Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

3546721 .. 3552883

Gene Structure 6163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1043300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGCGAAGGTCAAGGTCACCTTGGAGGTCGGCAACGACGGCGTTGCAGTCATCACCATCTTCAATCCACCTGTCAACGCCTTGGCCATACCGACTGTAGCTAGCTTGAAGGAGAAATTCGAGGAGGCCGCGAGGCGAAACGACGTCAAGGCGATCGTTTTGACTGGCAGTGGAGGAAGGTTTTCAGCCGGTTTCGATATCAATGTTTTCGAGAAGGTTCACGAGTCTGGGGATGTTTTGGTTATGCCCGATGTATCTTTTGACTTTGTGCTCAACACACTTGAAGGTTCGAAAAAGCCTGTTGTTGCTGCCATCGAAGGACTTGCACTAGGAGGTGGCTTGGAGTTGGCATTGGGGTGCCATGCACGTATTGCTGCTCCAAGAACTCAACTTGGTCTTCCAGAGTTGTCTCTTGGAGTGATTCCTGGATTAGGAGGCACACAACGTCTTCCAAGGCTTGCAGGGCTTCAGAAGGCAATTGAAATGATGCTGTCATCGAAGCCAATCCTGTCTGAAGAAGGGAAGAAGCTTGGTCTTATAGATGCTATTGCATCTCCTCAAGAGTTGCTGAAAGTATCTAGTTCATGGGCTTTGGAAATTGCAGACAGACGCAAACCATGGTTGCGCTCTCTTCACAGGACAGATAAACTTGCTTCCCTGTCTGAGGCTCGAGAGATACTGAAAATTGCCAGACAACAAGCCAGGAAGACTGCTCCCAATATGCCTCATCACCAAGCATGCCTCAATGTGATCGAGGATGGCATTGTTCATGGAGGATACAGTGGCCTTTTAATGGAGGCTAAAGTTTCTAAGGAGTTACTTCTAGCAGACACTGCAAAAGGTCTTGTCCATGTTTTTTTTGCCCAACGCGCAACATCAAAGGTGCCCAATGTTACTGATGTTGGCCTTAAACCAAGGCAAATAAATAAAGTTGCTATTATTGGGGGAGGTCTGATGGGTTCTGGCATTGCTACAGCCCTTCTCCGCAGCAATATATCTGTGGTTCTAAAGGAAGTCAACTCTGAATATCTTCAGAAGGGCATAGAAAAGATAGAAGGAAACGTTGAAGGCTTAGTAACTAGAAGGGGGCTGACACAGGACAAGGCACAAAAAGCTCTCACATTGCTTAAAGGTAGTTTGGACTACTCTGACTTCAAAGATGTGGATATGGTCATAGAGGCCGTCATTGAAAGTGTTCCTCTCAAACAAAAAATCTTTAGTGAACTCGAGAAAGTTTGTCCTCCTCACTGCGTATTGGCTACAAACACATCCACTATTGACCTCAATGTTGTTGGAGAAGAGACAAACTCTCATGATCGCATTGTGGGGGCACATTTTTTCAGTCCTGCTCATTTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCAGCACAAGTTGTTCTTGATCTTTTTACTGTTGGAAAAATAATAAAGAAAGTTCCTGTTGTGGTTGGTAACTGCACAGGCTTTGCCGTCAATCGAGCATTCTTTCCCTACTCACAAAGTGCCCATTTATTGGTCAATTTAGGAGTGGATGTGTTCAGAATTGACAGGATCATCAGGGATTTTGGCCTACCTATGGGTCCATTCCAGCTTCAAGACTTGGGAGGCTACGGAGTAGCCCTTGCTGCTGGAAAGGAATTATCTCATGCTTTCCCTGATCGCACATTTAAATCTCCGTTGGTTGAGATCTTAATAAAAAATGGACGCAATGGTAAAAACAACGGAAAAGGATATTACGTTTATGAGAAGGGAAGCAAGCCAAAACCTGATCTTTCTGTCCTACCACTTATTGAGGAATCTAGACGAGTTTCCAATATCATGCCTGATGGAAAGCCTTTGACTGTTACAGACCAAGAAATTCTGGAGATGATGCTGTTTCCTGCGGTGAATGAGGCATGTCGTATTCTAGATGAAGGAATAGTTATTCGAGCATCAGACCTTGACATCGCATCTGTTCTTGGGATGAGTTTCCCCTCTTACCGGGGCGGTATTGTGTTCTGGGCAGACCTCGTTGGCCCAAAACACATATACGCTAGTCTCAAGAAATGGTCCGGAACATATGGCAACTTTTTCAAACCATCAAGGTTTTTGGAAGAAAGAGCAACGAGAGGGTTGCCTCTGAGTGCACCAGTGTCGTCGTCCCCGTCTTCAAGGTCACGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

725

Amino Acids

78.4

Weight (kDa)

9.09

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	12 - 212	7.2e-39	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 195	1.5e-22	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	310 - 488	5.1e-58	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	491 - 584	7.8e-20	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	147
AccB1I	GGYRCC	2 cut(s)	357, 883
AccB7I	CCANNNNNTGG	1 cut(s)	1522
AccII	CGCG	2 cut(s)	131, 870
AccIII	TCCGGA	1 cut(s)	2060
AciI	CCGC	4 cut(s)	129, 985, 1892, 1995
AclI	AACGTT	1 cut(s)	1062
AclWI	GGATC	1 cut(s)	1568
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	2 cut(s)	116, 1866
AcuI	CTGAAG	4 cut(s)	452, 534, 1016, 1377
AcyI	GRCGYC	1 cut(s)	144
AdeI	CACNNNGTG	1 cut(s)	1511
AfaI	GTAC	1 cut(s)	1377
AfiI	CCNNNNNNNGG	6 cut(s)	431, 664, 793, 1522, 1585, 1989
AjnI	CCWGG	2 cut(s)	424, 701
AluBI	AGCT	5 cut(s)	101, 105, 529, 1115, 1600
AluI	AGCT	5 cut(s)	101, 105, 529, 1115, 1600
Alw21I	GWGCWC	2 cut(s)	273, 2137
Alw26I	GTCTC	3 cut(s)	414, 1295, 2051
Alw44I	GTGCAC	1 cut(s)	2133
AlwI	GGATC	1 cut(s)	1568
AlwNI	CAGNNNCTG	1 cut(s)	833
Ama87I	CYCGRG	2 cut(s)	669, 1226
Aor13HI	TCCGGA	1 cut(s)	2060
AoxI	GGCC	7 cut(s)	84, 126, 784, 904, 1179, 1576, 2023
ApaLI	GTGCAC	1 cut(s)	2133
ApeKI	GCWGC	4 cut(s)	308, 374, 987, 1634
ApoI	RAATTY	2 cut(s)	116, 1866
Asp700I	GAANNNNTTC	3 cut(s)	181, 1029, 1194
AspLEI	GCGC	2 cut(s)	627, 872
AspS9I	GGNCC	3 cut(s)	1589, 2024, 2058
AsuC2I	CCSGG	1 cut(s)	1991
AsuHPI	GGTGA	4 cut(s)	10, 46, 722, 1906
AsuII	TTCGAA	1 cut(s)	290
AsuNHI	GCTAGC	1 cut(s)	101
AvaI	CYCGRG	2 cut(s)	669, 1226
AvaII	GGWCC	2 cut(s)	1589, 2058
BaeGI	GKGCMC	4 cut(s)	888, 1333, 1516, 2137
BalI	TGGCCA	1 cut(s)	86
BanI	GGYRCC	2 cut(s)	357, 883
BauI	CACGAG	1 cut(s)	221
BbsI	GAAGAC	5 cut(s)	389, 440, 713, 1394, 2148
Bbv12I	GWGCWC	2 cut(s)	273, 2137
BbvI	GCAGC	4 cut(s)	295, 361, 999, 1621
BccI	CCATC	6 cut(s)	65, 320, 752, 949, 1829, 2095
BceAI	ACGGC	3 cut(s)	55, 1166, 1463
BcgI	CGANNNNNNTGC	8 cut(s)	26, 60, 305, 339, 2104, 2124, 2138, 2158
BciT130I	CCWGG	2 cut(s)	426, 703
BciVI	GTATCC	1 cut(s)	770
BcnI	CCSGG	1 cut(s)	1991
BcoDI	GTCTC	3 cut(s)	414, 1295, 2051
BfaI	CTAG	8 cut(s)	102, 329, 579, 824, 1080, 1809, 1916, 2043
BfmI	CTRYAG	2 cut(s)	96, 972
BfuI	GTATCC	1 cut(s)	770
BglII	AGATCT	1 cut(s)	1695
BisI	GCNGC	5 cut(s)	129, 309, 375, 988, 1635
BlsI	GCNGC	5 cut(s)	130, 310, 376, 989, 1636
Bme1390I	CCNGG	3 cut(s)	426, 703, 1991
Bme18I	GGWCC	2 cut(s)	1589, 2058
BmeT110I	CYCGRG	2 cut(s)	669, 1226
BmgT120I	GGNCC	3 cut(s)	1589, 2024, 2058
BmiI	GGNNCC	3 cut(s)	359, 885, 1590
BmrFI	CCNGG	3 cut(s)	426, 703, 1991
BmsI	GCATC	7 cut(s)	477, 532, 560, 1347, 1869, 1949, 1967
BmtI	GCTAGC	1 cut(s)	105
BpiI	GAAGAC	5 cut(s)	389, 440, 713, 1394, 2148
BplI	GAGNNNNNCTC	2 cut(s)	1292, 1324
BpmI	CTGGAG	1 cut(s)	1892
Bpu14I	TTCGAA	1 cut(s)	290
BpuEI	CTTGAG	2 cut(s)	543, 2033
BpuMI	CCSGG	1 cut(s)	1991
BsaAI	YACGTR	1 cut(s)	368
BsaHI	GRCGYC	1 cut(s)	144
BsaJI	CCNNGG	6 cut(s)	21, 81, 452, 617, 914, 1990
BsaWI	WCCGGW	1 cut(s)	2060
BsaXI	ACNNNNNCTCC	2 cut(s)	1527, 1557
Bsc4I	CCNNNNNNNGG	6 cut(s)	431, 664, 793, 1522, 1585, 1989
Bse118I	RCCGGY	1 cut(s)	188
Bse1I	ACTGG	2 cut(s)	169, 2138
Bse3DI	GCAATG	3 cut(s)	966, 1121, 1723
BseAI	TCCGGA	1 cut(s)	2060
BseBI	CCWGG	2 cut(s)	426, 703
BseDI	CCNNGG	6 cut(s)	21, 81, 452, 617, 914, 1990
BseGI	GGATG	5 cut(s)	238, 763, 1268, 1546, 1977
BseLI	CCNNNNNNNGG	6 cut(s)	431, 664, 793, 1522, 1585, 1989
BseMI	GCAATG	3 cut(s)	966, 1121, 1723
BseMII	CTCAG	2 cut(s)	654, 2120
BseNI	ACTGG	2 cut(s)	169, 2138
BseRI	GAGGAG	3 cut(s)	137, 546, 1233
BseSI	GKGCMC	4 cut(s)	888, 1333, 1516, 2137
BseXI	GCAGC	4 cut(s)	295, 361, 999, 1621
BsgI	GTGCAG	1 cut(s)	1450
Bsh1236I	CGCG	2 cut(s)	131, 870
Bsh1285I	CGRYCG	1 cut(s)	156
BshFI	GGCC	7 cut(s)	86, 128, 786, 906, 1181, 1578, 2025
BshNI	GGYRCC	2 cut(s)	357, 883
BsiEI	CGRYCG	1 cut(s)	156
BsiHKAI	GWGCWC	2 cut(s)	273, 2137
BsiHKCI	CYCGRG	2 cut(s)	669, 1226
BsiSI	CCGG	3 cut(s)	189, 1990, 2061
BslFI	GGGAC	1 cut(s)	2134
BslI	CCNNNNNNNGG	6 cut(s)	431, 664, 793, 1522, 1585, 1989
BsmAI	GTCTC	3 cut(s)	414, 1295, 2051
BsmFI	GGGAC	1 cut(s)	2134
BsmI	GAATGC	1 cut(s)	1490
BsnI	GGCC	7 cut(s)	86, 128, 786, 906, 1181, 1578, 2025
BsoBI	CYCGRG	2 cut(s)	669, 1226
Bsp119I	TTCGAA	1 cut(s)	290
Bsp1286I	GDGCHC	5 cut(s)	273, 888, 1333, 1516, 2137
Bsp13I	TCCGGA	1 cut(s)	2060
Bsp143I	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
Bsp19I	CCATGG	1 cut(s)	617
BspACI	CCGC	4 cut(s)	129, 985, 1892, 1995
BspANI	GGCC	7 cut(s)	86, 128, 786, 906, 1181, 1578, 2025
BspCNI	CTCAG	2 cut(s)	655, 2121
BspEI	TCCGGA	1 cut(s)	2060
BspFNI	CGCG	2 cut(s)	131, 870
BspHI	TCATGA	1 cut(s)	1312
BspLI	GGNNCC	3 cut(s)	359, 885, 1590
BspOI	GCTAGC	1 cut(s)	105
BspPI	GGATC	1 cut(s)	1568
BspT104I	TTCGAA	1 cut(s)	290
BspT107I	GGYRCC	2 cut(s)	357, 883
BsrDI	GCAATG	3 cut(s)	966, 1121, 1723
BsrFI	RCCGGY	1 cut(s)	188
BsrI	ACTGG	2 cut(s)	169, 2138
BssAI	RCCGGY	1 cut(s)	188
BssECI	CCNNGG	6 cut(s)	21, 81, 452, 617, 914, 1990
BssMI	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	1 cut(s)	221
BssT1I	CCWWGG	5 cut(s)	21, 81, 452, 617, 914
Bst2BI	CACGAG	1 cut(s)	221
Bst2UI	CCWGG	2 cut(s)	426, 703
Bst4CI	ACNGT	4 cut(s)	97, 782, 1423, 1852
Bst6I	CTCTTC	3 cut(s)	636, 1293, 1368
BstACI	GRCGYC	1 cut(s)	144
BstAPI	GCANNNNNTGC	2 cut(s)	371, 833
BstBAI	YACGTR	1 cut(s)	368
BstBI	TTCGAA	1 cut(s)	290
BstC8I	GCNNGC	4 cut(s)	103, 459, 739, 1766
BstDEI	CTNAG	4 cut(s)	663, 810, 1072, 2129
BstDSI	CCRYGG	1 cut(s)	617
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	2 cut(s)	429, 662
BstF5I	GGATG	5 cut(s)	238, 763, 1268, 1546, 1977
BstFNI	CGCG	2 cut(s)	131, 870
BstHHI	GCGC	2 cut(s)	627, 872
BstKTI	GATC	8 cut(s)	156, 753, 1318, 1414, 1563, 1669, 1698, 1780
BstMAI	GTCTC	3 cut(s)	414, 1295, 2051
BstMBI	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
BstMCI	CGRYCG	1 cut(s)	156
BstMWI	GCNNNNNNNGC	7 cut(s)	39, 371, 463, 833, 869, 1112, 1634
BstNI	CCWGG	2 cut(s)	426, 703
BstNSI	RCATGY	3 cut(s)	741, 1908, 2173
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	3 cut(s)	424, 701, 1989
BstSFI	CTRYAG	2 cut(s)	96, 972
BstSLI	GKGCMC	4 cut(s)	888, 1333, 1516, 2137
BstUI	CGCG	2 cut(s)	131, 870
BstV1I	GCAGC	4 cut(s)	295, 361, 999, 1621
BstV2I	GAAGAC	5 cut(s)	389, 440, 713, 1394, 2148
BstX2I	RGATCY	1 cut(s)	1695
BstYI	RGATCY	1 cut(s)	1695
BsuI	GTATCC	1 cut(s)	770
BsuRI	GGCC	7 cut(s)	86, 128, 786, 906, 1181, 1578, 2025
BtgI	CCRYGG	1 cut(s)	617
BtgZI	GCGATG	1 cut(s)	1939
BtsCI	GGATG	5 cut(s)	238, 763, 1268, 1546, 1977
BtsI	GCAGTG	3 cut(s)	175, 831, 1246
BtsIMutI	CAGTG	5 cut(s)	175, 787, 831, 1246, 2145
Cac8I	GCNNGC	4 cut(s)	103, 459, 739, 1766
CaiI	CAGNNNCTG	1 cut(s)	833
CciI	TCATGA	1 cut(s)	1312
CfoI	GCGC	2 cut(s)	627, 872
Cfr10I	RCCGGY	1 cut(s)	188
Cfr13I	GGNCC	3 cut(s)	1589, 2024, 2058
CseI	GACGC	2 cut(s)	618, 1722
Csp6I	GTAC	1 cut(s)	1376
CviQI	GTAC	1 cut(s)	1376
DdeI	CTNAG	4 cut(s)	663, 810, 1072, 2129
DpnI	GATC	8 cut(s)	155, 752, 1317, 1413, 1562, 1668, 1697, 1779
DpnII	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
DraI	TTTAAA	1 cut(s)	1678
DraIII	CACNNNGTG	1 cut(s)	1511
EaeI	YGGCCR	1 cut(s)	84
Eam1104I	CTCTTC	3 cut(s)	636, 1293, 1368
EarI	CTCTTC	3 cut(s)	636, 1293, 1368
Eco130I	CCWWGG	5 cut(s)	21, 81, 452, 617, 914
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	2 cut(s)	1589, 2058
Eco57I	CTGAAG	4 cut(s)	452, 534, 1016, 1377
Eco88I	CYCGRG	2 cut(s)	669, 1226
Eco91I	GGTNACC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	2 cut(s)	429, 662
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	2 cut(s)	424, 701
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	5 cut(s)	21, 81, 452, 617, 914
ErhI	CCWWGG	5 cut(s)	21, 81, 452, 617, 914
FalI	AAGNNNNNCTT	3 cut(s)	37, 1392, 1424
FaqI	GGGAC	1 cut(s)	2134
FauNDI	CATATG	1 cut(s)	2068
Fnu4HI	GCNGC	5 cut(s)	129, 309, 375, 988, 1635
FokI	GGATG	5 cut(s)	245, 770, 1255, 1553, 1984
Fsp4HI	GCNGC	5 cut(s)	129, 309, 375, 988, 1635
FspBI	CTAG	8 cut(s)	102, 329, 579, 824, 1080, 1809, 1916, 2043
GlaI	GCGC	2 cut(s)	626, 871
GluI	GCNGC	5 cut(s)	129, 309, 375, 988, 1635
GsuI	CTGGAG	1 cut(s)	1892
HaeIII	GGCC	7 cut(s)	86, 128, 786, 906, 1181, 1578, 2025
HapII	CCGG	3 cut(s)	189, 1990, 2061
HgaI	GACGC	2 cut(s)	618, 1722
HhaI	GCGC	2 cut(s)	627, 872
Hin1I	GRCGYC	1 cut(s)	144
Hin6I	GCGC	2 cut(s)	625, 870
HinP1I	GCGC	2 cut(s)	625, 870
HincII	GTYRAC	2 cut(s)	76, 1018
HindII	GTYRAC	2 cut(s)	76, 1018
HindIII	AAGCTT	1 cut(s)	527
HinfI	GANTC	3 cut(s)	224, 421, 1805
HpaII	CCGG	3 cut(s)	189, 1990, 2061
HphI	GGTGA	4 cut(s)	10, 46, 722, 1906
Hpy166II	GTNNAC	5 cut(s)	76, 220, 1018, 1223, 2135
Hpy8I	GTNNAC	5 cut(s)	76, 220, 1018, 1223, 2135
Hpy99I	CGWCG	3 cut(s)	41, 146, 2149
HpyCH4III	ACNGT	4 cut(s)	97, 782, 1423, 1852
HpyCH4IV	ACGT	5 cut(s)	144, 367, 445, 1062, 1746
HpyCH4V	TGCA	9 cut(s)	47, 326, 365, 461, 551, 602, 836, 1467, 2135
HpyF10VI	GCNNNNNNNGC	7 cut(s)	39, 371, 463, 833, 869, 1112, 1634
HpyF3I	CTNAG	4 cut(s)	663, 810, 1072, 2129
HpySE526I	ACGT	5 cut(s)	144, 367, 445, 1062, 1746
Hsp92I	GRCGYC	1 cut(s)	144
HspAI	GCGC	2 cut(s)	625, 870
Kpn2I	TCCGGA	1 cut(s)	2060
Kzo9I	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
LmnI	GCTCC	2 cut(s)	382, 718
Lsp1109I	GCAGC	4 cut(s)	295, 361, 999, 1621
LweI	GCATC	7 cut(s)	477, 532, 560, 1347, 1869, 1949, 1967
MaeI	CTAG	8 cut(s)	102, 329, 579, 824, 1080, 1809, 1916, 2043
MaeII	ACGT	5 cut(s)	144, 367, 445, 1062, 1746
MaeIII	GTNAC	7 cut(s)	16, 816, 892, 1075, 1460, 1852, 2163
MalI	GATC	8 cut(s)	155, 752, 1317, 1413, 1562, 1668, 1697, 1779
MboI	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
MfeI	CAATTG	1 cut(s)	477
MflI	RGATCY	1 cut(s)	1695
MhlI	GDGCHC	5 cut(s)	273, 888, 1333, 1516, 2137
MlsI	TGGCCA	1 cut(s)	86
MluCI	AATT	8 cut(s)	116, 477, 597, 684, 1528, 1551, 1646, 1866
MluNI	TGGCCA	1 cut(s)	86
MlyI	GAGTC	1 cut(s)	233
MmeI	TCCRAC	2 cut(s)	1273, 1405
Mox20I	TGGCCA	1 cut(s)	86
MroI	TCCGGA	1 cut(s)	2060
MroXI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
MscI	TGGCCA	1 cut(s)	86
MseI	TTAA	5 cut(s)	791, 909, 1128, 1677, 1700
MslI	CAYNNNNRTG	1 cut(s)	355
Msp20I	TGGCCA	1 cut(s)	86
MspI	CCGG	3 cut(s)	189, 1990, 2061
MspR9I	CCNGG	3 cut(s)	426, 703, 1991
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	1 cut(s)	1490
MvaI	CCWGG	2 cut(s)	426, 703
MvnI	CGCG	2 cut(s)	131, 870
MwoI	GCNNNNNNNGC	7 cut(s)	39, 371, 463, 833, 869, 1112, 1634
NciI	CCSGG	1 cut(s)	1991
NcoI	CCATGG	1 cut(s)	617
NdeI	CATATG	1 cut(s)	2068
NdeII	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
NheI	GCTAGC	1 cut(s)	101
NlaIV	GGNNCC	3 cut(s)	359, 885, 1590
NmuCI	GTSAC	2 cut(s)	16, 2163
NspI	RCATGY	3 cut(s)	741, 1908, 2173
NspV	TTCGAA	1 cut(s)	290
PaeI	GCATGC	1 cut(s)	741
PaeR7I	CTCGAG	2 cut(s)	669, 1226
PagI	TCATGA	1 cut(s)	1312
PcsI	WCGNNNNNNNCGW	1 cut(s)	2150
PctI	GAATGC	1 cut(s)	1490
PdmI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
PfeI	GAWTC	2 cut(s)	421, 1805
PflMI	CCANNNNNTGG	1 cut(s)	1522
PfoI	TCCNGGA	1 cut(s)	424
PkrI	GCNGC	5 cut(s)	130, 310, 376, 989, 1636
Ple19I	CGATCG	1 cut(s)	156
PleI	GAGTC	1 cut(s)	232
PpsI	GAGTC	1 cut(s)	232
Ppu21I	YACGTR	1 cut(s)	368
Psp1406I	AACGTT	1 cut(s)	1062
Psp6I	CCWGG	2 cut(s)	424, 701
PspEI	GGTNACC	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	424, 701
PspN4I	GGNNCC	3 cut(s)	359, 885, 1590
PspPI	GGNCC	3 cut(s)	1589, 2024, 2058
PstNI	CAGNNNCTG	1 cut(s)	833
PsuI	RGATCY	1 cut(s)	1695
PvuI	CGATCG	1 cut(s)	156
RsaI	GTAC	1 cut(s)	1377
RsaNI	GTAC	1 cut(s)	1376
RseI	CAYNNNNRTG	1 cut(s)	355
SaqAI	TTAA	5 cut(s)	791, 909, 1128, 1677, 1700
SatI	GCNGC	5 cut(s)	129, 309, 375, 988, 1635
Sau3AI	GATC	8 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695, 1777
Sau96I	GGNCC	3 cut(s)	1589, 2024, 2058
SchI	GAGTC	1 cut(s)	233
ScrFI	CCNGG	3 cut(s)	426, 703, 1991
SduI	GDGCHC	5 cut(s)	273, 888, 1333, 1516, 2137
SfaNI	GCATC	7 cut(s)	477, 532, 560, 1347, 1869, 1949, 1967
SfcI	CTRYAG	2 cut(s)	96, 972
Sfr274I	CTCGAG	2 cut(s)	669, 1226
SfuI	TTCGAA	1 cut(s)	290
SinI	GGWCC	2 cut(s)	1589, 2058
SlaI	CTCGAG	2 cut(s)	669, 1226
SmiI	ATTTAAAT	1 cut(s)	1678
SmiMI	CAYNNNNRTG	1 cut(s)	355
SmlI	CTYRAG	4 cut(s)	558, 669, 1226, 2048
SmoI	CTYRAG	4 cut(s)	558, 669, 1226, 2048
SphI	GCATGC	1 cut(s)	741
Sse9I	AATT	8 cut(s)	116, 477, 597, 684, 1528, 1551, 1646, 1866
SsiI	CCGC	4 cut(s)	129, 985, 1892, 1995
SspMI	CTAG	8 cut(s)	102, 329, 579, 824, 1080, 1809, 1916, 2043
StyD4I	CCNGG	3 cut(s)	424, 701, 1989
StyI	CCWWGG	5 cut(s)	21, 81, 452, 617, 914
SwaI	ATTTAAAT	1 cut(s)	1678
TaaI	ACNGT	4 cut(s)	97, 782, 1423, 1852
TaiI	ACGT	5 cut(s)	147, 370, 448, 1065, 1749
TasI	AATT	8 cut(s)	116, 477, 597, 684, 1528, 1551, 1646, 1866
TauI	GCSGC	1 cut(s)	131
TfiI	GAWTC	2 cut(s)	421, 1805
Tru1I	TTAA	5 cut(s)	791, 909, 1128, 1677, 1700
Tru9I	TTAA	5 cut(s)	791, 909, 1128, 1677, 1700
TscAI	CASTG	5 cut(s)	175, 787, 838, 1253, 2145
TseFI	GTSAC	2 cut(s)	16, 2163
TseI	GCWGC	4 cut(s)	308, 374, 987, 1634
Tsp45I	GTSAC	2 cut(s)	16, 2163
TspDTI	ATGAA	3 cut(s)	573, 758, 1935
TspGWI	ACGGA	4 cut(s)	1393, 1635, 1674, 1746
TspRI	CASTG	5 cut(s)	175, 787, 838, 1253, 2145
Van91I	CCANNNNNTGG	1 cut(s)	1522
VneI	GTGCAC	1 cut(s)	2133
VpaK11BI	GGWCC	2 cut(s)	1589, 2058
XagI	CCTNNNNNAGG	2 cut(s)	429, 662
XapI	RAATTY	2 cut(s)	116, 1866
XbaI	TCTAGA	2 cut(s)	1808, 1915
XceI	RCATGY	3 cut(s)	741, 1908, 2173
XhoI	CTCGAG	2 cut(s)	669, 1226
XmnI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
XspI	CTAG	8 cut(s)	102, 329, 579, 824, 1080, 1809, 1916, 2043
ZraI	GACGTC	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.