4 cut(s)	634, 836, 1859, 2021
Ama87I	CYCGRG	1 cut(s)	1370
AoxI	GGCC	9 cut(s)	153, 466, 920, 1116, 1350, 1434, 1527, 1591, 1643
ApaI	GGGCCC	1 cut(s)	1595
ApeKI	GCWGC	4 cut(s)	311, 377, 1514, 2076
ApoI	RAATTY	3 cut(s)	688, 1028, 1154
Asp700I	GAANNNNTTC	2 cut(s)	184, 1197
AspLEI	GCGC	1 cut(s)	1334
AspS9I	GGNCC	7 cut(s)	1117, 1351, 1591, 1592, 1643, 2027, 2154
AsuC2I	CCSGG	1 cut(s)	1994
AsuHPI	GGTGA	7 cut(s)	49, 763, 1236, 1310, 1417, 1619, 1679
AsuNHI	GCTAGC	1 cut(s)	700
AvaI	CYCGRG	1 cut(s)	1370
AvaII	GGWCC	2 cut(s)	2027, 2154
BaeGI	GKGCMC	2 cut(s)	528, 1595
BalI	TGGCCA	1 cut(s)	1529
BanI	GGYRCC	1 cut(s)	553
BanII	GRGCYC	2 cut(s)	595, 1595
BauI	CACGAG	1 cut(s)	672
BbsI	GAAGAC	2 cut(s)	294, 443
Bbv12I	GWGCWC	2 cut(s)	578, 1011
BbvCI	CCTCAGC	1 cut(s)	729
BbvI	GCAGC	4 cut(s)	298, 364, 1501, 2088
BccI	CCATC	8 cut(s)	19, 35, 164, 277, 952, 1362, 1792, 2098
BceAI	ACGGC	1 cut(s)	993
BcgI	CGANNNNNNTGC	2 cut(s)	29, 63
BciT130I	CCWGG	4 cut(s)	295, 429, 561, 1836
BciVI	GTATCC	2 cut(s)	773, 1736
BcnI	CCSGG	1 cut(s)	1994
BcoDI	GTCTC	2 cut(s)	542, 2060
BcuI	ACTAGT	1 cut(s)	820
BfaI	CTAG	6 cut(s)	596, 701, 821, 1083, 1535, 1919
BfmI	CTRYAG	2 cut(s)	312, 626
BfrI	CTTAAG	1 cut(s)	1706
BfuI	GTATCC	2 cut(s)	773, 1736
BglII	AGATCT	1 cut(s)	1682
BisI	GCNGC	5 cut(s)	312, 378, 1515, 1912, 2077
BlsI	GCNGC	5 cut(s)	313, 379, 1516, 1913, 2078
BmcAI	AGTACT	2 cut(s)	825, 1140
Bme1390I	CCNGG	5 cut(s)	295, 429, 561, 1836, 1994
Bme18I	GGWCC	2 cut(s)	2027, 2154
BmeRI	GACNNNNNGTC	1 cut(s)	1960
BmeT110I	CYCGRG	1 cut(s)	1370
BmgT120I	GGNCC	7 cut(s)	1117, 1351, 1591, 1592, 1643, 2027, 2154
BmiI	GGNNCC	3 cut(s)	442, 555, 1593
BmrFI	CCNGG	5 cut(s)	295, 429, 561, 1836, 1994
BmrI	ACTGGG	2 cut(s)	240, 542
BmsI	GCATC	3 cut(s)	535, 978, 1350
BmtI	GCTAGC	1 cut(s)	704
BmuI	ACTGGG	2 cut(s)	240, 542
BpiI	GAAGAC	2 cut(s)	294, 443
BplI	GAGNNNNNCTC	2 cut(s)	993, 1025
BpmI	CTGGAG	3 cut(s)	277, 582, 2123
Bpu10I	CCTNAGC	2 cut(s)	729, 1401
BpuEI	CTTGAG	2 cut(s)	779, 2036
BpuMI	CCSGG	1 cut(s)	1994
BsaHI	GRCGYC	1 cut(s)	129
BsaI	GGTCTC	1 cut(s)	542
BsaJI	CCNNGG	8 cut(s)	455, 549, 772, 917, 1452, 1656, 1969, 1993
BsaXI	ACNNNNNCTCC	2 cut(s)	1989, 2019
Bsc4I	CCNNNNNNNGG	4 cut(s)	138, 434, 588, 1039
Bse118I	RCCGGY	1 cut(s)	301
Bse1I	ACTGG	3 cut(s)	90, 235, 537
Bse3DI	GCAATG	4 cut(s)	360, 600, 1956, 2121
BseBI	CCWGG	4 cut(s)	295, 429, 561, 1836
BseDI	CCNNGG	8 cut(s)	455, 549, 772, 917, 1452, 1656, 1969, 1993
BseGI	GGATG	2 cut(s)	578, 768
BseLI	CCNNNNNNNGG	4 cut(s)	138, 434, 588, 1039
BseMI	GCAATG	4 cut(s)	360, 600, 1956, 2121
BseMII	CTCAG	5 cut(s)	528, 743, 1038, 1415, 1959
BseNI	ACTGG	3 cut(s)	90, 235, 537
BseSI	GKGCMC	2 cut(s)	528, 1595
BseXI	GCAGC	4 cut(s)	298, 364, 1501, 2088
BsgI	GTGCAG	2 cut(s)	620, 1500
Bsh1236I	CGCG	1 cut(s)	1914
BshFI	GGCC	9 cut(s)	155, 468, 922, 1118, 1352, 1436, 1529, 1593, 1645
BshNI	GGYRCC	1 cut(s)	553
BsiHKAI	GWGCWC	2 cut(s)	578, 1011
BsiHKCI	CYCGRG	1 cut(s)	1370
BsiSI	CCGG	3 cut(s)	302, 1349, 1993
BslFI	GGGAC	1 cut(s)	624
BslI	CCNNNNNNNGG	4 cut(s)	138, 434, 588, 1039
BsmAI	GTCTC	2 cut(s)	542, 2060
BsmFI	GGGAC	1 cut(s)	624
BsmI	GAATGC	1 cut(s)	2130
BsnI	GGCC	9 cut(s)	155, 468, 922, 1118, 1352, 1436, 1529, 1593, 1645
Bso31I	GGTCTC	1 cut(s)	542
BsoBI	CYCGRG	1 cut(s)	1370
Bsp120I	GGGCCC	1 cut(s)	1591
Bsp1286I	GDGCHC	5 cut(s)	528, 578, 595, 1011, 1595
Bsp143I	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
Bsp19I	CCATGG	1 cut(s)	772
BspACI	CCGC	5 cut(s)	951, 1424, 1672, 1713, 1912
BspANI	GGCC	9 cut(s)	155, 468, 922, 1118, 1352, 1436, 1529, 1593, 1645
BspCNI	CTCAG	5 cut(s)	527, 742, 1037, 1414, 1958
BspFNI	CGCG	1 cut(s)	1914
BspLI	GGNNCC	3 cut(s)	442, 555, 1593
BspMAI	CTGCAG	2 cut(s)	316, 630
BspOI	GCTAGC	1 cut(s)	704
BspPI	GGATC	3 cut(s)	1422, 1775, 1930
BspT107I	GGYRCC	1 cut(s)	553
BspTI	CTTAAG	1 cut(s)	1706
BspTNI	GGTCTC	1 cut(s)	542
BsrBI	CCGCTC	1 cut(s)	1713
BsrDI	GCAATG	4 cut(s)	360, 600, 1956, 2121
BsrFI	RCCGGY	1 cut(s)	301
BsrI	ACTGG	3 cut(s)	90, 235, 537
BssAI	RCCGGY	1 cut(s)	301
BssECI	CCNNGG	8 cut(s)	455, 549, 772, 917, 1452, 1656, 1969, 1993
BssMI	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
BssNI	GRCGYC	1 cut(s)	129
BssSI	CACGAG	1 cut(s)	672
BssT1I	CCWWGG	6 cut(s)	455, 549, 772, 917, 1656, 1969
Bst2BI	CACGAG	1 cut(s)	672
Bst2UI	CCWGG	4 cut(s)	295, 429, 561, 1836
Bst4CI	ACNGT	3 cut(s)	785, 828, 2023
Bst6I	CTCTTC	2 cut(s)	513, 1732
BstACI	GRCGYC	1 cut(s)	129
BstAFI	CTTAAG	1 cut(s)	1706
BstAPI	GCANNNNNTGC	2 cut(s)	634, 872
BstC8I	GCNNGC	5 cut(s)	702, 742, 1583, 1715, 1909
BstDEI	CTNAG	7 cut(s)	501, 514, 729, 989, 1024, 1401, 1945
BstDSI	CCRYGG	2 cut(s)	772, 1452
BstEII	GGTNACC	1 cut(s)	1667
BstENI	CCTNNNNNAGG	1 cut(s)	1037
BstF5I	GGATG	2 cut(s)	578, 768
BstFNI	CGCG	1 cut(s)	1914
BstHHI	GCGC	1 cut(s)	1334
BstKTI	GATC	7 cut(s)	168, 1321, 1417, 1685, 1783, 1884, 1938
BstMAI	GTCTC	2 cut(s)	542, 2060
BstMBI	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
BstMWI	GCNNNNNNNGC	7 cut(s)	365, 374, 386, 634, 872, 1115, 1329
BstNI	CCWGG	4 cut(s)	295, 429, 561, 1836
BstNSI	RCATGY	2 cut(s)	1678, 1911
BstPI	GGTNACC	1 cut(s)	1667
BstSCI	CCNGG	5 cut(s)	293, 427, 559, 1834, 1992
BstSFI	CTRYAG	2 cut(s)	312, 626
BstSLI	GKGCMC	2 cut(s)	528, 1595
BstUI	CGCG	1 cut(s)	1914
BstV1I	GCAGC	4 cut(s)	298, 364, 1501, 2088
BstV2I	GAAGAC	2 cut(s)	294, 443
BstX2I	RGATCY	2 cut(s)	1414, 1682
BstYI	RGATCY	2 cut(s)	1414, 1682
BsuI	GTATCC	2 cut(s)	773, 1736
BsuRI	GGCC	9 cut(s)	155, 468, 922, 1118, 1352, 1436, 1529, 1593, 1645
BtgI	CCRYGG	2 cut(s)	772, 1452
BtgZI	GCGATG	1 cut(s)	955
BtsCI	GGATG	2 cut(s)	578, 768
BtsI	GCAGTG	5 cut(s)	632, 834, 1191, 1249, 1488
BtsIMutI	CAGTG	8 cut(s)	632, 790, 834, 1191, 1249, 1488, 1794, 2019
Cac8I	GCNNGC	5 cut(s)	702, 742, 1583, 1715, 1909
CaiI	CAGNNNCTG	4 cut(s)	634, 836, 1859, 2021
CfoI	GCGC	1 cut(s)	1334
Cfr10I	RCCGGY	1 cut(s)	301
Cfr13I	GGNCC	7 cut(s)	1117, 1351, 1591, 1592, 1643, 2027, 2154
CseI	GACGC	3 cut(s)	137, 1903, 1950
Csp6I	GTAC	3 cut(s)	824, 1139, 2035
CviAII	CATG	9 cut(s)	365, 737, 773, 854, 1171, 1420, 1675, 1831, 1908
CviQI	GTAC	3 cut(s)	824, 1139, 2035
DdeI	CTNAG	7 cut(s)	501, 514, 729, 989, 1024, 1401, 1945
DpnI	GATC	7 cut(s)	167, 1320, 1416, 1684, 1782, 1883, 1937
DpnII	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
DraIII	CACNNNGTG	1 cut(s)	872
DriI	GACNNNNNGTC	1 cut(s)	1960
EaeI	YGGCCR	1 cut(s)	1527
Eam1104I	CTCTTC	2 cut(s)	513, 1732
Eam1105I	GACNNNNNGTC	1 cut(s)	1960
EarI	CTCTTC	2 cut(s)	513, 1732
Eco130I	CCWWGG	6 cut(s)	455, 549, 772, 917, 1656, 1969
Eco147I	AGGCCT	1 cut(s)	468
Eco24I	GRGCYC	2 cut(s)	595, 1595
Eco31I	GGTCTC	1 cut(s)	542
Eco32I	GATATC	1 cut(s)	202
Eco47I	GGWCC	2 cut(s)	2027, 2154
Eco57I	CTGAAG	3 cut(s)	273, 687, 1327
Eco88I	CYCGRG	1 cut(s)	1370
Eco91I	GGTNACC	1 cut(s)	1667
EcoNI	CCTNNNNNAGG	1 cut(s)	1037
EcoO109I	RGGNCCY	2 cut(s)	1117, 1592
EcoO65I	GGTNACC	1 cut(s)	1667
EcoRI	GAATTC	3 cut(s)	688, 1028, 1154
EcoRII	CCWGG	4 cut(s)	293, 427, 559, 1834
EcoRV	GATATC	1 cut(s)	202
EcoT14I	CCWWGG	6 cut(s)	455, 549, 772, 917, 1656, 1969
EcoT38I	GRGCYC	2 cut(s)	595, 1595
ErhI	CCWWGG	6 cut(s)	455, 549, 772, 917, 1656, 1969
FaeI	CATG	9 cut(s)	368, 740, 776, 857, 1174, 1423, 1678, 1834, 1911
FaqI	GGGAC	1 cut(s)	624
FatI	CATG	9 cut(s)	364, 736, 772, 853, 1170, 1419, 1674, 1830, 1907
FauI	CCCGC	1 cut(s)	1706
FauNDI	CATATG	1 cut(s)	1521
Fnu4HI	GCNGC	5 cut(s)	312, 378, 1515, 1912, 2077
FokI	GGATG	2 cut(s)	565, 755
FriOI	GRGCYC	2 cut(s)	595, 1595
Fsp4HI	GCNGC	5 cut(s)	312, 378, 1515, 1912, 2077
FspBI	CTAG	6 cut(s)	596, 701, 821, 1083, 1535, 1919
GlaI	GCGC	1 cut(s)	1333
GluI	GCNGC	5 cut(s)	312, 378, 1515, 1912, 2077
GsuI	CTGGAG	3 cut(s)	277, 582, 2123
HaeIII	GGCC	9 cut(s)	155, 468, 922, 1118, 1352, 1436, 1529, 1593, 1645
HapII	CCGG	3 cut(s)	302, 1349, 1993
HgaI	GACGC	3 cut(s)	137, 1903, 1950
HhaI	GCGC	1 cut(s)	1334
Hin1I	GRCGYC	1 cut(s)	129
Hin1II	CATG	9 cut(s)	368, 740, 776, 857, 1174, 1423, 1678, 1834, 1911
Hin6I	GCGC	1 cut(s)	1332
HinP1I	GCGC	1 cut(s)	1332
HincII	GTYRAC	3 cut(s)	79, 1543, 1900
HindII	GTYRAC	3 cut(s)	79, 1543, 1900
HindIII	AAGCTT	1 cut(s)	1072
HinfI	GANTC	6 cut(s)	290, 412, 424, 765, 1408, 2138
HpaI	GTTAAC	1 cut(s)	79
HpaII	CCGG	3 cut(s)	302, 1349, 1993
HphI	GGTGA	7 cut(s)	49, 763, 1236, 1310, 1417, 1619, 1679
Hpy166II	GTNNAC	6 cut(s)	79, 617, 1168, 1244, 1543, 1900
Hpy8I	GTNNAC	6 cut(s)	79, 617, 1168, 1244, 1543, 1900
Hpy99I	CGWCG	1 cut(s)	2152
HpyAV	CCTTC	7 cut(s)	106, 314, 755, 1033, 1376, 1919, 1995
HpyCH4III	ACNGT	3 cut(s)	785, 828, 2023
HpyCH4IV	ACGT	4 cut(s)	448, 613, 871, 903
HpyF10VI	GCNNNNNNNGC	7 cut(s)	365, 374, 386, 634, 872, 1115, 1329
HpyF3I	CTNAG	7 cut(s)	501, 514, 729, 989, 1024, 1401, 1945
HpySE526I	ACGT	4 cut(s)	448, 613, 871, 903
Hsp92I	GRCGYC	1 cut(s)	129
Hsp92II	CATG	9 cut(s)	368, 740, 776, 857, 1174, 1423, 1678, 1834, 1911
HspAI	GCGC	1 cut(s)	1332
KspAI	GTTAAC	1 cut(s)	79
Kzo9I	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
LmnI	GCTCC	1 cut(s)	385
Lsp1109I	GCAGC	4 cut(s)	298, 364, 1501, 2088
LweI	GCATC	3 cut(s)	535, 978, 1350
MaeI	CTAG	6 cut(s)	596, 701, 821, 1083, 1535, 1919
MaeII	ACGT	4 cut(s)	448, 613, 871, 903
MaeIII	GTNAC	7 cut(s)	19, 125, 275, 630, 895, 1667, 1855
MalI	GATC	7 cut(s)	167, 1320, 1416, 1684, 1782, 1883, 1937
MbiI	CCGCTC	1 cut(s)	1713
MboI	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
MfeI	CAATTG	2 cut(s)	1202, 1797
MflI	RGATCY	2 cut(s)	1414, 1682
MhlI	GDGCHC	5 cut(s)	528, 578, 595, 1011, 1595
MlsI	TGGCCA	1 cut(s)	1529
MluNI	TGGCCA	1 cut(s)	1529
MlyI	GAGTC	2 cut(s)	284, 406
MmeI	TCCRAC	5 cut(s)	886, 1408, 1441, 2005, 2043
Mox20I	TGGCCA	1 cut(s)	1529
MroXI	GAANNNNTTC	2 cut(s)	184, 1197
MscI	TGGCCA	1 cut(s)	1529
MseI	TTAA	3 cut(s)	78, 150, 1707
MslI	CAYNNNNRTG	1 cut(s)	749
Msp20I	TGGCCA	1 cut(s)	1529
MspCI	CTTAAG	1 cut(s)	1706
MspI	CCGG	3 cut(s)	302, 1349, 1993
MspR9I	CCNGG	5 cut(s)	295, 429, 561, 1836, 1994
MunI	CAATTG	2 cut(s)	1202, 1797
Mva1269I	GAATGC	1 cut(s)	2130
MvaI	CCWGG	4 cut(s)	295, 429, 561, 1836
MvnI	CGCG	1 cut(s)	1914
MwoI	GCNNNNNNNGC	7 cut(s)	365, 374, 386, 634, 872, 1115, 1329
NciI	CCSGG	1 cut(s)	1994
NcoI	CCATGG	1 cut(s)	772
NdeI	CATATG	1 cut(s)	1521
NdeII	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
NheI	GCTAGC	1 cut(s)	700
NlaIII	CATG	9 cut(s)	368, 740, 776, 857, 1174, 1423, 1678, 1834, 1911
NlaIV	GGNNCC	3 cut(s)	442, 555, 1593
NmuCI	GTSAC	3 cut(s)	125, 630, 1667
NspI	RCATGY	2 cut(s)	1678, 1911
PaeI	GCATGC	1 cut(s)	1911
PaeR7I	CTCGAG	1 cut(s)	1370
PceI	AGGCCT	1 cut(s)	468
PcsI	WCGNNNNNNNCGW	1 cut(s)	2147
PctI	GAATGC	1 cut(s)	2130
PdmI	GAANNNNTTC	2 cut(s)	184, 1197
PfeI	GAWTC	4 cut(s)	424, 765, 1408, 2138
PflFI	GACNNNGTC	1 cut(s)	1172
PflMI	CCANNNNNTGG	1 cut(s)	588
PfoI	TCCNGGA	1 cut(s)	293
PkrI	GCNGC	5 cut(s)	313, 379, 1516, 1913, 2078
PleI	GAGTC	2 cut(s)	284, 406
PpsI	GAGTC	2 cut(s)	284, 406
Psp6I	CCWGG	4 cut(s)	293, 427, 559, 1834
PspEI	GGTNACC	1 cut(s)	1667
PspGI	CCWGG	4 cut(s)	293, 427, 559, 1834
PspN4I	GGNNCC	3 cut(s)	442, 555, 1593
PspOMI	GGGCCC	1 cut(s)	1591
PspPI	GGNCC	7 cut(s)	1117, 1351, 1591, 1592, 1643, 2027, 2154
PstI	CTGCAG	2 cut(s)	316, 630
PstNI	CAGNNNCTG	4 cut(s)	634, 836, 1859, 2021
PsuI	RGATCY	2 cut(s)	1414, 1682
PsyI	GACNNNGTC	1 cut(s)	1172
RsaI	GTAC	3 cut(s)	825, 1140, 2036
RsaNI	GTAC	3 cut(s)	824, 1139, 2035
RseI	CAYNNNNRTG	1 cut(s)	749
SaqAI	TTAA	3 cut(s)	78, 150, 1707
SatI	GCNGC	5 cut(s)	312, 378, 1515, 1912, 2077
Sau3AI	GATC	7 cut(s)	165, 1318, 1414, 1682, 1780, 1881, 1935
Sau96I	GGNCC	7 cut(s)	1117, 1351, 1591, 1592, 1643, 2027, 2154
ScaI	AGTACT	2 cut(s)	825, 1140
SchI	GAGTC	2 cut(s)	284, 406
ScrFI	CCNGG	5 cut(s)	295, 429, 561, 1836, 1994
SduI	GDGCHC	5 cut(s)	528, 578, 595, 1011, 1595
SfaNI	GCATC	3 cut(s)	535, 978, 1350
SfcI	CTRYAG	2 cut(s)	312, 626
Sfr274I	CTCGAG	1 cut(s)	1370
SinI	GGWCC	2 cut(s)	2027, 2154
SlaI	CTCGAG	1 cut(s)	1370
SmiMI	CAYNNNNRTG	1 cut(s)	749
SmlI	CTYRAG	4 cut(s)	794, 1370, 1706, 2051
SmoI	CTYRAG	4 cut(s)	794, 1370, 1706, 2051
SpeI	ACTAGT	1 cut(s)	820
SphI	GCATGC	1 cut(s)	1911
SseBI	AGGCCT	1 cut(s)	468
SsiI	CCGC	5 cut(s)	951, 1424, 1672, 1713, 1912
SspMI	CTAG	6 cut(s)	596, 701, 821, 1083, 1535, 1919
StuI	AGGCCT	1 cut(s)	468
StyD4I	CCNGG	5 cut(s)	293, 427, 559, 1834, 1992
StyI	CCWWGG	6 cut(s)	455, 549, 772, 917, 1656, 1969
TaaI	ACNGT	3 cut(s)	785, 828, 2023
TaiI	ACGT	4 cut(s)	451, 616, 874, 906
TaqI	TCGA	7 cut(s)	1230, 1281, 1287, 1371, 2093, 2141, 2150
TatI	WGTACW	3 cut(s)	823, 1138, 2034
TauI	GCSGC	1 cut(s)	1914
TfiI	GAWTC	4 cut(s)	424, 765, 1408, 2138
Tru1I	TTAA	3 cut(s)	78, 150, 1707
Tru9I	TTAA	3 cut(s)	78, 150, 1707
TscAI	CASTG	8 cut(s)	639, 790, 841, 1191, 1256, 1488, 1794, 2026
TseFI	GTSAC	3 cut(s)	125, 630, 1667
TseI	GCWGC	4 cut(s)	311, 377, 1514, 2076
Tsp45I	GTSAC	3 cut(s)	125, 630, 1667
TspDTI	ATGAA	6 cut(s)	212, 842, 1052, 1082, 1938, 2132
TspGWI	ACGGA	2 cut(s)	497, 1749
TspRI	CASTG	8 cut(s)	639, 790, 841, 1191, 1256, 1488, 1794, 2026
Tth111I	GACNNNGTC	1 cut(s)	1172
Van91I	CCANNNNNTGG	1 cut(s)	588
Vha464I	CTTAAG	1 cut(s)	1706
VpaK11BI	GGWCC	2 cut(s)	2027, 2154
XagI	CCTNNNNNAGG	1 cut(s)	1037
XapI	RAATTY	3 cut(s)	688, 1028, 1154
XbaI	TCTAGA	1 cut(s)	595
XceI	RCATGY	2 cut(s)	1678, 1911
XhoI	CTCGAG	1 cut(s)	1370
XmnI	GAANNNNTTC	2 cut(s)	184, 1197
XspI	CTAG	6 cut(s)	596, 701, 821, 1083, 1535, 1919
ZrmI	AGTACT	2 cut(s)	825, 1140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.