Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Forward (+)

3439832 .. 3444998

Gene Structure 5167 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG048500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGCGGGCGTCAAGGTCACCTTGGAGGTCGGAAGCGACGGTGTCGCCGTCATCACCATGTTCAATCCTCCCGTCAACGCCTTGGCCATATCCATTCTCAAGGGGCTGAAGGAGAAGTTCGATGAGGCCGCGAGGAGAAACGACGTCAAGGCTATTGTCGTTACTGGAAATGGAGGAAGGTTTTCTGGAGGTTTTGATATCAACGTTTTCGAGAAGGTTCACGCTACCGGGGATGTTTCGCTCATGCCTGATGTATCTGTTGACTTGATGGTCAACACTGTTGAAGATTCGAAGAAGCCTGTTGTTGCTGCGGGATGCCATGCACGCATTGCTGCTCCAAGAACTCAACTAGGCCTGCCAGAGTTGACACTTGGTGTTATTCCGGGATTTGGAGGCACACAGCGTCTTCCAAGGCTTGTGGGGCTTCCGAAGGCAATTGAGATGATGCTGTTATCCAAGTCAATCATGTCTGAAGAAGGGGCAAAGCTTGGTCTTATCGATGCTATTGTGCCTTCTCAAGAGTTGCTGAAAGTATCTCGATTATGGGCTTTGGACATTGCAGCGGCGCGAAAACCATGGTTACGGTCCCTTCATAGGACAGACAAGCTTTGCTCCCTATCTGAGGCACGTGAGATAATTAAAGTTGCTAGACAACAAGCCAGAACGACGGCTCCCAATATGCCTCAGCATCAAGTATGCCTCGATGCGATTGAGGAGGGCATTATTCATGGAGGATATAGTGGAGTTCTGAAGGAAGCTAAGGTTTTTAAGGAGTTAGTTGTATCAGACACTTCAAAAGGTCTTGTACATGTATTTTTTGCCCAACGGGCAACATCAAAGGTGCCCAAGGTTACTGATGTTGGGCTTAAACCAAGGCATATAAAGAAGGTTGCTATCATTGGGGGAGGTCTGATGGGTTCTGGCATAGCTACAGCCCTTCTTCTGAGCACTGTATCTGTTGTTCTCAAAGAAATCAACTCTGAATTTCTTCAGAAGGGTGTACGAACAATAGAAGGAAATGTTAAAGGCTTGGTAACTAGAGGGAAGCTGACACGGGATAAGGCAGACAAAGCTCTCTCATTGCTTAAAGGTAGTCTGGACTACTCAGACTTCAAAGATGTGGATATGGTCATAGAGGCTGTCATTGAGAGCGTTCCTCTCAAACAAAAGATTTTTGGTGAACTTGAGAAAGTTTGCCCTTCTCATTGCATTTTGGCTACAAACACATCCACTATTGACCTCAATGTAGTTGGAGAAAAGACGAGATCTCAGGATCGCATCGTGGGGGCACACTTTTTCAGTCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCGGCACAAGTAATTCTTGACCTTTTGACGGTTGGAAAAGTAATAAAGAAAGTTCCTGTTGTGGTTGGTAACTGTACGGGATTTGCAGTCAATCGGGCATTCTTTCCCTATACCCAAGGCGCACATATATTGGTCAATTCAGGTGTCGATGTGTTCAGGATTGACAGGATCATCAGCAATTTTGGCCTCCCTATGGGACCATTCCAACTTCAGGATTTGGCAGGCTATGGAGTAGCTCTTGCTGTTGGAAAGATATTTGCTAGTGCTTTCCCTGATCGCACATTTCGATCACCATTGGTCGAACTCTTAGTCAAAAATGGACGAAATGGTAAAAACAATGGAAAAGGATATTACACTTATGAAAAGGGAAGCAAGCCCAAGCCCGATCTGTCTGTGCTACCAATTATTGAGGAGTCTAGACGAATTACCAATATGATGCCTGGTGGAAAGCCTTTATCTGTTACAGACCAAGAAATTCTGGAAATAATACTCTTTCCTGTGGTGAATGAGGCATGTCGTATTTTGGATGAAGGAATAGTTATTCGAGCAGCAGACCTTGACATTGCATCTGTTCTTGGCATGAGTTTCCCAAATTACCGGGGTGGCATTGTCTTTTGGGCAGATATGGTTGGGCCTAAGCATATATACACTACTCTCAAGAAATGGTCGGGAGTATACGGTGATTTCTTCAAACCATCAAGGTTCTTGGAGGAAAGAGCGGCAAAAGGCTTGCCTCTGAGTGCACTCGCATCATCCTCATCTTCAAGGTCACGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

709

Amino Acids

76.68

Weight (kDa)

9.45

Isoelectric Point (pI)

32.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	13 - 197	3.1e-25	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 185	5.1e-10	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	296 - 474	2.9e-58	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	477 - 570	2.4e-22	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	269
AatII	GACGTC	1 cut(s)	147
AccB1I	GGYRCC	1 cut(s)	841
AccBSI	CCGCTC	1 cut(s)	2069
AccI	GTMKAC	1 cut(s)	2025
AccII	CGCG	2 cut(s)	131, 568
AciI	CCGC	5 cut(s)	5, 129, 311, 563, 2069
AclI	AACGTT	1 cut(s)	204
AclWI	GGATC	2 cut(s)	1281, 1526
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	2 cut(s)	983, 1824
AcuI	CTGAAG	5 cut(s)	128, 492, 770, 974, 1544
AcvI	CACGTG	1 cut(s)	629
AcyI	GRCGYC	2 cut(s)	9, 144
AdeI	CACNNNGTG	1 cut(s)	374
AfaI	GTAC	4 cut(s)	807, 1002, 1335, 1426
AfiI	CCNNNNNNNGG	6 cut(s)	389, 594, 622, 1378, 1543, 1561
AflIII	ACRYGT	1 cut(s)	808
AgsI	TTSAA	7 cut(s)	64, 284, 795, 1114, 1328, 2041, 2115
AjnI	CCWGG	1 cut(s)	1789
AloI	GAACNNNNNNTCC	2 cut(s)	655, 687
AluBI	AGCT	7 cut(s)	487, 607, 758, 929, 1048, 1073, 1586
AluI	AGCT	7 cut(s)	487, 607, 758, 929, 1048, 1073, 1586
Alw21I	GWGCWC	2 cut(s)	950, 2095
Alw44I	GTGCAC	1 cut(s)	2091
AlwI	GGATC	2 cut(s)	1281, 1526
AoxI	GGCC	5 cut(s)	84, 126, 352, 1534, 1982
ApaLI	GTGCAC	1 cut(s)	2091
ApeKI	GCWGC	4 cut(s)	308, 332, 560, 1898
ApoI	RAATTY	2 cut(s)	983, 1824
ArsI	GACNNNNNNTTYG	2 cut(s)	1644, 1676
Asp700I	GAANNNNTTC	2 cut(s)	181, 987
AspLEI	GCGC	2 cut(s)	568, 1472
AspS9I	GGNCC	3 cut(s)	585, 1547, 1982
AsuC2I	CCSGG	3 cut(s)	229, 384, 1949
AsuHPI	GGTGA	6 cut(s)	10, 46, 1190, 1632, 1864, 2042
AsuII	TTCGAA	1 cut(s)	290
AvaII	GGWCC	2 cut(s)	585, 1547
BaeGI	GKGCMC	3 cut(s)	846, 1291, 2095
BalI	TGGCCA	1 cut(s)	86
BanI	GGYRCC	1 cut(s)	841
BbrPI	CACGTG	1 cut(s)	629
BbsI	GAAGAC	2 cut(s)	398, 1352
Bbv12I	GWGCWC	2 cut(s)	950, 2095
BbvCI	CCTCAGC	1 cut(s)	684
BbvI	GCAGC	4 cut(s)	295, 319, 572, 1910
BccI	CCATC	3 cut(s)	262, 907, 2053
BceAI	ACGGC	2 cut(s)	32, 684
BciT130I	CCWGG	1 cut(s)	1791
BcnI	CCSGG	3 cut(s)	229, 384, 1949
BfaI	CTAG	5 cut(s)	350, 648, 1038, 1611, 1767
BfmI	CTRYAG	1 cut(s)	930
BglI	GCCNNNNNGGC	1 cut(s)	827
BglII	AGATCT	1 cut(s)	1265
BisI	GCNGC	7 cut(s)	129, 309, 333, 561, 564, 1899, 2070
BlsI	GCNGC	7 cut(s)	130, 310, 334, 562, 565, 1900, 2071
Bme1390I	CCNGG	4 cut(s)	229, 384, 1791, 1949
Bme18I	GGWCC	2 cut(s)	585, 1547
BmgT120I	GGNCC	3 cut(s)	585, 1547, 1982
BmiI	GGNNCC	4 cut(s)	587, 672, 843, 1548
BmrFI	CCNGG	4 cut(s)	229, 384, 1791, 1949
BpiI	GAAGAC	2 cut(s)	398, 1352
BplI	GAGNNNNNCTC	2 cut(s)	1141, 1173
BpmI	CTGGAG	1 cut(s)	207
Bpu10I	CCTNAGC	3 cut(s)	684, 759, 1986
Bpu14I	TTCGAA	1 cut(s)	290
BpuEI	CTTGAG	4 cut(s)	83, 501, 1205, 1991
BpuMI	CCSGG	3 cut(s)	229, 384, 1949
Bsa29I	ATCGAT	1 cut(s)	498
BsaAI	YACGTR	1 cut(s)	629
BsaHI	GRCGYC	2 cut(s)	9, 144
BsaJI	CCNNGG	9 cut(s)	21, 81, 228, 410, 575, 846, 872, 1465, 1948
BsaXI	ACNNNNNCTCC	2 cut(s)	655, 685
Bsc4I	CCNNNNNNNGG	6 cut(s)	389, 594, 622, 1378, 1543, 1561
Bse1I	ACTGG	1 cut(s)	169
Bse3DI	GCAATG	5 cut(s)	327, 555, 1079, 1204, 1911
BseBI	CCWGG	1 cut(s)	1791
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	9 cut(s)	21, 81, 228, 410, 575, 846, 872, 1465, 1948
BseGI	GGATG	5 cut(s)	238, 320, 1226, 1882, 2102
BseLI	CCNNNNNNNGG	6 cut(s)	389, 594, 622, 1378, 1543, 1561
BseMI	GCAATG	5 cut(s)	327, 555, 1079, 1204, 1911
BseMII	CTCAG	6 cut(s)	612, 698, 935, 1119, 1283, 2078
BseNI	ACTGG	1 cut(s)	169
BseRI	GAGGAG	3 cut(s)	148, 728, 1775
BseSI	GKGCMC	3 cut(s)	846, 1291, 2095
BseXI	GCAGC	4 cut(s)	295, 319, 572, 1910
Bsh1236I	CGCG	2 cut(s)	131, 568
BshFI	GGCC	5 cut(s)	86, 128, 354, 1536, 1984
BshNI	GGYRCC	1 cut(s)	841
BshVI	ATCGAT	1 cut(s)	498
BsiHKAI	GWGCWC	2 cut(s)	950, 2095
BsiSI	CCGG	3 cut(s)	228, 383, 1948
BslFI	GGGAC	2 cut(s)	571, 1560
BslI	CCNNNNNNNGG	6 cut(s)	389, 594, 622, 1378, 1543, 1561
BsmFI	GGGAC	2 cut(s)	571, 1560
BsmI	GAATGC	1 cut(s)	1448
BsnI	GGCC	5 cut(s)	86, 128, 354, 1536, 1984
Bsp119I	TTCGAA	1 cut(s)	290
Bsp1286I	GDGCHC	4 cut(s)	846, 950, 1291, 2095
Bsp1407I	TGTACA	1 cut(s)	805
Bsp143I	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
Bsp19I	CCATGG	1 cut(s)	575
BspACI	CCGC	5 cut(s)	5, 129, 311, 563, 2069
BspANI	GGCC	5 cut(s)	86, 128, 354, 1536, 1984
BspCNI	CTCAG	6 cut(s)	613, 697, 936, 1118, 1282, 2079
BspDI	ATCGAT	1 cut(s)	498
BspFNI	CGCG	2 cut(s)	131, 568
BspLI	GGNNCC	4 cut(s)	587, 672, 843, 1548
BspPI	GGATC	2 cut(s)	1281, 1526
BspT104I	TTCGAA	1 cut(s)	290
BspT107I	GGYRCC	1 cut(s)	841
BsrBI	CCGCTC	1 cut(s)	2069
BsrDI	GCAATG	5 cut(s)	327, 555, 1079, 1204, 1911
BsrGI	TGTACA	1 cut(s)	805
BsrI	ACTGG	1 cut(s)	169
BssECI	CCNNGG	9 cut(s)	21, 81, 228, 410, 575, 846, 872, 1465, 1948
BssMI	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
BssNAI	GTATAC	1 cut(s)	2026
BssNI	GRCGYC	2 cut(s)	9, 144
BssT1I	CCWWGG	7 cut(s)	21, 81, 410, 575, 846, 872, 1465
Bst1107I	GTATAC	1 cut(s)	2026
Bst2UI	CCWGG	1 cut(s)	1791
Bst4CI	ACNGT	7 cut(s)	41, 280, 585, 952, 1381, 1424, 2030
Bst6I	CTCTTC	1 cut(s)	1326
BstACI	GRCGYC	2 cut(s)	9, 144
BstAPI	GCANNNNNTGC	1 cut(s)	329
BstAUI	TGTACA	1 cut(s)	805
BstBAI	YACGTR	1 cut(s)	629
BstBI	TTCGAA	1 cut(s)	290
BstC8I	GCNNGC	6 cut(s)	7, 325, 356, 1573, 1724, 2081
BstDEI	CTNAG	9 cut(s)	621, 684, 759, 944, 1105, 1269, 1657, 1986, 2087
BstDSI	CCRYGG	1 cut(s)	575
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	1 cut(s)	620
BstF5I	GGATG	5 cut(s)	238, 320, 1226, 1882, 2102
BstFNI	CGCG	2 cut(s)	131, 568
BstHHI	GCGC	2 cut(s)	568, 1472
BstKTI	GATC	6 cut(s)	1268, 1276, 1521, 1627, 1640, 1738
BstMBI	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
BstMWI	GCNNNNNNNGC	6 cut(s)	324, 329, 421, 827, 1070, 1533
BstNI	CCWGG	1 cut(s)	1791
BstNSI	RCATGY	3 cut(s)	812, 1866, 2128
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	4 cut(s)	227, 382, 1789, 1947
BstSFI	CTRYAG	1 cut(s)	930
BstSLI	GKGCMC	3 cut(s)	846, 1291, 2095
BstUI	CGCG	2 cut(s)	131, 568
BstV1I	GCAGC	4 cut(s)	295, 319, 572, 1910
BstV2I	GAAGAC	2 cut(s)	398, 1352
BstX2I	RGATCY	1 cut(s)	1265
BstYI	RGATCY	1 cut(s)	1265
BstZ17I	GTATAC	1 cut(s)	2026
Bsu15I	ATCGAT	1 cut(s)	498
BsuRI	GGCC	5 cut(s)	86, 128, 354, 1536, 1984
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	1 cut(s)	575
BtsCI	GGATG	5 cut(s)	238, 320, 1226, 1882, 2102
BtsIMutI	CAGTG	2 cut(s)	276, 948
Cac8I	GCNNGC	6 cut(s)	7, 325, 356, 1573, 1724, 2081
CfoI	GCGC	2 cut(s)	568, 1472
Cfr13I	GGNCC	3 cut(s)	585, 1547, 1982
ClaI	ATCGAT	1 cut(s)	498
CseI	GACGC	1 cut(s)	392
Csp6I	GTAC	4 cut(s)	806, 1001, 1334, 1425
CspCI	CAANNNNNGTGG	2 cut(s)	399, 434
CviQI	GTAC	4 cut(s)	806, 1001, 1334, 1425
DdeI	CTNAG	9 cut(s)	621, 684, 759, 944, 1105, 1269, 1657, 1986, 2087
DpnI	GATC	6 cut(s)	1267, 1275, 1520, 1626, 1639, 1737
DpnII	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
DraIII	CACNNNGTG	1 cut(s)	374
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	1 cut(s)	84
Eam1104I	CTCTTC	1 cut(s)	1326
EarI	CTCTTC	1 cut(s)	1326
Eco130I	CCWWGG	7 cut(s)	21, 81, 410, 575, 846, 872, 1465
Eco147I	AGGCCT	1 cut(s)	354
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	2 cut(s)	585, 1547
Eco57I	CTGAAG	5 cut(s)	128, 492, 770, 974, 1544
Eco72I	CACGTG	1 cut(s)	629
Eco91I	GGTNACC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	1 cut(s)	620
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	1 cut(s)	1789
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	7 cut(s)	21, 81, 410, 575, 846, 872, 1465
ErhI	CCWWGG	7 cut(s)	21, 81, 410, 575, 846, 872, 1465
FalI	AAGNNNNNCTT	3 cut(s)	37, 1350, 1382
FaqI	GGGAC	2 cut(s)	571, 1560
FauI	CCCGC	1 cut(s)	304
FblI	GTMKAC	1 cut(s)	2025
Fnu4HI	GCNGC	7 cut(s)	129, 309, 333, 561, 564, 1899, 2070
FokI	GGATG	5 cut(s)	245, 327, 1213, 1889, 2089
Fsp4HI	GCNGC	7 cut(s)	129, 309, 333, 561, 564, 1899, 2070
FspBI	CTAG	5 cut(s)	350, 648, 1038, 1611, 1767
GlaI	GCGC	2 cut(s)	567, 1471
GluI	GCNGC	7 cut(s)	129, 309, 333, 561, 564, 1899, 2070
GsuI	CTGGAG	1 cut(s)	207
HaeIII	GGCC	5 cut(s)	86, 128, 354, 1536, 1984
HapII	CCGG	3 cut(s)	228, 383, 1948
HgaI	GACGC	1 cut(s)	392
HhaI	GCGC	2 cut(s)	568, 1472
Hin1I	GRCGYC	2 cut(s)	9, 144
Hin6I	GCGC	2 cut(s)	566, 1470
HinP1I	GCGC	2 cut(s)	566, 1470
HincII	GTYRAC	4 cut(s)	76, 262, 274, 366
HindII	GTYRAC	4 cut(s)	76, 262, 274, 366
HindIII	AAGCTT	2 cut(s)	485, 605
HinfI	GANTC	2 cut(s)	287, 1763
HpaII	CCGG	3 cut(s)	228, 383, 1948
HphI	GGTGA	6 cut(s)	10, 46, 1190, 1632, 1864, 2042
Hpy166II	GTNNAC	9 cut(s)	76, 220, 262, 274, 366, 1001, 1181, 2026, 2093
Hpy8I	GTNNAC	9 cut(s)	76, 220, 262, 274, 366, 1001, 1181, 2026, 2093
Hpy99I	CGWCG	3 cut(s)	41, 146, 670
HpyCH4III	ACNGT	7 cut(s)	41, 280, 585, 952, 1381, 1424, 2030
HpyCH4IV	ACGT	3 cut(s)	144, 204, 628
HpyCH4V	TGCA	6 cut(s)	323, 560, 1209, 1436, 1916, 2093
HpyF10VI	GCNNNNNNNGC	6 cut(s)	324, 329, 421, 827, 1070, 1533
HpyF3I	CTNAG	9 cut(s)	621, 684, 759, 944, 1105, 1269, 1657, 1986, 2087
HpySE526I	ACGT	3 cut(s)	144, 204, 628
Hsp92I	GRCGYC	2 cut(s)	9, 144
HspAI	GCGC	2 cut(s)	566, 1470
Kzo9I	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
LmnI	GCTCC	3 cut(s)	340, 617, 676
Lsp1109I	GCAGC	4 cut(s)	295, 319, 572, 1910
MaeI	CTAG	5 cut(s)	350, 648, 1038, 1611, 1767
MaeII	ACGT	3 cut(s)	144, 204, 628
MaeIII	GTNAC	8 cut(s)	16, 160, 579, 850, 1033, 1418, 1810, 2118
MalI	GATC	6 cut(s)	1267, 1275, 1520, 1626, 1639, 1737
MbiI	CCGCTC	1 cut(s)	2069
MboI	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
MfeI	CAATTG	1 cut(s)	435
MflI	RGATCY	1 cut(s)	1265
MhlI	GDGCHC	4 cut(s)	846, 950, 1291, 2095
MlsI	TGGCCA	1 cut(s)	86
MluNI	TGGCCA	1 cut(s)	86
MlyI	GAGTC	1 cut(s)	1772
MmeI	TCCRAC	5 cut(s)	10, 1231, 1363, 1576, 1579
Mox20I	TGGCCA	1 cut(s)	86
MroXI	GAANNNNTTC	2 cut(s)	181, 987
MscI	TGGCCA	1 cut(s)	86
MseI	TTAA	5 cut(s)	639, 768, 867, 1023, 1086
MslI	CAYNNNNRTG	1 cut(s)	56
Msp20I	TGGCCA	1 cut(s)	86
MspA1I	CMGCKG	1 cut(s)	563
MspI	CCGG	3 cut(s)	228, 383, 1948
MspR9I	CCNGG	4 cut(s)	229, 384, 1791, 1949
MunI	CAATTG	1 cut(s)	435
Mva1269I	GAATGC	1 cut(s)	1448
MvaI	CCWGG	1 cut(s)	1791
MvnI	CGCG	2 cut(s)	131, 568
MwoI	GCNNNNNNNGC	6 cut(s)	324, 329, 421, 827, 1070, 1533
NciI	CCSGG	3 cut(s)	229, 384, 1949
NcoI	CCATGG	1 cut(s)	575
NdeII	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
NlaIV	GGNNCC	4 cut(s)	587, 672, 843, 1548
NmuCI	GTSAC	2 cut(s)	16, 2118
NspI	RCATGY	3 cut(s)	812, 1866, 2128
NspV	TTCGAA	1 cut(s)	290
PceI	AGGCCT	1 cut(s)	354
PciI	ACATGT	1 cut(s)	808
PcsI	WCGNNNNNNNCGW	2 cut(s)	45, 1633
PctI	GAATGC	1 cut(s)	1448
PdmI	GAANNNNTTC	2 cut(s)	181, 987
PfeI	GAWTC	1 cut(s)	287
PflFI	GACNNNGTC	1 cut(s)	41
PfoI	TCCNGGA	1 cut(s)	382
PkrI	GCNGC	7 cut(s)	130, 310, 334, 562, 565, 1900, 2071
PleI	GAGTC	1 cut(s)	1771
PmaCI	CACGTG	1 cut(s)	629
PmlI	CACGTG	1 cut(s)	629
PpsI	GAGTC	1 cut(s)	1771
Ppu21I	YACGTR	1 cut(s)	629
PscI	ACATGT	1 cut(s)	808
Psp1406I	AACGTT	1 cut(s)	204
Psp6I	CCWGG	1 cut(s)	1789
PspCI	CACGTG	1 cut(s)	629
PspEI	GGTNACC	1 cut(s)	16
PspGI	CCWGG	1 cut(s)	1789
PspN4I	GGNNCC	4 cut(s)	587, 672, 843, 1548
PspPI	GGNCC	3 cut(s)	585, 1547, 1982
PsrI	GAACNNNNNNTAC	2 cut(s)	945, 977
PsuI	RGATCY	1 cut(s)	1265
PsyI	GACNNNGTC	1 cut(s)	41
RsaI	GTAC	4 cut(s)	807, 1002, 1335, 1426
RsaNI	GTAC	4 cut(s)	806, 1001, 1334, 1425
RseI	CAYNNNNRTG	1 cut(s)	56
SaqAI	TTAA	5 cut(s)	639, 768, 867, 1023, 1086
SatI	GCNGC	7 cut(s)	129, 309, 333, 561, 564, 1899, 2070
Sau3AI	GATC	6 cut(s)	1265, 1273, 1518, 1624, 1637, 1735
Sau96I	GGNCC	3 cut(s)	585, 1547, 1982
SchI	GAGTC	1 cut(s)	1772
ScrFI	CCNGG	4 cut(s)	229, 384, 1791, 1949
SduI	GDGCHC	4 cut(s)	846, 950, 1291, 2095
SfcI	CTRYAG	1 cut(s)	930
SfuI	TTCGAA	1 cut(s)	290
SinI	GGWCC	2 cut(s)	585, 1547
SmiMI	CAYNNNNRTG	1 cut(s)	56
SmlI	CTYRAG	4 cut(s)	98, 516, 1184, 2006
SmoI	CTYRAG	4 cut(s)	98, 516, 1184, 2006
SseBI	AGGCCT	1 cut(s)	354
SsiI	CCGC	5 cut(s)	5, 129, 311, 563, 2069
SspMI	CTAG	5 cut(s)	350, 648, 1038, 1611, 1767
StuI	AGGCCT	1 cut(s)	354
StyD4I	CCNGG	4 cut(s)	227, 382, 1789, 1947
StyI	CCWWGG	7 cut(s)	21, 81, 410, 575, 846, 872, 1465
TaaI	ACNGT	7 cut(s)	41, 280, 585, 952, 1381, 1424, 2030
TaiI	ACGT	3 cut(s)	147, 207, 631
TatI	WGTACW	1 cut(s)	805
TauI	GCSGC	3 cut(s)	131, 566, 2072
TfiI	GAWTC	1 cut(s)	287
Tru1I	TTAA	5 cut(s)	639, 768, 867, 1023, 1086
Tru9I	TTAA	5 cut(s)	639, 768, 867, 1023, 1086
TscAI	CASTG	2 cut(s)	283, 955
TseFI	GTSAC	2 cut(s)	16, 2118
TseI	GCWGC	4 cut(s)	308, 332, 560, 1898
Tsp45I	GTSAC	2 cut(s)	16, 2118
TspDTI	ATGAA	4 cut(s)	581, 716, 1725, 1893
TspGWI	ACGGA	1 cut(s)	1351
TspRI	CASTG	2 cut(s)	283, 955
Tth111I	GACNNNGTC	1 cut(s)	41
VneI	GTGCAC	1 cut(s)	2091
VpaK11BI	GGWCC	2 cut(s)	585, 1547
XagI	CCTNNNNNAGG	1 cut(s)	620
XapI	RAATTY	2 cut(s)	983, 1824
XbaI	TCTAGA	1 cut(s)	1766
XceI	RCATGY	3 cut(s)	812, 1866, 2128
XmiI	GTMKAC	1 cut(s)	2025
XmnI	GAANNNNTTC	2 cut(s)	181, 987
XspI	CTAG	5 cut(s)	350, 648, 1038, 1611, 1767
ZraI	GACGTC	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.