Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

3536782 .. 3544435

Gene Structure 7654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1043200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGGCGAAGGTCAAGGTCACCTTGGAGGTCGGCAACGATGGCGTTGCAGTCATCACCATCTTCAATCCACCTGTCAACGCCTTGGACATACCGACTGTAGCTAGCTTGAAGGAGAAATTCGAGGAGGCCGTGAGGCGAAACGACGTCAAGGCGATCGTTTTGACTGGCAATGCGGGAAGGTTTTCAGCCGGTTTCGATATCAATGTTTTCGGGAAGGTTCACGAGTCTGGGGATGTTTTGGTTATGCCCGATGTATCTTTTGACTTGGTGCTCAACACACTTGAAAATTCAAAAAAGCCTGTTGTTGCTGCCGTCGAAGGACTTGCACTAGGAGGTGGCTTGGAGTTGGCATTGGGGTGCCATGCACGTATTGCTGCTCCAAGAGCTCAACTTAGTCTTCCAGAGTTGTCTCTTGGAGTAATACCTGGATTTGGAGGCACACAACGTCTTCCAAGGCTTGTAGGGCTTCAGAAGGCAATTGAAATGATGCTGTCGTTGAAGCCAATCCTGTCTGAAGAAGGGAAGAAGCTTGGTCTTATAGATGCTATTGTGTCTCCTCAAGAGTTGCTGAAAGTATCTCGTTCATGGGCTTTGGAAATTGCAGACAGACGAAAACCGTGGTTGCGCTCTCTTCACAGGACAGATAAACTTGCTTCCCTGTCTGAGGCTCGAGAGATACTGAAAGTTGCCAGACAACAAGCCAGGAAGAATGCTCCCAATATGCCTCATCACCAAGCATGCCTCAATGTGATCGAGGATGGCATTGTTCATGGAGGATACAGTGGCCTTTTAATGGAGGCTAAAGTTTCTAAGGAGTTACTTCTAGCAGACACTGCAAGAGGTCTTGTCCATGTTTTTTTTGCCCAACGCGCAACATCAAAGGTGCCCAATGTTACTGATGTTGGCCTTAAACCAAGGCAAATAAAGAAAGTTGCTGTTATTGGGGGAGGTCTGATGGGTTCTGGCATTGCTACAGCCCTTCTCCTCAGCAATATATCTGTGGTTCTAAAGGAAGTCAACTTTGAATATCTTCAGAAGGGCATAGAAAAGATAGAAGGAAACGTTAAAGGCTTAGTAACTAGAAGGGGGCTGACACAGGACAAGGCACAGAAAGCTCTCGCATTGCTTAAAGATAGTTTGGACTACTCCGACTTCAAAGATGTGGATATGGTCATAGAGGCCGTCATTGAAAGTGTTCCTCTCAAACAAAAAATCTTTAGTGAACTCGAGAATGTTTGTCCTCCTCACTGCGTATTGGCTACAAACACATCCACTATTGACCTCAACGTTGTTGGAGAAAAGACAAACTCTCATGATCGCATTGTGGGGGCACATTTTTTCAGCCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCAGCACAAGTTATTCTTGATCTTTTTACTGTTGGAAAAATAATAAAGAAAGTTCCTGTTGTGGTTGGTAACTGCACAGGCTTTGCCGCCAATCGAGCATTCTTTCCCTACTCACAAAGTGCCCATTTATTAGTCAATTCAGGAGTGGATGTGTTCAGAATTGACAGGATCATCAGGGATTTTGGCCTACCTATGGGTCCATTCCAGCTTCAAGACTTGGGAGGCTACGGAGTAGCCCTTGCTGCTGGAAAGGAATTATCTCGTGCTTTCCCTGATCGCACATTTAAATCTCCATTGGTTGAGATCTTGATAAAAAATGGACGAAATGGTAAAAACAACGGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

580

Amino Acids

62.57

Weight (kDa)

9.33

Isoelectric Point (pI)

31.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	12 - 212	4.5e-42	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 194	5.5e-21	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	310 - 488	5.9e-57	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	491 - 579	9.5e-18	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	147
AccB1I	GGYRCC	2 cut(s)	357, 883
AccII	CGCG	1 cut(s)	870
AciI	CCGC	2 cut(s)	173, 1479
AclI	AACGTT	2 cut(s)	1062, 1287
AclWI	GGATC	1 cut(s)	1568
AcsI	RAATTY	2 cut(s)	116, 286
AcuI	CTGAAG	4 cut(s)	452, 534, 1016, 1377
AcyI	GRCGYC	1 cut(s)	144
AdeI	CACNNNGTG	1 cut(s)	1511
AfaI	GTAC	1 cut(s)	1377
AfiI	CCNNNNNNNGG	4 cut(s)	431, 664, 793, 1585
AjnI	CCWGG	2 cut(s)	424, 701
AjuI	GAANNNNNNNTTGG	2 cut(s)	1475, 1507
AluBI	AGCT	6 cut(s)	101, 105, 386, 529, 1115, 1600
AluI	AGCT	6 cut(s)	101, 105, 386, 529, 1115, 1600
Alw21I	GWGCWC	2 cut(s)	273, 388
Alw26I	GTCTC	2 cut(s)	414, 558
AlwI	GGATC	1 cut(s)	1568
AlwNI	CAGNNNCTG	1 cut(s)	833
Ama87I	CYCGRG	2 cut(s)	669, 1226
AoxI	GGCC	5 cut(s)	126, 784, 904, 1179, 1576
ApeKI	GCWGC	3 cut(s)	308, 374, 1634
ApoI	RAATTY	2 cut(s)	116, 286
Asp700I	GAANNNNTTC	3 cut(s)	181, 1029, 1194
AspLEI	GCGC	2 cut(s)	627, 872
AspS9I	GGNCC	1 cut(s)	1589
AsuHPI	GGTGA	3 cut(s)	10, 46, 722
AsuNHI	GCTAGC	1 cut(s)	101
AvaI	CYCGRG	2 cut(s)	669, 1226
AvaII	GGWCC	1 cut(s)	1589
BaeGI	GKGCMC	3 cut(s)	888, 1333, 1516
BanI	GGYRCC	2 cut(s)	357, 883
BanII	GRGCYC	1 cut(s)	388
BauI	CACGAG	2 cut(s)	221, 1653
BbsI	GAAGAC	3 cut(s)	389, 440, 1394
Bbv12I	GWGCWC	2 cut(s)	273, 388
BbvCI	CCTCAGC	1 cut(s)	986
BbvI	GCAGC	3 cut(s)	295, 361, 1621
BccI	CCATC	4 cut(s)	32, 65, 752, 949
BceAI	ACGGC	3 cut(s)	113, 296, 1166
BcgI	CGANNNNNNTGC	4 cut(s)	26, 60, 305, 339
BciT130I	CCWGG	2 cut(s)	426, 703
BciVI	GTATCC	1 cut(s)	770
BcoDI	GTCTC	2 cut(s)	414, 558
BfaI	CTAG	4 cut(s)	102, 329, 824, 1080
BfmI	CTRYAG	2 cut(s)	96, 972
BfuI	GTATCC	1 cut(s)	770
BglII	AGATCT	1 cut(s)	1695
BisI	GCNGC	4 cut(s)	309, 375, 1479, 1635
BlsI	GCNGC	4 cut(s)	310, 376, 1480, 1636
Bme1390I	CCNGG	2 cut(s)	426, 703
Bme18I	GGWCC	1 cut(s)	1589
BmeT110I	CYCGRG	2 cut(s)	669, 1226
BmgT120I	GGNCC	1 cut(s)	1589
BmiI	GGNNCC	3 cut(s)	359, 885, 1590
BmrFI	CCNGG	2 cut(s)	426, 703
BmsI	GCATC	3 cut(s)	477, 532, 1347
BmtI	GCTAGC	1 cut(s)	105
BpiI	GAAGAC	3 cut(s)	389, 440, 1394
Bpu10I	CCTNAGC	1 cut(s)	986
BpuEI	CTTGAG	1 cut(s)	543
BsaAI	YACGTR	1 cut(s)	368
BsaHI	GRCGYC	1 cut(s)	144
BsaJI	CCNNGG	5 cut(s)	21, 81, 452, 617, 914
BsaXI	ACNNNNNCTCC	2 cut(s)	1527, 1557
Bsc4I	CCNNNNNNNGG	4 cut(s)	431, 664, 793, 1585
Bse118I	RCCGGY	1 cut(s)	188
Bse1I	ACTGG	1 cut(s)	169
Bse3DI	GCAATG	3 cut(s)	175, 966, 1121
BseBI	CCWGG	2 cut(s)	426, 703
BseDI	CCNNGG	5 cut(s)	21, 81, 452, 617, 914
BseGI	GGATG	4 cut(s)	238, 763, 1268, 1546
BseLI	CCNNNNNNNGG	4 cut(s)	431, 664, 793, 1585
BseMI	GCAATG	3 cut(s)	175, 966, 1121
BseMII	CTCAG	2 cut(s)	654, 1000
BseNI	ACTGG	1 cut(s)	169
BseRI	GAGGAG	4 cut(s)	137, 546, 974, 1233
BseSI	GKGCMC	3 cut(s)	888, 1333, 1516
BseXI	GCAGC	3 cut(s)	295, 361, 1621
BsgI	GTGCAG	1 cut(s)	1450
Bsh1236I	CGCG	1 cut(s)	870
Bsh1285I	CGRYCG	1 cut(s)	156
BshFI	GGCC	5 cut(s)	128, 786, 906, 1181, 1578
BshNI	GGYRCC	2 cut(s)	357, 883
BsiEI	CGRYCG	1 cut(s)	156
BsiHKAI	GWGCWC	2 cut(s)	273, 388
BsiHKCI	CYCGRG	2 cut(s)	669, 1226
BsiSI	CCGG	1 cut(s)	189
BslI	CCNNNNNNNGG	4 cut(s)	431, 664, 793, 1585
BsmAI	GTCTC	2 cut(s)	414, 558
BsmI	GAATGC	2 cut(s)	715, 1490
BsnI	GGCC	5 cut(s)	128, 786, 906, 1181, 1578
BsoBI	CYCGRG	2 cut(s)	669, 1226
Bsp1286I	GDGCHC	5 cut(s)	273, 388, 888, 1333, 1516
Bsp143I	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
BspACI	CCGC	2 cut(s)	173, 1479
BspANI	GGCC	5 cut(s)	128, 786, 906, 1181, 1578
BspCNI	CTCAG	2 cut(s)	655, 999
BspFNI	CGCG	1 cut(s)	870
BspHI	TCATGA	1 cut(s)	1312
BspLI	GGNNCC	3 cut(s)	359, 885, 1590
BspOI	GCTAGC	1 cut(s)	105
BspPI	GGATC	1 cut(s)	1568
BspT107I	GGYRCC	2 cut(s)	357, 883
BsrDI	GCAATG	3 cut(s)	175, 966, 1121
BsrFI	RCCGGY	1 cut(s)	188
BsrI	ACTGG	1 cut(s)	169
BssAI	RCCGGY	1 cut(s)	188
BssECI	CCNNGG	5 cut(s)	21, 81, 452, 617, 914
BssMI	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	2 cut(s)	221, 1653
BssT1I	CCWWGG	4 cut(s)	21, 81, 452, 914
Bst2BI	CACGAG	2 cut(s)	221, 1653
Bst2UI	CCWGG	2 cut(s)	426, 703
Bst4CI	ACNGT	4 cut(s)	97, 618, 782, 1423
Bst6I	CTCTTC	2 cut(s)	636, 1368
BstACI	GRCGYC	1 cut(s)	144
BstAPI	GCANNNNNTGC	2 cut(s)	371, 833
BstBAI	YACGTR	1 cut(s)	368
BstC8I	GCNNGC	2 cut(s)	103, 739
BstDEI	CTNAG	5 cut(s)	392, 663, 810, 986, 1072
BstDSI	CCRYGG	1 cut(s)	617
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	1 cut(s)	662
BstF5I	GGATG	4 cut(s)	238, 763, 1268, 1546
BstFNI	CGCG	1 cut(s)	870
BstHHI	GCGC	2 cut(s)	627, 872
BstKTI	GATC	7 cut(s)	156, 753, 1318, 1414, 1563, 1669, 1698
BstMAI	GTCTC	2 cut(s)	414, 558
BstMBI	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
BstMCI	CGRYCG	1 cut(s)	156
BstMWI	GCNNNNNNNGC	9 cut(s)	39, 371, 383, 463, 833, 869, 1112, 1487, 1634
BstNI	CCWGG	2 cut(s)	426, 703
BstNSI	RCATGY	1 cut(s)	741
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	2 cut(s)	424, 701
BstSFI	CTRYAG	2 cut(s)	96, 972
BstSLI	GKGCMC	3 cut(s)	888, 1333, 1516
BstUI	CGCG	1 cut(s)	870
BstV1I	GCAGC	3 cut(s)	295, 361, 1621
BstV2I	GAAGAC	3 cut(s)	389, 440, 1394
BstX2I	RGATCY	1 cut(s)	1695
BstYI	RGATCY	1 cut(s)	1695
BsuI	GTATCC	1 cut(s)	770
BsuRI	GGCC	5 cut(s)	128, 786, 906, 1181, 1578
BtgI	CCRYGG	1 cut(s)	617
BtsCI	GGATG	4 cut(s)	238, 763, 1268, 1546
BtsI	GCAGTG	2 cut(s)	831, 1246
BtsIMutI	CAGTG	3 cut(s)	787, 831, 1246
Cac8I	GCNNGC	2 cut(s)	103, 739
CaiI	CAGNNNCTG	1 cut(s)	833
CciI	TCATGA	1 cut(s)	1312
CfoI	GCGC	2 cut(s)	627, 872
Cfr10I	RCCGGY	1 cut(s)	188
Cfr13I	GGNCC	1 cut(s)	1589
Csp6I	GTAC	1 cut(s)	1376
CviAII	CATG	7 cut(s)	362, 585, 738, 770, 851, 1313, 1352
CviQI	GTAC	1 cut(s)	1376
DdeI	CTNAG	5 cut(s)	392, 663, 810, 986, 1072
DpnI	GATC	7 cut(s)	155, 752, 1317, 1413, 1562, 1668, 1697
DpnII	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
DraI	TTTAAA	1 cut(s)	1678
DraIII	CACNNNGTG	1 cut(s)	1511
Eam1104I	CTCTTC	2 cut(s)	636, 1368
EarI	CTCTTC	2 cut(s)	636, 1368
Ecl136II	GAGCTC	1 cut(s)	386
Eco130I	CCWWGG	4 cut(s)	21, 81, 452, 914
Eco24I	GRGCYC	1 cut(s)	388
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	1 cut(s)	1589
Eco53kI	GAGCTC	1 cut(s)	386
Eco57I	CTGAAG	4 cut(s)	452, 534, 1016, 1377
Eco88I	CYCGRG	2 cut(s)	669, 1226
Eco91I	GGTNACC	1 cut(s)	16
EcoICRI	GAGCTC	1 cut(s)	386
EcoNI	CCTNNNNNAGG	1 cut(s)	662
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	2 cut(s)	424, 701
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	4 cut(s)	21, 81, 452, 914
EcoT38I	GRGCYC	1 cut(s)	388
ErhI	CCWWGG	4 cut(s)	21, 81, 452, 914
FaeI	CATG	7 cut(s)	365, 588, 741, 773, 854, 1316, 1355
FalI	AAGNNNNNCTT	3 cut(s)	37, 1392, 1424
FatI	CATG	7 cut(s)	361, 584, 737, 769, 850, 1312, 1351
FauI	CCCGC	1 cut(s)	166
Fnu4HI	GCNGC	4 cut(s)	309, 375, 1479, 1635
FokI	GGATG	4 cut(s)	245, 770, 1255, 1553
FriOI	GRGCYC	1 cut(s)	388
Fsp4HI	GCNGC	4 cut(s)	309, 375, 1479, 1635
FspBI	CTAG	4 cut(s)	102, 329, 824, 1080
GlaI	GCGC	2 cut(s)	626, 871
GluI	GCNGC	4 cut(s)	309, 375, 1479, 1635
HaeIII	GGCC	5 cut(s)	128, 786, 906, 1181, 1578
HapII	CCGG	1 cut(s)	189
HhaI	GCGC	2 cut(s)	627, 872
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	7 cut(s)	365, 588, 741, 773, 854, 1316, 1355
Hin6I	GCGC	2 cut(s)	625, 870
HinP1I	GCGC	2 cut(s)	625, 870
HincII	GTYRAC	2 cut(s)	76, 1018
HindII	GTYRAC	2 cut(s)	76, 1018
HindIII	AAGCTT	1 cut(s)	527
HinfI	GANTC	1 cut(s)	224
HpaII	CCGG	1 cut(s)	189
HphI	GGTGA	3 cut(s)	10, 46, 722
Hpy166II	GTNNAC	4 cut(s)	76, 220, 1018, 1223
Hpy188I	TCNGA	7 cut(s)	471, 514, 664, 954, 1035, 1150, 1550
Hpy8I	GTNNAC	4 cut(s)	76, 220, 1018, 1223
Hpy99I	CGWCG	2 cut(s)	146, 317
HpyCH4III	ACNGT	4 cut(s)	97, 618, 782, 1423
HpyCH4IV	ACGT	5 cut(s)	144, 367, 445, 1062, 1287
HpyCH4V	TGCA	6 cut(s)	47, 326, 365, 602, 836, 1467
HpyF10VI	GCNNNNNNNGC	9 cut(s)	39, 371, 383, 463, 833, 869, 1112, 1487, 1634
HpyF3I	CTNAG	5 cut(s)	392, 663, 810, 986, 1072
HpySE526I	ACGT	5 cut(s)	144, 367, 445, 1062, 1287
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	7 cut(s)	365, 588, 741, 773, 854, 1316, 1355
HspAI	GCGC	2 cut(s)	625, 870
Kzo9I	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
LmnI	GCTCC	2 cut(s)	382, 718
Lsp1109I	GCAGC	3 cut(s)	295, 361, 1621
LweI	GCATC	3 cut(s)	477, 532, 1347
MaeI	CTAG	4 cut(s)	102, 329, 824, 1080
MaeII	ACGT	5 cut(s)	144, 367, 445, 1062, 1287
MaeIII	GTNAC	5 cut(s)	16, 816, 892, 1075, 1460
MalI	GATC	7 cut(s)	155, 752, 1317, 1413, 1562, 1668, 1697
MboI	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
MfeI	CAATTG	1 cut(s)	477
MflI	RGATCY	1 cut(s)	1695
MhlI	GDGCHC	5 cut(s)	273, 388, 888, 1333, 1516
MluCI	AATT	7 cut(s)	116, 286, 477, 597, 1528, 1551, 1646
MlyI	GAGTC	1 cut(s)	233
MmeI	TCCRAC	3 cut(s)	1173, 1273, 1405
MroXI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
MseI	TTAA	5 cut(s)	791, 909, 1065, 1128, 1677
MslI	CAYNNNNRTG	1 cut(s)	355
MspI	CCGG	1 cut(s)	189
MspR9I	CCNGG	2 cut(s)	426, 703
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	2 cut(s)	715, 1490
MvaI	CCWGG	2 cut(s)	426, 703
MvnI	CGCG	1 cut(s)	870
MwoI	GCNNNNNNNGC	9 cut(s)	39, 371, 383, 463, 833, 869, 1112, 1487, 1634
NdeII	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
NheI	GCTAGC	1 cut(s)	101
NlaIII	CATG	7 cut(s)	365, 588, 741, 773, 854, 1316, 1355
NlaIV	GGNNCC	3 cut(s)	359, 885, 1590
NmuCI	GTSAC	1 cut(s)	16
NspI	RCATGY	1 cut(s)	741
PaeI	GCATGC	1 cut(s)	741
PaeR7I	CTCGAG	2 cut(s)	669, 1226
PagI	TCATGA	1 cut(s)	1312
PcsI	WCGNNNNNNNCGW	1 cut(s)	126
PctI	GAATGC	2 cut(s)	715, 1490
PdmI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
PkrI	GCNGC	4 cut(s)	310, 376, 1480, 1636
Ple19I	CGATCG	1 cut(s)	156
PleI	GAGTC	1 cut(s)	232
PpsI	GAGTC	1 cut(s)	232
Ppu21I	YACGTR	1 cut(s)	368
Psp124BI	GAGCTC	1 cut(s)	388
Psp1406I	AACGTT	2 cut(s)	1062, 1287
Psp6I	CCWGG	2 cut(s)	424, 701
PspEI	GGTNACC	1 cut(s)	16
PspGI	CCWGG	2 cut(s)	424, 701
PspN4I	GGNNCC	3 cut(s)	359, 885, 1590
PspPI	GGNCC	1 cut(s)	1589
PstNI	CAGNNNCTG	1 cut(s)	833
PsuI	RGATCY	1 cut(s)	1695
PvuI	CGATCG	1 cut(s)	156
RsaI	GTAC	1 cut(s)	1377
RsaNI	GTAC	1 cut(s)	1376
RseI	CAYNNNNRTG	1 cut(s)	355
SacI	GAGCTC	1 cut(s)	388
SaqAI	TTAA	5 cut(s)	791, 909, 1065, 1128, 1677
SatI	GCNGC	4 cut(s)	309, 375, 1479, 1635
Sau3AI	GATC	7 cut(s)	153, 750, 1315, 1411, 1560, 1666, 1695
Sau96I	GGNCC	1 cut(s)	1589
SchI	GAGTC	1 cut(s)	233
ScrFI	CCNGG	2 cut(s)	426, 703
SduI	GDGCHC	5 cut(s)	273, 388, 888, 1333, 1516
SfaNI	GCATC	3 cut(s)	477, 532, 1347
SfcI	CTRYAG	2 cut(s)	96, 972
Sfr274I	CTCGAG	2 cut(s)	669, 1226
SinI	GGWCC	1 cut(s)	1589
SlaI	CTCGAG	2 cut(s)	669, 1226
SmiI	ATTTAAAT	1 cut(s)	1678
SmiMI	CAYNNNNRTG	1 cut(s)	355
SmlI	CTYRAG	3 cut(s)	558, 669, 1226
SmoI	CTYRAG	3 cut(s)	558, 669, 1226
SphI	GCATGC	1 cut(s)	741
Sse9I	AATT	7 cut(s)	116, 286, 477, 597, 1528, 1551, 1646
SsiI	CCGC	2 cut(s)	173, 1479
SspMI	CTAG	4 cut(s)	102, 329, 824, 1080
SstI	GAGCTC	1 cut(s)	388
StyD4I	CCNGG	2 cut(s)	424, 701
StyI	CCWWGG	4 cut(s)	21, 81, 452, 914
SwaI	ATTTAAAT	1 cut(s)	1678
TaaI	ACNGT	4 cut(s)	97, 618, 782, 1423
TaiI	ACGT	5 cut(s)	147, 370, 448, 1065, 1290
TaqI	TCGA	7 cut(s)	120, 195, 315, 670, 753, 1227, 1486
TasI	AATT	7 cut(s)	116, 286, 477, 597, 1528, 1551, 1646
TauI	GCSGC	1 cut(s)	1481
Tru1I	TTAA	5 cut(s)	791, 909, 1065, 1128, 1677
Tru9I	TTAA	5 cut(s)	791, 909, 1065, 1128, 1677
TscAI	CASTG	3 cut(s)	787, 838, 1253
TseFI	GTSAC	1 cut(s)	16
TseI	GCWGC	3 cut(s)	308, 374, 1634
Tsp45I	GTSAC	1 cut(s)	16
TspDTI	ATGAA	2 cut(s)	573, 758
TspGWI	ACGGA	2 cut(s)	1393, 1635
TspRI	CASTG	3 cut(s)	787, 838, 1253
VpaK11BI	GGWCC	1 cut(s)	1589
XagI	CCTNNNNNAGG	1 cut(s)	662
XapI	RAATTY	2 cut(s)	116, 286
XceI	RCATGY	1 cut(s)	741
XhoI	CTCGAG	2 cut(s)	669, 1226
XmnI	GAANNNNTTC	3 cut(s)	181, 1029, 1194
XspI	CTAG	4 cut(s)	102, 329, 824, 1080
ZraI	GACGTC	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.