Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

14418749 .. 14424675

Gene Structure 5927 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1181900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGCGAAGGTCAAGGTCACCTTGGAGGTCGGAAACGACGGCGTTGCAGTTATCACCATCTTCAATCCCCCTGTCAACGCCTTGGCCATACCCATTATAGCTGGCTTGAAGGAGAAGTTCGAGGAGGCCGGGAGGCGAAACGACGTGAAAGCTATCGTTTTGACTGGCCATGGAGGAAGGTTTTCGGGTGGTTTCGATATCAATGTTTTCGAGAAGGTTCACGAGTCTGGGGATGTTTCGATCATGCCTGATGTATCTGTTGACTTTGTGGTCAACACACTTGAAGATTCGACAAAGCCTGTTGTTGCTGCCGTCCAAGGACTTGCGCTAGGAGGTGGCTTGGAGTTGGCATTGGGGTGCCACGCACGTATTGCTGCTCCAAAAACACAACTTGGCCTTCCGGAGTTGTCACTTGGAGTGATTCCTGGATTTGGAGGCACACAACGTCTTCCTAGGCTTGTAGGGCTTCAGAAGGCAATTGAAATGATGCTGTCATCGAAGCCAATCCTGTCTGAAGAAGGGGAGAAGCTGGGTCTTATAGATGCTATTGTGTCTCCTCAAGAGTTGCTGAAAGTATCTCGCTCTTGGGCTTTGGACATTGCAGATAGGCGCAAACCGTGGTTGCGCTCTCTTCACAGGACAGATAAAATTGGATCCCTGTCTGAGGCACGAGAGATACTGAAAGTTGCCCGACAACAAGCCAAGAAGACTGCTCCCAATATGCCTCAGCACCAAGCATGCCTCAATGTGATTGAGGATGGCGTTATTCATGGAGGATACAGTGGTGTTTTAACGGAGGCTAAAGTTTTTAAGGAGTTAGTTCTAGCGGACACTTCAAAAGGTCTTGTCCATGTATTTTTTGCTCAACGCGCAACATCTAAGGGTGTGCCCAACGTTACTGATGTTGGCCTTAAACCAAGGCAAATAAAGAAAGTTGCTGTTATTGGGGGAGGTCTGATGGGTTCTGGCATTGCTACGGCCCTTCTTGTGAGCAACATATCTGTGGTTCTAAAGGAAGTCAACTCTGAATATCTTCAGAAGGGCATAAAAACGATAGAAGGAAATGTTAAAGGCTTAGTAACTAGAAGGAGGCTGACACAGGACAAGGCACAAAAAGCTCTCTTATTGCTTAAAGGTAGTTTGGACTACTCCGACTTCAAAGATGTGGACATGGTCATAGAGGCTGTCATTGAGAGTGTTCCTCTCAAACAGAAAATTTTTAGTGAACTCGAGAAAGTTTGCCCTTCTCACTGTGTCTTGGCTACAAATACATCCACTATTGACCTCAATGTTGTTGGGGAAAAGACAAACTCCCATGATCGCATTGTAGGGGCACATTTTTTCAGTCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCGGCACAAGTAATTCTTGATCTTTTGACTGTTGGAAAAATAATAAAGAAAGTTCCAGTTGTGGTTGGTAACTGCACAGGCTTTGCAGTCAATCGAGCATTCTTTCCCTACACACAAGGTGCCCATATATTGGTCAATTCAGGTGTGGATGTATTCAGAATTGATAGGGTCATCAGCGATTTTGGCCTACCTATGGGTCCATTCCAGCTTCAGGACTTGGCAGGCTATGGAGTAGCCCTTGCAGTTGGAAAGGAATTTTCTGGTGCTTTCCCTGATCGCACATTTAGATCGCCATTGGTTGAGATCTTGATAAAAAATGGACGAAATGGTAAAAACAATGGAAAAGGATATTACATTTATGAGAAGGGTAGCAAGCCAAAACCTGATCCTTCTGTCCTACCACTCATTGAGGAATCTCGACGAGTTACCAACATCATGCCTGGTGGAAAGCCTTTAACTGTTACAGACCAAGATATTCTGGAGGTGATACTGTTTCCTGTAGTAAATGAGGCATGCCGTATTCTAGATGAAGGAATAGTTATTCGAGCAGCAGACCTTGACATCGCATCTGTTCTTGGGATGAGTTTCCCATCTTACCGGGGGGGTATTGTCTTTTGGGCAGATCTGGTCGGGCCTAAGCATATATACGCTAGTCTCAAGAAATGGTCCGGAGTATATGGCAACTTTTTCAAACCATCCAGGTTTTTGGAAGAAAGAGCAACAAGAGGGTTGCCGCTGAGTGCACCCGCGTCGTCATCCGCATCTTCAAGGTCACGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

726

Amino Acids

78.21

Weight (kDa)

9.28

Isoelectric Point (pI)

37.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	12 - 211	2.8e-40	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 195	2e-20	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	311 - 489	4.7e-57	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	492 - 585	9.2e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	269
AccB1I	GGYRCC	2 cut(s)	357, 1514
AccB7I	CCANNNNNTGG	1 cut(s)	1525
AccII	CGCG	2 cut(s)	870, 2144
AccIII	TCCGGA	2 cut(s)	400, 2063
AciI	CCGC	4 cut(s)	827, 2129, 2142, 2154
AclI	AACGTT	1 cut(s)	894
AclWI	GGATC	3 cut(s)	648, 661, 1775
AcoI	YGGCCR	2 cut(s)	84, 166
AcsI	RAATTY	2 cut(s)	1215, 1649
AcuI	CTGAAG	4 cut(s)	452, 534, 1019, 1589
AdeI	CACNNNGTG	1 cut(s)	1514
AfaI	GTAC	1 cut(s)	1380
AfiI	CCNNNNNNNGG	5 cut(s)	431, 664, 1525, 1588, 1606
AgsI	TTSAA	9 cut(s)	64, 109, 284, 482, 837, 1159, 1373, 2086, 2163
AjiI	CACGTC	1 cut(s)	145
AjnI	CCWGG	3 cut(s)	424, 1834, 2093
AluBI	AGCT	5 cut(s)	101, 152, 529, 1118, 1603
AluI	AGCT	5 cut(s)	101, 152, 529, 1118, 1603
Alw21I	GWGCWC	1 cut(s)	2140
Alw26I	GTCTC	2 cut(s)	558, 2054
Alw44I	GTGCAC	1 cut(s)	2136
AlwI	GGATC	3 cut(s)	648, 661, 1775
Ama87I	CYCGRG	1 cut(s)	1229
Aor13HI	TCCGGA	2 cut(s)	400, 2063
AoxI	GGCC	8 cut(s)	84, 126, 166, 394, 907, 978, 1579, 2027
ApaLI	GTGCAC	1 cut(s)	2136
ApeKI	GCWGC	3 cut(s)	308, 374, 1943
ApoI	RAATTY	2 cut(s)	1215, 1649
Asp700I	GAANNNNTTC	2 cut(s)	181, 1032
AspA2I	CCTAGG	1 cut(s)	452
AspLEI	GCGC	4 cut(s)	328, 612, 627, 872
AspS9I	GGNCC	4 cut(s)	979, 1592, 2027, 2061
AsuC2I	CCSGG	2 cut(s)	130, 1994
AsuHPI	GGTGA	3 cut(s)	10, 46, 1891
AvaI	CYCGRG	1 cut(s)	1229
AvaII	GGWCC	2 cut(s)	1592, 2061
AvrII	CCTAGG	1 cut(s)	452
BaeGI	GKGCMC	4 cut(s)	891, 1336, 1519, 2140
BaeI	ACNNNNGTAYC	2 cut(s)	769, 802
BalI	TGGCCA	2 cut(s)	86, 168
BamHI	GGATCC	1 cut(s)	653
BanI	GGYRCC	2 cut(s)	357, 1514
BauI	CACGAG	2 cut(s)	221, 669
BbsI	GAAGAC	3 cut(s)	440, 713, 1397
Bbv12I	GWGCWC	1 cut(s)	2140
BbvCI	CCTCAGC	1 cut(s)	726
BbvI	GCAGC	3 cut(s)	295, 361, 1955
BccI	CCATC	5 cut(s)	65, 752, 952, 1993, 2098
BceAI	ACGGC	4 cut(s)	55, 296, 993, 1896
BcgI	CGANNNNNNTGC	4 cut(s)	26, 60, 2127, 2161
BciT130I	CCWGG	3 cut(s)	426, 1836, 2095
BciVI	GTATCC	1 cut(s)	770
BcnI	CCSGG	2 cut(s)	130, 1994
BcoDI	GTCTC	2 cut(s)	558, 2054
BfaI	CTAG	6 cut(s)	329, 453, 824, 1083, 1919, 2046
BfmI	CTRYAG	1 cut(s)	1893
BfuI	GTATCC	1 cut(s)	770
BglII	AGATCT	2 cut(s)	1698, 2017
BisI	GCNGC	4 cut(s)	309, 375, 1944, 2129
BlnI	CCTAGG	1 cut(s)	452
BlsI	GCNGC	4 cut(s)	310, 376, 1945, 2130
Bme1390I	CCNGG	5 cut(s)	130, 426, 1836, 1994, 2095
Bme18I	GGWCC	2 cut(s)	1592, 2061
BmeT110I	CYCGRG	1 cut(s)	1229
BmgBI	CACGTC	1 cut(s)	145
BmgT120I	GGNCC	4 cut(s)	979, 1592, 2027, 2061
BmiI	GGNNCC	4 cut(s)	359, 655, 1516, 1593
BmrFI	CCNGG	5 cut(s)	130, 426, 1836, 1994, 2095
BmsI	GCATC	5 cut(s)	477, 532, 1350, 1970, 2165
BpiI	GAAGAC	3 cut(s)	440, 713, 1397
BplI	GAGNNNNNCTC	2 cut(s)	1186, 1218
BpmI	CTGGAG	1 cut(s)	1895
Bpu10I	CCTNAGC	2 cut(s)	726, 2031
BpuEI	CTTGAG	2 cut(s)	543, 2036
BpuMI	CCSGG	2 cut(s)	130, 1994
BsaAI	YACGTR	1 cut(s)	368
BsaJI	CCNNGG	8 cut(s)	21, 81, 169, 316, 452, 617, 917, 1993
BsaWI	WCCGGW	2 cut(s)	400, 2063
Bsc4I	CCNNNNNNNGG	5 cut(s)	431, 664, 1525, 1588, 1606
Bse1I	ACTGG	2 cut(s)	169, 1451
Bse3DI	GCAATG	2 cut(s)	597, 969
BseAI	TCCGGA	2 cut(s)	400, 2063
BseBI	CCWGG	3 cut(s)	426, 1836, 2095
BseDI	CCNNGG	8 cut(s)	21, 81, 169, 316, 452, 617, 917, 1993
BseGI	GGATG	7 cut(s)	238, 763, 1271, 1549, 1980, 2090, 2150
BseLI	CCNNNNNNNGG	5 cut(s)	431, 664, 1525, 1588, 1606
BseMI	GCAATG	2 cut(s)	597, 969
BseMII	CTCAG	3 cut(s)	654, 740, 2123
BseNI	ACTGG	2 cut(s)	169, 1451
BseRI	GAGGAG	2 cut(s)	137, 546
BseSI	GKGCMC	4 cut(s)	891, 1336, 1519, 2140
BseXI	GCAGC	3 cut(s)	295, 361, 1955
BseYI	CCCAGC	1 cut(s)	529
BsgI	GTGCAG	1 cut(s)	1453
Bsh1236I	CGCG	2 cut(s)	870, 2144
BshFI	GGCC	8 cut(s)	86, 128, 168, 396, 909, 980, 1581, 2029
BshNI	GGYRCC	2 cut(s)	357, 1514
BsiHKAI	GWGCWC	1 cut(s)	2140
BsiHKCI	CYCGRG	1 cut(s)	1229
BsiSI	CCGG	4 cut(s)	129, 401, 1993, 2064
BslI	CCNNNNNNNGG	5 cut(s)	431, 664, 1525, 1588, 1606
BsmAI	GTCTC	2 cut(s)	558, 2054
BsmI	GAATGC	1 cut(s)	1493
BsnI	GGCC	8 cut(s)	86, 128, 168, 396, 909, 980, 1581, 2029
BsoBI	CYCGRG	1 cut(s)	1229
Bsp1286I	GDGCHC	4 cut(s)	891, 1336, 1519, 2140
Bsp13I	TCCGGA	2 cut(s)	400, 2063
Bsp143I	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
Bsp19I	CCATGG	1 cut(s)	169
BspACI	CCGC	4 cut(s)	827, 2129, 2142, 2154
BspANI	GGCC	8 cut(s)	86, 128, 168, 396, 909, 980, 1581, 2029
BspCNI	CTCAG	3 cut(s)	655, 739, 2124
BspEI	TCCGGA	2 cut(s)	400, 2063
BspFNI	CGCG	2 cut(s)	870, 2144
BspLI	GGNNCC	4 cut(s)	359, 655, 1516, 1593
BspPI	GGATC	3 cut(s)	648, 661, 1775
BspT107I	GGYRCC	2 cut(s)	357, 1514
BsrDI	GCAATG	2 cut(s)	597, 969
BsrI	ACTGG	2 cut(s)	169, 1451
BssECI	CCNNGG	8 cut(s)	21, 81, 169, 316, 452, 617, 917, 1993
BssMI	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
BssSI	CACGAG	2 cut(s)	221, 669
BssT1I	CCWWGG	6 cut(s)	21, 81, 169, 316, 452, 917
Bst2BI	CACGAG	2 cut(s)	221, 669
Bst2UI	CCWGG	3 cut(s)	426, 1836, 2095
Bst4CI	ACNGT	6 cut(s)	618, 782, 1253, 1426, 1855, 1887
Bst6I	CTCTTC	2 cut(s)	636, 1371
BstAPI	GCANNNNNTGC	1 cut(s)	371
BstBAI	YACGTR	1 cut(s)	368
BstC8I	GCNNGC	5 cut(s)	103, 739, 1618, 1769, 1909
BstDEI	CTNAG	6 cut(s)	663, 726, 879, 1075, 2031, 2132
BstDSI	CCRYGG	2 cut(s)	169, 617
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	1 cut(s)	662
BstF5I	GGATG	7 cut(s)	238, 763, 1271, 1549, 1980, 2090, 2150
BstFNI	CGCG	2 cut(s)	870, 2144
BstHHI	GCGC	4 cut(s)	328, 612, 627, 872
BstKTI	GATC	9 cut(s)	243, 656, 1321, 1417, 1672, 1685, 1701, 1783, 2020
BstMAI	GTCTC	2 cut(s)	558, 2054
BstMBI	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
BstMWI	GCNNNNNNNGC	5 cut(s)	371, 463, 869, 1115, 1578
BstNI	CCWGG	3 cut(s)	426, 1836, 2095
BstNSI	RCATGY	3 cut(s)	741, 1911, 2176
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	5 cut(s)	128, 424, 1834, 1992, 2093
BstSFI	CTRYAG	1 cut(s)	1893
BstSLI	GKGCMC	4 cut(s)	891, 1336, 1519, 2140
BstUI	CGCG	2 cut(s)	870, 2144
BstV1I	GCAGC	3 cut(s)	295, 361, 1955
BstV2I	GAAGAC	3 cut(s)	440, 713, 1397
BstX2I	RGATCY	3 cut(s)	653, 1698, 2017
BstXI	CCANNNNNNTGG	1 cut(s)	2101
BstYI	RGATCY	3 cut(s)	653, 1698, 2017
BsuI	GTATCC	1 cut(s)	770
BsuRI	GGCC	8 cut(s)	86, 128, 168, 396, 909, 980, 1581, 2029
BtgI	CCRYGG	2 cut(s)	169, 617
BtgZI	GCGATG	1 cut(s)	1942
BtrI	CACGTC	1 cut(s)	145
BtsCI	GGATG	7 cut(s)	238, 763, 1271, 1549, 1980, 2090, 2150
BtsIMutI	CAGTG	2 cut(s)	787, 1249
Cac8I	GCNNGC	5 cut(s)	103, 739, 1618, 1769, 1909
CfoI	GCGC	4 cut(s)	328, 612, 627, 872
Cfr13I	GGNCC	4 cut(s)	979, 1592, 2027, 2061
CseI	GACGC	1 cut(s)	2133
Csp6I	GTAC	1 cut(s)	1379
CviQI	GTAC	1 cut(s)	1379
DdeI	CTNAG	6 cut(s)	663, 726, 879, 1075, 2031, 2132
DpnI	GATC	9 cut(s)	242, 655, 1320, 1416, 1671, 1684, 1700, 1782, 2019
DpnII	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
DraIII	CACNNNGTG	1 cut(s)	1514
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	2 cut(s)	84, 166
Eam1104I	CTCTTC	2 cut(s)	636, 1371
EarI	CTCTTC	2 cut(s)	636, 1371
Eco130I	CCWWGG	6 cut(s)	21, 81, 169, 316, 452, 917
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	2 cut(s)	1592, 2061
Eco57I	CTGAAG	4 cut(s)	452, 534, 1019, 1589
Eco88I	CYCGRG	1 cut(s)	1229
Eco91I	GGTNACC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	1 cut(s)	662
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	3 cut(s)	424, 1834, 2093
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	6 cut(s)	21, 81, 169, 316, 452, 917
ErhI	CCWWGG	6 cut(s)	21, 81, 169, 316, 452, 917
FalI	AAGNNNNNCTT	3 cut(s)	37, 1395, 1427
FauI	CCCGC	1 cut(s)	2149
Fnu4HI	GCNGC	4 cut(s)	309, 375, 1944, 2129
FokI	GGATG	7 cut(s)	245, 770, 1258, 1556, 1987, 2077, 2137
Fsp4HI	GCNGC	4 cut(s)	309, 375, 1944, 2129
FspBI	CTAG	6 cut(s)	329, 453, 824, 1083, 1919, 2046
GlaI	GCGC	4 cut(s)	327, 611, 626, 871
GluI	GCNGC	4 cut(s)	309, 375, 1944, 2129
GsaI	CCCAGC	1 cut(s)	533
GsuI	CTGGAG	1 cut(s)	1895
HaeIII	GGCC	8 cut(s)	86, 128, 168, 396, 909, 980, 1581, 2029
HapII	CCGG	4 cut(s)	129, 401, 1993, 2064
HgaI	GACGC	1 cut(s)	2133
HhaI	GCGC	4 cut(s)	328, 612, 627, 872
Hin6I	GCGC	4 cut(s)	326, 610, 625, 870
HinP1I	GCGC	4 cut(s)	326, 610, 625, 870
HincII	GTYRAC	4 cut(s)	76, 262, 274, 1021
HindII	GTYRAC	4 cut(s)	76, 262, 274, 1021
HinfI	GANTC	4 cut(s)	224, 287, 421, 1808
HpaII	CCGG	4 cut(s)	129, 401, 1993, 2064
HphI	GGTGA	3 cut(s)	10, 46, 1891
Hpy166II	GTNNAC	8 cut(s)	76, 220, 262, 274, 1021, 1168, 1226, 2138
Hpy188I	TCNGA	9 cut(s)	32, 471, 514, 664, 957, 1027, 1038, 1153, 1553
Hpy8I	GTNNAC	8 cut(s)	76, 220, 262, 274, 1021, 1168, 1226, 2138
Hpy99I	CGWCG	4 cut(s)	41, 146, 1818, 2149
HpyCH4III	ACNGT	6 cut(s)	618, 782, 1253, 1426, 1855, 1887
HpyCH4IV	ACGT	4 cut(s)	144, 367, 445, 894
HpyCH4V	TGCA	6 cut(s)	47, 602, 1470, 1481, 1637, 2138
HpyF10VI	GCNNNNNNNGC	5 cut(s)	371, 463, 869, 1115, 1578
HpyF3I	CTNAG	6 cut(s)	663, 726, 879, 1075, 2031, 2132
HpySE526I	ACGT	4 cut(s)	144, 367, 445, 894
HspAI	GCGC	4 cut(s)	326, 610, 625, 870
Kpn2I	TCCGGA	2 cut(s)	400, 2063
Kzo9I	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
LmnI	GCTCC	2 cut(s)	382, 718
Lsp1109I	GCAGC	3 cut(s)	295, 361, 1955
LweI	GCATC	5 cut(s)	477, 532, 1350, 1970, 2165
MaeI	CTAG	6 cut(s)	329, 453, 824, 1083, 1919, 2046
MaeII	ACGT	4 cut(s)	144, 367, 445, 894
MaeIII	GTNAC	8 cut(s)	16, 408, 895, 1078, 1463, 1819, 1855, 2166
MalI	GATC	9 cut(s)	242, 655, 1320, 1416, 1671, 1684, 1700, 1782, 2019
MboI	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
MfeI	CAATTG	1 cut(s)	477
MflI	RGATCY	3 cut(s)	653, 1698, 2017
MhlI	GDGCHC	4 cut(s)	891, 1336, 1519, 2140
MlsI	TGGCCA	2 cut(s)	86, 168
MluCI	AATT	7 cut(s)	477, 648, 1215, 1407, 1531, 1554, 1649
MluNI	TGGCCA	2 cut(s)	86, 168
MlyI	GAGTC	1 cut(s)	233
MmeI	TCCRAC	4 cut(s)	10, 1176, 1408, 1621
Mox20I	TGGCCA	2 cut(s)	86, 168
MroI	TCCGGA	2 cut(s)	400, 2063
MroXI	GAANNNNTTC	2 cut(s)	181, 1032
MscI	TGGCCA	2 cut(s)	86, 168
MseI	TTAA	6 cut(s)	791, 810, 912, 1068, 1131, 1850
MslI	CAYNNNNRTG	2 cut(s)	355, 1001
Msp20I	TGGCCA	2 cut(s)	86, 168
MspA1I	CMGCKG	1 cut(s)	2131
MspI	CCGG	4 cut(s)	129, 401, 1993, 2064
MspR9I	CCNGG	5 cut(s)	130, 426, 1836, 1994, 2095
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	1 cut(s)	1493
MvaI	CCWGG	3 cut(s)	426, 1836, 2095
MvnI	CGCG	2 cut(s)	870, 2144
MwoI	GCNNNNNNNGC	5 cut(s)	371, 463, 869, 1115, 1578
NciI	CCSGG	2 cut(s)	130, 1994
NcoI	CCATGG	1 cut(s)	169
NdeII	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
NlaIV	GGNNCC	4 cut(s)	359, 655, 1516, 1593
NmuCI	GTSAC	3 cut(s)	16, 408, 2166
NspI	RCATGY	3 cut(s)	741, 1911, 2176
PaeI	GCATGC	2 cut(s)	741, 1911
PaeR7I	CTCGAG	1 cut(s)	1229
PctI	GAATGC	1 cut(s)	1493
PdmI	GAANNNNTTC	2 cut(s)	181, 1032
PfeI	GAWTC	3 cut(s)	287, 421, 1808
PflFI	GACNNNGTC	1 cut(s)	1172
PflMI	CCANNNNNTGG	1 cut(s)	1525
PfoI	TCCNGGA	1 cut(s)	424
PkrI	GCNGC	4 cut(s)	310, 376, 1945, 2130
PleI	GAGTC	1 cut(s)	232
PpsI	GAGTC	1 cut(s)	232
Ppu21I	YACGTR	1 cut(s)	368
Psp1406I	AACGTT	1 cut(s)	894
Psp6I	CCWGG	3 cut(s)	424, 1834, 2093
PspEI	GGTNACC	1 cut(s)	16
PspFI	CCCAGC	1 cut(s)	529
PspGI	CCWGG	3 cut(s)	424, 1834, 2093
PspN4I	GGNNCC	4 cut(s)	359, 655, 1516, 1593
PspPI	GGNCC	4 cut(s)	979, 1592, 2027, 2061
PsuI	RGATCY	3 cut(s)	653, 1698, 2017
PsyI	GACNNNGTC	1 cut(s)	1172
RsaI	GTAC	1 cut(s)	1380
RsaNI	GTAC	1 cut(s)	1379
RseI	CAYNNNNRTG	2 cut(s)	355, 1001
SaqAI	TTAA	6 cut(s)	791, 810, 912, 1068, 1131, 1850
SatI	GCNGC	4 cut(s)	309, 375, 1944, 2129
Sau3AI	GATC	9 cut(s)	240, 653, 1318, 1414, 1669, 1682, 1698, 1780, 2017
Sau96I	GGNCC	4 cut(s)	979, 1592, 2027, 2061
SchI	GAGTC	1 cut(s)	233
ScrFI	CCNGG	5 cut(s)	130, 426, 1836, 1994, 2095
SduI	GDGCHC	4 cut(s)	891, 1336, 1519, 2140
SfaNI	GCATC	5 cut(s)	477, 532, 1350, 1970, 2165
SfcI	CTRYAG	1 cut(s)	1893
Sfr274I	CTCGAG	1 cut(s)	1229
SinI	GGWCC	2 cut(s)	1592, 2061
SlaI	CTCGAG	1 cut(s)	1229
SmiMI	CAYNNNNRTG	2 cut(s)	355, 1001
SmlI	CTYRAG	3 cut(s)	558, 1229, 2051
SmoI	CTYRAG	3 cut(s)	558, 1229, 2051
SphI	GCATGC	2 cut(s)	741, 1911
Sse9I	AATT	7 cut(s)	477, 648, 1215, 1407, 1531, 1554, 1649
SsiI	CCGC	4 cut(s)	827, 2129, 2142, 2154
SspMI	CTAG	6 cut(s)	329, 453, 824, 1083, 1919, 2046
StyD4I	CCNGG	5 cut(s)	128, 424, 1834, 1992, 2093
StyI	CCWWGG	6 cut(s)	21, 81, 169, 316, 452, 917
TaaI	ACNGT	6 cut(s)	618, 782, 1253, 1426, 1855, 1887
TaiI	ACGT	4 cut(s)	147, 370, 448, 897
TasI	AATT	7 cut(s)	477, 648, 1215, 1407, 1531, 1554, 1649
TauI	GCSGC	1 cut(s)	2131
TfiI	GAWTC	3 cut(s)	287, 421, 1808
Tru1I	TTAA	6 cut(s)	791, 810, 912, 1068, 1131, 1850
Tru9I	TTAA	6 cut(s)	791, 810, 912, 1068, 1131, 1850
TscAI	CASTG	2 cut(s)	787, 1256
TseFI	GTSAC	3 cut(s)	16, 408, 2166
TseI	GCWGC	3 cut(s)	308, 374, 1943
Tsp45I	GTSAC	3 cut(s)	16, 408, 2166
TspDTI	ATGAA	2 cut(s)	758, 1938
TspGWI	ACGGA	2 cut(s)	809, 1396
TspRI	CASTG	2 cut(s)	787, 1256
Tth111I	GACNNNGTC	1 cut(s)	1172
Van91I	CCANNNNNTGG	1 cut(s)	1525
VneI	GTGCAC	1 cut(s)	2136
VpaK11BI	GGWCC	2 cut(s)	1592, 2061
XagI	CCTNNNNNAGG	1 cut(s)	662
XapI	RAATTY	2 cut(s)	1215, 1649
XbaI	TCTAGA	1 cut(s)	1918
XceI	RCATGY	3 cut(s)	741, 1911, 2176
XhoI	CTCGAG	1 cut(s)	1229
XmaJI	CCTAGG	1 cut(s)	452
XmnI	GAANNNNTTC	2 cut(s)	181, 1032
XspI	CTAG	6 cut(s)	329, 453, 824, 1083, 1919, 2046

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.