MD02G1043500.v1.1
NAC Family

proteinaceous RNase P 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002433
Physical Location & Seq
Forward (+)
3556977 .. 3560796
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1043500.v1.1.491

Sequence Viewer

Length: 2637 bp
ATGGAGCTCTTCCAACGGCCACACATGGCCTCCTTCACCTCCAACGCACTGCAGCAAAAGCACCACCACCACCACCTCCTCTCCGCCACCCTCTGTAAGTGCCCGTCAGCTTTCCACGTCTTTAAATTCCGTTTTCCTTCTCATATTTTCACTTTCTCGCCACCCAAACAGACCCTTAATCTTTATCCACTTCCACTAGCTAGACAAAACATTAGCCACATAGTTGCAAAGTTATCCTCGACAACGTACCACGAACCGTACACAAAATCGCAGAACTCAGGAACTGGAAGTGGTTCGGAGTTTTCTTCCTTAAAGTCCACGGAACAACGGGTTTCGAGAAAACCCGTAAAGAACCATGTCGGTTCAGGAGTGGAGAAGAAGGGTGAAGATACTAGGAAAAATCTTGTCTTTAGAAAGAGGAGGGAGGGGAATGGTCCTAATGGGCCAAATGGGCCAAATGGGCATTCTTCTAGGAGGTTTAGAGATGAGAATGCGGTAAAATCTTCGAAAAGCCGAGGCAGTTTAGGGGTAAAGGAGGGGAGGAACAAGAGGGGTGGTGGTAATCAGGTGGGAGGAGAAGAGAGAAGGGGGAGAGGGTTGAAGAAGTATGACGCTGATTTGCCGGAAGTTAAGATGCGAGTAGGTTTGGATATGTGCTCGAAGAGAGGGGATGTGATGGGTGCAATTAGGTTCTATGATTTGGCTCAAACGGAAGAGATTAAGTTGGAGCAGTACCATTATACTGTGCTGTTGTATCTCTGCTCTTCTGCAGCTGTTGGTGTTGTTCGTCCGGCGAAGAGTGGGAGTGGGAGTCGGACTTTGGATACCTTGGATTCGCACAGTGAAGAAACTAGGGTGGGATCCATGGATACATCAAATTTGGACAATGAGGACCTGGATGATACCTGTGGAAGTAACAGTTTTGATGATGATTCGGATGGTACTTCCGATGAAAATGAGAATTTAGCATGGTTTTCTAATGGGTTTGTGAAGCGAAATTCCCGCCTTTTAGATGGACTGAACTACCCCACAAAGGGTGGAGATGATTCTTCTAATGTAAAAGAAGGGAGCAGTAAGCAAGAAGATAATGGAATTCGGGTTAGTGAAGATGTGAAGAAGTATGCGCTCAAAAGGGGATTTGAGATCTATGAGAAGATGTGTTCGGATAATGTTCCGGTGAATGAGGCAGCATTGACATCTGTGGCTAGAATGGCAATGTCAATGGGTGATGGAGACATGGCATTTGATATGGTGAGGCAAATGAAGTCAATGGGAATAAATCCTAGACTCCGTTCCTACGGTCCTGCATTATCTGCTTTTTGCCATAGCGGAGATATTGATAAAGCATTTGCTGTTGAGAAACACATGTTGGAGCATGGTGTTTATCCGGAAGAGCCTGAACTTGAAGCCCTCTTAAGAGTGAGTGTGGGAGTTGGTAAAGGCGACAAGGTGTATTATATGCTGCACAAACTAAGAACAAGCGTAAGGAGGGTGTCGCCGTCTACTGCAGATTTAATCGTAAAATGGTTTCTTAGTAAGGAAGCTTCAAGAGTGGGGAGAACAAAATGGGATCGAAGGTCTATAAGGGATGCAATCGAGAATGGAGGTGGGGGCTGGCATGGGCAGGGCTGGTTGGGAAAAGGGAAGTGGTCTGTGTTACGTACTACAATCGGAGATGATGGCTTGTGCAAATGCTGTGGGGAAAAACTGGCCACAATTGACCTTGATCCCGTAGAAACAGAAAACTTTGCCGAGTCAGTTGCATCAATAGCTATAAAAAGAGAGAAAAACTCAAGCTTTCAGAAATTTCAAAAATGGCTGGACTATTATGGACCTTTTGAAGCAGTGGTGGATGGAGCTAATGTAGGCCTTTTCAGTCAGAAGAAATTTGTCCCGTCCAAGGTCAATGCTGTTGTTAATGGAATACGGCAGAAACTTCCTTCAAAGAAATGGCCACTTATTGTTCTTCATAATAGGCGCATCACCGGAGGCAGAATGGATGAGAGAGTAAATAGGGCACTGATTGAGAAGTGGCAAAATGCCGATGCTCTATATGCAACACCTACTGGTTCAAATGATGATTGGTACTGGTTGTATGCAGCTATAAAGTTCAAGTGCTTACTTGTGACAAATGATGAGATGAGAGACCATATATTTCAACTTCTCGGAAATGATTTCTTTCCCAGATGGAAAGAAAGGCATCAAGTGCATTTCACTTTTTCTGATGCTGGTCCAGTCTTTCACATGCCTCCTCCCTGTTCTGTTGTAATACAGGAATCAGAGGAAGGGCACTGGCACATTCCAGTTGTATCAGAACATGAGCGCGAATCAGAAAGAACATGGCTATGCATTATGCGAGCTAAATCACGCACAGCAACAAAGGATTCTGCAGCCATCCCTGAAGATGCACAACCCCCTCGTCATAACGGATATGCAAGGTCAGCCACCCGAACTGGTGTTGATTCACAGCCACTGAATAGTGGCAACAAAAAATATTCTAAACACAAACCTAAGGAATTTATCAAGAATGTCAAAGATATTCTGTTGGGGTCCGTGACCTCGGATCATCACTCGATTGTGCCAGACATTGCAACTGCAGAGAAGATTGGTGGATGCACCATTGATTTCCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PPR_long PRORP

Protein Analysis

879

Amino Acids

98.2

Weight (kDa)

9.07

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 205 - 261 2.1e-13 Pentacotripeptide-repeat region of PRORP
PPR_long PF17177 371 - 516 6.7e-53 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 397 - 511 2.4e-08 Fungal tetratrico peptide repeats
PRORP PF16953 557 - 783 3.6e-81 Protein-only RNase P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1502
AccII CGCG 1 cut(s) 2325
AccIII TCCGGA 1 cut(s) 1387
AciI CCGC 4 cut(s) 84, 494, 1003, 1329
AclWI GGATC 5 cut(s) 855, 868, 1578, 1721, 2571
AcoI YGGCCR 3 cut(s) 17, 1710, 1952
AcsI RAATTY 8 cut(s) 125, 877, 961, 997, 1092, 1805, 1886, 2516
AcuI CTGAAG 1 cut(s) 2421
AfaI GTAC 6 cut(s) 248, 260, 734, 943, 1663, 2087
AfiI CCNNNNNNNGG 3 cut(s) 1033, 1034, 1900
AflII CTTAAG 1 cut(s) 1414
AflIII ACRYGT 1 cut(s) 1365
AgsI TTSAA 9 cut(s) 601, 1406, 1548, 1811, 1841, 1944, 2073, 2113, 2159
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 1 cut(s) 894
AjuI GAANNNNNNNTTGG 4 cut(s) 448, 480, 1352, 1384
Alw21I GWGCWC 2 cut(s) 9, 659
Alw26I GTCTC 2 cut(s) 1227, 2139
AlwI GGATC 5 cut(s) 855, 868, 1578, 1721, 2571
AlwNI CAGNNNCTG 2 cut(s) 284, 2473
Aor13HI TCCGGA 1 cut(s) 1387
AoxI GGCC 7 cut(s) 17, 27, 443, 452, 1710, 1867, 1952
ApeKI GCWGC 6 cut(s) 52, 770, 1187, 1462, 2099, 2390
ApoI RAATTY 8 cut(s) 125, 877, 961, 997, 1092, 1805, 1886, 2516
ArsI GACNNNNNNTTYG 2 cut(s) 1226, 1258
Asp700I GAANNNNTTC 1 cut(s) 292
AspLEI GCGC 3 cut(s) 1126, 1980, 2325
AspS9I GGNCC 8 cut(s) 434, 443, 452, 892, 1301, 1832, 2231, 2550
AsuHPI GGTGA 6 cut(s) 28, 395, 1189, 1238, 1264, 1975
AsuII TTCGAA 1 cut(s) 506
AvaII GGWCC 6 cut(s) 434, 892, 1301, 1832, 2231, 2550
AxyI CCTNAGG 1 cut(s) 2511
BaeGI GKGCMC 3 cut(s) 104, 2020, 2292
BalI TGGCCA 2 cut(s) 1712, 1954
BamHI GGATCC 1 cut(s) 860
BanII GRGCYC 1 cut(s) 9
BarI GAAGNNNNNNTAC 2 cut(s) 1528, 1560
Bbv12I GWGCWC 2 cut(s) 9, 659
BbvI GCAGC 6 cut(s) 64, 782, 1199, 1449, 2111, 2402
BccI CCATC 8 cut(s) 670, 932, 1007, 1223, 1673, 1847, 2181, 2402
BceAI ACGGC 3 cut(s) 32, 1483, 1943
BcgI CGANNNNNNTGC 2 cut(s) 1742, 1776
BciT130I CCWGG 1 cut(s) 896
BciVI GTATCC 2 cut(s) 817, 862
BcoDI GTCTC 2 cut(s) 1227, 2139
BfaI CTAG 7 cut(s) 197, 201, 393, 471, 852, 1206, 1284
BfmI CTRYAG 5 cut(s) 50, 768, 1506, 2388, 2595
BfrI CTTAAG 1 cut(s) 1414
BfuI GTATCC 2 cut(s) 817, 862
BglI GCCNNNNNGGC 2 cut(s) 451, 460
BglII AGATCT 1 cut(s) 1143
BisI GCNGC 6 cut(s) 53, 771, 1188, 1463, 2100, 2391
BlsI GCNGC 6 cut(s) 54, 772, 1189, 1464, 2101, 2392
Bme1390I CCNGG 1 cut(s) 896
Bme18I GGWCC 6 cut(s) 434, 892, 1301, 1832, 2231, 2550
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 8 cut(s) 434, 443, 452, 892, 1301, 1832, 2231, 2550
BmiI GGNNCC 2 cut(s) 862, 2551
BmrFI CCNGG 1 cut(s) 896
BmsI GCATC 9 cut(s) 624, 1579, 1772, 1989, 2035, 2209, 2215, 2395, 2603
BplI GAGNNNNNCTC 2 cut(s) 1775, 1807
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 1777
BsaAI YACGTR 1 cut(s) 1661
BsaI GGTCTC 1 cut(s) 2139
BsaJI CCNNGG 6 cut(s) 318, 514, 828, 864, 1899, 2559
BsaWI WCCGGW 3 cut(s) 1174, 1387, 1985
BsaXI ACNNNNNCTCC 8 cut(s) 14, 44, 65, 95, 796, 826, 1848, 1878
Bsc4I CCNNNNNNNGG 3 cut(s) 1033, 1034, 1900
Bse1I ACTGG 8 cut(s) 289, 1713, 2071, 2093, 2234, 2297, 2303, 2458
Bse21I CCTNAGG 1 cut(s) 2511
Bse3DI GCAATG 2 cut(s) 1221, 2586
BseAI TCCGGA 1 cut(s) 1387
BseBI CCWGG 1 cut(s) 896
BseDI CCNNGG 6 cut(s) 318, 514, 828, 864, 1899, 2559
BseGI GGATG 8 cut(s) 676, 904, 943, 1594, 1858, 2005, 2394, 2618
BseLI CCNNNNNNNGG 3 cut(s) 1033, 1034, 1900
BseMI GCAATG 2 cut(s) 1221, 2586
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 8 cut(s) 289, 1713, 2071, 2093, 2234, 2297, 2303, 2458
BseRI GAGGAG 4 cut(s) 68, 433, 588, 2241
BseSI GKGCMC 3 cut(s) 104, 2020, 2292
BseXI GCAGC 6 cut(s) 64, 782, 1199, 1449, 2111, 2402
BsgI GTGCAG 1 cut(s) 1448
Bsh1236I CGCG 1 cut(s) 2325
BshFI GGCC 7 cut(s) 19, 29, 445, 454, 1712, 1869, 1954
BsiHKAI GWGCWC 2 cut(s) 9, 659
BsiSI CCGG 5 cut(s) 623, 791, 1175, 1388, 1986
BslFI GGGAC 1 cut(s) 1877
BslI CCNNNNNNNGG 3 cut(s) 1033, 1034, 1900
BsmAI GTCTC 2 cut(s) 1227, 2139
BsmFI GGGAC 1 cut(s) 1877
BsmI GAATGC 2 cut(s) 463, 496
BsnI GGCC 7 cut(s) 19, 29, 445, 454, 1712, 1869, 1954
Bso31I GGTCTC 1 cut(s) 2139
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 5 cut(s) 9, 104, 659, 2020, 2292
Bsp13I TCCGGA 1 cut(s) 1387
Bsp143I GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
Bsp19I CCATGG 1 cut(s) 864
BspACI CCGC 4 cut(s) 84, 494, 1003, 1329
BspANI GGCC 7 cut(s) 19, 29, 445, 454, 1712, 1869, 1954
BspCNI CTCAG 1 cut(s) 290
BspEI TCCGGA 1 cut(s) 1387
BspFNI CGCG 1 cut(s) 2325
BspLI GGNNCC 2 cut(s) 862, 2551
BspMAI CTGCAG 5 cut(s) 54, 772, 1510, 2392, 2599
BspPI GGATC 5 cut(s) 855, 868, 1578, 1721, 2571
BspQI GCTCTTC 3 cut(s) 14, 769, 1386
BspT104I TTCGAA 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 1414
BspTNI GGTCTC 1 cut(s) 2139
BsrDI GCAATG 2 cut(s) 1221, 2586
BsrI ACTGG 8 cut(s) 289, 1713, 2071, 2093, 2234, 2297, 2303, 2458
BssECI CCNNGG 6 cut(s) 318, 514, 828, 864, 1899, 2559
BssMI GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
BssT1I CCWWGG 3 cut(s) 828, 864, 1899
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 5 cut(s) 258, 745, 842, 920, 1301
Bst6I CTCTTC 7 cut(s) 14, 573, 656, 708, 769, 791, 1386
BstAFI CTTAAG 1 cut(s) 1414
BstAPI GCANNNNNTGC 2 cut(s) 1313, 2206
BstBAI YACGTR 1 cut(s) 1661
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 1616, 2358
BstDEI CTNAG 4 cut(s) 277, 1472, 1532, 2511
BstDSI CCRYGG 2 cut(s) 318, 864
BstF5I GGATG 8 cut(s) 676, 904, 943, 1594, 1858, 2005, 2394, 2618
BstFNI CGCG 1 cut(s) 2325
BstHHI GCGC 3 cut(s) 1126, 1980, 2325
BstKTI GATC 5 cut(s) 863, 1146, 1573, 1729, 2566
BstMAI GTCTC 2 cut(s) 1227, 2139
BstMBI GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
BstMWI GCNNNNNNNGC 9 cut(s) 58, 451, 460, 1211, 1313, 1769, 2054, 2206, 2441
BstNI CCWGG 1 cut(s) 896
BstNSI RCATGY 2 cut(s) 1369, 2248
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 5 cut(s) 50, 768, 1506, 2388, 2595
BstSLI GKGCMC 3 cut(s) 104, 2020, 2292
BstSNI TACGTA 1 cut(s) 1661
BstUI CGCG 1 cut(s) 2325
BstV1I GCAGC 6 cut(s) 64, 782, 1199, 1449, 2111, 2402
BstX2I RGATCY 2 cut(s) 860, 1143
BstYI RGATCY 2 cut(s) 860, 1143
Bsu36I CCTNAGG 1 cut(s) 2511
BsuI GTATCC 2 cut(s) 817, 862
BsuRI GGCC 7 cut(s) 19, 29, 445, 454, 1712, 1869, 1954
BtgI CCRYGG 2 cut(s) 318, 864
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 8 cut(s) 676, 904, 943, 1594, 1858, 2005, 2394, 2618
BtsI GCAGTG 2 cut(s) 47, 1851
BtsIMutI CAGTG 6 cut(s) 47, 847, 1851, 2018, 2290, 2471
Cac8I GCNNGC 2 cut(s) 1616, 2358
CaiI CAGNNNCTG 2 cut(s) 284, 2473
CfoI GCGC 3 cut(s) 1126, 1980, 2325
Cfr13I GGNCC 8 cut(s) 434, 443, 452, 892, 1301, 1832, 2231, 2550
CseI GACGC 1 cut(s) 620
Csp6I GTAC 6 cut(s) 247, 259, 733, 942, 1662, 2086
CspCI CAANNNNNGTGG 4 cut(s) 535, 570, 1678, 1713
CviQI GTAC 6 cut(s) 247, 259, 733, 942, 1662, 2086
DdeI CTNAG 4 cut(s) 277, 1472, 1532, 2511
DpnI GATC 5 cut(s) 862, 1145, 1572, 1728, 2565
DpnII GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 3 cut(s) 17, 1710, 1952
Eam1104I CTCTTC 7 cut(s) 14, 573, 656, 708, 769, 791, 1386
EarI CTCTTC 7 cut(s) 14, 573, 656, 708, 769, 791, 1386
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 7
Eco105I TACGTA 1 cut(s) 1661
Eco130I CCWWGG 3 cut(s) 828, 864, 1899
Eco147I AGGCCT 1 cut(s) 1869
Eco24I GRGCYC 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 2139
Eco47I GGWCC 6 cut(s) 434, 892, 1301, 1832, 2231, 2550
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 2421
Eco81I CCTNAGG 1 cut(s) 2511
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 892
EcoRI GAATTC 1 cut(s) 1092
EcoRII CCWGG 1 cut(s) 894
EcoT14I CCWWGG 3 cut(s) 828, 864, 1899
EcoT22I ATGCAT 1 cut(s) 2351
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 3 cut(s) 828, 864, 1899
FaqI GGGAC 1 cut(s) 1877
FauI CCCGC 1 cut(s) 1010
FblI GTMKAC 1 cut(s) 1502
Fnu4HI GCNGC 6 cut(s) 53, 771, 1188, 1463, 2100, 2391
FokI GGATG 8 cut(s) 683, 911, 950, 1601, 1865, 2012, 2381, 2625
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 6 cut(s) 53, 771, 1188, 1463, 2100, 2391
FspBI CTAG 7 cut(s) 197, 201, 393, 471, 852, 1206, 1284
GlaI GCGC 3 cut(s) 1125, 1979, 2324
GluI GCNGC 6 cut(s) 53, 771, 1188, 1463, 2100, 2391
HaeIII GGCC 7 cut(s) 19, 29, 445, 454, 1712, 1869, 1954
HapII CCGG 5 cut(s) 623, 791, 1175, 1388, 1986
HgaI GACGC 1 cut(s) 620
HhaI GCGC 3 cut(s) 1126, 1980, 2325
Hin6I GCGC 3 cut(s) 1124, 1978, 2323
HinP1I GCGC 3 cut(s) 1124, 1978, 2323
HindIII AAGCTT 2 cut(s) 1542, 1795
HpaII CCGG 5 cut(s) 623, 791, 1175, 1388, 1986
HphI GGTGA 6 cut(s) 28, 395, 1189, 1238, 1264, 1975
Hpy166II GTNNAC 3 cut(s) 261, 318, 1503
Hpy188III TCNNGA 7 cut(s) 279, 336, 366, 1388, 1548, 1597, 2524
Hpy8I GTNNAC 3 cut(s) 261, 318, 1503
HpyAV CCTTC 8 cut(s) 43, 147, 373, 579, 1058, 1569, 1950, 2279
HpyCH4III ACNGT 5 cut(s) 258, 745, 842, 920, 1301
HpyCH4IV ACGT 3 cut(s) 117, 245, 1660
HpyF10VI GCNNNNNNNGC 9 cut(s) 58, 451, 460, 1211, 1313, 1769, 2054, 2206, 2441
HpyF3I CTNAG 4 cut(s) 277, 1472, 1532, 2511
HpySE526I ACGT 3 cut(s) 117, 245, 1660
HspAI GCGC 3 cut(s) 1124, 1978, 2323
Kpn2I TCCGGA 1 cut(s) 1387
Kzo9I GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
LguI GCTCTTC 3 cut(s) 14, 769, 1386
LmnI GCTCC 5 cut(s) 4, 727, 1068, 1372, 1856
Lsp1109I GCAGC 6 cut(s) 64, 782, 1199, 1449, 2111, 2402
LweI GCATC 9 cut(s) 624, 1579, 1772, 1989, 2035, 2209, 2215, 2395, 2603
MaeI CTAG 7 cut(s) 197, 201, 393, 471, 852, 1206, 1284
MaeII ACGT 3 cut(s) 117, 245, 1660
MaeIII GTNAC 4 cut(s) 914, 1656, 2125, 2554
MalI GATC 5 cut(s) 862, 1145, 1572, 1728, 2565
MboI GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
MfeI CAATTG 1 cut(s) 1716
MflI RGATCY 2 cut(s) 860, 1143
MhlI GDGCHC 5 cut(s) 9, 104, 659, 2020, 2292
MlsI TGGCCA 2 cut(s) 1712, 1954
MluNI TGGCCA 2 cut(s) 1712, 1954
MlyI GAGTC 3 cut(s) 820, 1281, 1763
MmeI TCCRAC 5 cut(s) 37, 66, 705, 794, 1350
Mox20I TGGCCA 2 cut(s) 1712, 1954
Mph1103I ATGCAT 1 cut(s) 2351
MroI TCCGGA 1 cut(s) 1387
MroXI GAANNNNTTC 1 cut(s) 292
MscI TGGCCA 2 cut(s) 1712, 1954
MseI TTAA 8 cut(s) 123, 177, 311, 630, 720, 1415, 1514, 1917
MslI CAYNNNNRTG 1 cut(s) 2344
Msp20I TGGCCA 2 cut(s) 1712, 1954
MspA1I CMGCKG 1 cut(s) 773
MspCI CTTAAG 1 cut(s) 1414
MspI CCGG 5 cut(s) 623, 791, 1175, 1388, 1986
MspR9I CCNGG 1 cut(s) 896
MunI CAATTG 1 cut(s) 1716
Mva1269I GAATGC 2 cut(s) 463, 496
MvaI CCWGG 1 cut(s) 896
MvnI CGCG 1 cut(s) 2325
MwoI GCNNNNNNNGC 9 cut(s) 58, 451, 460, 1211, 1313, 1769, 2054, 2206, 2441
NcoI CCATGG 1 cut(s) 864
NdeII GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
NlaIV GGNNCC 2 cut(s) 862, 2551
NmeAIII GCCGAG 2 cut(s) 539, 1777
NmuCI GTSAC 2 cut(s) 2125, 2554
NsiI ATGCAT 1 cut(s) 2351
NspI RCATGY 2 cut(s) 1369, 2248
NspV TTCGAA 1 cut(s) 506
PceI AGGCCT 1 cut(s) 1869
PciI ACATGT 1 cut(s) 1365
PciSI GCTCTTC 3 cut(s) 14, 769, 1386
PctI GAATGC 2 cut(s) 463, 496
PdmI GAANNNNTTC 1 cut(s) 292
PfeI GAWTC 7 cut(s) 833, 932, 1046, 2276, 2327, 2384, 2462
PkrI GCNGC 6 cut(s) 54, 772, 1189, 1464, 2101, 2392
PleI GAGTC 3 cut(s) 819, 1281, 1762
PpsI GAGTC 3 cut(s) 819, 1281, 1762
Ppu21I YACGTR 1 cut(s) 1661
PpuMI RGGWCCY 1 cut(s) 892
PscI ACATGT 1 cut(s) 1365
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 892
Psp6I CCWGG 1 cut(s) 894
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 2 cut(s) 862, 2551
PspPI GGNCC 8 cut(s) 434, 443, 452, 892, 1301, 1832, 2231, 2550
PspPPI RGGWCCY 1 cut(s) 892
PstI CTGCAG 5 cut(s) 54, 772, 1510, 2392, 2599
PstNI CAGNNNCTG 2 cut(s) 284, 2473
PsuI RGATCY 2 cut(s) 860, 1143
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 6 cut(s) 248, 260, 734, 943, 1663, 2087
RsaNI GTAC 6 cut(s) 247, 259, 733, 942, 1662, 2086
RseI CAYNNNNRTG 1 cut(s) 2344
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 3 cut(s) 14, 769, 1386
SaqAI TTAA 8 cut(s) 123, 177, 311, 630, 720, 1415, 1514, 1917
SatI GCNGC 6 cut(s) 53, 771, 1188, 1463, 2100, 2391
Sau3AI GATC 5 cut(s) 860, 1143, 1570, 1726, 2563
Sau96I GGNCC 8 cut(s) 434, 443, 452, 892, 1301, 1832, 2231, 2550
SchI GAGTC 3 cut(s) 820, 1281, 1763
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 5 cut(s) 9, 104, 659, 2020, 2292
SfaNI GCATC 9 cut(s) 624, 1579, 1772, 1989, 2035, 2209, 2215, 2395, 2603
SfcI CTRYAG 5 cut(s) 50, 768, 1506, 2388, 2595
SfiI GGCCNNNNNGGCC 1 cut(s) 451
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 6 cut(s) 434, 892, 1301, 1832, 2231, 2550
SmiMI CAYNNNNRTG 1 cut(s) 2344
SmlI CTYRAG 2 cut(s) 1414, 1792
SmoI CTYRAG 2 cut(s) 1414, 1792
SnaBI TACGTA 1 cut(s) 1661
SseBI AGGCCT 1 cut(s) 1869
SsiI CCGC 4 cut(s) 84, 494, 1003, 1329
SspI AATATT 1 cut(s) 2495
SspMI CTAG 7 cut(s) 197, 201, 393, 471, 852, 1206, 1284
SstI GAGCTC 1 cut(s) 9
StuI AGGCCT 1 cut(s) 1869
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 3 cut(s) 828, 864, 1899
TaaI ACNGT 5 cut(s) 258, 745, 842, 920, 1301
TaiI ACGT 3 cut(s) 120, 248, 1663
TaqI TCGA 7 cut(s) 239, 335, 506, 659, 1573, 1596, 2573
TfiI GAWTC 7 cut(s) 833, 932, 1046, 2276, 2327, 2384, 2462
Tru1I TTAA 8 cut(s) 123, 177, 311, 630, 720, 1415, 1514, 1917
Tru9I TTAA 8 cut(s) 123, 177, 311, 630, 720, 1415, 1514, 1917
TscAI CASTG 6 cut(s) 54, 847, 1851, 2025, 2297, 2478
TseFI GTSAC 2 cut(s) 2125, 2554
TseI GCWGC 6 cut(s) 52, 770, 1187, 1462, 2099, 2390
Tsp45I GTSAC 2 cut(s) 2125, 2554
TspDTI ATGAA 3 cut(s) 966, 1277, 1958
TspGWI ACGGA 6 cut(s) 119, 335, 725, 1280, 2442, 2542
TspRI CASTG 6 cut(s) 54, 847, 1851, 2025, 2297, 2478
Vha464I CTTAAG 1 cut(s) 1414
VpaK11BI GGWCC 6 cut(s) 434, 892, 1301, 1832, 2231, 2550
XapI RAATTY 8 cut(s) 125, 877, 961, 997, 1092, 1805, 1886, 2516
XceI RCATGY 2 cut(s) 1369, 2248
XmiI GTMKAC 1 cut(s) 1502
XmnI GAANNNNTTC 1 cut(s) 292
XspI CTAG 7 cut(s) 197, 201, 393, 471, 852, 1206, 1284
Zsp2I ATGCAT 1 cut(s) 2351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.