AT4G31050

Biotin/lipoate A/B protein ligase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005677
Physical Location & Seq
Forward (+)
15114250 .. 15115633
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31050.1

Sequence Viewer

Length: 837 bp
ATGGAGTTGCTGAATGGAGTAGAAACTCTAGTTTCGGGAATTCATCATCACCATCGTACTAACGCGAAGAGAAACAGATTAGTTAGATCTGTCAAGATCTTAAACTCCGGGAATCATGAAATCCCCAGAAAGTGCCTGTGTTTTGATCTTTACGACAAATTGGTTCCATACAAGAAAGCTTGGTCATGGCAGAAGAGCATTGTCGAGGAGAAAAAGACATTGATTGATAGAAACCAAGACTGCGCTGATACAGTGATTTTGTTACAACACTCTCCTGTTTACACAATGGGAACTGCTAGTACAGAGGATTATCTCAATTTCGATATAAAGGATGCTCCTTTCAATGTTTATCGAACTGAACGTGGTGGCGAAGTCACTTACCATGGCCCTGGTCAGCTAGTTATGTATCCTATAATCAATCTTCGAAATCACGAGATGGATCTTCATTGGTACCTCAGGATGCTTGAAGAGATTGTCATCCGTGTGCTTTCCTCAACTTTCTCCATTAAAGCTTCTCGTCTTGACGGTTTAACTGGAGTTTGGGTTGGCAACCAAAAAGTGGCGGCGATAGGTATTCGTGTGTCGAAATGGATAACATATCATGGTTTAGCTCTTAATGTCACTACAGATCTAACACCATTCAACTGGATAGTTCCATGTGGGATTAGAGACCGGAAAGTTGGAAACATAAAGGGCTTGCTTGAAGATGGAGAACACGGAATGGTTGATGATTTGAGGTTGATCGATATAGTTCACGAGTCTTTACTAAAGGAATTCTCTGAAGCTTTCCAGCTTCAGATCGAAAAGCAAACAGTTTCAGATCCTAATATTCTGTAA

Protein Analysis

278

Amino Acids

31.86

Weight (kDa)

6.87

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 57 - 235 4.5e-42 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 450
AccB7I CCANNNNNTGG 1 cut(s) 559
AccII CGCG 1 cut(s) 65
AciI CCGC 1 cut(s) 563
AclWI GGATC 2 cut(s) 447, 815
AcsI RAATTY 2 cut(s) 39, 773
AcuI CTGAAG 2 cut(s) 779, 801
AfaI GTAC 3 cut(s) 58, 301, 452
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 4 cut(s) 343, 467, 643, 704
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 6 cut(s) 179, 397, 512, 611, 785, 793
AluI AGCT 6 cut(s) 179, 397, 512, 611, 785, 793
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 2 cut(s) 447, 815
AoxI GGCC 1 cut(s) 385
ApoI RAATTY 2 cut(s) 39, 773
Asp718I GGTACC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 41
AsuII TTCGAA 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 455
BanI GGYRCC 1 cut(s) 450
BauI CACGAG 2 cut(s) 431, 755
BccI CCATC 3 cut(s) 60, 430, 701
BciT130I CCWGG 1 cut(s) 390
BciVI GTATCC 1 cut(s) 417
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 3 cut(s) 29, 297, 398
BfmI CTRYAG 1 cut(s) 624
BfuI GTATCC 1 cut(s) 417
BglII AGATCT 3 cut(s) 86, 96, 628
BisI GCNGC 1 cut(s) 564
BlsI GCNGC 1 cut(s) 565
Bme1390I CCNGG 2 cut(s) 109, 390
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 2 cut(s) 165, 452
BmrFI CCNGG 2 cut(s) 109, 390
BmsI GCATC 2 cut(s) 322, 450
BpmI CTGGAG 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 2 cut(s) 382, 388
BsaWI WCCGGW 1 cut(s) 672
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 538, 650
Bse21I CCTNAGG 1 cut(s) 455
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 2 cut(s) 382, 388
BseGI GGATG 3 cut(s) 337, 465, 477
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 469
BseNI ACTGG 2 cut(s) 538, 650
BseRI GAGGAG 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 1 cut(s) 387
BshNI GGYRCC 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 744
BsiSI CCGG 2 cut(s) 108, 673
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 1 cut(s) 663
BsnI GGCC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 663
Bsp119I TTCGAA 1 cut(s) 424
Bsp143I GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 1 cut(s) 563
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 1 cut(s) 468
BspDI ATCGAT 1 cut(s) 744
BspFNI CGCG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 165, 452
BspPI GGATC 2 cut(s) 447, 815
BspQI GCTCTTC 1 cut(s) 188
BspT104I TTCGAA 1 cut(s) 424
BspT107I GGYRCC 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 663
BsrI ACTGG 2 cut(s) 538, 650
BssECI CCNNGG 2 cut(s) 382, 388
BssMI GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
BssSI CACGAG 2 cut(s) 431, 755
BssT1I CCWWGG 1 cut(s) 382
Bst2BI CACGAG 2 cut(s) 431, 755
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 3 cut(s) 253, 527, 814
Bst6I CTCTTC 3 cut(s) 62, 188, 462
BstBI TTCGAA 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 698
BstDEI CTNAG 1 cut(s) 455
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 337, 465, 477
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 8 cut(s) 89, 99, 148, 442, 631, 744, 801, 823
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 107, 388
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 1 cut(s) 65
BstX2I RGATCY 5 cut(s) 86, 96, 439, 628, 820
BstXI CCANNNNNNTGG 2 cut(s) 389, 645
BstYI RGATCY 5 cut(s) 86, 96, 439, 628, 820
Bsu15I ATCGAT 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 455
BsuI GTATCC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 387
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 337, 465, 477
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 698
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 386
ClaI ATCGAT 1 cut(s) 744
Csp6I GTAC 3 cut(s) 57, 300, 451
CviAII CATG 5 cut(s) 116, 186, 383, 602, 657
CviJI RGCY 8 cut(s) 179, 387, 397, 512, 611, 696, 785, 793
CviKI_1 RGCY 8 cut(s) 179, 387, 397, 512, 611, 696, 785, 793
CviQI GTAC 3 cut(s) 57, 300, 451
DdeI CTNAG 1 cut(s) 455
DpnI GATC 8 cut(s) 88, 98, 147, 441, 630, 743, 800, 822
DpnII GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
Eam1104I CTCTTC 3 cut(s) 62, 188, 462
EarI CTCTTC 3 cut(s) 62, 188, 462
Eco130I CCWWGG 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 663
Eco57I CTGAAG 2 cut(s) 779, 801
Eco81I CCTNAGG 1 cut(s) 455
EcoRI GAATTC 2 cut(s) 39, 773
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 5 cut(s) 119, 189, 386, 605, 660
FatI CATG 5 cut(s) 115, 185, 382, 601, 656
Fnu4HI GCNGC 1 cut(s) 564
FokI GGATG 3 cut(s) 344, 464, 472
Fsp4HI GCNGC 1 cut(s) 564
FspBI CTAG 3 cut(s) 29, 297, 398
GlaI GCGC 1 cut(s) 244
GluI GCNGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 108, 673
HhaI GCGC 1 cut(s) 245
Hin1II CATG 5 cut(s) 119, 189, 386, 605, 660
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HindIII AAGCTT 3 cut(s) 177, 510, 783
HinfI GANTC 2 cut(s) 112, 758
HpaII CCGG 2 cut(s) 108, 673
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 280, 754
Hpy188I TCNGA 3 cut(s) 781, 798, 820
Hpy188III TCNNGA 7 cut(s) 36, 94, 116, 431, 457, 521, 755
Hpy8I GTNNAC 2 cut(s) 280, 754
HpyCH4III ACNGT 3 cut(s) 253, 527, 814
HpyCH4IV ACGT 1 cut(s) 361
HpyF3I CTNAG 1 cut(s) 455
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 5 cut(s) 119, 189, 386, 605, 660
HspAI GCGC 1 cut(s) 243
KpnI GGTACC 1 cut(s) 454
Kzo9I GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 1 cut(s) 340
LweI GCATC 2 cut(s) 322, 450
MaeI CTAG 3 cut(s) 29, 297, 398
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 3 cut(s) 261, 373, 619
MalI GATC 8 cut(s) 88, 98, 147, 441, 630, 743, 800, 822
MboI GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
MboII GAAGA 6 cut(s) 79, 205, 413, 434, 479, 716
MflI RGATCY 5 cut(s) 86, 96, 439, 628, 820
MluCI AATT 4 cut(s) 39, 158, 316, 773
MlyI GAGTC 1 cut(s) 767
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 5 cut(s) 199, 298, 464, 502, 729
MseI TTAA 4 cut(s) 101, 507, 530, 615
MslI CAYNNNNRTG 1 cut(s) 482
MspI CCGG 2 cut(s) 108, 673
MspR9I CCNGG 2 cut(s) 109, 390
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 1 cut(s) 65
NciI CCSGG 1 cut(s) 109
NcoI CCATGG 1 cut(s) 382
NdeII GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
NlaIII CATG 5 cut(s) 119, 189, 386, 605, 660
NlaIV GGNNCC 2 cut(s) 165, 452
NmuCI GTSAC 2 cut(s) 373, 619
NspV TTCGAA 1 cut(s) 424
PagI TCATGA 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 358
PfeI GAWTC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 559
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 1 cut(s) 565
PleI GAGTC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 766
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 165, 452
PspPI GGNCC 1 cut(s) 386
PsrI GAACNNNNNNTAC 2 cut(s) 283, 315
PsuI RGATCY 5 cut(s) 86, 96, 439, 628, 820
RsaI GTAC 3 cut(s) 58, 301, 452
RsaNI GTAC 3 cut(s) 57, 300, 451
RseI CAYNNNNRTG 1 cut(s) 482
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 4 cut(s) 101, 507, 530, 615
SatI GCNGC 1 cut(s) 564
Sau3AI GATC 8 cut(s) 86, 96, 145, 439, 628, 741, 798, 820
Sau96I GGNCC 1 cut(s) 386
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 2 cut(s) 109, 390
SfaNI GCATC 2 cut(s) 322, 450
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 4 cut(s) 39, 158, 316, 773
SsiI CCGC 1 cut(s) 563
SspI AATATT 1 cut(s) 829
SspMI CTAG 3 cut(s) 29, 297, 398
StyD4I CCNGG 2 cut(s) 107, 388
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 3 cut(s) 253, 527, 814
TaiI ACGT 1 cut(s) 364
TaqI TCGA 7 cut(s) 204, 321, 352, 424, 584, 744, 801
TasI AATT 4 cut(s) 39, 158, 316, 773
TatI WGTACW 1 cut(s) 299
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 4 cut(s) 101, 507, 530, 615
Tru9I TTAA 4 cut(s) 101, 507, 530, 615
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 2 cut(s) 373, 619
Tsp45I GTSAC 2 cut(s) 373, 619
TspDTI ATGAA 3 cut(s) 32, 132, 434
TspGWI ACGGA 2 cut(s) 470, 732
TspRI CASTG 1 cut(s) 258
Van91I CCANNNNNTGG 1 cut(s) 559
XapI RAATTY 2 cut(s) 39, 773
XspI CTAG 3 cut(s) 29, 297, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.