Rroxscaffold_2G00151470
NAC Family

proteinaceous RNase P 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002433
Physical Location & Seq
Reverse (-)
88710859 .. 88722899
12041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151470.1

Sequence Viewer

Length: 4758 bp
ATGGCGGGCGTCAAGGTCACTTTGGAGGTCGGAAGCGACGGCGTCGCCGTCATCACCATGTTCAATCCTCCCGTCAACGCCTTGGCCATCTCCATTCTAAAGGGGCTGAAGGAGAAGTTCGATGAAGCCGCGAGGAGAAACGACGTCAAGGCTATTGTCGTTACTGGAAATGGAGGAAGGTTTTCTGGAGGTTTTGATATCAATGTTTTCGAGAAGGTTCACGCTACCGGGAACGTTTCGCTTATGCCTGATGTATCTGTTGACTTGATGGTCAACACTGTTGAAGATTCGAAGAAGCCTGTTGTTGCTGCGGTGGAAGGACTTGCACTCGGAGGGATGCCATGCACGCATTGCTGCTCAAGAACTCAACTAGGCCTGCCAGAGTTGACACTTGGTGTTATTCCGGGATTTGGAGGCACACAGCGTCTTCCAAGGCTTGCGGGGCTTCCAAAGGCAGTTGAGATGATGTTGTTATCCAAGTCAATCATGTCTGAAGAAGGGGCAAAGCTTGGTCTTATCGATGCTATTGTGCCTTCTCAAGAGTTGCTGAAAGTATCTCGATTATGGGCTTTGGACATTGCAGCGGCGCGAAAACCATGGTTACGGTCCCTTCACAGGACAGACAAGCTTTGCTCCCTATCTGAGGCACGCGAGATCATTAAAGTTGCTAGACAACAAGCCAGAAAGACGGCTCCCAATATGCCTCAGCATCAAGTATGCCTCGATGCTATTGAGGAGGGCATTATTCATGGAGGATATAGTGGAGTTCTGAAGGAAGCTAAGGTTTTTAAGGAGTTAGTTGTATCAGACACTTCAAAAGGTCTTGTACATGTATTTTTTGCCCAACGGGCAACATCAAAGGTGCCCAAGGTTACTGATGTTGGGCTTAAACCAAGGCATATAAAGAAGGTTGCTATCATTGGGGGAGGTCTGATGGGTTCTGGCATAGCTACAGCCCTTCTTCTGAGCAATGTATCTGTTGTTCTCAAAGAAATCAACTCTGAATTTCTTCAGAAGGGTGTACGAACAATAGAAGGAAATGTTAAAGGCTTGGTAACTAGAGGGAAGCTGACACGGGATAAGGCAGACAAAGCTCTCTCATTGCTTAAAGGTAGTCTGGACTACTCAGACTTCAAAGATGTGGATATGGTCATAGAGGCTGTCATTGAGAGCGTTCCTCTCAAACAAAAGATTTTTGGTGAACTTGAGAAAGTTTGCCCTTCTCATTGCATTTTGGCTACAAACACATCCACTATTGACCTCAATGTAGTTGGAGAAAAGACGCGATCTCAGGATCGCATCGTGGGGGCACACTTTTTCAGTCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAATACTTCGGCACAAGTAATTCTTGACCTTTTGACGGTTGGAAAATTAATAAGGAAAGTTCCTGTTGTGGTTGGTGTCGATGTGTTCAGGATTGACAGGATCATCAGCAATTTTGGCCTTCCTATGGGACCATTCCAACTTCAGGATTTGGCAGGCTATGGAGTAGCTCTTGCTGTTGGAAAGATATTTGCTAGTGCTTTCCCTGATCGCACATTTCGATCACCATTGGTCGAACTCTTAGTCAAAAATGGACGAAATGGTAAAAACAATGGAAAAGGATATTACACTTATGAAAAGGGAAGCAAGCCCAAGCCCGATCTGTCTGTGCTACCAATTATTGAGGAGTCTAGACGAATTACCAATATGATGCCTGGTGGAAAGCCTTTATCTGTTACAGACCAAGAAATTCTGGAGATAATACTCTTTCCTGTGGTGAATGAGGCATGTCGTATTTTGGATGAAGGAATAGTTATTCGAGCAGCAGACCTTGACATCGCATCTGTTCTTGGCATGAGTTTCCCAAATTACCGGGGTGGCATTGTCTTTTGGGCAGATATGGTTGGGCCTAAGCATATATACGCTACTCTCAAGAAATGGTCAGGAGTATACGGTGGTTTCTTCAAACCATCAAGGTTTTTAGAAGAAAGAGCAACAAAAGGCTTGCCTCTGAGTGCACCCGCATCATCCTCATCTTCAAGGTCACGCATGTTACCATTGGAACTGAGCAGCGTGGAATCGATGATGCCATTGCACAGTGCAATTGCTTCGGCGCGGTTAACGTCGAGCTTGTCAATTGAGTCCCAGAGCTGGGGATTAGTTCCTCAAGTTAATCCATATCGGTATCGGCCCAGCAAACTCCTCCAACGGCCACACATGGCCTCCTTCACCTCCTCCAACTCAGTACTGCAGCAAAAGCACCACCACCTTCTTCACTCCATCACCACCCTCTGTAAGTACCCATCTCCTCTCAACGTTTTTGAATTCCACTGCCCCTCTCATTTCTTCACTTTCTCACCTCCCAAGCAAACACTTCAACTCAAACACACAGTAAAACTCTATCCACTTCCACTAGCTAGAGAGAATATCAACCACATAGTTGCAAAGTTTTCCACCTCCACCTCCACCACCACGTACCATGAACCACACACAAAGACCCTGAACTCAGCAACTGAAAAACCTGTAAAGAACAATCGCAGTTCAGTTGTAGAGAAAAAGAGTGAAGCTAGGTTCACTAAAGAGAAAAGTACCAAGAAAAGTAGAAGGAAGGAGGGTATGTGGTTTAAAGATGAGAGTAAGGAAAGAAAGAAGCGAGGTAGTGATAGTGATCAGATGGGAGAAGAGAAAAAGGGGAGAGGGTCTAAGAAAAACAAGGTTAATGTAGACTTGCCTGAAGTTAAGATGAGAGTTGGATTGGATATGTGCTCAAAGAGAGGGGATGTAATGGGTGCAATTAAGTACTATGATTTGGCTCAAACTGAGCAGATTAAGTTGGATCAGTATCATTATACTGTTCTGTTGTATCTGTGCTCTTCTGCAGCTGTTGGTGTTGTTCGTCCTGCTAAGAGTGGGTCTGGGACTCGAGTTTTGGATATCGTGGATTCATCTGGCGAAGGAACTGGGGTGGAAACAATGGAGTTGAGTAAGTTAAGAGACTCGGGTAGTGGGAATTGGGATGATAACAGTGGAAGTGATGAGAGTGCTGAAAAAATGGAGTTGAGTTCAAGGAACAGGTTTGATGATGATGTGGATGGTGCTTCCCATGAAAAGAGGAACTTAGCGTGGTTTTCTAATGGATTTGTGAAGCGAAACTCTCGCCTGTTAGATGGACTGAACAATCCTACCAAAGTTGGAGATGATGGTACAAGTCTAAAAGATGGAAATGTGAAGCAAGAAAGCAATGAGATTCGGGTTAGTGAGGATGTGAAGAAGTATGCACTCCAAAGGGGATTTGAGATTTATGAGAAGATGTGTTTGGATGGTGTCCCAGTGAATGAAGCAGCTTTGACATCTGTGGCTAGAATGGCAATGTCAATGGGGGACGGAGACATGGCATTTGACATGGTGAAGCAGATGAAGTCGATGGGAATAAATCCTAGACTGCGTTCCTACGGTCCTGCGTTATCTGCTTTTTGCAGTAGTGGCAATATTGACAAAGCTTTTGCCGTTGAGAAACACATGTTGGAGCATGGTGTCTATCCAGAAGAACCTGAACTGGAGGCCCTCTTAAGAGTGAGTGTGGGAGCTGGAAAAGGTGACAAGGTGTATTATATGTTGCAGAAACTAAGAGCAAGTGTAAGGAGGGTCTCACCCTCTACTGCTGACTTAATTGTAAAGTGGTTTAATAGCAAGGAAGGGTCGAGAGTGGGAAAGACAAAATGGGATGAAAGATTAATAAGGCAGGCCATTGAAAATGGAGGTGGTGGGTGGCATGGGCAGGGCTGGTTGGGGAAAGGAAAGTGGTCAGTGGTACATACAACTATTGGATCTGATGGCTTGTGCAAATGCTGTGGGGGAAAATTGGCCACAATTGATCTTGATCCTGTAGAAACAGAAAACTTTGCTGAATCAGTTGCATCTATAGCTATAAAGAGAGAGAAAAATTCAAGCTTTCAGAAATTTCAAGTACGAAATGTCCTCAACAATGCTCTTGCTTTTAATCTTGTCAATGCTGTCGTTAATGGCATACGCCAGAAGCTTCCTTCGAAGAGATGGCCACTCATTGTTCTTCATAATAGGCGTATCACTGGAGGCAGAATGGATGAACGGGTCAATAAGGCGTTGATTGAGAAGTGGCAAAATGCTGATGCTCTCTATGCAACACCTACTGGGTCAAATGATGATTGGTACTGGTTGTATGCTGCAATTAAATTCAAGTGCTTACTTGTGACAAATGATGAGATGAGAGACCATACGTTTCAACTTCTTGGGAATGATTTCTTTCCGAGATGGAAAGAAAGGCATCAAGTGCACTTCAGTTTTTCTGAAGGTGGTCCAGTGTTTCATATGCCTCCTCCTTGTTCTGTTGTAATTCAGGAATCAGAGGAAGGGCACTGGCACATTCCAGTGGTGTCAGAACATGGTTATGAAGCTGAGAGAATGTGGTTATGCATTATGCGAGCTAAATCACGCGAGGCAAGGAAGGATTCTGCAACCAGACCTGAAGATGCAGAACCTCATAACAAGGGATTTGCTAGGTCAGCTACCAGAACTGGCATTGAGTCACGACCGTTGAAAAGTGCGAACCACAAATATACTAAACACCGACCCCAGGAAATTCTCAAGAATCTCAAAGATATTCTATCAGGTTCCCCAATCTCGAATCATCGTTCCATAATACCAGAAATTGAGACTGCAGAGACGATTGGTGGATGTACCATTGACTTTCAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PPR_long PRORP

Protein Analysis

1585

Amino Acids

175.02

Weight (kDa)

9.3

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 13 - 204 6e-29 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 25 - 192 1.7e-13 Enoyl-CoA hydratase/isomerase
3HCDH_N PF02737 303 - 479 2.8e-57 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH PF00725 481 - 552 1.1e-14 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
PPR_long PF17177 910 - 971 3.2e-13 Pentacotripeptide-repeat region of PRORP
PPR_long PF17177 1096 - 1240 6.8e-52 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 1122 - 1233 1.4e-07 Fungal tetratrico peptide repeats
PRORP PF16953 1282 - 1491 1.9e-61 Protein-only RNase P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AatII GACGTC 1 cut(s) 147
AccB1I GGYRCC 1 cut(s) 862
AccI GTMKAC 2 cut(s) 1971, 2745
AccII CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
AciI CCGC 7 cut(s) 5, 129, 311, 440, 584, 2043, 2137
AclI AACGTT 2 cut(s) 234, 2337
AclWI GGATC 5 cut(s) 1302, 1472, 2865, 3856, 3896
AcoI YGGCCR 4 cut(s) 84, 2231, 3885, 4076
AcsI RAATTY 7 cut(s) 1004, 1770, 2345, 3964, 3980, 4232, 4638
AcuI CTGAAG 9 cut(s) 128, 513, 791, 995, 1490, 2775, 4321, 4368, 4545
AcyI GRCGYC 3 cut(s) 9, 42, 144
AdeI CACNNNGTG 1 cut(s) 395
AfiI CCNNNNNNNGG 9 cut(s) 410, 615, 643, 1399, 1489, 1507, 2172, 2173, 4633
AflII CTTAAG 1 cut(s) 3589
AflIII ACRYGT 2 cut(s) 829, 3540
AjnI CCWGG 2 cut(s) 1735, 4632
AjuI GAANNNNNNNTTGG 4 cut(s) 3320, 3352, 3527, 3559
Alw21I GWGCWC 4 cut(s) 2041, 2789, 2894, 4335
Alw26I GTCTC 6 cut(s) 3009, 3402, 3673, 4263, 4706, 4715
Alw44I GTGCAC 2 cut(s) 2037, 4331
AlwI GGATC 5 cut(s) 1302, 1472, 2865, 3856, 3896
AlwNI CAGNNNCTG 1 cut(s) 2534
Ama87I CYCGRG 2 cut(s) 2943, 3019
ApaLI GTGCAC 2 cut(s) 2037, 4331
ApeKI GCWGC 9 cut(s) 308, 354, 581, 1844, 2091, 2272, 2900, 3362, 4223
ApoI RAATTY 7 cut(s) 1004, 1770, 2345, 3964, 3980, 4232, 4638
ArsI GACNNNNNNTTYG 8 cut(s) 1590, 1622, 2932, 2964, 3401, 3433, 3506, 3538
AseI ATTAAT 2 cut(s) 1412, 3755
Asp700I GAANNNNTTC 3 cut(s) 181, 1008, 4298
AspLEI GCGC 2 cut(s) 589, 2137
AspS9I GGNCC 7 cut(s) 606, 1493, 1928, 2211, 3476, 3583, 4355
AsuC2I CCSGG 3 cut(s) 229, 405, 1895
AsuII TTCGAA 2 cut(s) 290, 4067
AvaI CYCGRG 2 cut(s) 2943, 3019
AvaII GGWCC 4 cut(s) 606, 1493, 3476, 4355
BaeGI GKGCMC 5 cut(s) 867, 1312, 2041, 4335, 4416
BalI TGGCCA 3 cut(s) 86, 3887, 4078
BanI GGYRCC 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 4 cut(s) 2041, 2789, 2894, 4335
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 9 cut(s) 295, 341, 593, 1856, 2103, 2284, 2912, 3374, 4210
BceAI ACGGC 5 cut(s) 32, 55, 705, 2246, 3512
BcgI CGANNNNNNTGC 2 cut(s) 2112, 2146
BciT130I CCWGG 2 cut(s) 1737, 4634
BclI TGATCA 1 cut(s) 2689
BcnI CCSGG 3 cut(s) 229, 405, 1895
BcoDI GTCTC 6 cut(s) 3009, 3402, 3673, 4263, 4706, 4715
BfmI CTRYAG 6 cut(s) 951, 2270, 2898, 3906, 3942, 4716
BfrI CTTAAG 1 cut(s) 3589
BglI GCCNNNNNGGC 1 cut(s) 848
BmcAI AGTACT 2 cut(s) 2268, 2822
Bme1390I CCNGG 5 cut(s) 229, 405, 1737, 1895, 4634
Bme18I GGWCC 4 cut(s) 606, 1493, 3476, 4355
BmeT110I CYCGRG 2 cut(s) 2943, 3019
BmgT120I GGNCC 7 cut(s) 606, 1493, 1928, 2211, 3476, 3583, 4355
BmiI GGNNCC 5 cut(s) 608, 693, 864, 1494, 4672
BmrFI CCNGG 5 cut(s) 229, 405, 1737, 1895, 4634
BmrI ACTGGG 3 cut(s) 2991, 3344, 4200
BmuI ACTGGG 3 cut(s) 2991, 3344, 4200
BpiI GAAGAC 1 cut(s) 419
BplI GAGNNNNNCTC 2 cut(s) 1162, 1194
BpmI CTGGAG 4 cut(s) 207, 1796, 3599, 4131
Bpu10I CCTNAGC 3 cut(s) 705, 780, 1932
Bpu14I TTCGAA 2 cut(s) 290, 4067
BpuEI CTTGAG 6 cut(s) 343, 522, 1226, 1937, 2172, 4628
BpuMI CCSGG 3 cut(s) 229, 405, 1895
Bsa29I ATCGAT 2 cut(s) 519, 2102
BsaAI YACGTR 1 cut(s) 2496
BsaBI GATNNNNATC 3 cut(s) 2688, 2862, 3900
BsaHI GRCGYC 3 cut(s) 9, 42, 144
BsaI GGTCTC 2 cut(s) 3673, 4263
BsaJI CCNNGG 7 cut(s) 81, 431, 596, 867, 893, 1894, 4632
BsaXI ACNNNNNCTCC 4 cut(s) 2228, 2258, 2624, 2654
Bsc4I CCNNNNNNNGG 9 cut(s) 410, 615, 643, 1399, 1489, 1507, 2172, 2173, 4633
Bse3DI GCAATG 8 cut(s) 349, 576, 976, 1100, 1225, 2111, 3268, 3396
Bse8I GATNNNNATC 3 cut(s) 2688, 2862, 3900
BseBI CCWGG 2 cut(s) 1737, 4634
BseCI ATCGAT 2 cut(s) 519, 2102
BseDI CCNNGG 7 cut(s) 81, 431, 596, 867, 893, 1894, 4632
BseJI GATNNNNATC 3 cut(s) 2688, 2862, 3900
BseLI CCNNNNNNNGG 9 cut(s) 410, 615, 643, 1399, 1489, 1507, 2172, 2173, 4633
BseMI GCAATG 8 cut(s) 349, 576, 976, 1100, 1225, 2111, 3268, 3396
BseRI GAGGAG 7 cut(s) 148, 749, 1721, 2213, 2245, 2319, 4365
BseSI GKGCMC 5 cut(s) 867, 1312, 2041, 4335, 4416
BseXI GCAGC 9 cut(s) 295, 341, 593, 1856, 2103, 2284, 2912, 3374, 4210
BseYI CCCAGC 2 cut(s) 2172, 2213
Bsh1236I CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
Bsh1285I CGRYCG 1 cut(s) 4592
BshNI GGYRCC 1 cut(s) 862
BshVI ATCGAT 2 cut(s) 519, 2102
BsiEI CGRYCG 1 cut(s) 4592
BsiHKAI GWGCWC 4 cut(s) 2041, 2789, 2894, 4335
BsiHKCI CYCGRG 2 cut(s) 2943, 3019
BsiSI CCGG 3 cut(s) 228, 404, 1894
BslFI GGGAC 6 cut(s) 592, 1506, 2149, 2953, 3332, 3416
BslI CCNNNNNNNGG 9 cut(s) 410, 615, 643, 1399, 1489, 1507, 2172, 2173, 4633
BsmAI GTCTC 6 cut(s) 3009, 3402, 3673, 4263, 4706, 4715
BsmBI CGTCTC 1 cut(s) 4715
BsmFI GGGAC 6 cut(s) 592, 1506, 2149, 2953, 3332, 3416
Bso31I GGTCTC 2 cut(s) 3673, 4263
BsoBI CYCGRG 2 cut(s) 2943, 3019
Bsp119I TTCGAA 2 cut(s) 290, 4067
Bsp1286I GDGCHC 7 cut(s) 867, 1312, 2041, 2789, 2894, 4335, 4416
Bsp1407I TGTACA 1 cut(s) 826
Bsp19I CCATGG 1 cut(s) 596
BspACI CCGC 7 cut(s) 5, 129, 311, 440, 584, 2043, 2137
BspDI ATCGAT 2 cut(s) 519, 2102
BspFNI CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
BspLI GGNNCC 5 cut(s) 608, 693, 864, 1494, 4672
BspMAI CTGCAG 3 cut(s) 2274, 2902, 4720
BspPI GGATC 5 cut(s) 1302, 1472, 2865, 3856, 3896
BspQI GCTCTTC 1 cut(s) 2899
BspT104I TTCGAA 2 cut(s) 290, 4067
BspT107I GGYRCC 1 cut(s) 862
BspTI CTTAAG 1 cut(s) 3589
BspTNI GGTCTC 2 cut(s) 3673, 4263
BsrDI GCAATG 8 cut(s) 349, 576, 976, 1100, 1225, 2111, 3268, 3396
BsrGI TGTACA 1 cut(s) 826
BssECI CCNNGG 7 cut(s) 81, 431, 596, 867, 893, 1894, 4632
BssNAI GTATAC 1 cut(s) 1972
BssNI GRCGYC 3 cut(s) 9, 42, 144
BssT1I CCWWGG 5 cut(s) 81, 431, 596, 867, 893
Bst1107I GTATAC 1 cut(s) 1972
Bst2UI CCWGG 2 cut(s) 1737, 4634
Bst6I CTCTTC 4 cut(s) 1347, 2697, 2899, 4064
BstACI GRCGYC 3 cut(s) 9, 42, 144
BstAFI CTTAAG 1 cut(s) 3589
BstAPI GCANNNNNTGC 2 cut(s) 351, 4330
BstAUI TGTACA 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 2496
BstBI TTCGAA 2 cut(s) 290, 4067
BstDSI CCRYGG 1 cut(s) 596
BstENI CCTNNNNNAGG 1 cut(s) 641
BstFNI CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
BstHHI GCGC 2 cut(s) 589, 2137
BstMAI GTCTC 6 cut(s) 3009, 3402, 3673, 4263, 4706, 4715
BstMCI CGRYCG 1 cut(s) 4592
BstNI CCWGG 2 cut(s) 1737, 4634
BstNSI RCATGY 4 cut(s) 833, 1812, 2074, 3544
BstSCI CCNGG 5 cut(s) 227, 403, 1735, 1893, 4632
BstSFI CTRYAG 6 cut(s) 951, 2270, 2898, 3906, 3942, 4716
BstSLI GKGCMC 5 cut(s) 867, 1312, 2041, 4335, 4416
BstUI CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
BstV1I GCAGC 9 cut(s) 295, 341, 593, 1856, 2103, 2284, 2912, 3374, 4210
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 3848
BstYI RGATCY 1 cut(s) 3848
BstZ17I GTATAC 1 cut(s) 1972
Bsu15I ATCGAT 2 cut(s) 519, 2102
BsuTUI ATCGAT 2 cut(s) 519, 2102
BtgI CCRYGG 1 cut(s) 596
BtgZI GCGATG 1 cut(s) 1843
BtsI GCAGTG 1 cut(s) 2350
CaiI CAGNNNCTG 1 cut(s) 2534
CfoI GCGC 2 cut(s) 589, 2137
Cfr13I GGNCC 7 cut(s) 606, 1493, 1928, 2211, 3476, 3583, 4355
ClaI ATCGAT 2 cut(s) 519, 2102
CseI GACGC 3 cut(s) 31, 413, 1291
CspCI CAANNNNNGTGG 2 cut(s) 3853, 3888
DraI TTTAAA 1 cut(s) 2647
DraIII CACNNNGTG 1 cut(s) 395
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 4 cut(s) 84, 2231, 3885, 4076
Eam1104I CTCTTC 4 cut(s) 1347, 2697, 2899, 4064
EarI CTCTTC 4 cut(s) 1347, 2697, 2899, 4064
Eco130I CCWWGG 5 cut(s) 81, 431, 596, 867, 893
Eco147I AGGCCT 1 cut(s) 375
Eco31I GGTCTC 2 cut(s) 3673, 4263
Eco32I GATATC 2 cut(s) 199, 2956
Eco47I GGWCC 4 cut(s) 606, 1493, 3476, 4355
Eco57I CTGAAG 9 cut(s) 128, 513, 791, 995, 1490, 2775, 4321, 4368, 4545
Eco88I CYCGRG 2 cut(s) 2943, 3019
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 3583
EcoRI GAATTC 1 cut(s) 2345
EcoRII CCWGG 2 cut(s) 1735, 4632
EcoRV GATATC 2 cut(s) 199, 2956
EcoT14I CCWWGG 5 cut(s) 81, 431, 596, 867, 893
EcoT22I ATGCAT 1 cut(s) 4475
ErhI CCWWGG 5 cut(s) 81, 431, 596, 867, 893
Esp3I CGTCTC 1 cut(s) 4715
FalI AAGNNNNNCTT 4 cut(s) 1371, 1403, 3122, 3154
FaqI GGGAC 6 cut(s) 592, 1506, 2149, 2953, 3332, 3416
FauI CCCGC 2 cut(s) 433, 2050
FauNDI CATATG 1 cut(s) 4368
FbaI TGATCA 1 cut(s) 2689
FblI GTMKAC 2 cut(s) 1971, 2745
GlaI GCGC 2 cut(s) 588, 2136
GsaI CCCAGC 2 cut(s) 2176, 2217
GsuI CTGGAG 4 cut(s) 207, 1796, 3599, 4131
HapII CCGG 3 cut(s) 228, 404, 1894
HgaI GACGC 3 cut(s) 31, 413, 1291
HhaI GCGC 2 cut(s) 589, 2137
Hin1I GRCGYC 3 cut(s) 9, 42, 144
Hin6I GCGC 2 cut(s) 587, 2135
HinP1I GCGC 2 cut(s) 587, 2135
HincII GTYRAC 5 cut(s) 76, 262, 274, 387, 2142
HindII GTYRAC 5 cut(s) 76, 262, 274, 387, 2142
HindIII AAGCTT 5 cut(s) 506, 626, 3519, 3970, 4058
HpaI GTTAAC 1 cut(s) 2142
HpaII CCGG 3 cut(s) 228, 404, 1894
Hpy99I CGWCG 4 cut(s) 41, 47, 146, 2149
HpyCH4IV ACGT 6 cut(s) 144, 234, 2144, 2337, 2495, 4277
HpySE526I ACGT 6 cut(s) 144, 234, 2144, 2337, 2495, 4277
Hsp92I GRCGYC 3 cut(s) 9, 42, 144
HspAI GCGC 2 cut(s) 587, 2135
Ksp22I TGATCA 1 cut(s) 2689
KspAI GTTAAC 1 cut(s) 2142
LguI GCTCTTC 1 cut(s) 2899
LmnI GCTCC 4 cut(s) 638, 697, 3547, 3605
Lsp1109I GCAGC 9 cut(s) 295, 341, 593, 1856, 2103, 2284, 2912, 3374, 4210
MaeII ACGT 6 cut(s) 144, 234, 2144, 2337, 2495, 4277
MfeI CAATTG 3 cut(s) 2124, 2157, 3891
MflI RGATCY 1 cut(s) 3848
MhlI GDGCHC 7 cut(s) 867, 1312, 2041, 2789, 2894, 4335, 4416
MlsI TGGCCA 3 cut(s) 86, 3887, 4078
MluNI TGGCCA 3 cut(s) 86, 3887, 4078
MlyI GAGTC 5 cut(s) 1718, 2171, 2935, 3011, 4592
Mox20I TGGCCA 3 cut(s) 86, 3887, 4078
Mph1103I ATGCAT 1 cut(s) 4475
MroXI GAANNNNTTC 3 cut(s) 181, 1008, 4298
MscI TGGCCA 3 cut(s) 86, 3887, 4078
MslI CAYNNNNRTG 3 cut(s) 56, 4427, 4446
Msp20I TGGCCA 3 cut(s) 86, 3887, 4078
MspA1I CMGCKG 2 cut(s) 584, 2903
MspCI CTTAAG 1 cut(s) 3589
MspI CCGG 3 cut(s) 228, 404, 1894
MspR9I CCNGG 5 cut(s) 229, 405, 1737, 1895, 4634
MunI CAATTG 3 cut(s) 2124, 2157, 3891
MvaI CCWGG 2 cut(s) 1737, 4634
MvnI CGCG 6 cut(s) 131, 589, 651, 1285, 2137, 4494
NciI CCSGG 3 cut(s) 229, 405, 1895
NcoI CCATGG 1 cut(s) 596
NdeI CATATG 1 cut(s) 4368
NlaIV GGNNCC 5 cut(s) 608, 693, 864, 1494, 4672
NmuCI GTSAC 5 cut(s) 16, 2064, 3617, 4249, 4584
NsiI ATGCAT 1 cut(s) 4475
NspI RCATGY 4 cut(s) 833, 1812, 2074, 3544
NspV TTCGAA 2 cut(s) 290, 4067
PaeR7I CTCGAG 1 cut(s) 2943
PceI AGGCCT 1 cut(s) 375
PciI ACATGT 2 cut(s) 829, 3540
PciSI GCTCTTC 1 cut(s) 2899
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 1579
PdmI GAANNNNTTC 3 cut(s) 181, 1008, 4298
PfeI GAWTC 9 cut(s) 287, 2099, 2963, 3268, 3929, 4400, 4508, 4648, 4684
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 403
PleI GAGTC 5 cut(s) 1717, 2170, 2935, 3011, 4591
PpsI GAGTC 5 cut(s) 1717, 2170, 2935, 3011, 4591
Ppu21I YACGTR 1 cut(s) 2496
PscI ACATGT 2 cut(s) 829, 3540
PshBI ATTAAT 2 cut(s) 1412, 3755
Psp1406I AACGTT 2 cut(s) 234, 2337
Psp6I CCWGG 2 cut(s) 1735, 4632
PspFI CCCAGC 2 cut(s) 2172, 2213
PspGI CCWGG 2 cut(s) 1735, 4632
PspN4I GGNNCC 5 cut(s) 608, 693, 864, 1494, 4672
PspPI GGNCC 7 cut(s) 606, 1493, 1928, 2211, 3476, 3583, 4355
PspXI VCTCGAGB 1 cut(s) 2943
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PstI CTGCAG 3 cut(s) 2274, 2902, 4720
PstNI CAGNNNCTG 1 cut(s) 2534
PsuI RGATCY 1 cut(s) 3848
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 2903
RseI CAYNNNNRTG 3 cut(s) 56, 4427, 4446
SapI GCTCTTC 1 cut(s) 2899
Sau96I GGNCC 7 cut(s) 606, 1493, 1928, 2211, 3476, 3583, 4355
ScaI AGTACT 2 cut(s) 2268, 2822
SchI GAGTC 5 cut(s) 1718, 2171, 2935, 3011, 4592
ScrFI CCNGG 5 cut(s) 229, 405, 1737, 1895, 4634
SduI GDGCHC 7 cut(s) 867, 1312, 2041, 2789, 2894, 4335, 4416
SfcI CTRYAG 6 cut(s) 951, 2270, 2898, 3906, 3942, 4716
Sfr274I CTCGAG 1 cut(s) 2943
SfuI TTCGAA 2 cut(s) 290, 4067
SinI GGWCC 4 cut(s) 606, 1493, 3476, 4355
SlaI CTCGAG 1 cut(s) 2943
SmiMI CAYNNNNRTG 3 cut(s) 56, 4427, 4446
SmlI CTYRAG 8 cut(s) 358, 537, 1205, 1952, 2187, 2943, 3589, 4643
SmoI CTYRAG 8 cut(s) 358, 537, 1205, 1952, 2187, 2943, 3589, 4643
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 7 cut(s) 5, 129, 311, 440, 584, 2043, 2137
SspI AATATT 1 cut(s) 3511
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 5 cut(s) 227, 403, 1735, 1893, 4632
StyI CCWWGG 5 cut(s) 81, 431, 596, 867, 893
TaiI ACGT 6 cut(s) 147, 237, 2147, 2340, 2498, 4280
TatI WGTACW 3 cut(s) 826, 2266, 2820
TauI GCSGC 2 cut(s) 131, 587
TfiI GAWTC 9 cut(s) 287, 2099, 2963, 3268, 3929, 4400, 4508, 4648, 4684
TseFI GTSAC 5 cut(s) 16, 2064, 3617, 4249, 4584
TseI GCWGC 9 cut(s) 308, 354, 581, 1844, 2091, 2272, 2900, 3362, 4223
Tsp45I GTSAC 5 cut(s) 16, 2064, 3617, 4249, 4584
TspGWI ACGGA 2 cut(s) 1372, 3420
Tth111I GACNNNGTC 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 3589
VneI GTGCAC 2 cut(s) 2037, 4331
VpaK11BI GGWCC 4 cut(s) 606, 1493, 3476, 4355
VspI ATTAAT 2 cut(s) 1412, 3755
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 7 cut(s) 1004, 1770, 2345, 3964, 3980, 4232, 4638
XbaI TCTAGA 1 cut(s) 1712
XceI RCATGY 4 cut(s) 833, 1812, 2074, 3544
XhoI CTCGAG 1 cut(s) 2943
XmiI GTMKAC 2 cut(s) 1971, 2745
XmnI GAANNNNTTC 3 cut(s) 181, 1008, 4298
ZraI GACGTC 1 cut(s) 145
ZrmI AGTACT 2 cut(s) 2268, 2822
Zsp2I ATGCAT 1 cut(s) 4475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.