Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

3842670 .. 3848347

Gene Structure 5678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G004070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2172 bp

ATGGCGGGCGTCAAGGTCACCTTGGAGGTCGGAAGCGACGGTGTCGCCGTCATCACCATGTTCAATCCTCCCGTCAACGCCTTGGCCATATCCATTCTCAAGGGGCTGAAGGAGAAGTTCGATGAGGCCGCGAGGAGAAACGACGTCAAGGCTATTGTCGTTACTGGAAATGGAGGAAGGTTTTCTGGAGGTTTTGATATCAATGTTTTCGAGAAGGTTCACGCTACCGGGGATGTTTCGCTCATGCCTGATGTATCTGTTGACTTGATGGTCAACACTGTTGAAGATTCAAAGAAGCCTGTTGTTGCTGCTGTGGAAGGACTTGCGCTCGGAGGTGGCTTGGAGTTGGCATTGGGATGCCATGCTCGCATTGCTGCTCCAAGAACTCAACTAGGCCTGCCAGAGTTGACACTTGGTGTTATTCCGGGATTTGGAGGCACACAGCGTCTTCCAAGGCTTGCTGGGCTTCCGAAGGCAGTTGAGATGATGCTGTTATCCAAGTCAATCATGTCTGAAGAAGGGGCAAAGCTTGGTCTTATCGATGCTATTGTGCCTTCTCAAGAGTTGCTGAAAGTATCTCGATTATGGGCTTTGGACATTGCAGCGGCGCGAAAACCATGGTTACGGTCCCTTCACAGGACAGACAAGCTTTGCTCCCTATCTGAGGCACGCGAGATAATTAAAGTTGCTAGACAACAAGCCAGAAAGACGGCTCCCAATATGCCTCAGCATCAAGTATGCCTCGATGCAATTGAAGAGGGCATTATTCATGGAGGATATAGTGGAGTTCTGAAGGAAGCTAAGGTTTTTAAGGAGTTAGTTGTATCAGACACTTCAAAAGGTCTTGTACATGTATTTTTTGCGCAACGGGCAACATCAAAGGTGCCCAAGGTTACTGATGTTGGGCTTAAACCAAGGCATATAAAGAAGGTTGCTATCATTGGGGGAGGTCTGATGGGTTCTGGCATAGCTACAGCCCTTCTTCTGAGCAATGTATCTGTTGTTCTCAAAGAAATCAACTCTGAATTTCTTCAGAAGGGTGTACGAACAATAGAAGGAAATGTCAAAGGCTTGGTAACTAGAGGGAAGCTGGCACGGGATAAGGCAGACAAAGCTCTCTCATTGCTTAAAGGTAGTCTGGACTACTCAGACTTCAAAGATGTGGATATGGTCATAGAGGCTGTCATTGAGAGCGTTCCTCTCAAACAAAAGATTTTTGGTGAACTTGAGAAAGTTTGCCCTTCTCGTTGCATTTTGGCTACAAACACATCCACTATTGACCTCAATATAGTTGGAGAAAAGACGAGATCTCAGGATCGCATCGTCGGGGCACACTTTTTCAGTCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCGGCACAAGTAATTCTTGACCTTTTGACGGTTGGAAAAGTAATAAAGAAAGTTCCTGTTGTGGTTGGTAACTGTACGGGATTTGCAGTCAATCGGGCATTCTTTCCCTATACCCAAGGCGCACATATATTGGTCAATTCTGGTGTCGATGTGTTCAGGATTGACAGGATCATCAGCAATTTTGGCCTTCCTATGGGTCCATTCCAACTTCAGGATTTGGCAGGCTATGGAGTAGCTCTTGCTGTTGGAAAGGAATTTGCTAGTGCTTTCCCTGATCGCACATTTCAATCACCATTGGTCGAACTCTTAGTCAAAAATGGACGAAATGGTAAAAACAATGGAAAAGGATATTACACTTATGAAAAGGGAAGCAAGCCCAAGCCTGATCTGTCTGTGCTACCAATTATTGAGGAGTCTAGACGAATTACCAATATGATGCCTGGTGGAAAGCCTTTATCTGTTACAGACCAAGAAATTCTGGAGATAATACTCTTTCCTGTGGTGAATGAGGCATGTCGTATTTTGGATGAAGGAGTAGTTATTCGAGCAGCAGACCTTGACATTGCATCTGTTCTTGGCATGAGTTTCCCAAATTACCGGGGTGGCATTGTCTTTTGGGCAGATATGGTTGGGCCTAAGCATATATACGCTACTCTCAAGAAATGGTCAGGAGTATACGGTGGTTTCTTCAAACCATCAAGGTTTTTGGAAGAAAGAGCAACAAAAGGCTTGCCTCTGAGTGCACCCGCATCATCCTCATCTTCAAGGTCACGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

723

Amino Acids

77.87

Weight (kDa)

9.4

Isoelectric Point (pI)

34.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	13 - 211	2.2e-39	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	25 - 200	8.6e-23	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	310 - 488	1.6e-57	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	491 - 584	4.3e-22	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	269
AatII	GACGTC	1 cut(s)	147
Acc16I	TGCGCA	1 cut(s)	864
AccB1I	GGYRCC	1 cut(s)	883
AccI	GTMKAC	1 cut(s)	2067
AccII	CGCG	3 cut(s)	131, 610, 672
AciI	CCGC	4 cut(s)	5, 129, 605, 2139
AclWI	GGATC	2 cut(s)	1323, 1568
AcoI	YGGCCR	1 cut(s)	84
AcsI	RAATTY	3 cut(s)	1025, 1646, 1866
AcuI	CTGAAG	5 cut(s)	128, 534, 812, 1016, 1586
AcyI	GRCGYC	2 cut(s)	9, 144
AdeI	CACNNNGTG	1 cut(s)	416
AfaI	GTAC	4 cut(s)	849, 1044, 1377, 1468
AfiI	CCNNNNNNNGG	6 cut(s)	431, 636, 664, 1420, 1585, 1603
AflIII	ACRYGT	1 cut(s)	850
AjnI	CCWGG	1 cut(s)	1831
AluBI	AGCT	7 cut(s)	529, 649, 800, 971, 1090, 1115, 1628
AluI	AGCT	7 cut(s)	529, 649, 800, 971, 1090, 1115, 1628
Alw21I	GWGCWC	1 cut(s)	2137
Alw44I	GTGCAC	1 cut(s)	2133
AlwI	GGATC	2 cut(s)	1323, 1568
AoxI	GGCC	5 cut(s)	84, 126, 394, 1576, 2024
ApaLI	GTGCAC	1 cut(s)	2133
ApeKI	GCWGC	4 cut(s)	308, 374, 602, 1940
ApoI	RAATTY	3 cut(s)	1025, 1646, 1866
ArsI	GACNNNNNNTTYG	2 cut(s)	1686, 1718
Asp700I	GAANNNNTTC	2 cut(s)	181, 1029
AspLEI	GCGC	4 cut(s)	328, 610, 865, 1514
AspS9I	GGNCC	3 cut(s)	627, 1589, 2024
AsuC2I	CCSGG	3 cut(s)	229, 426, 1991
AsuHPI	GGTGA	5 cut(s)	10, 46, 1232, 1674, 1906
AvaII	GGWCC	2 cut(s)	627, 1589
BaeGI	GKGCMC	3 cut(s)	888, 1333, 2137
BalI	TGGCCA	1 cut(s)	86
BanI	GGYRCC	1 cut(s)	883
BbsI	GAAGAC	2 cut(s)	440, 1394
Bbv12I	GWGCWC	1 cut(s)	2137
BbvCI	CCTCAGC	1 cut(s)	726
BbvI	GCAGC	4 cut(s)	295, 361, 614, 1952
BccI	CCATC	3 cut(s)	262, 949, 2095
BceAI	ACGGC	2 cut(s)	32, 726
BciT130I	CCWGG	1 cut(s)	1833
BcnI	CCSGG	3 cut(s)	229, 426, 1991
BfaI	CTAG	5 cut(s)	392, 690, 1080, 1653, 1809
BfmI	CTRYAG	1 cut(s)	972
BglII	AGATCT	1 cut(s)	1307
BisI	GCNGC	6 cut(s)	129, 309, 375, 603, 606, 1941
BlsI	GCNGC	6 cut(s)	130, 310, 376, 604, 607, 1942
Bme1390I	CCNGG	4 cut(s)	229, 426, 1833, 1991
Bme18I	GGWCC	2 cut(s)	627, 1589
BmgT120I	GGNCC	3 cut(s)	627, 1589, 2024
BmiI	GGNNCC	4 cut(s)	629, 714, 885, 1590
BmrFI	CCNGG	4 cut(s)	229, 426, 1833, 1991
BpiI	GAAGAC	2 cut(s)	440, 1394
BplI	GAGNNNNNCTC	2 cut(s)	1183, 1215
BpmI	CTGGAG	2 cut(s)	207, 1892
Bpu10I	CCTNAGC	3 cut(s)	726, 801, 2028
BpuEI	CTTGAG	4 cut(s)	83, 543, 1247, 2033
BpuMI	CCSGG	3 cut(s)	229, 426, 1991
Bsa29I	ATCGAT	1 cut(s)	540
BsaHI	GRCGYC	2 cut(s)	9, 144
BsaJI	CCNNGG	9 cut(s)	21, 81, 228, 452, 617, 888, 914, 1507, 1990
Bsc4I	CCNNNNNNNGG	6 cut(s)	431, 636, 664, 1420, 1585, 1603
Bse1I	ACTGG	1 cut(s)	169
Bse3DI	GCAATG	5 cut(s)	369, 597, 997, 1121, 1953
BseBI	CCWGG	1 cut(s)	1833
BseCI	ATCGAT	1 cut(s)	540
BseDI	CCNNGG	9 cut(s)	21, 81, 228, 452, 617, 888, 914, 1507, 1990
BseGI	GGATG	5 cut(s)	238, 362, 1268, 1924, 2144
BseLI	CCNNNNNNNGG	6 cut(s)	431, 636, 664, 1420, 1585, 1603
BseMI	GCAATG	5 cut(s)	369, 597, 997, 1121, 1953
BseMII	CTCAG	6 cut(s)	654, 740, 977, 1161, 1325, 2120
BseNI	ACTGG	1 cut(s)	169
BseRI	GAGGAG	2 cut(s)	148, 1817
BseSI	GKGCMC	3 cut(s)	888, 1333, 2137
BseXI	GCAGC	4 cut(s)	295, 361, 614, 1952
BseYI	CCCAGC	1 cut(s)	461
Bsh1236I	CGCG	3 cut(s)	131, 610, 672
BshFI	GGCC	5 cut(s)	86, 128, 396, 1578, 2026
BshNI	GGYRCC	1 cut(s)	883
BshVI	ATCGAT	1 cut(s)	540
BsiHKAI	GWGCWC	1 cut(s)	2137
BsiSI	CCGG	3 cut(s)	228, 425, 1990
BslFI	GGGAC	1 cut(s)	613
BslI	CCNNNNNNNGG	6 cut(s)	431, 636, 664, 1420, 1585, 1603
BsmFI	GGGAC	1 cut(s)	613
BsmI	GAATGC	1 cut(s)	1490
BsnI	GGCC	5 cut(s)	86, 128, 396, 1578, 2026
Bsp1286I	GDGCHC	3 cut(s)	888, 1333, 2137
Bsp1407I	TGTACA	1 cut(s)	847
Bsp143I	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
Bsp19I	CCATGG	1 cut(s)	617
BspACI	CCGC	4 cut(s)	5, 129, 605, 2139
BspANI	GGCC	5 cut(s)	86, 128, 396, 1578, 2026
BspCNI	CTCAG	6 cut(s)	655, 739, 978, 1160, 1324, 2121
BspDI	ATCGAT	1 cut(s)	540
BspFNI	CGCG	3 cut(s)	131, 610, 672
BspLI	GGNNCC	4 cut(s)	629, 714, 885, 1590
BspPI	GGATC	2 cut(s)	1323, 1568
BspT107I	GGYRCC	1 cut(s)	883
BsrDI	GCAATG	5 cut(s)	369, 597, 997, 1121, 1953
BsrGI	TGTACA	1 cut(s)	847
BsrI	ACTGG	1 cut(s)	169
BssECI	CCNNGG	9 cut(s)	21, 81, 228, 452, 617, 888, 914, 1507, 1990
BssMI	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
BssNAI	GTATAC	1 cut(s)	2068
BssNI	GRCGYC	2 cut(s)	9, 144
BssT1I	CCWWGG	7 cut(s)	21, 81, 452, 617, 888, 914, 1507
Bst1107I	GTATAC	1 cut(s)	2068
Bst2UI	CCWGG	1 cut(s)	1833
Bst4CI	ACNGT	6 cut(s)	41, 280, 627, 1423, 1466, 2072
Bst6I	CTCTTC	2 cut(s)	750, 1368
BstACI	GRCGYC	2 cut(s)	9, 144
BstAUI	TGTACA	1 cut(s)	847
BstC8I	GCNNGC	9 cut(s)	7, 367, 398, 459, 670, 1092, 1615, 1766, 2123
BstDEI	CTNAG	9 cut(s)	663, 726, 801, 986, 1147, 1311, 1699, 2028, 2129
BstDSI	CCRYGG	1 cut(s)	617
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	1 cut(s)	662
BstF5I	GGATG	5 cut(s)	238, 362, 1268, 1924, 2144
BstFNI	CGCG	3 cut(s)	131, 610, 672
BstHHI	GCGC	4 cut(s)	328, 610, 865, 1514
BstKTI	GATC	5 cut(s)	1310, 1318, 1563, 1669, 1780
BstMBI	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
BstMWI	GCNNNNNNNGC	6 cut(s)	366, 371, 463, 869, 1112, 1575
BstNI	CCWGG	1 cut(s)	1833
BstNSI	RCATGY	3 cut(s)	854, 1908, 2170
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	4 cut(s)	227, 424, 1831, 1989
BstSFI	CTRYAG	1 cut(s)	972
BstSLI	GKGCMC	3 cut(s)	888, 1333, 2137
BstUI	CGCG	3 cut(s)	131, 610, 672
BstV1I	GCAGC	4 cut(s)	295, 361, 614, 1952
BstV2I	GAAGAC	2 cut(s)	440, 1394
BstX2I	RGATCY	1 cut(s)	1307
BstYI	RGATCY	1 cut(s)	1307
BstZ17I	GTATAC	1 cut(s)	2068
Bsu15I	ATCGAT	1 cut(s)	540
BsuRI	GGCC	5 cut(s)	86, 128, 396, 1578, 2026
BsuTUI	ATCGAT	1 cut(s)	540
BtgI	CCRYGG	1 cut(s)	617
BtsCI	GGATG	5 cut(s)	238, 362, 1268, 1924, 2144
BtsIMutI	CAGTG	1 cut(s)	276
Cac8I	GCNNGC	9 cut(s)	7, 367, 398, 459, 670, 1092, 1615, 1766, 2123
CfoI	GCGC	4 cut(s)	328, 610, 865, 1514
Cfr13I	GGNCC	3 cut(s)	627, 1589, 2024
ClaI	ATCGAT	1 cut(s)	540
CseI	GACGC	1 cut(s)	434
Csp6I	GTAC	4 cut(s)	848, 1043, 1376, 1467
CviQI	GTAC	4 cut(s)	848, 1043, 1376, 1467
DdeI	CTNAG	9 cut(s)	663, 726, 801, 986, 1147, 1311, 1699, 2028, 2129
DpnI	GATC	5 cut(s)	1309, 1317, 1562, 1668, 1779
DpnII	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
DraIII	CACNNNGTG	1 cut(s)	416
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	1 cut(s)	84
Eam1104I	CTCTTC	2 cut(s)	750, 1368
EarI	CTCTTC	2 cut(s)	750, 1368
Eco130I	CCWWGG	7 cut(s)	21, 81, 452, 617, 888, 914, 1507
Eco147I	AGGCCT	1 cut(s)	396
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	2 cut(s)	627, 1589
Eco57I	CTGAAG	5 cut(s)	128, 534, 812, 1016, 1586
Eco91I	GGTNACC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	1 cut(s)	662
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	1 cut(s)	1831
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	7 cut(s)	21, 81, 452, 617, 888, 914, 1507
ErhI	CCWWGG	7 cut(s)	21, 81, 452, 617, 888, 914, 1507
FalI	AAGNNNNNCTT	3 cut(s)	37, 1392, 1424
FaqI	GGGAC	1 cut(s)	613
FauI	CCCGC	1 cut(s)	2146
FblI	GTMKAC	1 cut(s)	2067
Fnu4HI	GCNGC	6 cut(s)	129, 309, 375, 603, 606, 1941
FokI	GGATG	5 cut(s)	245, 369, 1255, 1931, 2131
Fsp4HI	GCNGC	6 cut(s)	129, 309, 375, 603, 606, 1941
FspBI	CTAG	5 cut(s)	392, 690, 1080, 1653, 1809
FspI	TGCGCA	1 cut(s)	864
GlaI	GCGC	4 cut(s)	327, 609, 864, 1513
GluI	GCNGC	6 cut(s)	129, 309, 375, 603, 606, 1941
GsaI	CCCAGC	1 cut(s)	465
GsuI	CTGGAG	2 cut(s)	207, 1892
HaeIII	GGCC	5 cut(s)	86, 128, 396, 1578, 2026
HapII	CCGG	3 cut(s)	228, 425, 1990
HgaI	GACGC	1 cut(s)	434
HhaI	GCGC	4 cut(s)	328, 610, 865, 1514
Hin1I	GRCGYC	2 cut(s)	9, 144
Hin6I	GCGC	4 cut(s)	326, 608, 863, 1512
HinP1I	GCGC	4 cut(s)	326, 608, 863, 1512
HincII	GTYRAC	4 cut(s)	76, 262, 274, 408
HindII	GTYRAC	4 cut(s)	76, 262, 274, 408
HindIII	AAGCTT	2 cut(s)	527, 647
HinfI	GANTC	2 cut(s)	287, 1805
HpaII	CCGG	3 cut(s)	228, 425, 1990
HphI	GGTGA	5 cut(s)	10, 46, 1232, 1674, 1906
Hpy166II	GTNNAC	9 cut(s)	76, 220, 262, 274, 408, 1043, 1223, 2068, 2135
Hpy8I	GTNNAC	9 cut(s)	76, 220, 262, 274, 408, 1043, 1223, 2068, 2135
Hpy99I	CGWCG	3 cut(s)	41, 146, 1328
HpyCH4III	ACNGT	6 cut(s)	41, 280, 627, 1423, 1466, 2072
HpyCH4IV	ACGT	1 cut(s)	144
HpyCH4V	TGCA	6 cut(s)	602, 749, 1251, 1478, 1958, 2135
HpyF10VI	GCNNNNNNNGC	6 cut(s)	366, 371, 463, 869, 1112, 1575
HpyF3I	CTNAG	9 cut(s)	663, 726, 801, 986, 1147, 1311, 1699, 2028, 2129
HpySE526I	ACGT	1 cut(s)	144
Hsp92I	GRCGYC	2 cut(s)	9, 144
HspAI	GCGC	4 cut(s)	326, 608, 863, 1512
Kzo9I	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
LmnI	GCTCC	3 cut(s)	382, 659, 718
Lsp1109I	GCAGC	4 cut(s)	295, 361, 614, 1952
MaeI	CTAG	5 cut(s)	392, 690, 1080, 1653, 1809
MaeII	ACGT	1 cut(s)	144
MaeIII	GTNAC	8 cut(s)	16, 160, 621, 892, 1075, 1460, 1852, 2160
MalI	GATC	5 cut(s)	1309, 1317, 1562, 1668, 1779
MboI	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
MfeI	CAATTG	1 cut(s)	750
MflI	RGATCY	1 cut(s)	1307
MhlI	GDGCHC	3 cut(s)	888, 1333, 2137
MlsI	TGGCCA	1 cut(s)	86
MluNI	TGGCCA	1 cut(s)	86
MlyI	GAGTC	1 cut(s)	1814
MmeI	TCCRAC	5 cut(s)	10, 1273, 1405, 1618, 1621
Mox20I	TGGCCA	1 cut(s)	86
MroXI	GAANNNNTTC	2 cut(s)	181, 1029
MscI	TGGCCA	1 cut(s)	86
MseI	TTAA	4 cut(s)	681, 810, 909, 1128
MslI	CAYNNNNRTG	2 cut(s)	56, 355
Msp20I	TGGCCA	1 cut(s)	86
MspA1I	CMGCKG	1 cut(s)	605
MspI	CCGG	3 cut(s)	228, 425, 1990
MspR9I	CCNGG	4 cut(s)	229, 426, 1833, 1991
MunI	CAATTG	1 cut(s)	750
Mva1269I	GAATGC	1 cut(s)	1490
MvaI	CCWGG	1 cut(s)	1833
MvnI	CGCG	3 cut(s)	131, 610, 672
MwoI	GCNNNNNNNGC	6 cut(s)	366, 371, 463, 869, 1112, 1575
NciI	CCSGG	3 cut(s)	229, 426, 1991
NcoI	CCATGG	1 cut(s)	617
NdeII	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
NlaIV	GGNNCC	4 cut(s)	629, 714, 885, 1590
NmuCI	GTSAC	2 cut(s)	16, 2160
NsbI	TGCGCA	1 cut(s)	864
NspI	RCATGY	3 cut(s)	854, 1908, 2170
PceI	AGGCCT	1 cut(s)	396
PciI	ACATGT	1 cut(s)	850
PcsI	WCGNNNNNNNCGW	1 cut(s)	45
PctI	GAATGC	1 cut(s)	1490
PdmI	GAANNNNTTC	2 cut(s)	181, 1029
PfeI	GAWTC	1 cut(s)	287
PflFI	GACNNNGTC	1 cut(s)	41
PfoI	TCCNGGA	1 cut(s)	424
PkrI	GCNGC	6 cut(s)	130, 310, 376, 604, 607, 1942
PleI	GAGTC	1 cut(s)	1813
PpsI	GAGTC	1 cut(s)	1813
PscI	ACATGT	1 cut(s)	850
Psp6I	CCWGG	1 cut(s)	1831
PspEI	GGTNACC	1 cut(s)	16
PspFI	CCCAGC	1 cut(s)	461
PspGI	CCWGG	1 cut(s)	1831
PspN4I	GGNNCC	4 cut(s)	629, 714, 885, 1590
PspPI	GGNCC	3 cut(s)	627, 1589, 2024
PsrI	GAACNNNNNNTAC	2 cut(s)	987, 1019
PsuI	RGATCY	1 cut(s)	1307
PsyI	GACNNNGTC	1 cut(s)	41
RsaI	GTAC	4 cut(s)	849, 1044, 1377, 1468
RsaNI	GTAC	4 cut(s)	848, 1043, 1376, 1467
RseI	CAYNNNNRTG	2 cut(s)	56, 355
SaqAI	TTAA	4 cut(s)	681, 810, 909, 1128
SatI	GCNGC	6 cut(s)	129, 309, 375, 603, 606, 1941
Sau3AI	GATC	5 cut(s)	1307, 1315, 1560, 1666, 1777
Sau96I	GGNCC	3 cut(s)	627, 1589, 2024
SchI	GAGTC	1 cut(s)	1814
ScrFI	CCNGG	4 cut(s)	229, 426, 1833, 1991
SduI	GDGCHC	3 cut(s)	888, 1333, 2137
SfcI	CTRYAG	1 cut(s)	972
SinI	GGWCC	2 cut(s)	627, 1589
SmiMI	CAYNNNNRTG	2 cut(s)	56, 355
SmlI	CTYRAG	4 cut(s)	98, 558, 1226, 2048
SmoI	CTYRAG	4 cut(s)	98, 558, 1226, 2048
SseBI	AGGCCT	1 cut(s)	396
SsiI	CCGC	4 cut(s)	5, 129, 605, 2139
SspMI	CTAG	5 cut(s)	392, 690, 1080, 1653, 1809
StuI	AGGCCT	1 cut(s)	396
StyD4I	CCNGG	4 cut(s)	227, 424, 1831, 1989
StyI	CCWWGG	7 cut(s)	21, 81, 452, 617, 888, 914, 1507
TaaI	ACNGT	6 cut(s)	41, 280, 627, 1423, 1466, 2072
TaiI	ACGT	1 cut(s)	147
TaqI	TCGA	8 cut(s)	120, 210, 540, 580, 744, 1539, 1692, 1936
TatI	WGTACW	1 cut(s)	847
TauI	GCSGC	2 cut(s)	131, 608
TfiI	GAWTC	1 cut(s)	287
Tru1I	TTAA	4 cut(s)	681, 810, 909, 1128
Tru9I	TTAA	4 cut(s)	681, 810, 909, 1128
TscAI	CASTG	1 cut(s)	283
TseFI	GTSAC	2 cut(s)	16, 2160
TseI	GCWGC	4 cut(s)	308, 374, 602, 1940
Tsp45I	GTSAC	2 cut(s)	16, 2160
TspDTI	ATGAA	3 cut(s)	758, 1767, 1935
TspGWI	ACGGA	1 cut(s)	1393
TspRI	CASTG	1 cut(s)	283
Tth111I	GACNNNGTC	1 cut(s)	41
VneI	GTGCAC	1 cut(s)	2133
VpaK11BI	GGWCC	2 cut(s)	627, 1589
XagI	CCTNNNNNAGG	1 cut(s)	662
XapI	RAATTY	3 cut(s)	1025, 1646, 1866
XbaI	TCTAGA	1 cut(s)	1808
XceI	RCATGY	3 cut(s)	854, 1908, 2170
XmiI	GTMKAC	1 cut(s)	2067
XmnI	GAANNNNTTC	2 cut(s)	181, 1029
XspI	CTAG	5 cut(s)	392, 690, 1080, 1653, 1809
ZraI	GACGTC	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.