Rw6G031150

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005677
Physical Location & Seq
Reverse (-)
55177396 .. 55182306
4911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031150.1

Sequence Viewer

Length: 1002 bp
ATGACTTTCTTGGCGACCCAATGTGTCGTCGCCTCAATCCCAGCATACCCAGTTTGTAATCATCAGCCCAAACCTCCTGGGTGCCTCTCCCAGAGAATCCAATCGAAATCGGACAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTAGTCAGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCGTACAGCCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGAGAGGAACGAGGACTGCCCCGACTCGCTGTTCGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACGTCAAGGATGCTCCGTTTGATGTTCACCGGACCGAACGCGGCGGCGAGGTTACATATCATGGTCCTGGTCAGATAGTAATGTACCCCATTATAAATCTCCGGAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCGATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACAGATTTAACTCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTCGGAAGCATTAGAGGGTTGTTAGGGGAGTTTGAGTCACTTGACGATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTACTTAATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTTAGAGTTTTTAGAAATGAGCAAAACTAAAATGAAAACTGAAAAAATTCAAACTTCTGATTTAAAATACATGAAAATTGGCATTTTGATACAGTTCTTATAA

Protein Analysis

333

Amino Acids

37.95

Weight (kDa)

8.04

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 74 - 256 6.4e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 467, 1000
AatII GACGTC 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 501
AccB1I GGYRCC 3 cut(s) 81, 341, 501
AccB7I CCANNNNNTGG 1 cut(s) 610
AccI GTMKAC 1 cut(s) 625
AccII CGCG 2 cut(s) 414, 534
AccIII TCCGGA 1 cut(s) 474
AciI CCGC 2 cut(s) 414, 417
AclWI GGATC 2 cut(s) 498, 794
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 945
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 5 cut(s) 203, 221, 458, 503, 705
AfiI CCNNNNNNNGG 3 cut(s) 610, 809, 897
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 3 cut(s) 196, 543, 950
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 2 cut(s) 76, 439
AluBI AGCT 7 cut(s) 147, 211, 230, 351, 647, 793, 817
AluI AGCT 7 cut(s) 147, 211, 230, 351, 647, 793, 817
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 2 cut(s) 498, 794
AlwNI CAGNNNCTG 2 cut(s) 610, 803
Ama87I CYCGRG 1 cut(s) 895
Aor13HI TCCGGA 1 cut(s) 474
AoxI GGCC 1 cut(s) 811
ApeKI GCWGC 1 cut(s) 614
ApoI RAATTY 1 cut(s) 945
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 2 cut(s) 864, 945
Asp718I GGTACC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 2 cut(s) 405, 437
AsuHPI GGTGA 2 cut(s) 392, 535
AvaI CYCGRG 1 cut(s) 895
AvaII GGWCC 2 cut(s) 405, 437
AxyI CCTNAGG 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 344
BalI TGGCCA 1 cut(s) 813
BanI GGYRCC 3 cut(s) 81, 341, 501
BbvI GCAGC 1 cut(s) 626
BccI CCATC 2 cut(s) 803, 899
BciT130I CCWGG 2 cut(s) 78, 441
BclI TGATCA 2 cut(s) 525, 843
BcoDI GTCTC 1 cut(s) 639
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 2 cut(s) 156, 212
BisI GCNGC 3 cut(s) 415, 418, 615
BlsI GCNGC 3 cut(s) 416, 419, 616
Bme1390I CCNGG 2 cut(s) 78, 441
Bme18I GGWCC 2 cut(s) 405, 437
BmeT110I CYCGRG 1 cut(s) 895
BmgT120I GGNCC 2 cut(s) 405, 437
BmiI GGNNCC 3 cut(s) 83, 343, 503
BmrFI CCNGG 2 cut(s) 78, 441
BmrI ACTGGG 2 cut(s) 44, 116
BmsI GCATC 3 cut(s) 328, 373, 572
BmuI ACTGGG 2 cut(s) 44, 116
BpuEI CTTGAG 2 cut(s) 536, 593
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 3 cut(s) 344, 402, 474
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 3 cut(s) 610, 809, 897
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 4 cut(s) 50, 122, 589, 615
Bse21I CCTNAGG 1 cut(s) 506
BseAI TCCGGA 1 cut(s) 474
BseBI CCWGG 2 cut(s) 78, 441
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 319, 388
BseLI CCNNNNNNNGG 3 cut(s) 610, 809, 897
BseMII CTCAG 4 cut(s) 171, 366, 520, 650
BseNI ACTGG 4 cut(s) 50, 122, 589, 615
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 1 cut(s) 626
BseYI CCCAGC 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 414, 534
BshFI GGCC 1 cut(s) 813
BshNI GGYRCC 3 cut(s) 81, 341, 501
BshTI ACCGGT 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 895
BsiSI CCGG 3 cut(s) 345, 403, 475
BslI CCNNNNNNNGG 3 cut(s) 610, 809, 897
BsmAI GTCTC 1 cut(s) 639
BsnI GGCC 1 cut(s) 813
BsoBI CYCGRG 1 cut(s) 895
Bsp1286I GDGCHC 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 474
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 5 cut(s) 490, 525, 555, 799, 843
BspACI CCGC 2 cut(s) 414, 417
BspANI GGCC 1 cut(s) 813
BspCNI CTCAG 4 cut(s) 172, 365, 519, 649
BspEI TCCGGA 1 cut(s) 474
BspFNI CGCG 2 cut(s) 414, 534
BspLI GGNNCC 3 cut(s) 83, 343, 503
BspPI GGATC 2 cut(s) 498, 794
BspQI GCTCTTC 1 cut(s) 239
BspT107I GGYRCC 3 cut(s) 81, 341, 501
BsrFI RCCGGY 1 cut(s) 344
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 4 cut(s) 50, 122, 589, 615
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 5 cut(s) 490, 525, 555, 799, 843
BssNAI GTATAC 1 cut(s) 626
BssNI GRCGYC 1 cut(s) 375
Bst1107I GTATAC 1 cut(s) 626
Bst2UI CCWGG 2 cut(s) 78, 441
Bst4CI ACNGT 4 cut(s) 141, 656, 708, 993
Bst6I CTCTTC 2 cut(s) 166, 239
BstACI GRCGYC 1 cut(s) 375
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 815
BstDEI CTNAG 4 cut(s) 180, 352, 506, 636
BstF5I GGATG 2 cut(s) 319, 388
BstFNI CGCG 2 cut(s) 414, 534
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 5 cut(s) 493, 528, 558, 802, 846
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 5 cut(s) 490, 525, 555, 799, 843
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNI CCWGG 2 cut(s) 78, 441
BstSCI CCNGG 2 cut(s) 76, 439
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 2 cut(s) 414, 534
BstV1I GCAGC 1 cut(s) 626
BstX2I RGATCY 2 cut(s) 490, 799
BstYI RGATCY 2 cut(s) 490, 799
BstZ17I GTATAC 1 cut(s) 626
Bsu36I CCTNAGG 1 cut(s) 506
BsuRI GGCC 1 cut(s) 813
BtsCI GGATG 2 cut(s) 319, 388
BtsIMutI CAGTG 2 cut(s) 644, 662
Cac8I GCNNGC 1 cut(s) 815
CaiI CAGNNNCTG 2 cut(s) 610, 803
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 405, 437
CpoI CGGWCCG 1 cut(s) 405
Csp6I GTAC 5 cut(s) 202, 220, 457, 502, 704
CspAI ACCGGT 1 cut(s) 344
CspI CGGWCCG 1 cut(s) 405
CviAII CATG 3 cut(s) 434, 596, 970
CviQI GTAC 5 cut(s) 202, 220, 457, 502, 704
DdeI CTNAG 4 cut(s) 180, 352, 506, 636
DpnI GATC 5 cut(s) 492, 527, 557, 801, 845
DpnII GATC 5 cut(s) 490, 525, 555, 799, 843
DraI TTTAAA 1 cut(s) 963
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 2 cut(s) 166, 239
EarI CTCTTC 2 cut(s) 166, 239
Eco47I GGWCC 2 cut(s) 405, 437
Eco57I CTGAAG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 895
EcoRII CCWGG 2 cut(s) 76, 439
FaeI CATG 3 cut(s) 437, 599, 973
FatI CATG 3 cut(s) 433, 595, 969
FbaI TGATCA 2 cut(s) 525, 843
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 3 cut(s) 415, 418, 615
FokI GGATG 2 cut(s) 306, 395
Fsp4HI GCNGC 3 cut(s) 415, 418, 615
FspBI CTAG 2 cut(s) 156, 212
GlaI GCGC 1 cut(s) 535
GluI GCNGC 3 cut(s) 415, 418, 615
GsaI CCCAGC 1 cut(s) 44
HaeIII GGCC 1 cut(s) 813
HapII CCGG 3 cut(s) 345, 403, 475
HhaI GCGC 1 cut(s) 536
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 3 cut(s) 437, 599, 973
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 8 cut(s) 96, 117, 164, 176, 299, 478, 764, 876
HpaII CCGG 3 cut(s) 345, 403, 475
HphI GGTGA 2 cut(s) 392, 535
Hpy166II GTNNAC 3 cut(s) 400, 626, 716
Hpy188I TCNGA 8 cut(s) 112, 181, 273, 355, 447, 734, 859, 958
Hpy188III TCNNGA 6 cut(s) 161, 168, 475, 567, 803, 895
Hpy8I GTNNAC 3 cut(s) 400, 626, 716
Hpy99I CGWCG 1 cut(s) 32
HpyCH4III ACNGT 4 cut(s) 141, 656, 708, 993
HpyCH4IV ACGT 2 cut(s) 375, 669
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpyF3I CTNAG 4 cut(s) 180, 352, 506, 636
HpySE526I ACGT 2 cut(s) 375, 669
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 3 cut(s) 437, 599, 973
HspAI GCGC 1 cut(s) 534
Kpn2I TCCGGA 1 cut(s) 474
KpnI GGTACC 1 cut(s) 505
Ksp22I TGATCA 2 cut(s) 525, 843
Kzo9I GATC 5 cut(s) 490, 525, 555, 799, 843
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 2 cut(s) 208, 391
Lsp1109I GCAGC 1 cut(s) 626
LweI GCATC 3 cut(s) 328, 373, 572
MaeI CTAG 2 cut(s) 156, 212
MaeII ACGT 2 cut(s) 375, 669
MaeIII GTNAC 4 cut(s) 424, 510, 670, 765
MalI GATC 5 cut(s) 492, 527, 557, 801, 845
MboI GATC 5 cut(s) 490, 525, 555, 799, 843
MboII GAAGA 4 cut(s) 183, 256, 305, 485
MflI RGATCY 2 cut(s) 490, 799
MhlI GDGCHC 1 cut(s) 344
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 4 cut(s) 367, 880, 945, 975
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 3 cut(s) 185, 293, 773
MmeI TCCRAC 4 cut(s) 176, 677, 712, 853
Mox20I TGGCCA 1 cut(s) 813
MroI TCCGGA 1 cut(s) 474
MroXI GAANNNNTTC 2 cut(s) 864, 945
MscI TGGCCA 1 cut(s) 813
MseI TTAA 5 cut(s) 129, 581, 683, 822, 962
Msp20I TGGCCA 1 cut(s) 813
MspI CCGG 3 cut(s) 345, 403, 475
MspR9I CCNGG 2 cut(s) 78, 441
MvaI CCWGG 2 cut(s) 78, 441
MvnI CGCG 2 cut(s) 414, 534
MwoI GCNNNNNNNGC 1 cut(s) 348
NdeII GATC 5 cut(s) 490, 525, 555, 799, 843
NlaIII CATG 3 cut(s) 437, 599, 973
NlaIV GGNNCC 3 cut(s) 83, 343, 503
NmuCI GTSAC 2 cut(s) 510, 765
PaeR7I CTCGAG 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 864, 945
PfeI GAWTC 5 cut(s) 96, 117, 164, 478, 876
PflMI CCANNNNNTGG 1 cut(s) 610
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 3 cut(s) 416, 419, 616
PleI GAGTC 3 cut(s) 184, 293, 772
PpsI GAGTC 3 cut(s) 184, 293, 772
PsiI TTATAA 2 cut(s) 467, 1000
Psp6I CCWGG 2 cut(s) 76, 439
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 2 cut(s) 76, 439
PspN4I GGNNCC 3 cut(s) 83, 343, 503
PspPI GGNCC 2 cut(s) 405, 437
PstNI CAGNNNCTG 2 cut(s) 610, 803
PsuI RGATCY 2 cut(s) 490, 799
RsaI GTAC 5 cut(s) 203, 221, 458, 503, 705
RsaNI GTAC 5 cut(s) 202, 220, 457, 502, 704
Rsr2I CGGWCCG 1 cut(s) 405
RsrII CGGWCCG 1 cut(s) 405
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 5 cut(s) 129, 581, 683, 822, 962
SatI GCNGC 3 cut(s) 415, 418, 615
Sau3AI GATC 5 cut(s) 490, 525, 555, 799, 843
Sau96I GGNCC 2 cut(s) 405, 437
SchI GAGTC 3 cut(s) 185, 293, 773
ScrFI CCNGG 2 cut(s) 78, 441
SduI GDGCHC 1 cut(s) 344
SfaNI GCATC 3 cut(s) 328, 373, 572
Sfr274I CTCGAG 1 cut(s) 895
SinI GGWCC 2 cut(s) 405, 437
SlaI CTCGAG 1 cut(s) 895
SmlI CTYRAG 3 cut(s) 515, 572, 895
SmoI CTYRAG 3 cut(s) 515, 572, 895
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 4 cut(s) 367, 880, 945, 975
SsiI CCGC 2 cut(s) 414, 417
SspI AATATT 1 cut(s) 826
SspMI CTAG 2 cut(s) 156, 212
StyD4I CCNGG 2 cut(s) 76, 439
TaaI ACNGT 4 cut(s) 141, 656, 708, 993
TaiI ACGT 2 cut(s) 378, 672
TaqI TCGA 6 cut(s) 104, 143, 167, 554, 568, 896
TaqII GACCGA 1 cut(s) 422
TasI AATT 4 cut(s) 367, 880, 945, 975
TatI WGTACW 1 cut(s) 201
TauI GCSGC 2 cut(s) 417, 420
TfiI GAWTC 5 cut(s) 96, 117, 164, 478, 876
Tru1I TTAA 5 cut(s) 129, 581, 683, 822, 962
Tru9I TTAA 5 cut(s) 129, 581, 683, 822, 962
TscAI CASTG 2 cut(s) 644, 669
TseFI GTSAC 2 cut(s) 510, 765
TseI GCWGC 1 cut(s) 614
Tsp45I GTSAC 2 cut(s) 510, 765
TspDTI ATGAA 4 cut(s) 485, 900, 947, 986
TspGWI ACGGA 2 cut(s) 207, 378
TspRI CASTG 2 cut(s) 644, 669
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 2 cut(s) 405, 437
XapI RAATTY 1 cut(s) 945
XhoI CTCGAG 1 cut(s) 895
XmiI GTMKAC 1 cut(s) 625
XmnI GAANNNNTTC 2 cut(s) 864, 945
XspI CTAG 2 cut(s) 156, 212
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.