RchiOBHm_Chr5g0074221

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005677
Physical Location & Seq
Forward (+)
80186206 .. 80189230
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34904

Sequence Viewer

Length: 927 bp
ATGACTTTCTTGGCGACCCAATGTGTCATCGCCTCAATCCCAACACACCCAGTTTCTAATCATCAGCCCAAACGGTGCCTCGCCCAGAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTACTCGGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACCGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGAAAGGAATCAGGACTGCCCGGACTCGCTGTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTACCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCATCGGACCGAACGCGGCGGCGAGGTTACATATCACGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCGATCAAGGCATCTCAACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACAGATTTAACTCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGAGTCATTTGACAATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTACTTGATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTTAGAGTTTTTAGAAAGTGAACCGGTAAAGCCTCTAAAAGAGGAAGTAATTTAG

Protein Analysis

308

Amino Acids

35.24

Weight (kDa)

6.76

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 65 - 244 4.6e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 320, 474
AccB1I GGYRCC 4 cut(s) 75, 314, 320, 474
AccB7I CCANNNNNTGG 1 cut(s) 583
AccI GTMKAC 1 cut(s) 598
AccII CGCG 2 cut(s) 387, 507
AciI CCGC 2 cut(s) 387, 390
AclWI GGATC 2 cut(s) 471, 767
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 5 cut(s) 176, 322, 431, 476, 678
AfiI CCNNNNNNNGG 3 cut(s) 583, 782, 870
AgeI ACCGGT 2 cut(s) 317, 895
AgsI TTSAA 3 cut(s) 169, 251, 516
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 6 cut(s) 120, 184, 203, 620, 766, 790
AluI AGCT 6 cut(s) 120, 184, 203, 620, 766, 790
Alw26I GTCTC 1 cut(s) 612
AlwI GGATC 2 cut(s) 471, 767
AlwNI CAGNNNCTG 2 cut(s) 583, 776
Ama87I CYCGRG 2 cut(s) 132, 868
AoxI GGCC 1 cut(s) 784
ApeKI GCWGC 1 cut(s) 587
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AsiGI ACCGGT 2 cut(s) 317, 895
Asp700I GAANNNNTTC 1 cut(s) 837
Asp718I GGTACC 2 cut(s) 320, 474
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 378, 410
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 508
AvaI CYCGRG 2 cut(s) 132, 868
AvaII GGWCC 2 cut(s) 378, 410
AxyI CCTNAGG 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 317
BalI TGGCCA 1 cut(s) 786
BanI GGYRCC 4 cut(s) 75, 314, 320, 474
BbvI GCAGC 1 cut(s) 599
BccI CCATC 2 cut(s) 776, 872
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 2 cut(s) 498, 816
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 1 cut(s) 185
BisI GCNGC 3 cut(s) 388, 391, 588
BlsI GCNGC 3 cut(s) 389, 392, 589
Bme1390I CCNGG 2 cut(s) 269, 414
Bme18I GGWCC 2 cut(s) 378, 410
BmeT110I CYCGRG 2 cut(s) 132, 868
BmgT120I GGNCC 2 cut(s) 378, 410
BmiI GGNNCC 4 cut(s) 77, 316, 322, 476
BmrFI CCNGG 2 cut(s) 269, 414
BmrI ACTGGG 2 cut(s) 44, 89
BmsI GCATC 3 cut(s) 301, 346, 545
BmuI ACTGGG 2 cut(s) 44, 89
BpuEI CTTGAG 3 cut(s) 335, 509, 566
BpuMI CCSGG 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 372
BsaWI WCCGGW 2 cut(s) 317, 895
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 3 cut(s) 583, 782, 870
Bse118I RCCGGY 3 cut(s) 195, 317, 895
Bse1I ACTGG 4 cut(s) 50, 95, 562, 588
Bse21I CCTNAGG 1 cut(s) 479
Bse8I GATNNNNATC 1 cut(s) 372
BseBI CCWGG 1 cut(s) 414
BseGI GGATG 2 cut(s) 292, 361
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 583, 782, 870
BseMII CTCAG 4 cut(s) 144, 339, 493, 623
BseNI ACTGG 4 cut(s) 50, 95, 562, 588
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 1 cut(s) 599
Bsh1236I CGCG 2 cut(s) 387, 507
BshFI GGCC 1 cut(s) 786
BshNI GGYRCC 4 cut(s) 75, 314, 320, 474
BshTI ACCGGT 2 cut(s) 317, 895
BsiHKCI CYCGRG 2 cut(s) 132, 868
BsiSI CCGG 4 cut(s) 196, 269, 318, 896
BslI CCNNNNNNNGG 3 cut(s) 583, 782, 870
BsmAI GTCTC 1 cut(s) 612
BsnI GGCC 1 cut(s) 786
BsoBI CYCGRG 2 cut(s) 132, 868
Bsp1286I GDGCHC 1 cut(s) 317
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 5 cut(s) 463, 498, 528, 772, 816
BspACI CCGC 2 cut(s) 387, 390
BspANI GGCC 1 cut(s) 786
BspCNI CTCAG 4 cut(s) 145, 338, 492, 622
BspFNI CGCG 2 cut(s) 387, 507
BspLI GGNNCC 4 cut(s) 77, 316, 322, 476
BspPI GGATC 2 cut(s) 471, 767
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 4 cut(s) 75, 314, 320, 474
BsrFI RCCGGY 3 cut(s) 195, 317, 895
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 4 cut(s) 50, 95, 562, 588
BssAI RCCGGY 3 cut(s) 195, 317, 895
BssMI GATC 5 cut(s) 463, 498, 528, 772, 816
BssNAI GTATAC 1 cut(s) 599
Bst1107I GTATAC 1 cut(s) 599
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 5 cut(s) 75, 114, 410, 629, 681
Bst6I CTCTTC 2 cut(s) 139, 212
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 201, 788
BstDEI CTNAG 4 cut(s) 153, 325, 479, 609
BstF5I GGATG 2 cut(s) 292, 361
BstFNI CGCG 2 cut(s) 387, 507
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 5 cut(s) 466, 501, 531, 775, 819
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 5 cut(s) 463, 498, 528, 772, 816
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 2 cut(s) 267, 412
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 2 cut(s) 387, 507
BstV1I GCAGC 1 cut(s) 599
BstX2I RGATCY 2 cut(s) 463, 772
BstYI RGATCY 2 cut(s) 463, 772
BstZ17I GTATAC 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 2 cut(s) 292, 361
BtsIMutI CAGTG 2 cut(s) 617, 635
Cac8I GCNNGC 2 cut(s) 201, 788
CaiI CAGNNNCTG 2 cut(s) 583, 776
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 3 cut(s) 195, 317, 895
Cfr13I GGNCC 2 cut(s) 378, 410
CpoI CGGWCCG 1 cut(s) 378
Csp6I GTAC 5 cut(s) 175, 321, 430, 475, 677
CspAI ACCGGT 2 cut(s) 317, 895
CspI CGGWCCG 1 cut(s) 378
CviAII CATG 1 cut(s) 569
CviQI GTAC 5 cut(s) 175, 321, 430, 475, 677
DdeI CTNAG 4 cut(s) 153, 325, 479, 609
DpnI GATC 5 cut(s) 465, 500, 530, 774, 818
DpnII GATC 5 cut(s) 463, 498, 528, 772, 816
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 2 cut(s) 139, 212
EarI CTCTTC 2 cut(s) 139, 212
Eco47I GGWCC 2 cut(s) 378, 410
Eco57I CTGAAG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 479
Eco88I CYCGRG 2 cut(s) 132, 868
EcoRII CCWGG 1 cut(s) 412
FaeI CATG 1 cut(s) 572
FaiI YATR 6 cut(s) 306, 403, 440, 570, 599, 858
FatI CATG 1 cut(s) 568
FbaI TGATCA 2 cut(s) 498, 816
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 388, 391, 588
FokI GGATG 2 cut(s) 279, 368
Fsp4HI GCNGC 3 cut(s) 388, 391, 588
FspBI CTAG 1 cut(s) 185
GlaI GCGC 1 cut(s) 508
GluI GCNGC 3 cut(s) 388, 391, 588
HaeIII GGCC 1 cut(s) 786
HapII CCGG 4 cut(s) 196, 269, 318, 896
HhaI GCGC 1 cut(s) 509
Hin1II CATG 1 cut(s) 572
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 8 cut(s) 90, 137, 149, 256, 272, 442, 737, 849
HpaII CCGG 4 cut(s) 196, 269, 318, 896
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 3 cut(s) 599, 689, 893
Hpy188I TCNGA 7 cut(s) 154, 246, 328, 378, 420, 449, 832
Hpy188III TCNNGA 5 cut(s) 134, 141, 260, 776, 868
Hpy8I GTNNAC 3 cut(s) 599, 689, 893
HpyCH4III ACNGT 5 cut(s) 75, 114, 410, 629, 681
HpyCH4IV ACGT 1 cut(s) 642
HpyF3I CTNAG 4 cut(s) 153, 325, 479, 609
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 1 cut(s) 572
HspAI GCGC 1 cut(s) 507
KpnI GGTACC 2 cut(s) 324, 478
Ksp22I TGATCA 2 cut(s) 498, 816
Kzo9I GATC 5 cut(s) 463, 498, 528, 772, 816
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 2 cut(s) 181, 364
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 3 cut(s) 301, 346, 545
MaeI CTAG 1 cut(s) 185
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 397, 483, 643
MalI GATC 5 cut(s) 465, 500, 530, 774, 818
MboI GATC 5 cut(s) 463, 498, 528, 772, 816
MboII GAAGA 4 cut(s) 156, 229, 278, 458
MflI RGATCY 2 cut(s) 463, 772
MhlI GDGCHC 1 cut(s) 317
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 3 cut(s) 340, 853, 921
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 3 cut(s) 158, 266, 746
MmeI TCCRAC 4 cut(s) 149, 650, 685, 826
Mox20I TGGCCA 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 1 cut(s) 786
MseI TTAA 3 cut(s) 102, 554, 656
Msp20I TGGCCA 1 cut(s) 786
MspI CCGG 4 cut(s) 196, 269, 318, 896
MspR9I CCNGG 2 cut(s) 269, 414
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 2 cut(s) 387, 507
NciI CCSGG 1 cut(s) 269
NdeII GATC 5 cut(s) 463, 498, 528, 772, 816
NlaIII CATG 1 cut(s) 572
NlaIV GGNNCC 4 cut(s) 77, 316, 322, 476
NmuCI GTSAC 1 cut(s) 483
PaeR7I CTCGAG 1 cut(s) 868
PciSI GCTCTTC 1 cut(s) 212
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 5 cut(s) 90, 137, 256, 442, 849
PflMI CCANNNNNTGG 1 cut(s) 583
PinAI ACCGGT 2 cut(s) 317, 895
PkrI GCNGC 3 cut(s) 389, 392, 589
PleI GAGTC 3 cut(s) 157, 266, 745
PpsI GAGTC 3 cut(s) 157, 266, 745
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 4 cut(s) 77, 316, 322, 476
PspPI GGNCC 2 cut(s) 378, 410
PstNI CAGNNNCTG 2 cut(s) 583, 776
PsuI RGATCY 2 cut(s) 463, 772
RsaI GTAC 5 cut(s) 176, 322, 431, 476, 678
RsaNI GTAC 5 cut(s) 175, 321, 430, 475, 677
Rsr2I CGGWCCG 1 cut(s) 378
RsrII CGGWCCG 1 cut(s) 378
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 102, 554, 656
SatI GCNGC 3 cut(s) 388, 391, 588
Sau3AI GATC 5 cut(s) 463, 498, 528, 772, 816
Sau96I GGNCC 2 cut(s) 378, 410
SchI GAGTC 3 cut(s) 158, 266, 746
ScrFI CCNGG 2 cut(s) 269, 414
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 3 cut(s) 301, 346, 545
Sfr274I CTCGAG 1 cut(s) 868
SinI GGWCC 2 cut(s) 378, 410
SlaI CTCGAG 1 cut(s) 868
SmlI CTYRAG 4 cut(s) 350, 488, 545, 868
SmoI CTYRAG 4 cut(s) 350, 488, 545, 868
Sse9I AATT 3 cut(s) 340, 853, 921
SsiI CCGC 2 cut(s) 387, 390
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 267, 412
TaaI ACNGT 5 cut(s) 75, 114, 410, 629, 681
TaiI ACGT 1 cut(s) 645
TaqI TCGA 4 cut(s) 116, 140, 527, 869
TaqII GACCGA 1 cut(s) 395
TasI AATT 3 cut(s) 340, 853, 921
TatI WGTACW 1 cut(s) 174
TauI GCSGC 2 cut(s) 390, 393
TfiI GAWTC 5 cut(s) 90, 137, 256, 442, 849
Tru1I TTAA 3 cut(s) 102, 554, 656
Tru9I TTAA 3 cut(s) 102, 554, 656
TscAI CASTG 2 cut(s) 617, 642
TseFI GTSAC 1 cut(s) 483
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 4 cut(s) 362, 455, 458, 873
TspRI CASTG 2 cut(s) 617, 642
Van91I CCANNNNNTGG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 378, 410
XhoI CTCGAG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 598
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.